FR2366571A1 - Procede de determination quantitative d'antigenes ou d'anticorps - Google Patents

Procede de determination quantitative d'antigenes ou d'anticorps

Info

Publication number
FR2366571A1
FR2366571A1 FR7729514A FR7729514A FR2366571A1 FR 2366571 A1 FR2366571 A1 FR 2366571A1 FR 7729514 A FR7729514 A FR 7729514A FR 7729514 A FR7729514 A FR 7729514A FR 2366571 A1 FR2366571 A1 FR 2366571A1
Authority
FR
France
Prior art keywords
antigen
antibody
hemagglutination
curve
antigens
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
FR7729514A
Other languages
English (en)
Inventor
Tom Bertil Bjorklund
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bonnierforetagen AB
Original Assignee
Bonnierforetagen AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bonnierforetagen AB filed Critical Bonnierforetagen AB
Publication of FR2366571A1 publication Critical patent/FR2366571A1/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306Solid-phase reaction mechanisms
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N21/82Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a precipitate or turbidity
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00Chemistry: analytical and immunological testing
    • Y10S436/805Optical property

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Hematology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Plasma & Fusion (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Procédé de détermination quantitative de la quantité d'antigène ou d'anticorps dans une solution en appliquant la technique d'inhibition de l'hémagglutination ou de l'hémagglutina-tion directe. On mesure les diamètres des dépôts d'hémagglutination d'échantillons témoins et on porte les valeurs en fonction des concentrations en antigène ou en anticorps; on obtient une courbe en S dont la portion médiane présente une forte pente. Par le choix d'un échantillon dans la série des échantillons de concentration inconnue en antigène ou en anticorps ayant un diamètre situé dans la portion médiane de la courbe et à l'aide de ce diamètre, on peut déterminer d'après la courbe la concentration en antigène ou en anticorps. Le procédé assure une précision très élevée.
FR7729514A 1976-10-01 1977-09-30 Procede de determination quantitative d'antigenes ou d'anticorps Withdrawn FR2366571A1 (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE7610939A SE7610939L (sv) 1976-10-01 1976-10-01 Forfarande for kvantitativ bestemning av antigen eller antikropp

Publications (1)

Publication Number Publication Date
FR2366571A1 true FR2366571A1 (fr) 1978-04-28

Family

ID=20329024

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7729514A Withdrawn FR2366571A1 (fr) 1976-10-01 1977-09-30 Procede de determination quantitative d'antigenes ou d'anticorps

Country Status (6)

Country Link
US (1) US4193981A (fr)
JP (1) JPS5379025A (fr)
DE (1) DE2744028A1 (fr)
FR (1) FR2366571A1 (fr)
GB (1) GB1549921A (fr)
SE (1) SE7610939L (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0165551A2 (fr) * 1984-06-19 1985-12-27 BEHRINGWERKE Aktiengesellschaft Procédé de détermination d'un partenaire d'une réaction et dispositif pour sa mise en oeuvre

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4409200A (en) * 1980-04-28 1983-10-11 Research Corporation Reverse transcriptase from human milk, method for its purification, and its use in the detection of breast cancer
JP2739615B2 (ja) * 1991-07-16 1998-04-15 富士写真フイルム株式会社 乾式免疫分析要素を用いた定量方法における検量線の設定方法
US6174729B1 (en) * 1995-01-10 2001-01-16 Aftab Alam Method, and kit for total protein assay

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0165551A2 (fr) * 1984-06-19 1985-12-27 BEHRINGWERKE Aktiengesellschaft Procédé de détermination d'un partenaire d'une réaction et dispositif pour sa mise en oeuvre
EP0165551A3 (fr) * 1984-06-19 1988-03-02 BEHRINGWERKE Aktiengesellschaft Procédé de détermination d'un partenaire d'une réaction et dispositif pour sa mise en oeuvre

Also Published As

Publication number Publication date
SE7610939L (sv) 1978-04-02
US4193981A (en) 1980-03-18
GB1549921A (en) 1979-08-08
JPS5379025A (en) 1978-07-13
DE2744028A1 (de) 1978-04-06

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Legal Events

Date Code Title Description
ST Notification of lapse