FR2252350A1 - Polynucleotide phosphorylase - extraction from E coli, fixation on sepharose, and use in preparing polynucleotides - Google Patents

Polynucleotide phosphorylase - extraction from E coli, fixation on sepharose, and use in preparing polynucleotides

Info

Publication number
FR2252350A1
FR2252350A1 FR7341662A FR7341662A FR2252350A1 FR 2252350 A1 FR2252350 A1 FR 2252350A1 FR 7341662 A FR7341662 A FR 7341662A FR 7341662 A FR7341662 A FR 7341662A FR 2252350 A1 FR2252350 A1 FR 2252350A1
Authority
FR
France
Prior art keywords
coli
diphosphate
sepharose
pnpase
fixation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR7341662A
Other languages
French (fr)
Other versions
FR2252350B1 (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Choay SA
Original Assignee
Choay SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Choay SA filed Critical Choay SA
Priority to FR7341662A priority Critical patent/FR2252350A1/en
Publication of FR2252350A1 publication Critical patent/FR2252350A1/en
Application granted granted Critical
Publication of FR2252350B1 publication Critical patent/FR2252350B1/fr
Granted legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1258—Polyribonucleotide nucleotidyltransferase (2.7.7.8), i.e. polynucleotide phosphorylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Polynucleotide phosphorylase (PNPase) with a mol. wt. of 200,000 + 10% composed of two sub-units with a mol. wt. of 95,000 + 5% free from nucleases, kinases, phosphatases and proteases is extracted from E.coli. The PNPase fixed on a sepharose support is used as a catalyst in the synthesis of homo- and co-polynucleotides. PNPase catalyses e.g. the polymsn. of inosine diphosphate, cytidine diphosphate, adenosine diphosphate, guanosine diphosphate, to form cpds. which are useful in the treatment of certain forms of leukemia and in the immunatory defence of the organisms.
FR7341662A 1973-11-22 1973-11-22 Polynucleotide phosphorylase - extraction from E coli, fixation on sepharose, and use in preparing polynucleotides Granted FR2252350A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
FR7341662A FR2252350A1 (en) 1973-11-22 1973-11-22 Polynucleotide phosphorylase - extraction from E coli, fixation on sepharose, and use in preparing polynucleotides

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR7341662A FR2252350A1 (en) 1973-11-22 1973-11-22 Polynucleotide phosphorylase - extraction from E coli, fixation on sepharose, and use in preparing polynucleotides

Publications (2)

Publication Number Publication Date
FR2252350A1 true FR2252350A1 (en) 1975-06-20
FR2252350B1 FR2252350B1 (en) 1977-03-11

Family

ID=9128134

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7341662A Granted FR2252350A1 (en) 1973-11-22 1973-11-22 Polynucleotide phosphorylase - extraction from E coli, fixation on sepharose, and use in preparing polynucleotides

Country Status (1)

Country Link
FR (1) FR2252350A1 (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2508059A1 (en) * 1981-06-23 1982-12-24 Centre Nat Rech Scient Plasmid incorporating polynucleotide phosphorylase gene - and a DNA fragment of a bacterial plasmid, and bacterial strains contg. the plasmid used to prepare phosphorylase polynucleotide
FR2622451A1 (en) * 1987-11-02 1989-05-05 Scras THERAPEUTIC COMPOSITIONS BASED ON POLYNUCLEOTIDES
US5756477A (en) * 1991-04-16 1998-05-26 Societe De Conseils De Recherches Et D'applications Scientifiques Treatment of aids with complex of poly (A). poly (U)

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2508059A1 (en) * 1981-06-23 1982-12-24 Centre Nat Rech Scient Plasmid incorporating polynucleotide phosphorylase gene - and a DNA fragment of a bacterial plasmid, and bacterial strains contg. the plasmid used to prepare phosphorylase polynucleotide
FR2622451A1 (en) * 1987-11-02 1989-05-05 Scras THERAPEUTIC COMPOSITIONS BASED ON POLYNUCLEOTIDES
FR2622586A1 (en) * 1987-11-02 1989-05-05 Scras NOVEL PROCESS FOR THE PREPARATION OF POLYNUCLEOTIDES AND THE PRODUCTS OBTAINED BY THIS METHOD
BE1001938A4 (en) * 1987-11-02 1990-04-17 Sod Conseils Rech Applic NOVEL PROCESS FOR THE PREPARATION OF THE POLYNUCLEOTIDES PRODUCED THUS OBTAINED AND PHARMACEUTICAL PREPARATIONS CONTAINING THE SAME.
US4927755A (en) * 1987-11-02 1990-05-22 Societe De Conseils De Recherches Et D'applicatios Scientifiques (S.C.R.A.S.) Process for preparing polynucleotides
GR1000715B (en) * 1987-11-02 1992-11-23 Scras Process for preparing polynucleotides
US5756477A (en) * 1991-04-16 1998-05-26 Societe De Conseils De Recherches Et D'applications Scientifiques Treatment of aids with complex of poly (A). poly (U)

Also Published As

Publication number Publication date
FR2252350B1 (en) 1977-03-11

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Legal Events

Date Code Title Description
ST Notification of lapse