FR2216992A1 - Low MW, purified collagenase prepn - by ion-exchange chromatography of enzyme from first-stage larvae of hypoderma spp. - Google Patents

Low MW, purified collagenase prepn - by ion-exchange chromatography of enzyme from first-stage larvae of hypoderma spp.

Info

Publication number
FR2216992A1
FR2216992A1 FR7304859A FR7304859A FR2216992A1 FR 2216992 A1 FR2216992 A1 FR 2216992A1 FR 7304859 A FR7304859 A FR 7304859A FR 7304859 A FR7304859 A FR 7304859A FR 2216992 A1 FR2216992 A1 FR 2216992A1
Authority
FR
France
Prior art keywords
enzyme
collagenase
prepn
ion
low
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR7304859A
Other languages
French (fr)
Other versions
FR2216992B1 (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bpifrance Financement SA
Original Assignee
Agence National de Valorisation de la Recherche ANVAR
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agence National de Valorisation de la Recherche ANVAR filed Critical Agence National de Valorisation de la Recherche ANVAR
Priority to FR7304859A priority Critical patent/FR2216992A1/en
Publication of FR2216992A1 publication Critical patent/FR2216992A1/en
Application granted granted Critical
Publication of FR2216992B1 publication Critical patent/FR2216992B1/fr
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6402—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals
    • C12N9/6405—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals not being snakes
    • C12N9/6416—Metalloendopeptidases (3.4.24)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Abstract

Pure collagenase, extracted from first-stage larvae of Hypoderma bovis or H.lineatum, which has a MW of 16,000 an IEP of pH 3.7, is inhibited by Hg2+, Ag+, Cu2+ and Sb3+ by diisopropyl fluorophosphate, p-chloromercuribenzoate and tris buffer, and is activated by Mn2+ and Ca2+. The cpd hypodermase, is used topically for treating burns, and as an antigen in hyperdermosis vaccines. It is of lower MW that bacterial collagenase (100,000) and that from fungi and synovial fluid (20,000). It is prepd by chromatography of crude collagenase on at least one ion exchange resin, and elution with pH 3.8 buffer. The pure enzyme is in the first fraction of eluate.
FR7304859A 1973-02-12 1973-02-12 Low MW, purified collagenase prepn - by ion-exchange chromatography of enzyme from first-stage larvae of hypoderma spp. Granted FR2216992A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
FR7304859A FR2216992A1 (en) 1973-02-12 1973-02-12 Low MW, purified collagenase prepn - by ion-exchange chromatography of enzyme from first-stage larvae of hypoderma spp.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR7304859A FR2216992A1 (en) 1973-02-12 1973-02-12 Low MW, purified collagenase prepn - by ion-exchange chromatography of enzyme from first-stage larvae of hypoderma spp.

Publications (2)

Publication Number Publication Date
FR2216992A1 true FR2216992A1 (en) 1974-09-06
FR2216992B1 FR2216992B1 (en) 1976-07-02

Family

ID=9114670

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7304859A Granted FR2216992A1 (en) 1973-02-12 1973-02-12 Low MW, purified collagenase prepn - by ion-exchange chromatography of enzyme from first-stage larvae of hypoderma spp.

Country Status (1)

Country Link
FR (1) FR2216992A1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5139944A (en) * 1985-08-10 1992-08-18 Biophram (Uk) Limited Collagen-specific enzyme with platelet aggregation inhibition properties

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1192937A (en) * 1966-07-08 1970-05-28 Agricultural Biolog Corp Process for Producing Collagenase

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1192937A (en) * 1966-07-08 1970-05-28 Agricultural Biolog Corp Process for Producing Collagenase

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5139944A (en) * 1985-08-10 1992-08-18 Biophram (Uk) Limited Collagen-specific enzyme with platelet aggregation inhibition properties

Also Published As

Publication number Publication date
FR2216992B1 (en) 1976-07-02

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Legal Events

Date Code Title Description
ST Notification of lapse