FR2213289A1 - Pure pig beta trypsin - obtd by chromatographic separation of commercial trypsin using sulphonated ion-exchange agents - Google Patents

Pure pig beta trypsin - obtd by chromatographic separation of commercial trypsin using sulphonated ion-exchange agents

Info

Publication number
FR2213289A1
FR2213289A1 FR7230127A FR7230127A FR2213289A1 FR 2213289 A1 FR2213289 A1 FR 2213289A1 FR 7230127 A FR7230127 A FR 7230127A FR 7230127 A FR7230127 A FR 7230127A FR 2213289 A1 FR2213289 A1 FR 2213289A1
Authority
FR
France
Prior art keywords
trypsin
buffer
obtd
commercial
pref
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR7230127A
Other languages
French (fr)
Other versions
FR2213289B1 (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Choay SA
Original Assignee
Choay SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Choay SA filed Critical Choay SA
Priority to FR7230127A priority Critical patent/FR2213289A1/en
Priority to US390340A priority patent/US3886043A/en
Priority to DK463973AA priority patent/DK135169B/en
Priority to DE19732342662 priority patent/DE2342662A1/en
Priority to JP48095627A priority patent/JPS4985287A/ja
Publication of FR2213289A1 publication Critical patent/FR2213289A1/en
Application granted granted Critical
Publication of FR2213289B1 publication Critical patent/FR2213289B1/fr
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6427—Chymotrypsins (3.4.21.1; 3.4.21.2); Trypsin (3.4.21.4)

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Pure beta-trypsin from pigs is prepd. from a commercial crystalline pig trypsin by chromatographic purification of a soln. of this material in a buffer with an elution coefficient equivalent to that of tris-(hydroxymethyl)aminomethane buffer with a resistance of >0.21x103 OMEGA, pref. between 0.23x103 and 0.35x103 OMEGA. Sulphonated ion exchange agents are used in the separation, esp. SE-Sephadex C-50 or Sp-Sephadex C-50 (RTM). Pref. buffer contains 0.05M tris buffer in Cl-form as well as 0.012-0.02M CaCl2 and 1mM benzamidine. Pref. pH is 7-8.2 at 1-4 degrees C. The prod. is more stable than customary bovine trypsin and uses previously unsuitable starting material.
FR7230127A 1972-08-24 1972-08-24 Pure pig beta trypsin - obtd by chromatographic separation of commercial trypsin using sulphonated ion-exchange agents Granted FR2213289A1 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
FR7230127A FR2213289A1 (en) 1972-08-24 1972-08-24 Pure pig beta trypsin - obtd by chromatographic separation of commercial trypsin using sulphonated ion-exchange agents
US390340A US3886043A (en) 1972-08-24 1973-08-22 Process for preparing pure porcine beta-trypsin
DK463973AA DK135169B (en) 1972-08-24 1973-08-23 Process for the preparation of a pure beta-trypsin derived from pigs.
DE19732342662 DE2342662A1 (en) 1972-08-24 1973-08-23 PROCESS FOR MANUFACTURING PURE PIG BETA-TRYPSINE AND BETA-TRYPSINE OBTAINED BY THIS PROCESS
JP48095627A JPS4985287A (en) 1972-08-24 1973-08-24

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR7230127A FR2213289A1 (en) 1972-08-24 1972-08-24 Pure pig beta trypsin - obtd by chromatographic separation of commercial trypsin using sulphonated ion-exchange agents

Publications (2)

Publication Number Publication Date
FR2213289A1 true FR2213289A1 (en) 1974-08-02
FR2213289B1 FR2213289B1 (en) 1975-03-28

Family

ID=9103500

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7230127A Granted FR2213289A1 (en) 1972-08-24 1972-08-24 Pure pig beta trypsin - obtd by chromatographic separation of commercial trypsin using sulphonated ion-exchange agents

Country Status (1)

Country Link
FR (1) FR2213289A1 (en)

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
NEANT *

Also Published As

Publication number Publication date
FR2213289B1 (en) 1975-03-28

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Legal Events

Date Code Title Description
ST Notification of lapse