ES8700319A1 - Pure chymopapain b: industrial process and therapeutic composition. - Google Patents

Pure chymopapain b: industrial process and therapeutic composition.

Info

Publication number
ES8700319A1
ES8700319A1 ES546394A ES546394A ES8700319A1 ES 8700319 A1 ES8700319 A1 ES 8700319A1 ES 546394 A ES546394 A ES 546394A ES 546394 A ES546394 A ES 546394A ES 8700319 A1 ES8700319 A1 ES 8700319A1
Authority
ES
Spain
Prior art keywords
chymopapain
cation exchange
crude
therapeutic composition
exchange resin
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES546394A
Other languages
Spanish (es)
Other versions
ES546394A0 (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of ES8700319A1 publication Critical patent/ES8700319A1/en
Publication of ES546394A0 publication Critical patent/ES546394A0/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/63—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/95—Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
    • G01N2333/964—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue
    • G01N2333/96425—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals
    • G01N2333/96427—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general
    • G01N2333/9643—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general with EC number
    • G01N2333/96466—Cysteine endopeptidases (3.4.22)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Botany (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

A process which permits the commercial scale isolation of large amounts of homogeneous populations of Chymopapain B or Chymopapain C, free from cross-contamination and other proteins and with low levels of pyrogens, from either papaya latex or crude chymopapain preparations. This process comprises mixing the papaya latex or crude chymopapain with increments of cation exchange resin to selectively absorb some but not all of the chymopapain onto the resin until the point is reached at which protease activity in the supernatant has decreased to a predetermined minimum level. The chymopapain is then eluted from the cation exchange resin. Therapeutic chymopapain-containing compositions are also disclosed, as is a standardized, reproducible enzyme assay for Chymopapain B and C.
ES546394A 1984-04-03 1985-08-23 Pure chymopapain b: industrial process and therapeutic composition. Expired ES8700319A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US59635684A 1984-04-03 1984-04-03

Publications (2)

Publication Number Publication Date
ES8700319A1 true ES8700319A1 (en) 1986-10-01
ES546394A0 ES546394A0 (en) 1986-10-01

Family

ID=24386988

Family Applications (2)

Application Number Title Priority Date Filing Date
ES535035A Expired ES8604303A1 (en) 1984-04-03 1984-08-09 Pure chymopapain b: industrial process and therapeutic composition.
ES546394A Expired ES8700319A1 (en) 1984-04-03 1985-08-23 Pure chymopapain b: industrial process and therapeutic composition.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES535035A Expired ES8604303A1 (en) 1984-04-03 1984-08-09 Pure chymopapain b: industrial process and therapeutic composition.

Country Status (8)

Country Link
EP (1) EP0175786A1 (en)
JP (1) JPS61502444A (en)
AU (1) AU4217885A (en)
ES (2) ES8604303A1 (en)
FI (1) FI854765A0 (en)
GB (1) GB2156821A (en)
NO (1) NO854823L (en)
WO (1) WO1985004417A1 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2193720B (en) * 1986-08-15 1990-09-19 Agricultural & Food Res Payaya proteinase b separation and uses
GB8909836D0 (en) * 1989-04-28 1989-06-14 Boots Co Plc Therapeutic agent
CN114054301B (en) * 2021-12-27 2022-06-24 南通世睿电力科技有限公司 Construction process of acrylic acid adhesive for plugging of power equipment under low-temperature condition

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3412150A (en) * 1965-01-15 1968-11-19 Navy Usa Nalpha-benzoyl arginine p-nitroanilide hydrochloride
US3558433A (en) * 1967-11-07 1971-01-26 Baxter Laboratories Inc Process for purification of chymopapain
US3623955A (en) * 1968-08-14 1971-11-30 Monsanto Co Purification and recovery of alkaline protease using cationic-exchange resin
FR2093139A5 (en) * 1970-06-03 1972-01-28 Roussel Uclaf
YU40433B (en) * 1975-02-20 1986-02-28 Lek Tovarna Farmacevtskih Process for obtaining pure, proteolytically active anzymes
US4086139A (en) * 1976-04-09 1978-04-25 Gb Fermentation Industries Inc. Differential inactivation of amylase in amylase-protease mixtures
US4390628A (en) * 1979-05-17 1983-06-28 De Forenede Bryggerier A/S Process for isolating Cu, Zn-superoxide dismutase from aqueous solutions containing said enzyme together with accompanying proteins
EP0065395B1 (en) * 1981-05-13 1988-08-24 BOOTS-FLINT, INC. (a Delaware corp.) Improved chymopapain and method for its production and use

Also Published As

Publication number Publication date
JPS61502444A (en) 1986-10-30
GB2156821A (en) 1985-10-16
ES535035A0 (en) 1986-01-16
GB8416926D0 (en) 1984-08-08
AU4217885A (en) 1985-11-01
FI854765A (en) 1985-12-02
FI854765A0 (en) 1985-12-02
ES546394A0 (en) 1986-10-01
EP0175786A1 (en) 1986-04-02
WO1985004417A1 (en) 1985-10-10
NO854823L (en) 1986-01-24
ES8604303A1 (en) 1986-01-16

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