FR2211469A1 - Enzyme purification, esp. ribonuclease, and (chymo) trypsin - by treatment with specific polyinhibitors and extraction or pptn of the complex formed - Google Patents

Enzyme purification, esp. ribonuclease, and (chymo) trypsin - by treatment with specific polyinhibitors and extraction or pptn of the complex formed

Info

Publication number
FR2211469A1
FR2211469A1 FR7245695A FR7245695A FR2211469A1 FR 2211469 A1 FR2211469 A1 FR 2211469A1 FR 7245695 A FR7245695 A FR 7245695A FR 7245695 A FR7245695 A FR 7245695A FR 2211469 A1 FR2211469 A1 FR 2211469A1
Authority
FR
France
Prior art keywords
polyinhibitors
pptn
extraction
treatment
complex
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
FR7245695A
Other languages
French (fr)
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Air Liquide SA
Original Assignee
Air Liquide SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Air Liquide SA filed Critical Air Liquide SA
Priority to FR7245695A priority Critical patent/FR2211469A1/en
Publication of FR2211469A1 publication Critical patent/FR2211469A1/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6427—Chymotrypsins (3.4.21.1; 3.4.21.2); Trypsin (3.4.21.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses

Abstract

Enzymes are isolated from natural mixtures by treatment with a complex-forming polyinhibitor which is initially soluble in aq. organic media. The complex is separated and broken down by change in pH or ionic strength and the polyinhibitor may be recycled. Suitable polyinhibitors have a polymeric chain, e.g. a polyoxyalkylene glycol which is reacted with a polyfunctional derivative, and then with an inhibitor for the specific enzyme, e.g. D-phenyl alanine or D-trytophan for use with chymotrypsin. Pptn. of the complexes is effected at low temp. in aq. alcoholic media keeping the dielectric constant at 75-82. If desired the complex formation and extraction may be effected in series to isolate a series of enzymes from the mixture.
FR7245695A 1972-12-21 1972-12-21 Enzyme purification, esp. ribonuclease, and (chymo) trypsin - by treatment with specific polyinhibitors and extraction or pptn of the complex formed Withdrawn FR2211469A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
FR7245695A FR2211469A1 (en) 1972-12-21 1972-12-21 Enzyme purification, esp. ribonuclease, and (chymo) trypsin - by treatment with specific polyinhibitors and extraction or pptn of the complex formed

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR7245695A FR2211469A1 (en) 1972-12-21 1972-12-21 Enzyme purification, esp. ribonuclease, and (chymo) trypsin - by treatment with specific polyinhibitors and extraction or pptn of the complex formed

Publications (1)

Publication Number Publication Date
FR2211469A1 true FR2211469A1 (en) 1974-07-19

Family

ID=9109110

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7245695A Withdrawn FR2211469A1 (en) 1972-12-21 1972-12-21 Enzyme purification, esp. ribonuclease, and (chymo) trypsin - by treatment with specific polyinhibitors and extraction or pptn of the complex formed

Country Status (1)

Country Link
FR (1) FR2211469A1 (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0046915A2 (en) * 1980-09-02 1982-03-10 Gambro Lundia AB A method for the recovery of a peptide-containing compound, and an apparatus for the realization of the method
US6541629B1 (en) 1999-09-10 2003-04-01 The Procter & Gamble Company Enzyme inhibitors
US6723788B1 (en) 1999-09-10 2004-04-20 The Procter & Gamble Company Enzyme inhibitors

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0046915A2 (en) * 1980-09-02 1982-03-10 Gambro Lundia AB A method for the recovery of a peptide-containing compound, and an apparatus for the realization of the method
EP0046915A3 (en) * 1980-09-02 1982-06-09 Gambro Lundia Ab A method for the recovery of a peptide-containing compound, and a means for the realization of the method
US6541629B1 (en) 1999-09-10 2003-04-01 The Procter & Gamble Company Enzyme inhibitors
US6723788B1 (en) 1999-09-10 2004-04-20 The Procter & Gamble Company Enzyme inhibitors

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