FI875642A0 - FARING EQUIPMENT FOR PLASMINOGENAKTIVATORER I PROKARYOTER. - Google Patents

FARING EQUIPMENT FOR PLASMINOGENAKTIVATORER I PROKARYOTER.

Info

Publication number
FI875642A0
FI875642A0 FI875642A FI875642A FI875642A0 FI 875642 A0 FI875642 A0 FI 875642A0 FI 875642 A FI875642 A FI 875642A FI 875642 A FI875642 A FI 875642A FI 875642 A0 FI875642 A0 FI 875642A0
Authority
FI
Finland
Prior art keywords
prokaryoter
plasminogenaktivatorer
replication
host cell
dissociated
Prior art date
Application number
FI875642A
Other languages
Finnish (fi)
Other versions
FI875642A (en
Inventor
Ralf Mattes
Original Assignee
Boehringer Mannheim Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim Gmbh filed Critical Boehringer Mannheim Gmbh
Publication of FI875642A publication Critical patent/FI875642A/en
Publication of FI875642A0 publication Critical patent/FI875642A0/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

In order to produce, in a procaryote cell, a plasminogen activator by expressing a c-DNA coding for this activator, which c-DNA is incorporated in a vector that is suitable for the host cell, a strictly controlled vector is used so that its replication cannot be dissociated, or can only be temporarily dissociated from the replication of the chromosome of the host cell. Its culture is carried out in conditions such that no laxity occurs in its control. Plasmids that are particularly suitable for carrying out this process are disclosed.
FI875642A 1986-04-21 1987-12-21 FARING EQUIPMENT FOR PLASMINOGENAKTIVATORER I PROKARYOTER. FI875642A0 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19863613401 DE3613401A1 (en) 1986-04-21 1986-04-21 METHOD FOR PRODUCING PLASMINOGEN ACTIVATORS IN PROKARYONTS
PCT/EP1987/000216 WO1987006611A1 (en) 1986-04-21 1987-04-21 Process for producing plasminogen-activators in procaryotes

Publications (2)

Publication Number Publication Date
FI875642A FI875642A (en) 1987-12-21
FI875642A0 true FI875642A0 (en) 1987-12-21

Family

ID=6299148

Family Applications (1)

Application Number Title Priority Date Filing Date
FI875642A FI875642A0 (en) 1986-04-21 1987-12-21 FARING EQUIPMENT FOR PLASMINOGENAKTIVATORER I PROKARYOTER.

Country Status (10)

Country Link
EP (1) EP0242835B1 (en)
JP (1) JPS63503035A (en)
AT (1) ATE85358T1 (en)
AU (1) AU601420B2 (en)
DE (2) DE3613401A1 (en)
FI (1) FI875642A0 (en)
HU (1) HUT46064A (en)
IL (1) IL82273A0 (en)
WO (1) WO1987006611A1 (en)
ZA (1) ZA872733B (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5266474A (en) * 1987-06-24 1993-11-30 Genentech, Inc. Balanced inducible transcription system
DE3832898A1 (en) * 1988-09-28 1990-04-12 Boehringer Mannheim Gmbh PRAEPARATE OF EXPRESSED PLASMINOGEN ACTIVATOR IN PROKARYONS
US6413720B1 (en) * 1999-01-11 2002-07-02 Signal Pharmaceuticals, Inc. Method for the affinity isolation of newly synthesized RNA

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL68366A (en) * 1982-04-15 1990-08-31 Genentech Inc Process for expressing a gene encoding a protein comprising the enzymatic portion of human urokinase in a microorganism or cell culture
IL68561A (en) * 1982-05-05 1991-01-31 Genentech Inc Preparation of polypeptide with human tissue plasminogen activator function,processes for making it,and dna and transformed cell intermediates thereof

Also Published As

Publication number Publication date
FI875642A (en) 1987-12-21
JPH0533030B2 (en) 1993-05-18
JPS63503035A (en) 1988-11-10
WO1987006611A1 (en) 1987-11-05
AU601420B2 (en) 1990-09-13
EP0242835B1 (en) 1993-02-03
EP0242835A1 (en) 1987-10-28
DE3613401A1 (en) 1987-12-17
AU7301287A (en) 1987-11-24
IL82273A0 (en) 1987-10-30
ZA872733B (en) 1987-10-06
DE3783934D1 (en) 1993-03-18
ATE85358T1 (en) 1993-02-15
HUT46064A (en) 1988-09-28

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Legal Events

Date Code Title Description
FD Application lapsed
MM Patent lapsed

Owner name: BOEHRINGER MANNHEIM GMBH