ZA872733B - A process for the production of plasminogen activators in prokaryonts - Google Patents

A process for the production of plasminogen activators in prokaryonts

Info

Publication number
ZA872733B
ZA872733B ZA872733A ZA872733A ZA872733B ZA 872733 B ZA872733 B ZA 872733B ZA 872733 A ZA872733 A ZA 872733A ZA 872733 A ZA872733 A ZA 872733A ZA 872733 B ZA872733 B ZA 872733B
Authority
ZA
South Africa
Prior art keywords
prokaryonts
production
plasminogen activators
replication
host cell
Prior art date
Application number
ZA872733A
Inventor
Mattes Ralf
Original Assignee
Boehringer Mannheim Gmbh.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim Gmbh. filed Critical Boehringer Mannheim Gmbh.
Publication of ZA872733B publication Critical patent/ZA872733B/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

In order to produce, in a procaryote cell, a plasminogen activator by expressing a c-DNA coding for this activator, which c-DNA is incorporated in a vector that is suitable for the host cell, a strictly controlled vector is used so that its replication cannot be dissociated, or can only be temporarily dissociated from the replication of the chromosome of the host cell. Its culture is carried out in conditions such that no laxity occurs in its control. Plasmids that are particularly suitable for carrying out this process are disclosed.
ZA872733A 1986-04-21 1987-04-16 A process for the production of plasminogen activators in prokaryonts ZA872733B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19863613401 DE3613401A1 (en) 1986-04-21 1986-04-21 METHOD FOR PRODUCING PLASMINOGEN ACTIVATORS IN PROKARYONTS

Publications (1)

Publication Number Publication Date
ZA872733B true ZA872733B (en) 1987-10-06

Family

ID=6299148

Family Applications (1)

Application Number Title Priority Date Filing Date
ZA872733A ZA872733B (en) 1986-04-21 1987-04-16 A process for the production of plasminogen activators in prokaryonts

Country Status (10)

Country Link
EP (1) EP0242835B1 (en)
JP (1) JPS63503035A (en)
AT (1) ATE85358T1 (en)
AU (1) AU601420B2 (en)
DE (2) DE3613401A1 (en)
FI (1) FI875642A0 (en)
HU (1) HUT46064A (en)
IL (1) IL82273A0 (en)
WO (1) WO1987006611A1 (en)
ZA (1) ZA872733B (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5266474A (en) * 1987-06-24 1993-11-30 Genentech, Inc. Balanced inducible transcription system
DE3832898A1 (en) * 1988-09-28 1990-04-12 Boehringer Mannheim Gmbh PRAEPARATE OF EXPRESSED PLASMINOGEN ACTIVATOR IN PROKARYONS
US6413720B1 (en) * 1999-01-11 2002-07-02 Signal Pharmaceuticals, Inc. Method for the affinity isolation of newly synthesized RNA

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI88932C (en) * 1982-04-15 1993-07-26 Genentech Inc Preparation of functional human urokinase protein
IL68561A (en) * 1982-05-05 1991-01-31 Genentech Inc Preparation of polypeptide with human tissue plasminogen activator function,processes for making it,and dna and transformed cell intermediates thereof

Also Published As

Publication number Publication date
JPS63503035A (en) 1988-11-10
HUT46064A (en) 1988-09-28
IL82273A0 (en) 1987-10-30
DE3613401A1 (en) 1987-12-17
JPH0533030B2 (en) 1993-05-18
EP0242835A1 (en) 1987-10-28
ATE85358T1 (en) 1993-02-15
DE3783934D1 (en) 1993-03-18
FI875642A (en) 1987-12-21
AU601420B2 (en) 1990-09-13
EP0242835B1 (en) 1993-02-03
FI875642A0 (en) 1987-12-21
WO1987006611A1 (en) 1987-11-05
AU7301287A (en) 1987-11-24

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