FI873153A - Process for Preparation of Human Antithrombin III (ATIII) in a Mammalian Cell Culture and Useful Vectors and Mammalian Host Cells - Google Patents

Process for Preparation of Human Antithrombin III (ATIII) in a Mammalian Cell Culture and Useful Vectors and Mammalian Host Cells Download PDF

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Publication number
FI873153A
FI873153A FI873153A FI873153A FI873153A FI 873153 A FI873153 A FI 873153A FI 873153 A FI873153 A FI 873153A FI 873153 A FI873153 A FI 873153A FI 873153 A FI873153 A FI 873153A
Authority
FI
Finland
Prior art keywords
atiii
antithrombin iii
preparation
cell culture
host cells
Prior art date
Application number
FI873153A
Other languages
Finnish (fi)
Swedish (sv)
Other versions
FI94425B (en
FI873153A0 (en
FI94425C (en
Inventor
Gerd Zettmeissl
Michael Broeker
Hermann Ragg
Original Assignee
Behringwerke Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Behringwerke Ag filed Critical Behringwerke Ag
Publication of FI873153A0 publication Critical patent/FI873153A0/en
Publication of FI873153A publication Critical patent/FI873153A/en
Publication of FI94425B publication Critical patent/FI94425B/en
Application granted granted Critical
Publication of FI94425C publication Critical patent/FI94425C/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
    • C07K14/8121—Serpins
    • C07K14/8128—Antithrombin III
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/108—Plasmid DNA episomal vectors

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

Human antithrombin III (ATIII) which has progressive inhibitory activity and is stimulated by heparin is advantageously obtained by expression after cotransfection of mammalian cells whose dihydrofolate reductase (DHFR) activity is deficient (dhfr<->) with a) vectors which contain the ATIII cDNA and b) vectors which harbour the DHFR gene, especially after gene amplification with methotrexate.
FI873153A 1986-07-19 1987-07-16 Method for the production of human antithrombin III (ATIII) in mammalian cell culture and suitable vectors and mammalian host cells FI94425C (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE3624453 1986-07-19
DE19863624453 DE3624453A1 (en) 1986-07-19 1986-07-19 METHOD FOR THE PRODUCTION OF HUMAN ANTITHROMBIN III (ATIII), VECTORS AND Host CELLS SUITABLE FOR THEM, BIOLOGICALLY ACTIVE ATIII, AND THE MEDICINAL PRODUCT CONTAINING THEM

Publications (4)

Publication Number Publication Date
FI873153A0 FI873153A0 (en) 1987-07-16
FI873153A true FI873153A (en) 1988-01-20
FI94425B FI94425B (en) 1995-05-31
FI94425C FI94425C (en) 1995-09-11

Family

ID=6305549

Family Applications (1)

Application Number Title Priority Date Filing Date
FI873153A FI94425C (en) 1986-07-19 1987-07-16 Method for the production of human antithrombin III (ATIII) in mammalian cell culture and suitable vectors and mammalian host cells

Country Status (11)

Country Link
EP (1) EP0256302B1 (en)
JP (2) JP2721158B2 (en)
AT (1) ATE85348T1 (en)
AU (1) AU610830B2 (en)
CA (1) CA1305932C (en)
DE (2) DE3624453A1 (en)
DK (1) DK174993B1 (en)
ES (1) ES2053476T3 (en)
FI (1) FI94425C (en)
GR (1) GR3007700T3 (en)
PT (1) PT85346B (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH03215430A (en) * 1990-01-19 1991-09-20 Kita Kiyoshi Anti-coagulant for articular cavity
DE3901917A1 (en) * 1989-01-24 1990-07-26 Behringwerke Ag MUTANTS OF HUMAN ANTITHROMBIN III
MXPA04005191A (en) * 2001-11-28 2005-04-29 Sandoz Ag Method for producing a recombinant polypeptide.

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS597693B2 (en) * 1978-01-07 1984-02-20 株式会社ミドリ十字 Antithrombin preparation and its manufacturing method
JPS5846100A (en) * 1981-09-11 1983-03-17 Agency Of Ind Science & Technol Plasmid vector having three kinds of marker and its preparation
GB2116183B (en) * 1982-03-03 1985-06-05 Genentech Inc Human antithrombin iii dna sequences therefore expression vehicles and cloning vectors containing such sequences and cell cultures transformed thereby a process for expressing human antithrombin iii and pharmaceutical compositions comprising it
DE3382068D1 (en) * 1982-03-15 1991-01-31 Univ Columbia METHOD FOR INTRODUCING CLONED AMPLIFICABLE GENES IN EUKARYOTIC CELLS AND FOR THE PRODUCTION OF PROTEIN PRODUCTS.
IL73854A0 (en) * 1983-12-27 1985-03-31 Genetics Inst Vectors containing accessory dna for transformation of eucaryotic cells
IL71691A (en) * 1984-04-27 1991-04-15 Yeda Res & Dev Production of interferon-ypsilon
US4656254A (en) * 1985-12-02 1987-04-07 Miles Laboratories, Inc. Method of preparing alpha-1-proteinase inhibitor and antithrombin III

Also Published As

Publication number Publication date
FI94425B (en) 1995-05-31
ES2053476T3 (en) 1994-08-01
ATE85348T1 (en) 1993-02-15
EP0256302A2 (en) 1988-02-24
AU7577387A (en) 1988-03-03
CA1305932C (en) 1992-08-04
DE3624453A1 (en) 1988-01-28
FI873153A0 (en) 1987-07-16
AU610830B2 (en) 1991-05-30
JP2798659B2 (en) 1998-09-17
JPH09117293A (en) 1997-05-06
EP0256302A3 (en) 1989-11-02
JP2721158B2 (en) 1998-03-04
DK375187A (en) 1988-01-20
PT85346B (en) 1990-06-29
DK174993B1 (en) 2004-04-13
PT85346A (en) 1987-08-01
JPS6344898A (en) 1988-02-25
GR3007700T3 (en) 1993-08-31
EP0256302B1 (en) 1993-02-03
DK375187D0 (en) 1987-07-17
FI94425C (en) 1995-09-11
DE3783968D1 (en) 1993-03-18

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BB Publication of examined application
FG Patent granted

Owner name: BEHRINGWERKE AKTIENGESELLSCHAFT

MA Patent expired