FI57024C - Foerfarande foer kvantitativ bestaemning av ammoniak i blodet - Google Patents
Foerfarande foer kvantitativ bestaemning av ammoniak i blodet Download PDFInfo
- Publication number
- FI57024C FI57024C FI1705/74A FI170574A FI57024C FI 57024 C FI57024 C FI 57024C FI 1705/74 A FI1705/74 A FI 1705/74A FI 170574 A FI170574 A FI 170574A FI 57024 C FI57024 C FI 57024C
- Authority
- FI
- Finland
- Prior art keywords
- buffer
- nadph
- coenzyme
- ketoglutarate
- adp
- Prior art date
Links
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 title claims description 25
- 229910021529 ammonia Inorganic materials 0.000 title claims description 12
- 210000004369 blood Anatomy 0.000 title claims description 9
- 239000008280 blood Substances 0.000 title claims description 9
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 claims description 23
- 238000000034 method Methods 0.000 claims description 21
- 101000950981 Bacillus subtilis (strain 168) Catabolic NAD-specific glutamate dehydrogenase RocG Proteins 0.000 claims description 13
- 102000016901 Glutamate dehydrogenase Human genes 0.000 claims description 13
- 239000000872 buffer Substances 0.000 claims description 12
- 239000003153 chemical reaction reagent Substances 0.000 claims description 10
- KPGXRSRHYNQIFN-UHFFFAOYSA-N 2-oxoglutaric acid Chemical compound OC(=O)CCC(=O)C(O)=O KPGXRSRHYNQIFN-UHFFFAOYSA-N 0.000 claims description 9
- 239000005515 coenzyme Substances 0.000 claims description 7
- GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 claims description 4
- 229960004418 trolamine Drugs 0.000 claims description 4
- 229920002307 Dextran Polymers 0.000 claims description 3
- 102000007562 Serum Albumin Human genes 0.000 claims description 3
- 108010071390 Serum Albumin Proteins 0.000 claims description 3
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims description 3
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 claims description 2
- 239000007983 Tris buffer Substances 0.000 claims description 2
- 239000003513 alkali Substances 0.000 claims description 2
- 150000001540 azides Chemical class 0.000 claims description 2
- 229930195712 glutamate Natural products 0.000 claims description 2
- ACFIXJIJDZMPPO-NNYOXOHSSA-N NADPH Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](OP(O)(O)=O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 ACFIXJIJDZMPPO-NNYOXOHSSA-N 0.000 claims 3
- 150000001875 compounds Chemical class 0.000 claims 1
- 210000005036 nerve Anatomy 0.000 claims 1
- 238000012207 quantitative assay Methods 0.000 claims 1
- XJLXINKUBYWONI-DQQFMEOOSA-N [[(2r,3r,4r,5r)-5-(6-aminopurin-9-yl)-3-hydroxy-4-phosphonooxyoxolan-2-yl]methoxy-hydroxyphosphoryl] [(2s,3r,4s,5s)-5-(3-carbamoylpyridin-1-ium-1-yl)-3,4-dihydroxyoxolan-2-yl]methyl phosphate Chemical compound NC(=O)C1=CC=C[N+]([C@@H]2[C@H]([C@@H](O)[C@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](OP(O)(O)=O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 XJLXINKUBYWONI-DQQFMEOOSA-N 0.000 description 15
- 238000003556 assay Methods 0.000 description 11
- 230000008033 biological extinction Effects 0.000 description 10
- XTWYTFMLZFPYCI-KQYNXXCUSA-N 5'-adenylphosphoric acid Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O XTWYTFMLZFPYCI-KQYNXXCUSA-N 0.000 description 8
- XTWYTFMLZFPYCI-UHFFFAOYSA-N Adenosine diphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(O)=O)C(O)C1O XTWYTFMLZFPYCI-UHFFFAOYSA-N 0.000 description 8
- 239000000203 mixture Substances 0.000 description 7
- 238000006243 chemical reaction Methods 0.000 description 6
- BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 description 6
- 102000004169 proteins and genes Human genes 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 6
- 239000011541 reaction mixture Substances 0.000 description 5
- 102000004190 Enzymes Human genes 0.000 description 4
- 108090000790 Enzymes Proteins 0.000 description 4
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 4
- 238000007086 side reaction Methods 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 3
- 238000011533 pre-incubation Methods 0.000 description 3
- 239000003381 stabilizer Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- KPGXRSRHYNQIFN-UHFFFAOYSA-L 2-oxoglutarate(2-) Chemical compound [O-]C(=O)CCC(=O)C([O-])=O KPGXRSRHYNQIFN-UHFFFAOYSA-L 0.000 description 2
- GJMPSRSMBJLKKB-UHFFFAOYSA-N 3-methylphenylacetic acid Chemical compound CC1=CC=CC(CC(O)=O)=C1 GJMPSRSMBJLKKB-UHFFFAOYSA-N 0.000 description 2
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
- 108010046334 Urease Proteins 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 239000008363 phosphate buffer Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 229940117957 triethanolamine hydrochloride Drugs 0.000 description 2
- 102000009027 Albumins Human genes 0.000 description 1
- 108010088751 Albumins Proteins 0.000 description 1
- 206010010071 Coma Diseases 0.000 description 1
- 206010052770 Coma states Diseases 0.000 description 1
- 108010020056 Hydrogenase Proteins 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- DFPAKSUCGFBDDF-ZQBYOMGUSA-N [14c]-nicotinamide Chemical compound N[14C](=O)C1=CC=CN=C1 DFPAKSUCGFBDDF-ZQBYOMGUSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 235000019270 ammonium chloride Nutrition 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 239000003918 blood extract Substances 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000012937 correction Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000001627 detrimental effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000003748 differential diagnosis Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- 230000002440 hepatic effect Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000011005 laboratory method Methods 0.000 description 1
- 208000019423 liver disease Diseases 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 229950006238 nadide Drugs 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- 235000017557 sodium bicarbonate Nutrition 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
- C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/58—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving urea or urease
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE2329174 | 1973-06-07 | ||
| DE2329174A DE2329174C2 (de) | 1973-06-07 | 1973-06-07 | Verfahren zur quantitativen Bestimmung von Blutammoniak |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| FI170574A7 FI170574A7 (cs) | 1974-12-08 |
| FI57024B FI57024B (fi) | 1980-01-31 |
| FI57024C true FI57024C (fi) | 1980-05-12 |
Family
ID=5883399
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FI1705/74A FI57024C (fi) | 1973-06-07 | 1974-06-04 | Foerfarande foer kvantitativ bestaemning av ammoniak i blodet |
Country Status (15)
| Country | Link |
|---|---|
| US (1) | US3929581A (cs) |
| JP (1) | JPS5721995B2 (cs) |
| AR (1) | AR199957A1 (cs) |
| AT (1) | AT330367B (cs) |
| CA (1) | CA1016050A (cs) |
| CH (1) | CH600339A5 (cs) |
| DD (1) | DD112525A5 (cs) |
| DE (1) | DE2329174C2 (cs) |
| FI (1) | FI57024C (cs) |
| FR (1) | FR2232760B1 (cs) |
| GB (1) | GB1417538A (cs) |
| HU (1) | HU168366B (cs) |
| IT (1) | IT1017557B (cs) |
| NL (1) | NL179490C (cs) |
| SE (1) | SE417839B (cs) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL7603588A (nl) * | 1975-04-21 | 1976-10-25 | Hoffmann La Roche | Gestabiliseerde coenzymoplossing. |
| DE2612726C3 (de) * | 1976-03-25 | 1979-03-15 | Boehringer Mannheim Gmbh, 6800 Mannheim | Stabilisierte Urease |
| CA1187388A (en) * | 1978-09-20 | 1985-05-21 | American Monitor Corporation | Stabilization of working reagent solutions containing nadh, nadph, and/or enzymes, and the use of such stabilized reagents in enzymes or substrate assays |
| JPS6041500A (ja) * | 1983-08-12 | 1985-03-05 | Yamasa Shoyu Co Ltd | アンモニアの定量法 |
| US4935346A (en) | 1986-08-13 | 1990-06-19 | Lifescan, Inc. | Minimum procedure system for the determination of analytes |
| US5141853A (en) * | 1988-07-22 | 1992-08-25 | Abbott Laboratories | Determination of ammonia levels in a sample |
| ES2084628T3 (es) * | 1989-08-28 | 1996-05-16 | Hoffmann La Roche | Metodo enzimatico para determinar las concentraciones del producto analizado. |
| JP3034969B2 (ja) * | 1991-03-01 | 2000-04-17 | 旭化成工業株式会社 | アンモニア、α−アミノ酸類またはα−ケト酸の高感度定量法および高感度定量用組成物 |
| DE4231266A1 (de) * | 1992-09-18 | 1994-03-24 | Boehringer Mannheim Gmbh | Dextran modifizierte Glutamat-Dehydrogenase |
| US6458326B1 (en) | 1999-11-24 | 2002-10-01 | Home Diagnostics, Inc. | Protective test strip platform |
| US6525330B2 (en) | 2001-02-28 | 2003-02-25 | Home Diagnostics, Inc. | Method of strip insertion detection |
| US6562625B2 (en) | 2001-02-28 | 2003-05-13 | Home Diagnostics, Inc. | Distinguishing test types through spectral analysis |
| US6541266B2 (en) | 2001-02-28 | 2003-04-01 | Home Diagnostics, Inc. | Method for determining concentration of an analyte in a test strip |
| JP5218949B2 (ja) | 2006-10-18 | 2013-06-26 | 国立大学法人名古屋大学 | Ｄ−セリンデヒドラターゼ及びその利用 |
| WO2016083399A1 (en) * | 2014-11-24 | 2016-06-02 | Forschungsgesellschaft Für Arbeitsphysiologie Und Arbeitsschutz E. V. | Alpha-ketoglutarate in combination with glutamate dehydrogenase for treating hyperammonemia |
| CN104374906B (zh) * | 2014-11-28 | 2016-02-17 | 山东博科生物产业有限公司 | 一种血清氨检测试剂 |
| BR112021014997A2 (pt) * | 2019-01-31 | 2021-10-05 | F. Hoffmann-La Roche Ag | Kit de ensaio para determinar quantitativamente a concentração de amônia em uma amostra líquida aquosa, método para fornecer um kit de ensaio, uso de um kit de ensaio, kit de ensaio, mistura e dispositivo |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3527674A (en) * | 1966-06-30 | 1970-09-08 | Calbiochem | Reagent material and method for urea assay |
-
1973
- 1973-06-07 DE DE2329174A patent/DE2329174C2/de not_active Expired
-
1974
- 1974-03-22 AT AT239274A patent/AT330367B/de not_active IP Right Cessation
- 1974-04-18 IT IT21615/74A patent/IT1017557B/it active
- 1974-04-29 AR AR253512A patent/AR199957A1/es active
- 1974-05-01 NL NLAANVRAGE7405809,A patent/NL179490C/xx not_active IP Right Cessation
- 1974-05-17 US US471064A patent/US3929581A/en not_active Expired - Lifetime
- 1974-05-27 CA CA200,847A patent/CA1016050A/en not_active Expired
- 1974-06-04 JP JP6331974A patent/JPS5721995B2/ja not_active Expired
- 1974-06-04 FI FI1705/74A patent/FI57024C/fi active
- 1974-06-05 GB GB2487674A patent/GB1417538A/en not_active Expired
- 1974-06-06 SE SE7407447A patent/SE417839B/xx not_active IP Right Cessation
- 1974-06-06 HU HUBO1502A patent/HU168366B/hu unknown
- 1974-06-07 CH CH785074A patent/CH600339A5/xx not_active IP Right Cessation
- 1974-06-07 FR FR7419841A patent/FR2232760B1/fr not_active Expired
- 1974-06-07 DD DD179020A patent/DD112525A5/xx unknown
Also Published As
| Publication number | Publication date |
|---|---|
| DE2329174C2 (de) | 1975-07-24 |
| NL7405809A (cs) | 1974-12-10 |
| NL179490B (nl) | 1986-04-16 |
| AR199957A1 (es) | 1974-10-08 |
| NL179490C (nl) | 1986-09-16 |
| SE7407447L (cs) | 1974-12-09 |
| FI57024B (fi) | 1980-01-31 |
| DE2329174B1 (de) | 1974-11-28 |
| US3929581A (en) | 1975-12-30 |
| ATA239274A (de) | 1975-09-15 |
| DD112525A5 (cs) | 1975-04-12 |
| FI170574A7 (cs) | 1974-12-08 |
| CH600339A5 (cs) | 1978-06-15 |
| SE417839B (sv) | 1981-04-13 |
| FR2232760B1 (cs) | 1978-01-13 |
| JPS5023699A (cs) | 1975-03-13 |
| HU168366B (cs) | 1976-04-28 |
| FR2232760A1 (cs) | 1975-01-03 |
| AT330367B (de) | 1976-06-25 |
| JPS5721995B2 (cs) | 1982-05-11 |
| CA1016050A (en) | 1977-08-23 |
| IT1017557B (it) | 1977-08-10 |
| GB1417538A (en) | 1975-12-10 |
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