FI3671200T3 - Ce-western-sovelluksia vasta-aineen kehittämiseksi - Google Patents

Ce-western-sovelluksia vasta-aineen kehittämiseksi Download PDF

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Publication number
FI3671200T3
FI3671200T3 FIEP19218272.3T FI19218272T FI3671200T3 FI 3671200 T3 FI3671200 T3 FI 3671200T3 FI 19218272 T FI19218272 T FI 19218272T FI 3671200 T3 FI3671200 T3 FI 3671200T3
Authority
FI
Finland
Prior art keywords
antibody
primary antibodies
sample
antibodies
detecting
Prior art date
Application number
FIEP19218272.3T
Other languages
English (en)
Finnish (fi)
Inventor
Nisha Palackal
Kun Lu
Gangadhar Dhulipala
Erica Pyles
Original Assignee
Regeneron Pharma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Regeneron Pharma filed Critical Regeneron Pharma
Application granted granted Critical
Publication of FI3671200T3 publication Critical patent/FI3671200T3/fi

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G01N27/44726—Arrangements for investigating the separated zones, e.g. localising zones by optical means using specific dyes, markers or binding molecules
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44743—Introducing samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44795—Isoelectric focusing

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
FIEP19218272.3T 2018-12-19 2019-12-19 Ce-western-sovelluksia vasta-aineen kehittämiseksi FI3671200T3 (fi)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201862781790P 2018-12-19 2018-12-19

Publications (1)

Publication Number Publication Date
FI3671200T3 true FI3671200T3 (fi) 2023-08-28

Family

ID=69410996

Family Applications (1)

Application Number Title Priority Date Filing Date
FIEP19218272.3T FI3671200T3 (fi) 2018-12-19 2019-12-19 Ce-western-sovelluksia vasta-aineen kehittämiseksi

Country Status (9)

Country Link
US (2) US11782023B2 (enExample)
EP (2) EP3671200B1 (enExample)
JP (1) JP2020101545A (enExample)
CA (1) CA3065460A1 (enExample)
DK (1) DK3671200T3 (enExample)
ES (1) ES2955171T3 (enExample)
FI (1) FI3671200T3 (enExample)
HU (1) HUE062818T2 (enExample)
PL (1) PL3671200T3 (enExample)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112021023206A2 (pt) * 2019-05-21 2022-01-04 Regeneron Pharma Métodos para identificar e quantificar proteína de célula hospedeira
EP4493921A1 (en) * 2022-03-18 2025-01-22 Regeneron Pharmaceuticals, Inc. Methods and systems for analyzing polypeptide variants

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5002582A (en) 1982-09-29 1991-03-26 Bio-Metric Systems, Inc. Preparation of polymeric surfaces via covalently attaching polymers
US6087188A (en) 1992-11-13 2000-07-11 Alk A/S Two-site immunoassay for an antibody with chemiluminescent label and biotin bound ligand
JPH09507571A (ja) 1993-12-23 1997-07-29 トロピックス・インコーポレーテッド 化学発光エネルギー移動検定
US5866683A (en) 1995-03-31 1999-02-02 Laboratory Of Molecular Biophotonics Isoelectric point markers for isoelectric focusing with fluorescence detection
US6165800A (en) 1997-05-30 2000-12-26 Bayer Corporation Chemiluminescent energy transfer conjugates and their uses as labels in binding assays
US6126870A (en) 1997-09-12 2000-10-03 Lumigen, Inc. Chemiluminescent labeling compounds
US6254634B1 (en) 1998-06-10 2001-07-03 Surmodics, Inc. Coating compositions
WO2000009626A1 (en) 1998-08-14 2000-02-24 Dainichiseika Color & Chemicals Mfg. Co., Ltd. Chemiluminescent reagents and chemiluminescence analysis methods with the use of the same
ATE556149T1 (de) 1999-02-23 2012-05-15 Caliper Life Sciences Inc Manipulation von mikropartikeln in mikrofluidischen systemen
JP2001249079A (ja) 1999-12-28 2001-09-14 Fujirebio Inc 発光量調節剤を用いた化学発光測定方法及び酵素免疫測定方法
JP5073491B2 (ja) * 2004-07-19 2012-11-14 プロテインシンプル 分析対象物の検出のための方法
US8945361B2 (en) 2005-09-20 2015-02-03 ProteinSimple Electrophoresis standards, methods and kits
US20090023156A1 (en) * 2007-07-20 2009-01-22 Voss Karl O Methods and reagents for quantifying analytes
LT2975051T (lt) * 2009-06-26 2021-07-12 Regeneron Pharmaceuticals, Inc. Lengvai išskiriami bispecifiniai antikūnai su natyvios formos imunoglobulinu
US9766206B2 (en) * 2013-09-27 2017-09-19 ProteinSimple Apparatus, systems, and methods for capillary electrophoresis
WO2016183222A1 (en) * 2015-05-12 2016-11-17 Regeneron Pharmaceuticals, Inc. Multimeric protein purity determination

Also Published As

Publication number Publication date
HUE062818T2 (hu) 2023-12-28
PL3671200T3 (pl) 2023-10-09
DK3671200T3 (da) 2023-08-21
EP3671200A1 (en) 2020-06-24
EP3671200B1 (en) 2023-06-21
EP4253950A3 (en) 2024-01-17
CA3065460A1 (en) 2020-06-19
US20200200707A1 (en) 2020-06-25
ES2955171T3 (es) 2023-11-29
EP4253950A2 (en) 2023-10-04
US11782023B2 (en) 2023-10-10
US20230417701A1 (en) 2023-12-28
JP2020101545A (ja) 2020-07-02

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