FI124531B - Menetelmä radioaktiivisuuden mittaamiseksi - Google Patents

Menetelmä radioaktiivisuuden mittaamiseksi Download PDF

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Publication number
FI124531B
FI124531B FI20125303A FI20125303A FI124531B FI 124531 B FI124531 B FI 124531B FI 20125303 A FI20125303 A FI 20125303A FI 20125303 A FI20125303 A FI 20125303A FI 124531 B FI124531 B FI 124531B
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Prior art keywords
matrix
molecules
radioactivity
sample
silver
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FI20125303A
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English (en)
Swedish (sv)
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FI20125303A (fi
Inventor
Marja Tiirola
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Marja Tiirola
Maasilta Ilari
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Priority to FI20125303A priority Critical patent/FI124531B/fi
Priority to US14/382,512 priority patent/US9869672B2/en
Priority to EP13764018.1A priority patent/EP2839274B1/en
Priority to PCT/FI2013/050308 priority patent/WO2013140040A1/en
Publication of FI20125303A publication Critical patent/FI20125303A/fi
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/403—Cells and electrode assemblies
    • G01N27/414—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/403—Cells and electrode assemblies
    • G01N27/414—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS
    • G01N27/4145—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS specially adapted for biomolecules, e.g. gate electrode with immobilised receptors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/534—Production of labelled immunochemicals with radioactive label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/60—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances involving radioactive labelled substances
    • G—PHYSICS
    • G03—PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
    • G03C—PHOTOSENSITIVE MATERIALS FOR PHOTOGRAPHIC PURPOSES; PHOTOGRAPHIC PROCESSES, e.g. CINE, X-RAY, COLOUR, STEREO-PHOTOGRAPHIC PROCESSES; AUXILIARY PROCESSES IN PHOTOGRAPHY
    • G03C5/00—Photographic processes or agents therefor; Regeneration of such processing agents
    • G03C5/26—Processes using silver-salt-containing photosensitive materials or agents therefor

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Microbiology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Microelectronics & Electronic Packaging (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Biophysics (AREA)
  • Sustainable Development (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Testing Or Measuring Of Semiconductors Or The Like (AREA)

Claims (10)

1. Menetelmä radioaktiivisuuden määrittämiseksi ioniherkällä kanavatransistorimatriisilla, joka menetelmä käsittää vaiheet: a) kiinnitetään näytteen molekyylit reaktiolokeroon mainitussa matriisissa; b) jos mainittu näyte ei sisällä radioaktiivisuutta, saatetaan mainittu matriisi kontaktiin radioaktiivisen molekyylin tai molekyylien kanssa, jolla radioaktiivisella molekyylillä tai molekyyleillä on spesifiset sitoutumisominaisuudet; c) inkuboidaan matriisia elektroniherkän hopeahalidimateriaalin kanssa; ja d) inkuboidaan matriisia kehitysliuoksella, joka pelkistää radioaktiivisuudelle altistuneet hopeahalidit alkuainehopeaksi ja samanaikaisesti mitataan pH:ta kussakin erillisessä reaktiolokerossa, jolloin pH:n aleneminen osoittaa radioaktiivisuuden läsnäolon reaktiolokerossa.
2. Patenttivaatimuksen 1 mukainen menetelmä, jolloin mainittu näyte käsittää nukleiinihappomolekyylejä, jotka kiinnitetään matriisiin vaiheessa a).
3. Patenttivaatimuksen 2 mukainen menetelmä, jolloin mainittu radioaktiivinen molekyyli on radioaktiivisesti leimattu nukleiinihappo, proteiini tai vasta-aine.
4. Patenttivaatimuksen 2 tai 3 mukainen menetelmä, jolloin menetelmä käsittää lisävaiheen, jossa sekvensoidaan nukleiinihappomolekyylit, jotka on kiinnitetty matriisiin vaiheessa a) siten, että sekvensointi suoritetaan ennen kuin matriisi on kontaktissa mainitun radioaktiivisen molekyylin kanssa vaiheessa b).
5. Patenttivaatimuksen 1 mukainen menetelmä, jolloin mainittu näyte käsittää peptidejä tai polypeptidejä, jotka on kiinnitetty matriisiin vaiheessa a).
^ 6. Patenttivaatimuksen 5 mukainen menetelmä, jolloin mainittu radioaktiivinen molekyyli on o ^ radioaktiivisesti leimattu vasta-aine. i c\j o c\i
7. Patenttivaatimuksen 5 tai 6 mukainen menetelmä, jolloin mainitut reaktiolokerot pinnoitetaan ir vasta-aineilla ennen näytteen peptidien tai polypeptidien kiinnittämistä mainitulle matriisille. CL CO o co
8. Ioniherkän kanavatransistorimatriisin käsittävän laitteen käyttö jonkin patenttivaatimuksista 1-7 cvj ^ mukaisen menetelmän suorittamiseksi, jolloin mainittu laite on ohjelmoitu suorittamaan mainittu ^ menetelmä.
9. Tuotepakkaus patenttivaatimuksen 1, 4 tai 6 mukaisen menetelmän suorittamiseksi, joka tuotepakkaus käsittää elektroniherkkää hopeahalidimateriaalia, kehitysliuosta, joka pelkistää hopeahalidit mainitussa elektroniherkässä hopeahalidimateriaalissa, ja kanavatransistorimatriisin.
10. Menetelmä kemiluminoinnin määrittämiseksi ioniherkällä kanavatransistorimatriisilla, joka menetelmä käsittää vaiheet: a) kiinnitetään näytteen molekyylit reaktiolokeroihin mainitussa matriisissa; b) saatetaan mainittu matriisi kontaktiin kemiluminontia aiheuttavan entsymaattisen molekyylin tai entsymaattisten molekyylien kanssa, jotka entsymaattiset molekyylit on konjugoitu molekyyleihin, joilla on spesifiset sitoutumisominaisuudet; c) inkuboidaan matriisia elektroniherkän hopeahalidimateriaalin ja kemiluminointisubstraatin kanssa; ja d) inkuboidaan matriisia kehitysliuoksella, joka pelkistää kemiluminointivalolle altistuneet hopeahalidit alkuainehopeaksi ja samanaikaisesti mitataan pH:ta kussakin erillisessä reaktiolokerossa, jolloin pH:n aleneminen osoittaa kemiluminoinnin läsnäolon reaktiolokerossa. δ CvJ CVJ cp CvJ X IX Q. CO o CO m C\J δ CvJ
FI20125303A 2012-03-19 2012-03-19 Menetelmä radioaktiivisuuden mittaamiseksi FI124531B (fi)

Priority Applications (4)

Application Number Priority Date Filing Date Title
FI20125303A FI124531B (fi) 2012-03-19 2012-03-19 Menetelmä radioaktiivisuuden mittaamiseksi
US14/382,512 US9869672B2 (en) 2012-03-19 2013-03-19 Method for measuring chemiluminescence
EP13764018.1A EP2839274B1 (en) 2012-03-19 2013-03-19 Method for measuring radioactivity or chemiluminescence
PCT/FI2013/050308 WO2013140040A1 (en) 2012-03-19 2013-03-19 Method for measuring radioactivity

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FI20125303A FI124531B (fi) 2012-03-19 2012-03-19 Menetelmä radioaktiivisuuden mittaamiseksi
FI20125303 2012-03-19

Publications (2)

Publication Number Publication Date
FI20125303A FI20125303A (fi) 2013-09-20
FI124531B true FI124531B (fi) 2014-09-30

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FI20125303A FI124531B (fi) 2012-03-19 2012-03-19 Menetelmä radioaktiivisuuden mittaamiseksi

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US (1) US9869672B2 (fi)
EP (1) EP2839274B1 (fi)
FI (1) FI124531B (fi)
WO (1) WO2013140040A1 (fi)

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4035155A (en) 1975-10-30 1977-07-12 University Patents, Inc. Method of high speed scintillation autoradiography
JPS63131057A (ja) * 1986-11-20 1988-06-03 Terumo Corp 酵素センサ
US5236816A (en) 1992-04-10 1993-08-17 Eastman Kodak Company Photographic developing solution and use thereof in the high contrast development of nucleated photographic elements
US7220550B2 (en) 1997-05-14 2007-05-22 Keensense, Inc. Molecular wire injection sensors
US5932398A (en) 1997-11-14 1999-08-03 Eastman Kodak Company Kit for roomlight processing of black-and-white photographic elements
US6670113B2 (en) 2001-03-30 2003-12-30 Nanoprobes Enzymatic deposition and alteration of metals
US20050123440A1 (en) * 2003-12-09 2005-06-09 Eastman Kodak Company Sensor for contaminants
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
EP2677308B1 (en) 2006-12-14 2017-04-26 Life Technologies Corporation Method for fabricating large scale FET arrays
JP2008170422A (ja) * 2006-12-15 2008-07-24 Fujifilm Corp 免疫学的測定方法
US9170228B2 (en) * 2007-06-08 2015-10-27 Bharath R. Takulapalli Nano structured field effect sensor and methods of forming and using same
JP2009162519A (ja) 2007-12-28 2009-07-23 Horiba Ltd 酵素活性測定装置
EP2261648B1 (en) * 2008-03-17 2014-05-21 Mitsubishi Chemical Medience Corporation Electric analysis method
US20100059391A1 (en) * 2008-08-14 2010-03-11 Agency For Science, Technology And Research Method for detecting biomolecules and use thereof
CA2765314A1 (en) 2009-06-10 2010-12-16 Helmholtz Zentrum Muenchen Deutsches Forschungszentrum Fuer Gesundheit U Nd Umwelt (Gmbh) Semiconductor biosensors

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Publication number Publication date
US20150065370A1 (en) 2015-03-05
US9869672B2 (en) 2018-01-16
WO2013140040A1 (en) 2013-09-26
EP2839274B1 (en) 2019-09-11
FI20125303A (fi) 2013-09-20
EP2839274A1 (en) 2015-02-25
EP2839274A4 (en) 2015-12-02

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