ES8802400A1 - Un metodo para producir una enzima que despolimeriza un polisacarido extracelular de erwina amylovora - Google Patents

Un metodo para producir una enzima que despolimeriza un polisacarido extracelular de erwina amylovora

Info

Publication number
ES8802400A1
ES8802400A1 ES551118A ES551118A ES8802400A1 ES 8802400 A1 ES8802400 A1 ES 8802400A1 ES 551118 A ES551118 A ES 551118A ES 551118 A ES551118 A ES 551118A ES 8802400 A1 ES8802400 A1 ES 8802400A1
Authority
ES
Spain
Prior art keywords
fireblight
compositions
treatment
phage
disease
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES551118A
Other languages
English (en)
Other versions
ES551118A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Microlife Technics Inc
Original Assignee
Microlife Technics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Microlife Technics Inc filed Critical Microlife Technics Inc
Publication of ES551118A0 publication Critical patent/ES551118A0/es
Publication of ES8802400A1 publication Critical patent/ES8802400A1/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/40—Viruses, e.g. bacteriophages
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/50—Isolated enzymes; Isolated proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01075—Poly(3-hydroxybutyrate) depolymerase (3.1.1.75)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Virology (AREA)
  • Plant Pathology (AREA)
  • General Engineering & Computer Science (AREA)
  • Environmental Sciences (AREA)
  • Dentistry (AREA)
  • Biochemistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Chemical And Physical Treatments For Wood And The Like (AREA)
  • Gasification And Melting Of Waste (AREA)
  • Fire-Extinguishing Compositions (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Fireproofing Substances (AREA)

Abstract

METODO PARA LA DESTRUCCION DE ERWINIA AMYLOVORA EN LAS PLANTAS. COMPRENDE LAS SIGUIENTES OPERACIONES: A) INFECTAR A LA ERWINIA AMYLOVORA CON UN FAGO QUE LISA A LA ERWINIA AMYLOVORA EN UN MEDIO DE CRECIMIENTO PARA PRODUCIR UNA ENZIMA, EFECTUANDOSE LA LISIS DURANTE UN PERIODO DE VARIAS HORAS; B) SEPARAR LA ENZIMA DEL MEDIO DE CRECIMIENTO; C) PURIFICAR LA ENZIMA POR FILTRACION, CROMATOGRAFIA O CAMBIO IONICO. LA ENZIMA PURIFICADA TIENE UN PESO MOLECULAR DE APROXIMADAMENTE 21.000 Y UNA ACTIVIDAD OPTIMA DE DESPOLIMERASA A PH6 Y A 30JC. DE APLICACION PARA EL TRATAMIENTO DE LA ENFERMEDAD DE FUEGO BACTERIANO EN LAS PLANTAS.
ES551118A 1984-10-18 1986-01-22 Un metodo para producir una enzima que despolimeriza un polisacarido extracelular de erwina amylovora Expired ES8802400A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US06/662,065 US4678750A (en) 1984-10-18 1984-10-18 Method and compositions for use in the treatment of fireblight

Publications (2)

Publication Number Publication Date
ES551118A0 ES551118A0 (es) 1988-05-16
ES8802400A1 true ES8802400A1 (es) 1988-05-16

Family

ID=24656252

Family Applications (2)

Application Number Title Priority Date Filing Date
ES547967A Expired ES8609461A1 (es) 1984-10-18 1985-10-17 Un procedimiento para preparar una composicion para el tra- tamiento de fuego bacteriano en plantas
ES551118A Expired ES8802400A1 (es) 1984-10-18 1986-01-22 Un metodo para producir una enzima que despolimeriza un polisacarido extracelular de erwina amylovora

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES547967A Expired ES8609461A1 (es) 1984-10-18 1985-10-17 Un procedimiento para preparar una composicion para el tra- tamiento de fuego bacteriano en plantas

Country Status (8)

Country Link
US (1) US4678750A (es)
EP (1) EP0182106B1 (es)
AT (1) ATE41729T1 (es)
AU (1) AU570574B2 (es)
CA (1) CA1246477A (es)
DE (2) DE3569075D1 (es)
ES (2) ES8609461A1 (es)
NZ (1) NZ213871A (es)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4822740A (en) * 1985-10-07 1989-04-18 Microlife Technics, Inc. Bacteria for expressing a polysaccharide depolymerase containing a novel recombinant plasmid
NZ256788A (en) * 1992-09-30 1996-11-26 Cornell Res Foundation Inc Conferring resistance to fire blight in pomaceous fruit scion or cultivars through transformation with genes encoding lytic proteins
WO1997039111A1 (en) * 1996-04-15 1997-10-23 Nymox Corporation Compositions containing bacteriophages and methods of using bacteriophages to treat infections
EP1059355A1 (fr) * 1999-06-11 2000-12-13 Agrostar Bactériocine, sa préparation et son utilisation
DE10149557A1 (de) * 2001-10-08 2003-04-10 Jobeck Gmbh Zubereitung und Verwendung dieser Zubereitung zur Bekämpfung und/oder Vorbeugung gegen den Erreger des Feuerbrandes Erwinia amylovora
GB0300597D0 (en) * 2003-01-10 2003-02-12 Microbiological Res Authority Phage biofilms
ES2527994T3 (es) 2003-07-23 2015-02-03 Biocontrol Limited Agentes terapéuticos que contienen bacteriófagos frente a P. aeruginosa
GB0704553D0 (en) * 2007-03-09 2007-04-18 Harper David R Beneficial effects of bacteriophage treatments
CA2711841A1 (en) 2008-01-10 2009-09-03 Trustees Of Boston University Engineered bacteriophages as adjuvants for antimicrobial agents and compositions and methods of use thereof
WO2010141135A2 (en) 2009-03-05 2010-12-09 Trustees Of Boston University Bacteriophages expressing antimicrobial peptides and uses thereof

Also Published As

Publication number Publication date
ES8609461A1 (es) 1986-08-01
CA1246477A (en) 1988-12-13
ES551118A0 (es) 1988-05-16
EP0182106A1 (en) 1986-05-28
DE3569075D1 (en) 1989-05-03
ATE41729T1 (de) 1989-04-15
NZ213871A (en) 1991-07-26
US4678750A (en) 1987-07-07
AU570574B2 (en) 1988-03-17
ES547967A0 (es) 1986-08-01
EP0182106B1 (en) 1989-03-29
AU4648985A (en) 1986-04-24
DE182106T1 (de) 1986-09-25

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