ES8701777A1 - Procedimiento de preparar sondas biologicas y oligonucleoticos. - Google Patents
Procedimiento de preparar sondas biologicas y oligonucleoticos.Info
- Publication number
- ES8701777A1 ES8701777A1 ES547038A ES547038A ES8701777A1 ES 8701777 A1 ES8701777 A1 ES 8701777A1 ES 547038 A ES547038 A ES 547038A ES 547038 A ES547038 A ES 547038A ES 8701777 A1 ES8701777 A1 ES 8701777A1
- Authority
- ES
- Spain
- Prior art keywords
- preparation
- groups
- containing modified
- modified adenine
- probes containing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Saccharide Compounds (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Steroid Compounds (AREA)
Abstract
METODO DE PREPARACION DE SONDAS BIOLOGICAS Y OLIGONUCLEOTIDOS. CONSISTE EN LA FIJACION DE UN MONOMERO, DIMERO O TRIMERO, POR SU EXTREMO 3K, SOBRE EL EXTREMO 5K DEL NUCLEOTIDO TERMINAL 3K DE LA SECUENCIA DESEADA, FIJADO SOBRE UN SOPORTE SOLIDO, SEGUIDA DE FIJACIONES SUCESIVAS, CADA VEZ SOBRE EL NUCLEOTIDO TERMINAL 5K DE LA CADENA OBTENIDA, DE MODO QUE SE REEMPLAZA AL MENOS UNA PARTE DE LOS GRUPOS ACIDO ADENILICO O ACIDO DESOXIADENILICO, SEGUN SE PREPARE UN FRAGMENTO DE ARN O DE ADN. ESTAS SONDAS TIENEN APLICACIONES PARA LA DETECCION NUCLEOTIDICAS PARTICULARES EN INGENIERIA GENETICA.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR8413095A FR2569407B1 (fr) | 1984-08-22 | 1984-08-22 | Sondes comportant des groupements adenine modifiee, leur preparation et leurs utilisations |
Publications (2)
Publication Number | Publication Date |
---|---|
ES8701777A1 true ES8701777A1 (es) | 1986-12-16 |
ES547038A0 ES547038A0 (es) | 1986-12-16 |
Family
ID=9307172
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES547038A Expired ES8701777A1 (es) | 1984-08-22 | 1985-08-21 | Procedimiento de preparar sondas biologicas y oligonucleoticos. |
Country Status (10)
Country | Link |
---|---|
US (1) | US4842996A (es) |
EP (1) | EP0176396B1 (es) |
JP (1) | JPS6169788A (es) |
AT (1) | ATE49976T1 (es) |
DE (1) | DE3575730D1 (es) |
DK (1) | DK380085A (es) |
ES (1) | ES8701777A1 (es) |
FR (1) | FR2569407B1 (es) |
GR (1) | GR852029B (es) |
PT (1) | PT81009B (es) |
Families Citing this family (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
NZ212024A (en) * | 1984-05-15 | 1988-08-30 | Smithkline Beckman Corp | A preparation procedure for polynucleotide hybridisation probes |
US4734363A (en) * | 1984-11-27 | 1988-03-29 | Molecular Diagnostics, Inc. | Large scale production of DNA probes |
FR2575751B1 (fr) * | 1985-01-08 | 1987-04-03 | Pasteur Institut | Nouveaux nucleosides de derives de l'adenosine, leur preparation et leurs applications biologiques |
FR2601956B1 (fr) * | 1986-07-22 | 1989-11-03 | Pasteur Institut | Nouveaux derives de desoxy-2' adenosine, leur procede d'obtention par voie de synthese et leurs applications biologiques |
GB9119378D0 (en) * | 1991-09-11 | 1991-10-23 | Medical Res Council | Improvements in oligonucleotide primers |
FR2750435B1 (fr) * | 1996-06-27 | 1998-08-28 | Appligene Oncor | Procede de formation de complexes d'hybridation dont la stabilite depend peu de la composition en base des deux molecules d'acides nucleiques hybridees |
US7947658B2 (en) * | 2003-09-12 | 2011-05-24 | University Of Massachusetts | RNA interference for the treatment of gain-of-function disorders |
US8680063B2 (en) * | 2003-09-12 | 2014-03-25 | University Of Massachusetts | RNA interference for the treatment of gain-of-function disorders |
CA2619534A1 (en) * | 2005-08-18 | 2007-02-22 | Alnylam Pharmaceuticals, Inc. | Methods and compositions for treating neurological disease |
US20070259827A1 (en) * | 2006-01-25 | 2007-11-08 | University Of Massachusetts | Compositions and methods for enhancing discriminatory RNA interference |
WO2008143774A2 (en) * | 2007-05-01 | 2008-11-27 | University Of Massachusetts | Methods and compositions for locating snp heterozygosity for allele specific diagnosis and therapy |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4556643A (en) * | 1982-07-26 | 1985-12-03 | Agracetus | Assay method and probe for polynucleotide sequences |
US4563417A (en) * | 1984-08-31 | 1986-01-07 | Miles Laboratories, Inc. | Nucleic acid hybridization assay employing antibodies to intercalation complexes |
-
1984
- 1984-08-22 FR FR8413095A patent/FR2569407B1/fr not_active Expired
-
1985
- 1985-08-21 AT AT85401665T patent/ATE49976T1/de active
- 1985-08-21 GR GR852029A patent/GR852029B/el unknown
- 1985-08-21 DK DK380085A patent/DK380085A/da not_active Application Discontinuation
- 1985-08-21 EP EP85401665A patent/EP0176396B1/fr not_active Expired - Lifetime
- 1985-08-21 ES ES547038A patent/ES8701777A1/es not_active Expired
- 1985-08-21 DE DE8585401665T patent/DE3575730D1/de not_active Expired - Lifetime
- 1985-08-22 US US06/768,042 patent/US4842996A/en not_active Expired - Fee Related
- 1985-08-22 JP JP60184986A patent/JPS6169788A/ja active Pending
- 1985-08-22 PT PT81009A patent/PT81009B/pt unknown
Also Published As
Publication number | Publication date |
---|---|
JPS6169788A (ja) | 1986-04-10 |
DE3575730D1 (de) | 1990-03-08 |
FR2569407B1 (fr) | 1989-02-17 |
PT81009B (fr) | 1987-02-06 |
PT81009A (fr) | 1985-09-01 |
US4842996A (en) | 1989-06-27 |
ES547038A0 (es) | 1986-12-16 |
DK380085D0 (da) | 1985-08-21 |
EP0176396A1 (fr) | 1986-04-02 |
DK380085A (da) | 1986-02-23 |
FR2569407A1 (fr) | 1986-02-28 |
EP0176396B1 (fr) | 1990-01-31 |
GR852029B (es) | 1985-12-19 |
ATE49976T1 (de) | 1990-02-15 |
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