ES8701777A1 - Procedimiento de preparar sondas biologicas y oligonucleoticos. - Google Patents

Procedimiento de preparar sondas biologicas y oligonucleoticos.

Info

Publication number
ES8701777A1
ES8701777A1 ES547038A ES547038A ES8701777A1 ES 8701777 A1 ES8701777 A1 ES 8701777A1 ES 547038 A ES547038 A ES 547038A ES 547038 A ES547038 A ES 547038A ES 8701777 A1 ES8701777 A1 ES 8701777A1
Authority
ES
Spain
Prior art keywords
preparation
groups
containing modified
modified adenine
probes containing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES547038A
Other languages
English (en)
Other versions
ES547038A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Institut Pasteur de Lille
Original Assignee
Institut Pasteur de Lille
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institut Pasteur de Lille filed Critical Institut Pasteur de Lille
Publication of ES8701777A1 publication Critical patent/ES8701777A1/es
Publication of ES547038A0 publication Critical patent/ES547038A0/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Steroid Compounds (AREA)

Abstract

METODO DE PREPARACION DE SONDAS BIOLOGICAS Y OLIGONUCLEOTIDOS. CONSISTE EN LA FIJACION DE UN MONOMERO, DIMERO O TRIMERO, POR SU EXTREMO 3K, SOBRE EL EXTREMO 5K DEL NUCLEOTIDO TERMINAL 3K DE LA SECUENCIA DESEADA, FIJADO SOBRE UN SOPORTE SOLIDO, SEGUIDA DE FIJACIONES SUCESIVAS, CADA VEZ SOBRE EL NUCLEOTIDO TERMINAL 5K DE LA CADENA OBTENIDA, DE MODO QUE SE REEMPLAZA AL MENOS UNA PARTE DE LOS GRUPOS ACIDO ADENILICO O ACIDO DESOXIADENILICO, SEGUN SE PREPARE UN FRAGMENTO DE ARN O DE ADN. ESTAS SONDAS TIENEN APLICACIONES PARA LA DETECCION NUCLEOTIDICAS PARTICULARES EN INGENIERIA GENETICA.
ES547038A 1984-08-22 1985-08-21 Procedimiento de preparar sondas biologicas y oligonucleoticos. Expired ES8701777A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR8413095A FR2569407B1 (fr) 1984-08-22 1984-08-22 Sondes comportant des groupements adenine modifiee, leur preparation et leurs utilisations

Publications (2)

Publication Number Publication Date
ES8701777A1 true ES8701777A1 (es) 1986-12-16
ES547038A0 ES547038A0 (es) 1986-12-16

Family

ID=9307172

Family Applications (1)

Application Number Title Priority Date Filing Date
ES547038A Expired ES8701777A1 (es) 1984-08-22 1985-08-21 Procedimiento de preparar sondas biologicas y oligonucleoticos.

Country Status (10)

Country Link
US (1) US4842996A (es)
EP (1) EP0176396B1 (es)
JP (1) JPS6169788A (es)
AT (1) ATE49976T1 (es)
DE (1) DE3575730D1 (es)
DK (1) DK380085A (es)
ES (1) ES8701777A1 (es)
FR (1) FR2569407B1 (es)
GR (1) GR852029B (es)
PT (1) PT81009B (es)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE45385T1 (de) * 1984-05-15 1989-08-15 Smithkline Beckman Corp Polynucleotid-hybridisierungsprobe.
US4734363A (en) * 1984-11-27 1988-03-29 Molecular Diagnostics, Inc. Large scale production of DNA probes
FR2575751B1 (fr) * 1985-01-08 1987-04-03 Pasteur Institut Nouveaux nucleosides de derives de l'adenosine, leur preparation et leurs applications biologiques
FR2601956B1 (fr) * 1986-07-22 1989-11-03 Pasteur Institut Nouveaux derives de desoxy-2' adenosine, leur procede d'obtention par voie de synthese et leurs applications biologiques
GB9119378D0 (en) * 1991-09-11 1991-10-23 Medical Res Council Improvements in oligonucleotide primers
FR2750435B1 (fr) * 1996-06-27 1998-08-28 Appligene Oncor Procede de formation de complexes d'hybridation dont la stabilite depend peu de la composition en base des deux molecules d'acides nucleiques hybridees
ES2808561T3 (es) * 2003-09-12 2021-03-01 Univ Massachusetts Interferencia por ARN para el tratamiento de trastornos de ganancia de función
US8680063B2 (en) 2003-09-12 2014-03-25 University Of Massachusetts RNA interference for the treatment of gain-of-function disorders
EP1937066A4 (en) 2005-08-18 2008-12-24 Alnylam Pharmaceuticals Inc METHOD AND COMPOSITIONS FOR THE TREATMENT OF NERVOUS DISEASES
US20070259827A1 (en) * 2006-01-25 2007-11-08 University Of Massachusetts Compositions and methods for enhancing discriminatory RNA interference
WO2008143774A2 (en) * 2007-05-01 2008-11-27 University Of Massachusetts Methods and compositions for locating snp heterozygosity for allele specific diagnosis and therapy

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4556643A (en) * 1982-07-26 1985-12-03 Agracetus Assay method and probe for polynucleotide sequences
US4563417A (en) * 1984-08-31 1986-01-07 Miles Laboratories, Inc. Nucleic acid hybridization assay employing antibodies to intercalation complexes

Also Published As

Publication number Publication date
FR2569407B1 (fr) 1989-02-17
ATE49976T1 (de) 1990-02-15
GR852029B (es) 1985-12-19
PT81009B (fr) 1987-02-06
DK380085D0 (da) 1985-08-21
EP0176396A1 (fr) 1986-04-02
EP0176396B1 (fr) 1990-01-31
ES547038A0 (es) 1986-12-16
FR2569407A1 (fr) 1986-02-28
DK380085A (da) 1986-02-23
JPS6169788A (ja) 1986-04-10
US4842996A (en) 1989-06-27
PT81009A (fr) 1985-09-01
DE3575730D1 (de) 1990-03-08

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