ES8607983A1 - Un metodo para producir una sonda de hibridacion de polinu- cleotidos - Google Patents

Un metodo para producir una sonda de hibridacion de polinu- cleotidos

Info

Publication number
ES8607983A1
ES8607983A1 ES543127A ES543127A ES8607983A1 ES 8607983 A1 ES8607983 A1 ES 8607983A1 ES 543127 A ES543127 A ES 543127A ES 543127 A ES543127 A ES 543127A ES 8607983 A1 ES8607983 A1 ES 8607983A1
Authority
ES
Spain
Prior art keywords
hybridization probes
polynucleotide hybridization
polynucleotide
probe regions
labeled
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES543127A
Other languages
English (en)
Other versions
ES543127A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SmithKline Beecham Corp
Original Assignee
SmithKline Beecham Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by SmithKline Beecham Corp filed Critical SmithKline Beecham Corp
Publication of ES8607983A1 publication Critical patent/ES8607983A1/es
Publication of ES543127A0 publication Critical patent/ES543127A0/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Emergency Protection Circuit Devices (AREA)
  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
  • Electroluminescent Light Sources (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

METODO PARA LA PRODUCCION DE SONDAS DE HIBRIDACION DE POLINUCLEOTIDOS. CONSISTE EN LA PROTECCION SELECTIVA DE UNA REGION DE SONDA DENTRO DE UN POLINUCLEOTIDO DE ADN O ARN, MONOCATENARIO, MEDIANTE HIBRIDACION DE LA REGION DE SONDA CON UNA SECUENCIA COMPLEMENTARIA, ANTES DE MODIFICAR EL POLINUCLEOTIDO; LA REGION DE SONDA SE PROTEGE SELECTIVAMENTE POR HIBRIDACION CON UNA SECUENCIA DE OLIGO- O POLINUCLEOTIDO COMPLEMENTARIA Y LA SONDA SE MODIFICA POR TRANSMISION O POR BIOTINILACION. EL POLINUCLEOTIDO MONOCATENARIO SE UNE, DIRECTA O INDIRECTAMENTE, A UN SOPORTE SOLIDO ANTES DE LA PROTECCION DE LA REGION DE SONDA. ESTAS SONDAS TIENEN APLICACIONES PARA DETECTAR, LOCALIZAR Y AISLAR OBJETIVOS DE NUCLEOTIDOS PARA FINES CLINICOS Y DE INVESTIGACION.
ES543127A 1984-05-15 1985-05-14 Un metodo para producir una sonda de hibridacion de polinu- cleotidos Expired ES8607983A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US61036384A 1984-05-15 1984-05-15

Publications (2)

Publication Number Publication Date
ES8607983A1 true ES8607983A1 (es) 1986-06-01
ES543127A0 ES543127A0 (es) 1986-06-01

Family

ID=24444714

Family Applications (1)

Application Number Title Priority Date Filing Date
ES543127A Expired ES8607983A1 (es) 1984-05-15 1985-05-14 Un metodo para producir una sonda de hibridacion de polinu- cleotidos

Country Status (15)

Country Link
EP (1) EP0172153B1 (es)
JP (1) JPS611388A (es)
KR (1) KR850008679A (es)
AT (1) ATE45385T1 (es)
AU (1) AU589362B2 (es)
DE (1) DE3572196D1 (es)
DK (1) DK213485A (es)
ES (1) ES8607983A1 (es)
FI (1) FI851919L (es)
GR (1) GR851153B (es)
IL (1) IL75142A0 (es)
NO (1) NO851919L (es)
NZ (1) NZ212024A (es)
PT (1) PT80451B (es)
ZA (1) ZA853621B (es)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3431219A1 (de) 1984-08-24 1986-03-06 Bayer Ag, 5090 Leverkusen Benzoylharnstoffe
WO1987004165A1 (en) * 1985-12-27 1987-07-16 Toray Industries, Inc. Dna probe and method of preparing the same
US4822731A (en) * 1986-01-09 1989-04-18 Cetus Corporation Process for labeling single-stranded nucleic acids and hybridizaiton probes
US4925785A (en) * 1986-03-07 1990-05-15 Biotechnica Diagnostics, Inc. Nucleic acid hybridization assays
JPS62260786A (ja) * 1986-05-08 1987-11-13 株式会社ヘキトク 燻瓦の製造ラインにおける焼成工程後の表面仕上げ装置
JP2783568B2 (ja) * 1987-07-31 1998-08-06 ジェン‐プローブ インコーポレイテッド 望ましくない交差反応を排除するためにオリゴヌクレオチドを使用するポリヌクレオチドの検定法
US5686579A (en) * 1988-06-21 1997-11-11 Hybrisens, Ltd. Use of antibody/antigen interactions to protect biologically active proteins and peptides
JPH0454194Y2 (es) * 1989-10-13 1992-12-18
CA2055755A1 (en) * 1990-11-22 1992-05-23 Toshihiko Kishimoto Method of immobilizing single-stranded dna on carrier at terminal

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1190838A (en) * 1981-07-17 1985-07-23 Cavit Akin Homogeneous nucleic acid hybridization diagnostics by non-radiative energy transfer
GB8306426D0 (en) * 1983-03-09 1983-04-13 Malcolm A D B Detecting polynucleotide sequence
CA1338856C (en) * 1983-07-05 1997-01-21 Jannis Stavrianopoulos In vivo labelling of polynucleotide sequences
US4737454A (en) * 1983-07-14 1988-04-12 Molecular Diagnostics, Inc. Fast photochemical method of labelling nucleic acids for detection purposes in hybridization assays
US4777129A (en) * 1983-12-12 1988-10-11 Molecular Diagnostics, Inc. Nucleic acid probe detectable by specific nucleic acid binding protein
US4840893A (en) * 1983-12-16 1989-06-20 Medisense, Inc. Electrochemical assay for nucleic acids and nucleic acid probes
FR2569407B1 (fr) * 1984-08-22 1989-02-17 Pasteur Institut Sondes comportant des groupements adenine modifiee, leur preparation et leurs utilisations

Also Published As

Publication number Publication date
NZ212024A (en) 1988-08-30
AU4245885A (en) 1985-11-21
JPS611388A (ja) 1986-01-07
KR850008679A (ko) 1985-12-21
FI851919L (fi) 1985-11-16
AU589362B2 (en) 1989-10-12
DK213485D0 (da) 1985-05-14
EP0172153A1 (en) 1986-02-19
NO851919L (no) 1985-11-18
ES543127A0 (es) 1986-06-01
GR851153B (es) 1985-11-25
ATE45385T1 (de) 1989-08-15
FI851919A0 (fi) 1985-05-14
PT80451B (en) 1987-01-14
DE3572196D1 (en) 1989-09-14
DK213485A (da) 1985-11-16
IL75142A0 (en) 1985-09-29
ZA853621B (en) 1986-04-30
EP0172153B1 (en) 1989-08-09
PT80451A (en) 1985-06-01

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