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Consejo Superior de Investigaciones Cientificas CSIC
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Consejo Superior de Investigaciones Cientificas CSIC
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Priority to ES547927ApriorityCriticalpatent/ES8605297A1/en
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Publication of ES547927A0publicationCriticalpatent/ES547927A0/en
Preparation Of Compounds By Using Micro-Organisms
(AREA)
Abstract
The polysaccharide is made by (a) treatment of the Azotobacter vinelandii strain 482 DSM with 2% glucose irradiated in Burk medium at pH 7.4 for 48 hours at ambient temp. (b) treatment of the resultant mutant with 2% mammitol irradiated in Burk medium at pH 7.4 for 160 hours (c) treatment with 1% glutamine and D,L-glutamic acid irradiated in the same medium at pH 7.8 for 268 hours (d) treatment with 2% arabinose and 0.5% D,L-glutamic acid irradiated in the same medium at pH 8 for 216 hours and (e) treatment with 2% arabinose irradiated in Burk medium at pH 8 for 120 hours.
Sucrose-mutase, immobilized sucrose-mutase and use of this immobilized sucrose-mutase for the production of isomaltulose(6-o-alpha-d-glucopyranosido-d fructose)