ES8307290A1 - Procedimiento para la produccion de un microorganismo que utiliza metanol geneticamente modificado. - Google Patents

Procedimiento para la produccion de un microorganismo que utiliza metanol geneticamente modificado.

Info

Publication number
ES8307290A1
ES8307290A1 ES512857A ES512857A ES8307290A1 ES 8307290 A1 ES8307290 A1 ES 8307290A1 ES 512857 A ES512857 A ES 512857A ES 512857 A ES512857 A ES 512857A ES 8307290 A1 ES8307290 A1 ES 8307290A1
Authority
ES
Spain
Prior art keywords
methanol
microorganisms
production
formaldehyde
single cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES512857A
Other languages
English (en)
Other versions
ES512857A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Imperial Chemical Industries Ltd
Original Assignee
Imperial Chemical Industries Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Imperial Chemical Industries Ltd filed Critical Imperial Chemical Industries Ltd
Publication of ES512857A0 publication Critical patent/ES512857A0/es
Publication of ES8307290A1 publication Critical patent/ES8307290A1/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/32—Processes using, or culture media containing, lower alkanols, i.e. C1 to C6
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/746—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for lactic acid bacteria (Streptococcus; Lactococcus; Lactobacillus; Pediococcus; Enterococcus; Leuconostoc; Propionibacterium; Bifidobacterium; Sporolactobacillus)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/78—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Pseudomonas

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

PROCEDIMIENTO PARA LA OBTENCION DE UN MICROORGANISMO QUE UTILIZA METANOL GENETICAMENTE MODIFICADO, SUSTITUYENDO EL GEN O GENES QUE CODIFICAN LA ACTIVIDAD DE DEHIDROGENASA U OXIDASA DE METANOL POR MATERIAL DNA. CONSISTE EN OBTENER UNA REPLICA QUE CONTIENE EL GEN ~AADH~B, A PARTIR DE UN MICROORGANISMO QUE TIENE DICHO GEN, POR MEDIOS ~AIN VITRO~B O ~AIN VIVO~B; PRODUCIR UN MUTANTE DE MICROORGANISMO QUE UTILIZA METANOL DEFICIENTE EN EL GEN O GENES QUE CODIFICAN LA ACTIVIDAD ENZIMATICA DE HIDROGENASA U OXIDASA DE METANOL; Y TRANSFERIR EL GEN, SOBRE UN VECTOR ADECUADO, A OTRO MICROORGANISMO. ESTOS MICROORGANISMOS TIENEN APLICACIONES EN PROCESOS PARA LA PRODUCCION DE SCP.
ES512857A 1981-06-04 1982-06-04 Procedimiento para la produccion de un microorganismo que utiliza metanol geneticamente modificado. Expired ES8307290A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB8117191 1981-06-04

Publications (2)

Publication Number Publication Date
ES512857A0 ES512857A0 (es) 1983-07-01
ES8307290A1 true ES8307290A1 (es) 1983-07-01

Family

ID=10522288

Family Applications (1)

Application Number Title Priority Date Filing Date
ES512857A Expired ES8307290A1 (es) 1981-06-04 1982-06-04 Procedimiento para la produccion de un microorganismo que utiliza metanol geneticamente modificado.

Country Status (7)

Country Link
EP (1) EP0066994A3 (es)
JP (1) JPS57208989A (es)
AU (1) AU8420582A (es)
DK (1) DK251782A (es)
ES (1) ES8307290A1 (es)
NO (1) NO821818L (es)
NZ (1) NZ200768A (es)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0098967B1 (de) * 1982-06-11 1989-08-09 Hoechst Aktiengesellschaft Verfahren zur Herstellung von obligat methylotrophen, Fremd-DNA exprimierenden Bakterien und dafür geeignete Plasmide und Wirtsorganismen
NL8303082A (nl) * 1983-09-05 1985-04-01 Delft Tech Hogeschool Methanol dehydrogenase enzym; werkwijze voor het kweken van micro-organismen; chemische produkten; door micro-organismen gekatalyseerde epoxydaties of hydroxyleringen; methylotrofe micro-organismen.
US5240838A (en) * 1984-07-27 1993-08-31 Internationale Otrool Maatschappij "Octropa" B.V. Regulatory sequences of alcohol oxidase (MOX) and dihydroxyacetonesynthase (DAS) of Hansenula polymorpha
EP0423890A3 (en) * 1984-07-27 1991-07-03 Unilever Nv Use of oxidoreductases in bleaching and/or detergent compositions and their preparation by microorganisms engineered by recombinant dna technology
US5741672A (en) * 1984-07-27 1998-04-21 Unilever Patent Holdings B.V. Expression and production of polypeptides using the promoters of the hansenula polymorpha MOX and DAS genes
US4818599A (en) * 1986-10-21 1989-04-04 E. I. Dupont De Nemours And Company Polyester fiberfill
WO1994028154A1 (en) * 1993-05-27 1994-12-08 The Nutrasweet Company Methods for increasing carbon conversion efficiency in microorganisms
US6911332B2 (en) 2002-06-12 2005-06-28 Ajinomoto Co., Inc. Isolated polynucleotides encoding d-arabino-3-hexulose-6-phosphate synthases from Methylophilus methylotrophus
CN100591765C (zh) 2002-07-12 2010-02-24 新英格兰生物实验室公司 重组II型限制性内切核酸酶MmeI和相关内切核酸酶以及制备这些酶的方法
US7029893B2 (en) 2003-02-28 2006-04-18 Ajinomoto Co., Inc. Polynucleotides encoding polypeptides involved in amino acid biosynthesis in methylophilus methylotrophus
US7026149B2 (en) 2003-02-28 2006-04-11 Ajinomoto Co., Inc. Polynucleotides encoding polypeptides involved in the stress response to environmental changes in Methylophilus methylotrophus
US7060475B2 (en) 2003-02-28 2006-06-13 Ajinomoto Co., Inc. Polynucleotides encoding polypeptides involved in intermediates metabolism of central metabolic pathway in methylophilus methylotrophus
US9932611B2 (en) * 2012-10-22 2018-04-03 Genomatica, Inc. Microorganisms and methods for enhancing the availability of reducing equivalents in the presence of methanol, and for producing succinate related thereto

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2003926B (en) * 1977-09-08 1982-03-17 Ici Ltd Microbiological process

Also Published As

Publication number Publication date
JPS57208989A (en) 1982-12-22
NO821818L (no) 1982-12-06
ES512857A0 (es) 1983-07-01
AU8420582A (en) 1982-12-09
EP0066994A2 (en) 1982-12-15
DK251782A (da) 1982-12-05
NZ200768A (en) 1985-07-31
EP0066994A3 (en) 1984-02-01

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