ES8205264A1 - Procedimiento para producir y seleccionar un bacteriofago recombinante que contiene un rasgo genetico deseado. - Google Patents
Procedimiento para producir y seleccionar un bacteriofago recombinante que contiene un rasgo genetico deseado.Info
- Publication number
- ES8205264A1 ES8205264A1 ES498333A ES498333A ES8205264A1 ES 8205264 A1 ES8205264 A1 ES 8205264A1 ES 498333 A ES498333 A ES 498333A ES 498333 A ES498333 A ES 498333A ES 8205264 A1 ES8205264 A1 ES 8205264A1
- Authority
- ES
- Spain
- Prior art keywords
- recombinant bacteriophage
- bacillus
- incubating
- dna
- production
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
- C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
PROCEDIMIENTO PARA LA OBTENCION Y SELECCION DE UN BACTERIOFAGO RECOMBINANTE QUE CONTIENE UN RASGO GENETICO DESEADO, ADECUADO PARA SER UTILIZADO EN LA TRANSFORMACION HETEROLOGA DE UN MICROOGANISMO ANFITRION DE BACILLUS. CONSITE EN OBTENER FRAGMENTOS GENETICOS QUE CODIFICAN EL RASGO GENETICO DESEADO; AISLAR FRAGMENTOS DE DNA DE UN BACTERIOFAGO; LIGAR LOS FRAGMENTOS GENETICOS CON EL DNA; INCUBAR LA MEZCLA CON DNA AISLADO DE UN SEGUNDO MICROORGANISMO DE BACILLUS QUE NO CONTIENE LOS FRAGMENTOS GENETICOS ANTES CITADOS; INCUBAR ESTA MEZCLA CON EL MICROORGANISMO ANFITRION DE BACILLUS, LISOGENICO PARA EL BACTERIOFAGO Y AUXOTROPICO PARA LA NECESIDAD DE CRECIMIENTO; SELECCIONAR EL BACILLUS ANFITRION TRANSFORMADO; DETERMINAR LA PRESENCIA DEL RASGO GENETICO DESEADO, Y OBTENER A PARTIR DEL MISMO EL BACTERIOFAGO RECOMBINANTE QUE CONTIENE EL RASGO GENETICO DESEADO POR INDUCCION DEL BACILLUS ANFITRON PROTOTROFICO.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US11004280A | 1980-01-07 | 1980-01-07 |
Publications (2)
Publication Number | Publication Date |
---|---|
ES498333A0 ES498333A0 (es) | 1982-06-01 |
ES8205264A1 true ES8205264A1 (es) | 1982-06-01 |
Family
ID=22330923
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES498333A Expired ES8205264A1 (es) | 1980-01-07 | 1981-01-05 | Procedimiento para producir y seleccionar un bacteriofago recombinante que contiene un rasgo genetico deseado. |
Country Status (9)
Country | Link |
---|---|
EP (1) | EP0032238B1 (es) |
JP (2) | JPS56113293A (es) |
AT (1) | ATE16943T1 (es) |
DE (1) | DE3071292D1 (es) |
DK (1) | DK3781A (es) |
ES (1) | ES8205264A1 (es) |
IL (1) | IL61837A (es) |
NO (1) | NO159863C (es) |
ZA (1) | ZA8142B (es) |
Families Citing this family (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
NO159863C (no) * | 1980-01-07 | 1989-02-15 | Univ Rochester | Fremgangsm te for fremstilling og seleksjon av en rant bakteriofag som inneholder et genetisk fragment og koder for alfa-amylase, egnet for bruk i heterolog transformering av en bacillus-verts-mikroorganisme. |
EP0074553A3 (en) * | 1981-09-11 | 1985-01-09 | The University Of Rochester | Method of increasing the yield of a product by altering a microorganism |
JPS60145685A (ja) | 1984-01-09 | 1985-08-01 | Nec Corp | 分布帰還型半導体レ−ザ |
JPS592689A (ja) * | 1982-06-04 | 1984-01-09 | Handai Biseibutsubiyou Kenkyukai | 強力な遺伝子発現能を有する新規レプリコンの作成法 |
FR2533583A1 (fr) * | 1982-09-24 | 1984-03-30 | Centre Nat Rech Scient | Nouvel adn recombinant utile dans la preparation d'a-amylase |
FR2537602B2 (fr) * | 1982-09-24 | 1986-10-24 | Centre Nat Rech Scient | Nouvel adn recombinant perfectionne utile dans la preparation d'a-amylase par bacillus subtilis |
ATE48156T1 (de) * | 1982-11-01 | 1989-12-15 | Miles Inc | Verfahren zur heterologen klonierung eines gens in einem bacillus-mikroorganismus. |
US5624829A (en) * | 1984-07-03 | 1997-04-29 | Gist-Brocades, B.V. | Transformed industrial bacillus strains and methods for making and using them |
NZ208806A (en) * | 1983-07-06 | 1988-07-28 | Gist Brocades Nv | Genetic engineering of industrial microorganism species: readily-transformable host used as intermediate in transfer of dna to the industrial species; plasmids |
US4801537A (en) * | 1984-06-08 | 1989-01-31 | Genex Corporation | Vector for expression of polypeptides in bacilli |
AU5668486A (en) * | 1985-03-29 | 1986-10-23 | Biotechnica International Inc. | Secretion vector |
JP2637532B2 (ja) * | 1987-02-27 | 1997-08-06 | ギスト ブロカデス ナームローゼ フェンノートチャップ | 原核微生物の染色体dnaにおける安定した遺伝子増幅 |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
NO159863C (no) * | 1980-01-07 | 1989-02-15 | Univ Rochester | Fremgangsm te for fremstilling og seleksjon av en rant bakteriofag som inneholder et genetisk fragment og koder for alfa-amylase, egnet for bruk i heterolog transformering av en bacillus-verts-mikroorganisme. |
-
1980
- 1980-12-15 NO NO803779A patent/NO159863C/no unknown
- 1980-12-24 AT AT80108211T patent/ATE16943T1/de not_active IP Right Cessation
- 1980-12-24 DE DE8080108211T patent/DE3071292D1/de not_active Expired
- 1980-12-24 EP EP80108211A patent/EP0032238B1/en not_active Expired
-
1981
- 1981-01-01 IL IL61837A patent/IL61837A/xx unknown
- 1981-01-05 ZA ZA00810042A patent/ZA8142B/xx unknown
- 1981-01-05 ES ES498333A patent/ES8205264A1/es not_active Expired
- 1981-01-06 JP JP20581A patent/JPS56113293A/ja active Granted
- 1981-01-06 DK DK3781A patent/DK3781A/da not_active Application Discontinuation
-
1986
- 1986-12-26 JP JP61308944A patent/JPS62163694A/ja active Pending
Also Published As
Publication number | Publication date |
---|---|
JPS6246153B2 (es) | 1987-09-30 |
IL61837A (en) | 1984-08-31 |
NO159863B (no) | 1988-11-07 |
ES498333A0 (es) | 1982-06-01 |
JPS62163694A (ja) | 1987-07-20 |
ZA8142B (en) | 1982-07-28 |
NO159863C (no) | 1989-02-15 |
NO803779L (no) | 1981-07-08 |
EP0032238A3 (en) | 1982-01-20 |
IL61837A0 (en) | 1981-02-27 |
DK3781A (da) | 1981-07-08 |
JPS56113293A (en) | 1981-09-07 |
EP0032238B1 (en) | 1985-12-11 |
ATE16943T1 (de) | 1985-12-15 |
DE3071292D1 (en) | 1986-01-23 |
EP0032238A2 (en) | 1981-07-22 |
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