ES427568A1 - Procedure for dying a tissue for microscopic examination. (Machine-translation by Google Translate, not legally binding) - Google Patents
Procedure for dying a tissue for microscopic examination. (Machine-translation by Google Translate, not legally binding)Info
- Publication number
- ES427568A1 ES427568A1 ES427568A ES427568A ES427568A1 ES 427568 A1 ES427568 A1 ES 427568A1 ES 427568 A ES427568 A ES 427568A ES 427568 A ES427568 A ES 427568A ES 427568 A1 ES427568 A1 ES 427568A1
- Authority
- ES
- Spain
- Prior art keywords
- tissue
- translation
- procedure
- machine
- legally binding
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 238000000034 method Methods 0.000 title abstract 2
- JIAARYAFYJHUJI-UHFFFAOYSA-L zinc dichloride Chemical compound [Cl-].[Cl-].[Zn+2] JIAARYAFYJHUJI-UHFFFAOYSA-L 0.000 abstract 4
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 abstract 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 abstract 2
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 abstract 2
- 235000005074 zinc chloride Nutrition 0.000 abstract 2
- 239000011592 zinc chloride Substances 0.000 abstract 2
- 239000002253 acid Substances 0.000 abstract 1
- 239000007864 aqueous solution Substances 0.000 abstract 1
- 238000004043 dyeing Methods 0.000 abstract 1
- WSFSSNUMVMOOMR-NJFSPNSNSA-N methanone Chemical compound O=[14CH2] WSFSSNUMVMOOMR-NJFSPNSNSA-N 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 239000000243 solution Substances 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/0231—Chemically defined matrices, e.g. alginate gels, for immobilising, holding or storing cells, tissue or organs for preservation purposes; Chemically altering or fixing cells, tissue or organs, e.g. by cross-linking, for preservation purposes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N2001/305—Fixative compositions
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Physics & Mathematics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Dispersion Chemistry (AREA)
- Molecular Biology (AREA)
- Dentistry (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
Procedure for dyeing a tissue for microscopic examination, characterized in that it comprises the operations of: (a) immersing frozen tissue in a 0 to 20% w/v solution of a mixture of trichloroacetic acid, zinc chloride and formaldehyde, in which the weight ratio of zinc chloride or trichloroacetic acid ranges from about 2: 1 to about 4: 1 and the weight ratio of formaldehyde or trochloracetic acid is from about 2: 1 to about 10: 1 in a lower aqueous ethanol, in which the ratio of alkanol to water tenol variety ranges from about from 1: 1 to about 1: 3, and (b) after immersing the tissue in at least one time in an aqueous solution, comprising 0.1 to 2.4 hemotoxylin. (Machine-translation by Google Translate, not legally binding)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US37327673A | 1973-06-25 | 1973-06-25 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES427568A1 true ES427568A1 (en) | 1976-07-16 |
Family
ID=23471724
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES427568A Expired ES427568A1 (en) | 1973-06-25 | 1974-06-22 | Procedure for dying a tissue for microscopic examination. (Machine-translation by Google Translate, not legally binding) |
Country Status (16)
| Country | Link |
|---|---|
| JP (1) | JPS5049026A (en) |
| AU (1) | AU7049174A (en) |
| BE (1) | BE816786R (en) |
| BR (1) | BR7405213D0 (en) |
| CA (1) | CA1037366A (en) |
| DD (1) | DD112519A5 (en) |
| DE (1) | DE2429647A1 (en) |
| DK (1) | DK338574A (en) |
| ES (1) | ES427568A1 (en) |
| FI (1) | FI193074A (en) |
| FR (1) | FR2234558B2 (en) |
| HU (1) | HU168582B (en) |
| NL (1) | NL7408556A (en) |
| NO (1) | NO742274L (en) |
| SE (1) | SE7407899L (en) |
| ZA (1) | ZA743995B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH068818B2 (en) * | 1987-11-27 | 1994-02-02 | サクラ精機株式会社 | Fixative for biopsy |
| EP1605244A1 (en) * | 2004-06-09 | 2005-12-14 | Boon, Mathilde Elisabeth | Fixative composition |
| CN110823666A (en) * | 2019-11-27 | 2020-02-21 | 李雄 | Application of hematoxylin in preparation of staining agent for reducing color fading of pathological conventional section after staining |
-
1974
- 1974-06-14 SE SE7407899A patent/SE7407899L/ not_active Application Discontinuation
- 1974-06-20 DE DE2429647A patent/DE2429647A1/en active Pending
- 1974-06-21 CA CA203,034A patent/CA1037366A/en not_active Expired
- 1974-06-21 NO NO742274A patent/NO742274L/no unknown
- 1974-06-21 ZA ZA00743995A patent/ZA743995B/en unknown
- 1974-06-22 ES ES427568A patent/ES427568A1/en not_active Expired
- 1974-06-24 BE BE145822A patent/BE816786R/en active
- 1974-06-24 HU HUAI236A patent/HU168582B/hu unknown
- 1974-06-24 DD DD179409A patent/DD112519A5/xx unknown
- 1974-06-24 DK DK338574A patent/DK338574A/da unknown
- 1974-06-24 FI FI1930/74A patent/FI193074A/fi unknown
- 1974-06-25 BR BR5213/74A patent/BR7405213D0/en unknown
- 1974-06-25 FR FR7422137A patent/FR2234558B2/fr not_active Expired
- 1974-06-25 JP JP49072710A patent/JPS5049026A/ja active Pending
- 1974-06-25 NL NL7408556A patent/NL7408556A/xx unknown
- 1974-06-25 AU AU70491/74A patent/AU7049174A/en not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| FR2234558B2 (en) | 1977-02-11 |
| FR2234558A2 (en) | 1975-01-17 |
| SE7407899L (en) | 1974-12-27 |
| FI193074A (en) | 1974-12-26 |
| ZA743995B (en) | 1975-06-25 |
| BE816786R (en) | 1974-12-24 |
| DK338574A (en) | 1975-02-24 |
| NO742274L (en) | 1975-01-20 |
| DE2429647A1 (en) | 1975-01-16 |
| BR7405213D0 (en) | 1975-01-28 |
| CA1037366A (en) | 1978-08-29 |
| NL7408556A (en) | 1974-12-30 |
| AU7049174A (en) | 1976-01-08 |
| DD112519A5 (en) | 1975-04-12 |
| JPS5049026A (en) | 1975-05-01 |
| HU168582B (en) | 1976-06-28 |
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