ES2735024T3 - Glicerol-3-fosfato deshidrogenasa para la producción de butanol - Google Patents
Glicerol-3-fosfato deshidrogenasa para la producción de butanol Download PDFInfo
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- ES2735024T3 ES2735024T3 ES14719570T ES14719570T ES2735024T3 ES 2735024 T3 ES2735024 T3 ES 2735024T3 ES 14719570 T ES14719570 T ES 14719570T ES 14719570 T ES14719570 T ES 14719570T ES 2735024 T3 ES2735024 T3 ES 2735024T3
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- gpd
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- butanol
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- C12P7/00—Preparation of oxygen-containing organic compounds
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- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/16—Butanols
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
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- C12Y101/01008—Glycerol-3-phosphate dehydrogenase (NAD+) (1.1.1.8)
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- C12Y101/01094—Glycerol-3-phosphate dehydrogenase (NAD(P)+)(1.1.1.94)
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
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| US9909148B2 (en) | 2011-12-30 | 2018-03-06 | Butamax Advanced Biofuels Llc | Fermentative production of alcohols |
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| WO2018106656A1 (en) | 2016-12-06 | 2018-06-14 | Danisco Us Inc | Truncated lpmo enzymes and use thereof |
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Family Cites Families (84)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| AU2388295A (en) | 1994-04-15 | 1995-11-10 | Midwest Research Institute | Recombinant zymomonas for pentose fermentation |
| US5514583A (en) | 1994-04-15 | 1996-05-07 | Midwest Research Institute | Recombinant zymomonas for pentose fermentation |
| US5712133A (en) | 1994-04-15 | 1998-01-27 | Midwest Research Institute | Pentose fermentation by recombinant zymomonas |
| US5686276A (en) | 1995-05-12 | 1997-11-11 | E. I. Du Pont De Nemours And Company | Bioconversion of a fermentable carbon source to 1,3-propanediol by a single microorganism |
| ID21487A (id) * | 1996-11-13 | 1999-06-17 | Du Pont | Metoda pembuatan 1,3 - propandiol dengan organisme rekombinan |
| EP1020515A3 (en) | 1999-01-18 | 2002-08-07 | DAICEL CHEMICAL INDUSTRIES, Ltd. | Amino alcohol dehydrogenase, its production and use |
| US7223575B2 (en) | 2000-05-01 | 2007-05-29 | Midwest Research Institute | Zymomonas pentose-sugar fermenting strains and uses thereof |
| US20030055485A1 (en) | 2001-09-17 | 2003-03-20 | Intra Therapeutics, Inc. | Stent with offset cell geometry |
| US7574601B2 (en) | 2004-08-24 | 2009-08-11 | Microsoft Corporation | Securely inspecting electronic messages |
| BRPI0612944B1 (pt) | 2005-04-12 | 2017-11-28 | E.I.Du Pont De Nemours And Company | Method of production of target substance |
| US9297028B2 (en) | 2005-09-29 | 2016-03-29 | Butamax Advanced Biofuels Llc | Fermentive production of four carbon alcohols |
| US8273558B2 (en) | 2005-10-26 | 2012-09-25 | Butamax(Tm) Advanced Biofuels Llc | Fermentive production of four carbon alcohols |
| US8945899B2 (en) | 2007-12-20 | 2015-02-03 | Butamax Advanced Biofuels Llc | Ketol-acid reductoisomerase using NADH |
| EP1948814B1 (en) | 2005-10-26 | 2018-11-21 | Butamax (TM) Advanced Biofuels LLC | Fermentive production of four carbon alcohols |
| US9303225B2 (en) | 2005-10-26 | 2016-04-05 | Butamax Advanced Biofuels Llc | Method for the production of isobutanol by recombinant yeast |
| US8956850B2 (en) | 2008-06-05 | 2015-02-17 | Butamax Advanced Biofuels Llc | Enhanced pyruvate to acetolactate conversion in yeast |
| US8828704B2 (en) | 2006-05-02 | 2014-09-09 | Butamax Advanced Biofuels Llc | Fermentive production of four carbon alcohols |
| US8962298B2 (en) | 2006-05-02 | 2015-02-24 | Butamax Advanced Biofuels Llc | Recombinant host cell comprising a diol dehydratase |
| US7659104B2 (en) | 2006-05-05 | 2010-02-09 | E.I. Du Pont De Nemours And Company | Solvent tolerant microorganisms and methods of isolation |
| US7541173B2 (en) | 2006-06-15 | 2009-06-02 | E.I. Du Pont De Nemours And Company | Solvent tolerant microorganisms and methods of isolation |
| US7741119B2 (en) | 2006-09-28 | 2010-06-22 | E. I. Du Pont De Nemours And Company | Xylitol synthesis mutant of xylose-utilizing zymomonas for ethanol production |
| US8017364B2 (en) | 2006-12-12 | 2011-09-13 | Butamax(Tm) Advanced Biofuels Llc | Solvent tolerant microorganisms |
| NZ579780A (en) | 2007-04-18 | 2012-07-27 | Butamax Advanced Biofuels Llc | Fermentive production of isobutanol using highly active ketol-acid reductoisomerase enzymes |
| US8426174B2 (en) | 2007-05-02 | 2013-04-23 | Butamax(Tm) Advanced Biofuels Llc | Method for the production of 2-butanol |
| EP2175714A4 (en) * | 2007-07-10 | 2011-01-26 | Monsanto Technology Llc | TRANSGENIC PLANTS WITH IMPROVED AGRONOMIC CHARACTERISTICS |
| EP2060632A1 (en) | 2007-10-29 | 2009-05-20 | Technische Universität Berlin | Method of modifying a yeast cell for the production of ethanol |
| US7897396B2 (en) | 2007-10-30 | 2011-03-01 | E.I. Du Pont De Nemours And Company | Zymomonas with improved ethanol production in medium containing concentrated sugars and acetate |
| ES2575413T3 (es) | 2007-12-20 | 2016-06-28 | Butamax (Tm) Advanced Biofuels Llc | Cetol-ácido reductoisomerasa que utiliza NADH |
| US8518678B2 (en) | 2007-12-21 | 2013-08-27 | Butamax(Tm) Advanced Biofuels Llc | Strain comprising increased expression of a CFA coding region for butanol production |
| US8372612B2 (en) | 2007-12-21 | 2013-02-12 | Butamax(Tm) Advanced Biofuels Llc | Production of four carbon alcohols using improved strain |
| US8455239B2 (en) * | 2007-12-23 | 2013-06-04 | Gevo, Inc. | Yeast organism producing isobutanol at a high yield |
| CN102037120B (zh) | 2008-03-27 | 2013-09-11 | 纳幕尔杜邦公司 | 具有改善的木糖利用率的发酵单胞菌属 |
| US8188250B2 (en) | 2008-04-28 | 2012-05-29 | Butamax(Tm) Advanced Biofuels Llc | Butanol dehydrogenase enzyme from the bacterium Achromobacter xylosoxidans |
| US8389252B2 (en) | 2008-05-12 | 2013-03-05 | Butamax(Tm) Advanced Biofuels Llc | Yeast strain for production of four carbon alcohols |
| US8906666B2 (en) | 2008-05-22 | 2014-12-09 | Butamax Advanced Biofuels Llc | Engineering resistance to aliphatic alcohols |
| AU2009256148B2 (en) | 2008-06-04 | 2014-11-27 | Butamax(Tm) Advanced Biofuels Llc | A method for producing butanol using two-phase extractive fermentation |
| CA2735948A1 (en) | 2008-09-29 | 2011-04-01 | Butamaxtm Advanced Biofuels Llc | Enhanced pyruvate to 2,3-butanediol conversion in lactic acid bacteria |
| AU2009296218A1 (en) | 2008-09-29 | 2010-04-01 | Butamax(Tm) Advanced Biofuels Llc | Enhanced dihydroxy-acid dehydratase activity in lactic acid bacteria |
| BRPI0913680A2 (pt) | 2008-09-29 | 2019-09-24 | Butamax Advanced Biofuels Llc | "célula bacteriana ácido-láctica, método para aumentar a atividade de um polipeptídeo dihidroxi-ácido desidratase heterólogo em uma célula bacteriana ácido-láctica e método de produção de insobutanol" |
| CA2735690A1 (en) | 2008-09-29 | 2010-04-01 | Dennis Flint | Identification and use of bacterial [2fe-2s] dihydroxy-acid dehydratases |
| CN102186983A (zh) | 2008-09-29 | 2011-09-14 | 布特马斯先进生物燃料有限责任公司 | 酵母中提高的异源Fe-S酶活性 |
| WO2010062597A1 (en) | 2008-10-27 | 2010-06-03 | Butamax™ Advanced Biofuels LLC | Carbon pathway optimized production hosts for the production of isobutanol |
| US8828694B2 (en) | 2008-11-13 | 2014-09-09 | Butamax Advanced Biofuels Llc | Production of isobutanol in yeast mitochondria |
| US8465964B2 (en) | 2008-11-13 | 2013-06-18 | Butamax (TM) Advanced Biofules LLC | Increased production of isobutanol in yeast with reduced mitochondrial amino acid biosynthesis |
| US8652823B2 (en) | 2008-12-03 | 2014-02-18 | Butamax(Tm) Advanced Biofuels Llc | Strain for butanol production with increased membrane unsaturated trans fatty acids |
| US8247208B2 (en) | 2008-12-22 | 2012-08-21 | Alliance For Sustainable Energy Llc | Zymomonas with improved xylose utilization in stress conditions |
| US8795992B2 (en) | 2008-12-29 | 2014-08-05 | Butamax Advanced Biofuels Llc | Yeast with increased butanol tolerance involving cell wall integrity pathway |
| US8557562B2 (en) | 2008-12-29 | 2013-10-15 | Butamax(Tm) Advanced Biofuels Llc | Yeast with increased butanol tolerance involving filamentous growth response |
| US8455225B2 (en) | 2008-12-29 | 2013-06-04 | Butamax Advanced Biofuels Llc | Yeast with increased butanol tolerance involving high osmolarity/glycerol response pathway |
| US8741839B2 (en) * | 2009-01-18 | 2014-06-03 | The Board Of Trustees Of The Leland Stanford Junior University | Polypeptides targeting vascular endothelial growth factor receptor-2 and αvβ3 integrin |
| US8614085B2 (en) | 2009-02-27 | 2013-12-24 | Butamax(Tm) Advanced Biofuels Llc | Yeast with increased butanol tolerance involving a multidrug efflux pump gene |
| JP2012523245A (ja) | 2009-04-13 | 2012-10-04 | ビュータマックス・アドバンスド・バイオフューエルズ・エルエルシー | 抽出発酵を用いてブタノールを生成するための方法 |
| US20120058541A1 (en) | 2009-05-22 | 2012-03-08 | Butamax(Tm) Advanced Biofuels Llc | Engineering resistance to aliphatic alcohols |
| EP2277989A1 (en) | 2009-07-24 | 2011-01-26 | Technische Universiteit Delft | Fermentative glycerol-free ethanol production |
| EP2483400A1 (en) | 2009-09-29 | 2012-08-08 | Butamax(tm) Advanced Biofuels LLC | Improved flux to acetolactate-derived products in lactic acid bacteria |
| BR112012006939A2 (pt) | 2009-09-29 | 2015-09-08 | Butamax Tm Advanced Biofuels | célula hospedeira de produção de levedura recombinante e método para a produção de um produto selecionado do grupo que consiste em 2,3-butanodiol, isobutanol, 2-butanol, 1-butanol, 2-butanona, valina, leucina, ácido lático, ácido málico, álcool, isoampilico e isoprenoides |
| CA2775892A1 (en) | 2009-09-29 | 2011-04-07 | Butamax(Tm) Advanced Biofuels Llc | Fermentive production of isobutanol using highly effective ketol-acid reductoisomerase enzymes |
| US20110195505A1 (en) | 2009-10-08 | 2011-08-11 | Butamax(Tm) Advanced Biofuels Llc | Bacterial strains for butanol production |
| US8637289B2 (en) | 2009-12-29 | 2014-01-28 | Butamax(Tm) Advanced Biofuels Llc | Expression of hexose kinase in recombinant host cells |
| MX2012007583A (es) | 2009-12-29 | 2012-07-30 | Butamax Tm Advanced Biofuels | Alcohol deshidrogenasas (adh) utiles para la produccion fermentativa de alcoholes alquilicos de cadena corta. |
| CN102782119B (zh) | 2010-02-17 | 2016-03-16 | 布特马斯先进生物燃料有限责任公司 | 改善需Fe-S簇蛋白质的活性 |
| WO2011149353A1 (en) * | 2010-05-27 | 2011-12-01 | C5 Yeast Company B.V. | Yeast strains engineered to produce ethanol from acetic acid and glycerol |
| AU2011268326B2 (en) | 2010-06-17 | 2015-02-05 | Butamax(Tm) Advanced Biofuels Llc | Yeast production culture for the production of butanol |
| US8871488B2 (en) | 2010-06-18 | 2014-10-28 | Butamax Advanced Biofuels Llc | Recombinant host cells comprising phosphoketolases |
| NZ707800A (en) | 2010-09-07 | 2016-09-30 | Butamax Tm Advanced Biofuels | Integration of a polynucleotide encoding a polypeptide that catalyzes pyruvate to acetolactate conversion |
| CN103502432A (zh) * | 2010-11-22 | 2014-01-08 | 诺维信股份有限公司 | 用于生产3-羟基丙酸的组合物和方法 |
| EP2689014B1 (en) | 2011-03-24 | 2019-04-24 | Butamax (TM) Advanced Biofuels LLC | Host cells and methods for production of isobutanol |
| EP3483279A1 (en) * | 2011-04-05 | 2019-05-15 | Lallemand Hungary Liquidity Management LLC | Methods for the improvement of product yield and production in a microorganism through the addition of alternate electron acceptors |
| US20120258873A1 (en) | 2011-04-06 | 2012-10-11 | Butamax(Tm) Advanced Biofuels Llc | Reduction of 2,3-dihydroxy-2-methyl butyrate (dhmb) in butanol production |
| JP2014522634A (ja) | 2011-06-17 | 2014-09-08 | ビュータマックス・アドバンスド・バイオフューエルズ・エルエルシー | イソブタノール発酵の原料としてのリグノセルロース加水分解物 |
| CN105473726B (zh) | 2011-07-28 | 2021-03-12 | 布特马斯先进生物燃料有限责任公司 | 酮异戊酸脱羧酶及其使用方法 |
| CA2857498A1 (en) | 2011-11-30 | 2013-09-26 | Mascoma Corporation | Engineering an increase in ethanol production by altering cofactor specificity |
| US9181566B2 (en) | 2011-12-30 | 2015-11-10 | Butamax Advanced Biofuels Llc | Genetic switches for butanol production |
| US9909148B2 (en) | 2011-12-30 | 2018-03-06 | Butamax Advanced Biofuels Llc | Fermentative production of alcohols |
| KR20140146616A (ko) | 2012-03-23 | 2014-12-26 | 부타맥스 어드밴스드 바이오퓨얼스 엘엘씨 | 부타놀로겐용 배지의 아세테이트 보충물 |
| MX2014013446A (es) | 2012-05-11 | 2014-12-08 | Butamax Advanced Biofuels Llc | Enzimas de cetol-acido reductoisomerasa y metodos de uso. |
| AU2013323427A1 (en) | 2012-09-26 | 2015-03-05 | Butamax (Tm) Advanced Biofuels Llc | Polypeptides with ketol-acid reductoisomerase activity |
| US9273330B2 (en) | 2012-10-03 | 2016-03-01 | Butamax Advanced Biofuels Llc | Butanol tolerance in microorganisms |
| US9988650B2 (en) | 2012-11-20 | 2018-06-05 | Lallenmend Hungary Liquidity Management LLC | Electron consuming ethanol production pathway to displace glycerol formation in S. cerevisiae |
| US20140186911A1 (en) | 2012-12-28 | 2014-07-03 | Butamax Advanced Biofuels Llc | Recombinant host cells and methods for producing butanol |
| WO2014106107A2 (en) | 2012-12-28 | 2014-07-03 | Butamax (Tm) Advanced Biofuels Llc | Dhad variants for butanol production |
| ES2735024T3 (es) | 2013-03-14 | 2019-12-13 | Du Pont | Glicerol-3-fosfato deshidrogenasa para la producción de butanol |
| US9580705B2 (en) | 2013-03-15 | 2017-02-28 | Butamax Advanced Biofuels Llc | DHAD variants and methods of screening |
-
2014
- 2014-03-13 ES ES14719570T patent/ES2735024T3/es active Active
- 2014-03-13 CA CA2905912A patent/CA2905912A1/en not_active Abandoned
- 2014-03-13 HU HUE14719570A patent/HUE045200T2/hu unknown
- 2014-03-13 US US14/208,474 patent/US9441250B2/en active Active
- 2014-03-13 JP JP2016501949A patent/JP2016511010A/ja active Pending
- 2014-03-13 AU AU2014244216A patent/AU2014244216A1/en not_active Abandoned
- 2014-03-13 WO PCT/US2014/025714 patent/WO2014160050A1/en not_active Ceased
- 2014-03-13 BR BR112015022612A patent/BR112015022612A2/pt not_active IP Right Cessation
- 2014-03-13 CN CN201480027043.7A patent/CN105378057A/zh active Pending
- 2014-03-13 EP EP14719570.5A patent/EP2970863B1/en active Active
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2015
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|---|---|
| BR112015022612A2 (pt) | 2017-10-24 |
| US20160376613A1 (en) | 2016-12-29 |
| CN105378057A (zh) | 2016-03-02 |
| WO2014160050A1 (en) | 2014-10-02 |
| EP2970863A1 (en) | 2016-01-20 |
| EP2970863B1 (en) | 2019-04-24 |
| JP2016511010A (ja) | 2016-04-14 |
| US9441250B2 (en) | 2016-09-13 |
| ZA201507360B (en) | 2016-12-21 |
| US9944954B2 (en) | 2018-04-17 |
| CA2905912A1 (en) | 2014-10-02 |
| HUE045200T2 (hu) | 2019-12-30 |
| US20140273129A1 (en) | 2014-09-18 |
| AU2014244216A1 (en) | 2015-09-24 |
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