ES2687144T3 - Métodos y composiciones para la captura y liberación altamente específicas de materiales biológicos - Google Patents

Métodos y composiciones para la captura y liberación altamente específicas de materiales biológicos Download PDF

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Publication number
ES2687144T3
ES2687144T3 ES12742728.4T ES12742728T ES2687144T3 ES 2687144 T3 ES2687144 T3 ES 2687144T3 ES 12742728 T ES12742728 T ES 12742728T ES 2687144 T3 ES2687144 T3 ES 2687144T3
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Prior art keywords
cells
cell
hydrogel
alginic acid
peg
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Active
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ES12742728.4T
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English (en)
Spanish (es)
Inventor
Shashi K. Murthy
Adam HATCH
George HANSMANN
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Northeastern University China
Northeastern University Boston
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Northeastern University China
Northeastern University Boston
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/30—Micromixers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/34—Purifying; Cleaning
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54386—Analytical elements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
    • G01N33/548—Carbohydrates, e.g. dextran
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
    • G01N33/553—Metal or metal coated
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F25/00—Flow mixers; Mixers for falling materials, e.g. solid particles
    • B01F25/40—Static mixers
    • B01F25/42—Static mixers in which the mixing is affected by moving the components jointly in changing directions, e.g. in tubes provided with baffles or obstructions
    • B01F25/43—Mixing tubes, e.g. wherein the material is moved in a radial or partly reversed direction
    • B01F25/431—Straight mixing tubes with baffles or obstructions that do not cause substantial pressure drop; Baffles therefor
    • B01F25/4317—Profiled elements, e.g. profiled blades, bars, pillars, columns or chevrons
    • B01F25/43172—Profiles, pillars, chevrons, i.e. long elements having a polygonal cross-section
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F25/00—Flow mixers; Mixers for falling materials, e.g. solid particles
    • B01F25/40—Static mixers
    • B01F25/42—Static mixers in which the mixing is affected by moving the components jointly in changing directions, e.g. in tubes provided with baffles or obstructions
    • B01F25/43—Mixing tubes, e.g. wherein the material is moved in a radial or partly reversed direction
    • B01F25/431—Straight mixing tubes with baffles or obstructions that do not cause substantial pressure drop; Baffles therefor
    • B01F25/43197—Straight mixing tubes with baffles or obstructions that do not cause substantial pressure drop; Baffles therefor characterised by the mounting of the baffles or obstructions
    • B01F25/431971—Mounted on the wall
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0636—Focussing flows, e.g. to laminate flows
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0652—Sorting or classification of particles or molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/069—Absorbents; Gels to retain a fluid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0867—Multiple inlets and one sample wells, e.g. mixing, dilution
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0478—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure pistons
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Zoology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Inorganic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Medicinal Preparation (AREA)
  • Compositions Of Macromolecular Compounds (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
ES12742728.4T 2011-02-03 2012-02-03 Métodos y composiciones para la captura y liberación altamente específicas de materiales biológicos Active ES2687144T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161439166P 2011-02-03 2011-02-03
US201161439166P 2011-02-03
PCT/US2012/023859 WO2012106658A1 (en) 2011-02-03 2012-02-03 Methods and compositions for highly specific capture and release of biological materials

Publications (1)

Publication Number Publication Date
ES2687144T3 true ES2687144T3 (es) 2018-10-23

Family

ID=46603107

Family Applications (1)

Application Number Title Priority Date Filing Date
ES12742728.4T Active ES2687144T3 (es) 2011-02-03 2012-02-03 Métodos y composiciones para la captura y liberación altamente específicas de materiales biológicos

Country Status (7)

Country Link
US (1) US9927334B2 (enExample)
EP (1) EP2670856B1 (enExample)
JP (2) JP6120778B2 (enExample)
KR (1) KR101922741B1 (enExample)
ES (1) ES2687144T3 (enExample)
SG (2) SG10201600803SA (enExample)
WO (1) WO2012106658A1 (enExample)

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WO2014072465A1 (en) 2012-11-09 2014-05-15 Roche Diagnostics Gmbh In vitro capture and analysis of circulating tumor cells
WO2014121204A1 (en) * 2013-02-01 2014-08-07 The General Hospital Corporation Capture and release of particles from liquid samples
US20170074870A1 (en) 2014-03-14 2017-03-16 Northeastern University Microfluidic System and Method for Real-Time Measurement of Antibody-Antigen Binding and Analyte Detection
WO2015148512A1 (en) 2014-03-24 2015-10-01 Qt Holdings Corp Shaped articles including hydrogels and methods of manufacture and use thereof
PT3237597T (pt) * 2014-12-22 2021-03-12 Ecole Polytechnique Fed Lausanne Epfl Dispositivos para agregação de alto rendimento e manipulação de células de mamífero
US9790467B2 (en) * 2015-09-22 2017-10-17 Qt Holdings Corp Methods and compositions for activation or expansion of T lymphocytes
ES2871137T3 (es) * 2015-12-14 2021-10-28 Quanta Matrix Co Ltd Método de diferenciación de células particulares a partir de la mezcla de células heterogéneas
SG11201808014SA (en) 2016-03-18 2018-10-30 Qt Holdings Corp Compositions, devices, and methods for cell separation
US10429387B2 (en) * 2016-07-27 2019-10-01 Universiteit Tweate Simple and affordable method for immuophenotyping using a microfluidic chip sample preparation with image cytometry
US11118163B2 (en) 2017-02-02 2021-09-14 Sartorius Stedim Biotech Gmbh Separation of cell populations by marker identification and sedimentation velocity
DE102017105195A1 (de) 2017-03-10 2018-09-13 Leibniz-Institut Für Polymerforschung Dresden E.V. Verfahren zur differenzierten Sequestrierung von Stoffen verschiedener Stoffgruppen mit Hilfe von sulfatierte oder sulfonierte Komponenten enthaltenden Hydrogelen
US20200085971A1 (en) * 2017-03-20 2020-03-19 Qt Holdings Corp Methods and compositions for modulation of immune cells
WO2019239359A2 (en) 2018-06-13 2019-12-19 University Of Washington Extruded hydrogel tubes and coaxial fibers and applications thereof
DK3626814T3 (da) * 2018-09-21 2023-06-06 Univ Wien Tech Fremstilling af cellesfæroider
JP7120547B2 (ja) * 2019-02-22 2022-08-17 国立研究開発法人理化学研究所 物質固定化剤、及び当該物質固定化剤を用いた物質固定化方法
EP3941630B1 (en) * 2019-03-18 2025-04-16 Cellular Research, Inc. Precise delivery of components into fluids
US11668676B2 (en) * 2019-05-30 2023-06-06 The Regents Of The University Of California Microfluidic acoustic devices and methods
CN112708150B (zh) * 2020-12-08 2021-11-16 济南国科医工科技发展有限公司 用于循环肿瘤细胞的捕获及定点释放的水凝胶体系
KR20240051155A (ko) 2021-09-01 2024-04-19 바이오-테크네 코포레이션 인간 비트로넥틴 단편 및 이의 용도
US11805995B1 (en) 2022-11-22 2023-11-07 King Faisal University Saliva collection kit

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AU733716B2 (en) * 1996-09-19 2001-05-24 Regents Of The University Of Michigan, The Polymers containing polysaccharides such as alginates or modified alginates
US20050065281A1 (en) 2001-11-07 2005-03-24 Mathias Lutolf Synthetic matrix for controlled cell ingrowth and tissue regeneration
ES2301697T3 (es) * 2001-12-18 2008-07-01 Eidgenossisch Technische Hochschule Zurich Matrices de proteina modificada con factor de crecimiento para ingenieria de tejidos.
WO2004008142A1 (ja) * 2002-07-12 2004-01-22 Mitsubishi Chemical Corporation 分析用チップ、分析用チップユニット、分析装置及びそれを用いた分析方法並びに分析用チップの作製方法
EP1569510B1 (en) * 2002-09-27 2011-11-02 The General Hospital Corporation Microfluidic device for cell separation and uses thereof
TW200605910A (en) 2004-04-30 2006-02-16 Orbus Medical Technologies Inc Medical device with coating for capturing genetically-altered cells and methods for using same
SE0402476D0 (sv) * 2004-10-13 2004-10-13 Biacore Ab Preparation and use of a reactive solid support surface
CN101338036B (zh) * 2007-07-06 2010-11-03 常州百瑞吉生物医药有限公司 生物相容快速凝胶化水凝胶及其喷雾剂的制备方法
US9267109B2 (en) * 2007-12-10 2016-02-23 Koninklijke Philips N.V. Patterned cell sheets and a method for production of the same
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US20140154703A1 (en) * 2011-01-06 2014-06-05 Alison Skelley Circulating tumor cell capture on a microfluidic chip incorporating both affinity and size

Also Published As

Publication number Publication date
EP2670856A1 (en) 2013-12-11
JP2014506671A (ja) 2014-03-17
JP2017077245A (ja) 2017-04-27
SG192587A1 (en) 2013-09-30
EP2670856A4 (en) 2014-12-03
EP2670856B1 (en) 2018-06-27
US9927334B2 (en) 2018-03-27
JP6120778B2 (ja) 2017-04-26
WO2012106658A1 (en) 2012-08-09
SG10201600803SA (en) 2016-03-30
KR20140016896A (ko) 2014-02-10
US20140057280A1 (en) 2014-02-27
KR101922741B1 (ko) 2019-02-20

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