ES2662885T3 - Método de cultivo de células tenocitos - Google Patents
Método de cultivo de células tenocitos Download PDFInfo
- Publication number
- ES2662885T3 ES2662885T3 ES07718609.6T ES07718609T ES2662885T3 ES 2662885 T3 ES2662885 T3 ES 2662885T3 ES 07718609 T ES07718609 T ES 07718609T ES 2662885 T3 ES2662885 T3 ES 2662885T3
- Authority
- ES
- Spain
- Prior art keywords
- cell culture
- culture method
- tenocyte cell
- tenocyte
- approximately
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/066—Tenocytes; Tendons, Ligaments
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P19/00—Drugs for skeletal disorders
- A61P19/04—Drugs for skeletal disorders for non-specific disorders of the connective tissue
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/30—Hormones
- C12N2501/33—Insulin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/30—Hormones
- C12N2501/38—Hormones with nuclear receptors
- C12N2501/39—Steroid hormones
Abstract
Un medio de cultivo de tenocitos que comprende aproximadamente 0,0006 % en p/v de insulina y aproximadamente 0,0002 % en p/v de betametasona, en el que dicho medio tiene la capacidad de expandir selectivamente tenocitos en cultivo.
Description
Para producir un tamaño de producto de 254 pb.
Gliceraldehído-3-fosfato deshidrogenasa humana (GAPDH)
5 Sentido 5'TCACCATCTTCCAGGAGCGA 3' (SEQ ID NO: 5)
Antisentido 5'CACAATGCCGAAGTGGTCGT 3' (SEQ ID NO: 6) 10 Para producir un tamaño de producto de 293 pb.
Se realizaron 30 ciclos de PCR en un termociclador en condiciones estándar, excepto que se utilizó un tiempo de recocido de 40 segundos y la hibridación usando los cebadores de colágeno de tipo I y III se llevó a cabo a 60 ºC y 15 los cebadores de GAPDH a 62 ºC.
Las reacciones de PCR se analizaron por electroforesis y los resultados se muestran en la Figura 1. Se puede observar que los tenocitos cultivados en un medio de la invención expresan ARNm de colágeno de tipo I y tipo III, al igual que el tejido de tendón.
20
11
Claims (1)
-
imagen1
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AU2006901495 | 2006-03-23 | ||
AU2006901495A AU2006901495A0 (en) | 2006-03-23 | Cell culture | |
PCT/AU2007/000362 WO2007106949A1 (en) | 2006-03-23 | 2007-03-23 | Tenocyte cell culturing method |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2662885T3 true ES2662885T3 (es) | 2018-04-10 |
Family
ID=38521955
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES07718609.6T Active ES2662885T3 (es) | 2006-03-23 | 2007-03-23 | Método de cultivo de células tenocitos |
Country Status (9)
Country | Link |
---|---|
US (1) | US7985408B2 (es) |
EP (1) | EP2004805B1 (es) |
CN (2) | CN101448933A (es) |
AU (1) | AU2007229273B2 (es) |
CA (1) | CA2641063C (es) |
ES (1) | ES2662885T3 (es) |
HK (1) | HK1179299A1 (es) |
NZ (1) | NZ571913A (es) |
WO (1) | WO2007106949A1 (es) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101795718B (zh) * | 2007-04-24 | 2013-06-12 | 西澳大利亚大学 | 含肌腱细胞的生物支架和使用该生物支架的治疗方法 |
US20120301507A1 (en) * | 2009-03-27 | 2012-11-29 | Orthocell Pty Ltd | Method of tissue repair |
CN110499282B (zh) * | 2019-09-03 | 2021-05-11 | 广州赛莱拉干细胞科技股份有限公司 | 一种培养基及其应用和诱导肌腱干细胞向脂肪细胞分化的方法 |
CN111778207A (zh) * | 2020-06-17 | 2020-10-16 | 北京大学口腔医学院 | 一种三维TSPCs球体肌腱向分化诱导培养基及分化培养方法 |
Family Cites Families (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2594123B2 (ja) * | 1987-09-12 | 1997-03-26 | 株式会社林原生物化学研究所 | 減感作剤 |
US20020173806A1 (en) * | 1996-08-30 | 2002-11-21 | Verigen Transplantation Service International (Vtsi) Ag | Method for autologous transplantation |
US6569172B2 (en) * | 1996-08-30 | 2003-05-27 | Verigen Transplantation Service International (Vtsi) | Method, instruments, and kit for autologous transplantation |
IT1292410B1 (it) * | 1997-06-24 | 1999-02-08 | Roberto Beretta | Contenitore di pronto impiego per ottenere colla di fibrina autologa |
FR2799371A1 (fr) * | 1999-10-12 | 2001-04-13 | Univ Paris Curie | Compositions de tenocytes preparation et utilisations |
KR20040053137A (ko) * | 2001-09-24 | 2004-06-23 | 베리겐 아게 | 자가 성장 인자 칵테일 조성물, 이의 제조 방법 및 용도 |
AU2003245251A1 (en) | 2002-05-01 | 2003-11-17 | Verigen Ag | Injectable chondrocyte implant |
US7824701B2 (en) | 2002-10-18 | 2010-11-02 | Ethicon, Inc. | Biocompatible scaffold for ligament or tendon repair |
US8226715B2 (en) | 2003-06-30 | 2012-07-24 | Depuy Mitek, Inc. | Scaffold for connective tissue repair |
US8043614B2 (en) * | 2004-03-09 | 2011-10-25 | Ahlfors Jan-Eric W | Autogenic living scaffolds and living tissue matrices: methods and uses thereof |
US11395865B2 (en) * | 2004-02-09 | 2022-07-26 | DePuy Synthes Products, Inc. | Scaffolds with viable tissue |
-
2007
- 2007-03-23 NZ NZ571913A patent/NZ571913A/en unknown
- 2007-03-23 CN CNA2007800184408A patent/CN101448933A/zh active Pending
- 2007-03-23 ES ES07718609.6T patent/ES2662885T3/es active Active
- 2007-03-23 CA CA2641063A patent/CA2641063C/en active Active
- 2007-03-23 WO PCT/AU2007/000362 patent/WO2007106949A1/en active Application Filing
- 2007-03-23 US US12/294,169 patent/US7985408B2/en active Active
- 2007-03-23 EP EP07718609.6A patent/EP2004805B1/en active Active
- 2007-03-23 CN CN201210135419.9A patent/CN102876627B/zh active Active
- 2007-03-23 AU AU2007229273A patent/AU2007229273B2/en active Active
-
2013
- 2013-05-24 HK HK13106135.8A patent/HK1179299A1/xx unknown
Also Published As
Publication number | Publication date |
---|---|
US7985408B2 (en) | 2011-07-26 |
CN102876627A (zh) | 2013-01-16 |
CN102876627B (zh) | 2014-10-22 |
NZ571913A (en) | 2011-11-25 |
US20090191161A1 (en) | 2009-07-30 |
EP2004805A1 (en) | 2008-12-24 |
HK1179299A1 (en) | 2013-09-27 |
EP2004805A4 (en) | 2009-03-25 |
AU2007229273A1 (en) | 2007-09-27 |
EP2004805B1 (en) | 2018-01-24 |
CN101448933A (zh) | 2009-06-03 |
CA2641063C (en) | 2017-11-28 |
CA2641063A1 (en) | 2007-09-27 |
WO2007106949A1 (en) | 2007-09-27 |
AU2007229273B2 (en) | 2011-03-10 |
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