ES2566977B1 - Food product comprising xylose, p-cimenol and protease - Google Patents
Food product comprising xylose, p-cimenol and protease Download PDFInfo
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
Abstract
Producto alimenticio que comprende xilosa, p-cimenol y proteasa.#La presente invención se refiere a un producto alimenticio que comprende xilosa, p-cimenol y proteasa, así como a su procedimiento de obtención y su uso en la alimentación de animales.Food product comprising xylose, p-cimenol and protease. # The present invention relates to a food product comprising xylose, p-cimenol and protease, as well as its method of obtaining and its use in animal feeding.
Description
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DESCRIPCIONDESCRIPTION
PRODUCTO ALIMENTICIO QUE COMPRENDE XILOSA, P-CIMENOL Y PROTEASA Campo de la invencionFOOD PRODUCT THAT INCLUDES XYLOSA, P-CIMENOL AND PROTEASA Field of the Invention
La presente invencion se refiere al campo de la alimentacion en animales. En particular, la presente invencion se refiere a una nueva composition alimenticia que comprende xilosa, p- cimenol y proteasa, as^ como su fabrication y utilization para animales.The present invention relates to the field of animal feeding. In particular, the present invention relates to a novel food composition comprising xylose, pimenimen and protease, as well as its manufacture and utilization for animals.
Antecedentes de la invencionBackground of the invention
Es conocido que en la alimentacion de animales llegan al intestino elevadas cantidades de protemas no digeridas, las cuales al no estar digeridas no pueden ser absorbidas y, por otro lado, son aprovechadas por bacterias patogenas, como Clostridium, que si las metabolizan. Esto provoca que aumenten las poblaciones de Clostridium, reduciendo la poblacion de biota propia del intestino. Por consiguiente, el intestino funcionara de una manera no correcta, e incluso en algunos casos pueden llegar a producirse diarreas.It is known that in the feeding of animals high amounts of undigested proteins reach the intestine, which, when not digested, cannot be absorbed and, on the other hand, are used by pathogenic bacteria, such as Clostridium, which do metabolize them. This causes Clostridium populations to increase, reducing the population of the intestine's own biota. Therefore, the intestine will function in an incorrect way, and even in some cases diarrhea can occur.
La presente invencion proporciona un producto novedoso con un contenido en sus principios activos que es superior al descrito en la literatura y comprende una serie de propiedades que satisfacen la necesidad de un producto alimenticio que ayude a la digestion de alimentos en animales.The present invention provides a novel product with a content in its active ingredients that is superior to that described in the literature and comprises a series of properties that satisfy the need for a food product that helps digestion of food in animals.
Por un lado, este producto novedoso se presenta en un perfecto estado de conservation, sin presentar sustancias nocivas y con todas sus propiedades nutricionales. Funcionalmente, este producto consigue la mejora de la digestion de las protemas y por lo tanto su absorcion y aprovechamiento por parte del animal y evita el crecimiento de bacterias patogenas que compiten por el espacio intestinal con las bacterias beneficiosas para el organismo del animal. Adicionalmente, se mejora la microflora intestinal que ayudara a un mejor funcionamiento intestinal y por tanto a una mejor absorcion de los nutrientes de la dieta.On the one hand, this novel product is presented in a perfect state of conservation, without presenting harmful substances and with all its nutritional properties. Functionally, this product achieves the improvement of protein digestion and therefore its absorption and use by the animal and prevents the growth of pathogenic bacteria that compete for the intestinal space with the beneficial bacteria for the animal's organism. Additionally, intestinal microflora is improved that will help better intestinal functioning and therefore better absorption of nutrients from the diet.
Asi mismo, la asociacion de los tres principios activos de la presente invencion (xilosa, p- cimenol y proteasa) mejora sorprendentemente la productividad de las explotaciones ya que se mejora el mdice de conversion de los animales (relation existente ente el peso ganado por el animal y el alimento consumido) al mejorarse su bienestar digestivo.Likewise, the association of the three active principles of the present invention (xylose, pimenimen and protease) surprisingly improves the productivity of the farms since the conversion rate of the animals is improved (relation existing between the weight gained by the animal and food consumed) to improve their digestive well-being.
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Breve descripcion de los dibujosBrief description of the drawings
Figura 1: Molecula de xilosa Figura 2: Molecula de p-cimenol Figura 3: Proteasa Descripcion de la invencionFigure 1: Molecule of xylose Figure 2: Molecula of p-cimenol Figure 3: Protease Description of the invention
En un primer aspecto, la presente invencion se refiere a un producto alimenticio que comprende xilosa, p-cimenol y proteasa.In a first aspect, the present invention relates to a food product comprising xylose, p-cimenol and protease.
En una realizacion preferida, en el producto alimenticio la xilosa esta presente en como mmimo el 2,25% en peso en dicho producto.In a preferred embodiment, in the food product the xylose is present in at least 2.25% by weight in said product.
En otra realizacion preferida, en el producto alimenticio el p-cimenol esta presente en como mmimo el 3,5% en peso en dicho producto.In another preferred embodiment, in the food product the p-cimenol is present in at least 3.5% by weight in said product.
En aun otra realizacion preferida, en el producto alimenticio la proteasa esta presente en como mmimo el 5,5% en peso en dicho producto.In yet another preferred embodiment, in the food product the protease is present in at least 5.5% by weight in said product.
En otra realizacion adicional de la presente invencion, la xilosa del producto alimenticio esta contenida originalmente en harina de Ceratonia siliqua. Preferiblemente, la xilosa esta presente en la harina de Ceratonia siliqua en un porcentaje en peso entre el 2 y el 4%.In another additional embodiment of the present invention, the xylose of the food product is originally contained in Ceratonia siliqua flour. Preferably, xylose is present in Ceratonia siliqua flour in a weight percentage between 2 and 4%.
En otra realizacion adicional de la presente invencion, el p-cimenol del producto alimenticio esta contenido originalmente en un extracto de Thymus vulgaris. Preferiblemente, el p- cimenol esta presente en dicho extracto en un porcentaje en peso entre el 15 y el 17%.In a further embodiment of the present invention, the p-cimenol of the food product is originally contained in an extract of Thymus vulgaris. Preferably, the pimenimen is present in said extract in a weight percentage between 15 and 17%.
En otra realizacion adicional de la presente invencion, la proteasa del producto alimenticio esta contenido originalmente en un extracto de Cynara cardunculus. Preferiblemente, la proteasa esta presente en dicho extracto en un porcentaje en peso entre el 20 y el 26%.In a further embodiment of the present invention, the protease of the food product is originally contained in an extract of Cynara cardunculus. Preferably, the protease is present in said extract in a weight percentage between 20 and 26%.
En una realizacion mas preferida de la proteasa mencionada en la presente invencion, la proteasa es cynarasa.In a more preferred embodiment of the protease mentioned in the present invention, the protease is cynarase.
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En un segundo aspecto, la presente invention se refiere a un procedimiento para la obtencion de un producto alimenticio que comprende xilosa, p-cimenol y proteasa, que comprende las etapas de:In a second aspect, the present invention relates to a process for obtaining a food product comprising xylose, p-cimenol and protease, comprising the steps of:
(a) obtener un extracto de proteasas de la flor de Cynara cardunculus;(a) obtain a protease extract from the flower of Cynara cardunculus;
(b) obtener un extracto de p-cimenol de las hojas de Thymus vulgaris;(b) obtain an extract of p-cimenol from the leaves of Thymus vulgaris;
(c) mezclar los extractos de las etapas (a) y (b) con harina de Ceratonia siliquia hasta homogeneidad.(c) mix the extracts from stages (a) and (b) with Ceratonia siliquia flour until homogeneous.
En una realization preferida del procedimiento de la presente invencion, la proteasa en la etapa (a) es cynarasa. Cabe indicar que existen diversas proteasas presentes en la flor de Cynara cardunculus: las cynarasas y las cardosinas. Las primeras se obtienen mediante el extracto acuoso a pH 8,3 de las flores senescentes desecadas al aire y las cardosinas se obtienen de los estigmas frescos por extraction a pH 3. La presente invencion se centra preferiblemente en la extraccion de cynarasa por su estabilidad durante el proceso de purification, asi como su mayor capacidad proteolrtica, caracteristica de gran interes para su adicion al pienso complementario.In a preferred embodiment of the process of the present invention, the protease in step (a) is cynarase. It should be noted that there are several proteases present in the flower of Cynara cardunculus: cynarasas and cardosinas. The former are obtained by means of the aqueous extract at pH 8.3 of the senescent flowers dried in the air and the cardosins are obtained from the fresh stigmas by extraction at pH 3. The present invention preferably focuses on the extraction of cynarase because of its stability during the purification process, as well as its greater proteolytic capacity, characteristic of great interest for its addition to complementary feed.
En particular, en otra realizacion preferida del procedimiento de la presente invencion, la etapa (a) comprende las siguientes subetapas:In particular, in another preferred embodiment of the process of the present invention, step (a) comprises the following sub-stages:
(a1) macerar las flores secas de Cynara cardunculus en agua acidificada con un acido fuerte hasta pH 4;(a1) macerate the dried flowers of Cynara cardunculus in acidified water with a strong acid until pH 4;
(a2) filtrar la mezcla guardando la fase acuosa;(a2) filter the mixture keeping the aqueous phase;
(a3) utilizar una bentonita para absorber la fase acuosa y obtener el extracto en polvo.(a3) use a bentonite to absorb the aqueous phase and obtain the powder extract.
Sorprendentemente, mediante la acidification se consigue aumentar el rendimiento de la extraccion. Esta acidificacion del medio permite mejorar el rendimiento de la solution enzimatica por desplazamiento de los enzimas proteolrticos que son acidos debiles, por un acido fuerte.Surprisingly, through acidification it is possible to increase the extraction yield. This acidification of the medium allows to improve the performance of the enzymatic solution by displacement of proteolytic enzymes that are weak acids, by a strong acid.
En otra realizacion preferida del procedimiento de la presente invencion, la etapa (b) comprende las siguientes subetapas:In another preferred embodiment of the method of the present invention, step (b) comprises the following sub-stages:
(b1) suspender en agua las hojas de Thymus vulgaris;(b1) suspend the leaves of Thymus vulgaris in water;
(b2) destilar el aceite del extracto de las hojas;(b2) distill the oil from the leaf extract;
(b3) extraer el aceite de la fase acuosa;(b3) extract the oil from the aqueous phase;
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(b4) extraer con una base el contenido fenolico del aceite y solubilizarlo en agua;(b4) extract with a base the phenolic content of the oil and solubilize it in water;
(b5) extraer el p-cimenol con eter;(b5) extract the p-cimenol with ether;
(b6) evaporar el eter;(b6) evaporate the ether;
(b7) utilizar una bentonita para absorber la fase liquida y obtener el extracto en polvo.(b7) use a bentonite to absorb the liquid phase and obtain the powder extract.
En un tercer aspecto, la presente invention se refiere a la utilization de un producto alimenticio en la alimentation de animales. Este producto alimenticio mejora el funcionamiento intestinal de los animales debido a su action en la mejor digestion de las protemas, mejora de la flora intestinal y permite que el alimento llegue al momento del consumo final con todas sus propiedades nutricionales y organolepticas intactas.In a third aspect, the present invention relates to the use of a food product in animal feed. This food product improves the intestinal functioning of the animals due to its action in the best digestion of the proteins, improves the intestinal flora and allows the food to reach the moment of final consumption with all its intact nutritional and organoleptic properties.
La presente invencion se ilustra adicionalmente mediante referencia a los siguientes ejemplos sin que los mismos representen una limitation del alcance de la presente invencion.The present invention is further illustrated by reference to the following examples without representing a limitation of the scope of the present invention.
EJEMPLOSEXAMPLES
Ejemplo 1. Obtencion de proteasasExample 1. Obtaining Proteases
Una vez las flores de Cynara cardunculus se han recolectado, se dejan secar al aire. Posteriormente se realiza una maceration de las flores secas en agua a temperatura ambiente, de manera que las cynarasas son transferidas al medio acuoso, acidificado a pH 4 mediante un acido fuerte, en concreto HCl, por ser altamente solubles. Esta maceracion se lleva a cabo durante 24 horas, aunque si la temperatura ambiente es menor de 18 °C, este periodo se alarga hasta 36 horas, en una relation peso/ volumen de 1/5. Pasado el periodo transcurrido, la mezcla se filtra y se mantiene la fase acuosa. Esta fase acuosa es absorbida por una bentonita capa de absorber tres veces su peso, de manera que se obtiene finalmente el polvo deseado para mezclar con la harina de Ceratonia siliqua.Once the flowers of Cynara cardunculus have been collected, they are allowed to air dry. Subsequently, a maceration of the dried flowers is carried out in water at room temperature, so that the cynarases are transferred to the aqueous medium, acidified to pH 4 by means of a strong acid, in particular HCl, for being highly soluble. This maceration is carried out for 24 hours, although if the ambient temperature is less than 18 ° C, this period is extended up to 36 hours, in a weight / volume ratio of 1/5. After the elapsed period, the mixture is filtered and the aqueous phase is maintained. This aqueous phase is absorbed by a bentonite layer to absorb three times its weight, so that the desired powder is finally obtained for mixing with Ceratonia siliqua flour.
Una vez este obtenido, se lleva a cabo la estandarizacion del mismo para comprobar que la concentration de enzimas proteolrticos es de entre 22 y 24%.Once this is obtained, the standardization of the same is carried out to verify that the concentration of proteolytic enzymes is between 22 and 24%.
Ejemplo 2: Obtencion del p-cimenol (tambien referido aqui como simplemente cimenol)Example 2: Obtaining p-cimenol (also referred to here as simply cimenol)
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Para la obtencion de cimenol, se coloca el tomillo seco y molido en un balon con agua suficiente para lograr la suspension de la muestra. Esto suele ser una proportion de 1 kilo de tomillo seco en 3 de agua. Se busca la menor manipulation posible de la muestra con el objetivo de no degradar los aceites esenciales que son la fuente principal del compuesto a purificar. Por ello, para la extraction del aceite esencial de tomillo se usa una destilacion con arrastre de vapor. Debido a la volatilidad y la insolubilidad del aceite en agua se esperara que el producto presente dos fases. Posteriormente se hace una extraccion para separar el aceite esencial puro con un embudo de separation. Se separa el aceite esencial en un recipiente tapado y protegido de la luz para evitar la descomposicion lummica. Con la parte acuosa se realiza una nueva extraccion con eter para lograr asi separar finalmente los excedentes de aceite que queden solubilizados en esta fase. A la fase eterea se le extraen las trazas de agua remanente con sulfato de sodio como desecante. Para reducir la cantidad de componentes a separar mediante cromatograria se realiza una extraccion acido-base. Para la obtencion de los fenoles, entre los cuales el cimenol, que son los compuestos de mayor acidez en el aceite esencial, se hace una extraccion acido-base. En primer lugar se coloca en el embudo de separacion los aceites obtenidos junto con agua destilada. A continuation, se agregan 50 ml hidroxido de potasio 1N haciendo que los fenoles, por ser los compuestos mas acidos presentes en aceite, sean solubles en la fase acuosa. Una vez aislada la fase acuosa del resto se la lleva a un nuevo embudo de separacion para su acidification con 15 ml acido clorhidrico 2N y se agregan 100 ml de eter. De esta manera se obtienen solamente los fenoles en fase eterea. Se juntan todas las fases etereas y se filtran con sulfato de sodio anhidro como desecante para eliminar las trazas de agua. Posteriormente se llevo el compuesto a calentar en bano maria para evaporar el eter y obtener asi los fenoles aislados. Al igual que con la proteasa este aceite es absorbido con una bentonita, lo que permite tener el producto en un polvo fino con un 14-16 % de cimenol. Se lleva a cabo una estandarizacion del extracto para comprobar la pureza y riqueza del mismo.To obtain cimenol, the dried and ground thyme is placed in a balloon with enough water to achieve the suspension of the sample. This is usually a proportion of 1 kilo of dried thyme in 3 of water. The smallest possible manipulation of the sample is sought in order not to degrade the essential oils that are the main source of the compound to be purified. Therefore, a distillation with steam entrainment is used for the extraction of thyme essential oil. Due to the volatility and insolubility of the oil in water, the product will be expected to present two phases. Subsequently, an extraction is made to separate the pure essential oil with a separating funnel. The essential oil is separated in a covered container and protected from light to prevent light decomposition. With the aqueous part, a new extraction with ether is carried out in order to finally separate the excess oil that is solubilized in this phase. The traces of water remaining with sodium sulfate as a desiccant are extracted to the etheric phase. To reduce the amount of components to be separated by chromatography, an acid-base extraction is performed. To obtain the phenols, among which cimenol, which are the most acidic compounds in the essential oil, an acid-base extraction is made. First of all, the oils obtained together with distilled water are placed in the separating funnel. Next, 50 ml of 1N potassium hydroxide are added making the phenols, as the most acidic compounds present in oil, are soluble in the aqueous phase. Once the aqueous phase is isolated from the rest, it is taken to a new separating funnel for acidification with 15 ml 2N hydrochloric acid and 100 ml of ether are added. In this way only phenols in the etheric phase are obtained. All the etheric phases are combined and filtered with anhydrous sodium sulfate as a desiccant to remove traces of water. Subsequently, the compound to be heated in a water bath was taken to evaporate the ether and thus obtain the isolated phenols. As with the protease this oil is absorbed with a bentonite, which allows to have the product in a fine powder with 14-16% of cimenol. A standardization of the extract is carried out to verify its purity and richness.
Ejemplo 3: Obtencion del producto alimenticio de la inventionExample 3: Obtaining the food product of the invention
Se llevan a cabo las adquisiciones de los materiales de partida: harina de Ceratonia siliqua (a la que no se realiza ningun procesamiento ya que el contenido final de xilosa sera el contenido en esta harina), Cynara cardunculus y Thymus vulgaris, en cantidad suficiente para la realization de un lote de 100 kg de alimento complementario.Acquisitions of the starting materials are carried out: Ceratonia siliqua flour (to which no processing is performed since the final content of xylose will be the content in this flour), Cynara cardunculus and Thymus vulgaris, in sufficient quantity to the realization of a batch of 100 kg of complementary food.
En primer lugar se materializan las extracciones de los principios activos de Thymus vulgaris (cimenol) y Cynara cardunculus (proteasa), tal como se ha explicado en los ejemplos 1 y 2.First, the extracts of the active substances of Thymus vulgaris (cimenol) and Cynara cardunculus (protease) materialize, as explained in examples 1 and 2.
Una vez obtenidos los tres polvos con sus respectivos principios activos, se realiza el 5 analisis de cada uno de ellos para la comprobacion de la riqueza y se procede a la mezcla en un reactor de acero inoxidable con agitacion de 25 r.p.m.Once the three powders with their respective active ingredients are obtained, the analysis of each of them is carried out for the verification of the wealth and the mixing is carried out in a stainless steel reactor with agitation of 25 rpm.
En este reactor se ponen 75 kilos de harina de Ceratonia siliquia, 25 kilos de harina de Cynara cardunculus y 25 kilos de harina de Thymus vulgaris. Se mezcla durante 6 minutos, 10 momento en que se puede asegurar la homogeneidad del producto. En este momento se realiza una molturacion del producto, para garantizar una granulometria apropiada pera el uso en instalaciones industriales, que seran los usuarios finales del producto. El alimento complementario obtenido por la incorporation de ingredientes naturales de plantas aromaticas a la harina de Ceratonia siliqua, esta listo para ser consumido con una 15 composition minima garantizada de 2.25 % de xilosa, 3.5 % de cimenol y 5.5 % deIn this reactor, 75 kilos of Ceratonia siliquia flour, 25 kilos of Cynara cardunculus flour and 25 kilos of Thymus vulgaris flour are placed. It is mixed for 6 minutes, at which time the homogeneity of the product can be ensured. At this time a grinding of the product is carried out, to ensure proper granulometry for use in industrial facilities, which will be the end users of the product. The complementary food obtained by the incorporation of natural ingredients of aromatic plants to Ceratonia siliqua flour, is ready to be consumed with a guaranteed minimum composition of 2.25% xylose, 3.5% cimenol and 5.5% of
proteasa. El producto final se envasa adecuadamente.protease The final product is packaged properly.
Claims (16)
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| Application Number | Priority Date | Filing Date | Title |
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| ES201431527A ES2566977B1 (en) | 2014-10-16 | 2014-10-16 | Food product comprising xylose, p-cimenol and protease |
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| Application Number | Priority Date | Filing Date | Title |
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| ES201431527A ES2566977B1 (en) | 2014-10-16 | 2014-10-16 | Food product comprising xylose, p-cimenol and protease |
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| ES2566977A1 ES2566977A1 (en) | 2016-04-18 |
| ES2566977B1 true ES2566977B1 (en) | 2017-02-13 |
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| ES201431527A Active ES2566977B1 (en) | 2014-10-16 | 2014-10-16 | Food product comprising xylose, p-cimenol and protease |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| FR2764170B1 (en) * | 1997-06-06 | 1999-08-20 | Jean Julia | ENERGY FEED FOR LIVESTOCK |
| BR9906024A (en) * | 1999-12-30 | 2001-09-18 | Julia Jean | Energetic feed for livestock, and process for producing energetic feed for livestock |
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