ES2564099T3 - Tobera para la alineación de partículas en la corriente de líquido - Google Patents

Tobera para la alineación de partículas en la corriente de líquido Download PDF

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Publication number
ES2564099T3
ES2564099T3 ES12167890.8T ES12167890T ES2564099T3 ES 2564099 T3 ES2564099 T3 ES 2564099T3 ES 12167890 T ES12167890 T ES 12167890T ES 2564099 T3 ES2564099 T3 ES 2564099T3
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nozzle
mouth
section
feed tube
dimension
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ES12167890.8T
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English (en)
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ES2564099T5 (es
Inventor
Detlef Rath
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Masterrind GmbH
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Masterrind GmbH
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=46085454&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES2564099(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Masterrind GmbH filed Critical Masterrind GmbH
Publication of ES2564099T3 publication Critical patent/ES2564099T3/es
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1404—Handling flow, e.g. hydrodynamic focusing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0608—Germ cells
    • C12N5/0612—Germ cells sorting of gametes, e.g. according to sex or motility
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/149—Optical investigation techniques, e.g. flow cytometry specially adapted for sorting particles, e.g. by their size or optical properties
    • G01N15/1492—Optical investigation techniques, e.g. flow cytometry specially adapted for sorting particles, e.g. by their size or optical properties within droplets
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1404—Handling flow, e.g. hydrodynamic focusing
    • G01N2015/1413—Hydrodynamic focussing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1481—Optical analysis of particles within droplets

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Dispersion Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Nozzles (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Infusion, Injection, And Reservoir Apparatuses (AREA)
  • Supply Of Fluid Materials To The Packaging Location (AREA)

Abstract

Tobera para la alineación de partículas para un citómetro de flujo con un primer extremo (10) y un segundo extremo (11) enfrentado y un tubito de alimentación (5) para líquido de corriente de núcleo, con su boca (12) dispuesta en el volumen interior de la tobera, que está dispuesto en una sección adyacente a su boca (12) en un eje común con la tobera, presentando el tubito de alimentación (5) una primera abertura de entrada (1) para líquido de corriente de núcleo y su boca (12) está distanciad con respecto a una abertura de salida (7) dispuesta en el segundo extremo (11) de la tobera, y la tobera presenta una segunda abertura de entrada (4) para líquido de corriente envolvente, extendiéndose la sección adyacente a la boca del tubito de alimentación (5) por la longitud completa del tubito de alimentación (5) que está dispuesta dentro de la tobera, y siendo la sección transversal interna (5A) del tubito de alimentación (5) para líquido de corriente de núcleo perpendicular al eje longitudinal del tubito de alimentación (5), caracterizada por que la sección transversal interna (5A) del tubito de alimentación (5) al menos en la sección adyacente a su boca (12), en una primera dimensión, es más pequeña al menos en el factor 0,3 que en una segunda dimensión, y por que la sección transversal interna del tubito de alimentación (5) es rectangular o 15 elíptica, y en la primera dimensión asciende de 50 μm a 200 μm, y en la segunda dimensión de 75 μm a 300 μm.

Description

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Claims (1)

  1. imagen1
    imagen2
ES12167890T 2011-05-12 2012-05-14 Tobera para la alineación de partículas en la corriente de líquido Active ES2564099T5 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102011075711A DE102011075711A1 (de) 2011-05-12 2011-05-12 Düse zur Partikelausrichtung im Flüssigkeitsstrom
DE102011075711 2011-05-12

Publications (2)

Publication Number Publication Date
ES2564099T3 true ES2564099T3 (es) 2016-03-17
ES2564099T5 ES2564099T5 (es) 2022-06-17

Family

ID=46085454

Family Applications (1)

Application Number Title Priority Date Filing Date
ES12167890T Active ES2564099T5 (es) 2011-05-12 2012-05-14 Tobera para la alineación de partículas en la corriente de líquido

Country Status (6)

Country Link
EP (1) EP2522983B2 (es)
DE (1) DE102011075711A1 (es)
DK (1) DK2522983T4 (es)
ES (1) ES2564099T5 (es)
HU (1) HUE027402T2 (es)
PL (1) PL2522983T5 (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102013208584A1 (de) * 2013-05-08 2014-11-13 Masterrind Gmbh Düse und Verfahren für die Durchflusszytometrie
CN106370564B (zh) * 2016-10-08 2023-04-14 苏州曼德克光电有限公司 一种粉尘测试光路元件的射流保护装置

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63262565A (ja) * 1987-04-20 1988-10-28 Hitachi Ltd フロ−セル
JPH02118852A (ja) 1988-10-28 1990-05-07 Nec Eng Ltd 高級言語のデバッグの簡易化方式
ATE142788T1 (de) 1989-05-10 1996-09-15 Us Agriculture Verfahren zur vorwahl des geschlechts der nachkommenschaft
US5125759A (en) 1991-02-04 1992-06-30 Chun Wendell H Adjustable compliant robotic wrist using an electro-rheological fluid
JP3075370B2 (ja) 1991-07-26 2000-08-14 シスメックス株式会社 粒子分析用のサンプル扁平流形成装置
US5311290A (en) * 1992-09-30 1994-05-10 Pulp And Paper Research Institute Of Canada Imaging apparatus and method of fiber analysis
US5985216A (en) 1997-07-24 1999-11-16 The United States Of America, As Represented By The Secretary Of Agriculture Flow cytometry nozzle for high efficiency cell sorting
US6149867A (en) 1997-12-31 2000-11-21 Xy, Inc. Sheath fluids and collection systems for sex-specific cytometer sorting of sperm
US6263745B1 (en) * 1999-12-03 2001-07-24 Xy, Inc. Flow cytometer nozzle and flow cytometer sample handling methods
US6825926B2 (en) * 2002-11-19 2004-11-30 International Remote Imaging Systems, Inc. Flow cell for urinalysis diagnostic system and method of making same
DE102005044530B4 (de) 2004-09-16 2010-02-18 Masterrind Gmbh Verfahren zur geschlechtsspezifischen Selektion von Säugerspermatozoen
US20110076712A1 (en) * 2008-06-13 2011-03-31 Xy, Llc. Lubricious microfludic flow path system
EP2267430A1 (de) 2009-06-24 2010-12-29 Masterrind GmbH Vorrichtung und Verfahren zur Selektion von Partikeln

Also Published As

Publication number Publication date
EP2522983A3 (de) 2013-10-09
DK2522983T4 (da) 2022-06-13
HUE027402T2 (en) 2016-09-28
EP2522983B1 (de) 2015-12-16
PL2522983T5 (pl) 2022-09-05
PL2522983T3 (pl) 2016-06-30
DE102011075711A1 (de) 2012-11-15
DK2522983T3 (en) 2016-03-21
EP2522983A2 (de) 2012-11-14
ES2564099T5 (es) 2022-06-17
EP2522983B2 (de) 2022-03-30

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