ES2532030B2 - Method of measuring ex vivo vascular tone and flow chamber - Google Patents
Method of measuring ex vivo vascular tone and flow chamber Download PDFInfo
- Publication number
- ES2532030B2 ES2532030B2 ES201300866A ES201300866A ES2532030B2 ES 2532030 B2 ES2532030 B2 ES 2532030B2 ES 201300866 A ES201300866 A ES 201300866A ES 201300866 A ES201300866 A ES 201300866A ES 2532030 B2 ES2532030 B2 ES 2532030B2
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- measuring
- vascular tone
- flow chamber
- vessel
- tone
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/49—Blood
Abstract
Método de medición del tono vascular ex vivo y cámara de flujo.#La presente invención se refiere a un método de medición del tono vascular ex vivo que comprende: perfundir un flujo constante de una suspensión celular en un circuito abierto que incluye un vaso cuyo tono se desea estudiar, cerrar el circuito, perfundir una solución nutricia de Krebs en el circuito cerrado y aplicar una presión transmural constante, añadir una sustancia vasoactiva y registrar las variaciones de diámetro del vaso mediante un sistema de captación de imágenes situado perpendicularmente al vaso y acoplado a un microscopio.#La invención también se refiere a una cámara de flujo en la que realizar el método de medición del tono vascular que incluye dos capilares de vidrio, un baño, una bomba de infusión, un sistema de presión hidrostática, un manómetro, un sistema de termorregulación de la temperatura del baño y de las soluciones y/o suspensiones utilizadas, un sistema de captación de imágenes y un microscopio.Method of measuring ex vivo vascular tone and flow chamber # The present invention relates to a method of measuring ex vivo vascular tone comprising: perfusing a constant flow of a cell suspension in an open circuit that includes a vessel whose tone it is desired to study, close the circuit, perfuse a nutrient solution of Krebs in the closed circuit and apply a constant transmural pressure, add a vasoactive substance and record the vessel diameter variations by means of an image capture system located perpendicular to the vessel and coupled to a microscope. # The invention also relates to a flow chamber in which to perform the method of measuring vascular tone that includes two glass capillaries, a bath, an infusion pump, a hydrostatic pressure system, a pressure gauge, a thermoregulation system for the bath temperature and the solutions and / or suspensions used, a system for capturing i images and a microscope.
Description
Claims (1)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES201300866A ES2532030B2 (en) | 2013-09-20 | 2013-09-20 | Method of measuring ex vivo vascular tone and flow chamber |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES201300866A ES2532030B2 (en) | 2013-09-20 | 2013-09-20 | Method of measuring ex vivo vascular tone and flow chamber |
Publications (2)
Publication Number | Publication Date |
---|---|
ES2532030A1 ES2532030A1 (en) | 2015-03-23 |
ES2532030B2 true ES2532030B2 (en) | 2015-12-28 |
Family
ID=52683008
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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ES201300866A Active ES2532030B2 (en) | 2013-09-20 | 2013-09-20 | Method of measuring ex vivo vascular tone and flow chamber |
Country Status (1)
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ES (1) | ES2532030B2 (en) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN116045878B (en) * | 2023-03-07 | 2023-06-20 | 中国人民解放军军事科学院军事医学研究院 | Method for detecting vascular diameter change |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4535786A (en) * | 1983-07-25 | 1985-08-20 | Kater John A R | Measurement of body fluid chemistry |
DE3774680D1 (en) * | 1986-12-22 | 1992-01-02 | Siemens Ag | ARRANGEMENT FOR EXAMINING A LIQUID MEDIUM AND METHOD FOR OPERATING THE ARRANGEMENT. |
US5078135A (en) * | 1989-05-31 | 1992-01-07 | Board Of Regents, The University Of Texas System | Apparatus for in vivo analysis of biological compounds in blood or tissue by microdialysis and mass spectrometry |
US6607501B2 (en) * | 2001-05-14 | 2003-08-19 | Reynolds G. Gorsuch | Process and apparatus for utilization of in vivo extracted plasma with tissue engineering devices, bioreactors, artificial organs, and cell therapy applications |
CA2605233C (en) * | 2005-04-29 | 2013-04-23 | Dtherapeutics, Llc | Devices, systems and methods for isometric and isotonic contraction of blood vessels using an isovolumic myograph |
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2013
- 2013-09-20 ES ES201300866A patent/ES2532030B2/en active Active
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ES2532030A1 (en) | 2015-03-23 |
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