ES2532030A1 - Method of measuring ex vivo vascular tone and flow chamber (Machine-translation by Google Translate, not legally binding) - Google Patents

Method of measuring ex vivo vascular tone and flow chamber (Machine-translation by Google Translate, not legally binding) Download PDF

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Publication number
ES2532030A1
ES2532030A1 ES201300866A ES201300866A ES2532030A1 ES 2532030 A1 ES2532030 A1 ES 2532030A1 ES 201300866 A ES201300866 A ES 201300866A ES 201300866 A ES201300866 A ES 201300866A ES 2532030 A1 ES2532030 A1 ES 2532030A1
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ES
Spain
Prior art keywords
measuring
vascular tone
flow chamber
translation
legally binding
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Granted
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ES201300866A
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Spanish (es)
Other versions
ES2532030B2 (en
Inventor
María Teresa TEJERINA SÁNCHEZ
Santiago REDONDO BLASCO
Úrsula Fabiola MEDINA MORENO
Jorge NAVARRO DORADO
Marta RAMAJO MATESANZ
Alexis CANTIZANO GONZÁLEZ
Mario CASTRO PONCE
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Universidad Complutense de Madrid
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Universidad Complutense de Madrid
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Priority to ES201300866A priority Critical patent/ES2532030B2/en
Publication of ES2532030A1 publication Critical patent/ES2532030A1/en
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Publication of ES2532030B2 publication Critical patent/ES2532030B2/en
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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/49—Blood

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Ecology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Method of measuring ex vivo vascular tone and flow chamber. The present invention relates to a method of measuring ex vivo vascular tone comprising: perfusing a constant flow of a cell suspension in an open circuit including a vessel whose tone is to be studied, closing the circuit, perfusing a krebs nutrient solution in the closed circuit and apply a constant transmural pressure, add a vasoactive substance and record the variations in diameter of the vessel by means of an image capture system located perpendicular to the vessel and coupled to a microscope. The invention also relates to a flow chamber in which to perform the method of measuring vascular tone that includes two glass capillaries, a bath, an infusion pump, a hydrostatic pressure system, a manometer, a thermoregulation system of the temperature of the bath and of the solutions and/or suspensions used, a system for capturing images and a microscope. (Machine-translation by Google Translate, not legally binding)

Description

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Claims (1)

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ES201300866A 2013-09-20 2013-09-20 Method of measuring ex vivo vascular tone and flow chamber Active ES2532030B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES201300866A ES2532030B2 (en) 2013-09-20 2013-09-20 Method of measuring ex vivo vascular tone and flow chamber

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES201300866A ES2532030B2 (en) 2013-09-20 2013-09-20 Method of measuring ex vivo vascular tone and flow chamber

Publications (2)

Publication Number Publication Date
ES2532030A1 true ES2532030A1 (en) 2015-03-23
ES2532030B2 ES2532030B2 (en) 2015-12-28

Family

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Family Applications (1)

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ES201300866A Active ES2532030B2 (en) 2013-09-20 2013-09-20 Method of measuring ex vivo vascular tone and flow chamber

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ES (1) ES2532030B2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116045878A (en) * 2023-03-07 2023-05-02 中国人民解放军军事科学院军事医学研究院 Vascular pipe diameter detection equipment

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4535786A (en) * 1983-07-25 1985-08-20 Kater John A R Measurement of body fluid chemistry
US4841974A (en) * 1986-12-22 1989-06-27 Siemens Aktiengesellschaft Apparatus and method for the examination of a liquid medium
WO1990014791A1 (en) * 1989-05-31 1990-12-13 Board Of Regents, The University Of Texas System Apparatus for analysis of biological compounds by mass spectrometry
US20020188240A1 (en) * 2001-05-14 2002-12-12 Gorsuch Reynolds G. Process and apparatus for utilization of in vivo extracted plasma with tissue engineering devices, bioreactors, artificial organs, and cell therapy applications
WO2006119143A2 (en) * 2005-04-29 2006-11-09 Dtherapeutics, Llc Devices, systems and methods for isometric and isotonic contraction of blood vessels using an isovolumic myograph

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4535786A (en) * 1983-07-25 1985-08-20 Kater John A R Measurement of body fluid chemistry
US4841974A (en) * 1986-12-22 1989-06-27 Siemens Aktiengesellschaft Apparatus and method for the examination of a liquid medium
WO1990014791A1 (en) * 1989-05-31 1990-12-13 Board Of Regents, The University Of Texas System Apparatus for analysis of biological compounds by mass spectrometry
US20020188240A1 (en) * 2001-05-14 2002-12-12 Gorsuch Reynolds G. Process and apparatus for utilization of in vivo extracted plasma with tissue engineering devices, bioreactors, artificial organs, and cell therapy applications
WO2006119143A2 (en) * 2005-04-29 2006-11-09 Dtherapeutics, Llc Devices, systems and methods for isometric and isotonic contraction of blood vessels using an isovolumic myograph

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116045878A (en) * 2023-03-07 2023-05-02 中国人民解放军军事科学院军事医学研究院 Vascular pipe diameter detection equipment

Also Published As

Publication number Publication date
ES2532030B2 (en) 2015-12-28

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