ES249137A1 - Reagent and method for determining the coagulability of blood - Google Patents
Reagent and method for determining the coagulability of bloodInfo
- Publication number
- ES249137A1 ES249137A1 ES0249137A ES249137A ES249137A1 ES 249137 A1 ES249137 A1 ES 249137A1 ES 0249137 A ES0249137 A ES 0249137A ES 249137 A ES249137 A ES 249137A ES 249137 A1 ES249137 A1 ES 249137A1
- Authority
- ES
- Spain
- Prior art keywords
- cephalin
- factor
- plasma
- reagent
- bovine
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/86—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood coagulating time or factors, or their receptors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/56—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving blood clotting factors, e.g. involving thrombin, thromboplastin, fibrinogen
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/96—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood or serum control standard
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2496/00—Reference solutions for assays of biological material
- G01N2496/05—Reference solutions for assays of biological material containing blood cells or plasma
Abstract
A reagent for testing the coagulability of blood during treatment with anticoagulent agents comprises thromboplastin in optimal concentration having a Quick's thromboplastin time of at least 35 seconds with normal human plasma in admixture with cephalin or a cephalin substitute, e.g. lecithin. Bovine plasma from which prothrombin, Stuart-factor, proconvertin and antihemophilia-B-factor have been removed and which contains high concentrations of antihemophilia-A and -C-factors, Hagemanfactor, proaccelarin and proconvertin, and calcium chloride may also be present, the latter in an amount sufficient to give the optimal concentration of Ca\sR\sR ions for coagulation after the addition of test plasmas. The reagent may be freeze-dried to give a stable, solid preparation which may be reconstituted before use. The prothrombin may be prepared by known procedures from animal (e.g. bovine, horse, swine) brain and the cephalin from human brain. The bovine plasma may be obtained by the treatment thereof with barium sulphate followed by dialysis to remove oxalate and the carbonic acid/bicarbonate buffer system and adjustment of the pH to from 6-8.ALSO:A reagent for testing the coagulability of blood during treatment with anticoagulent agents comprises thromboplastin in optional concentration having a Quick's thromboplastin time of at least 35 seconds with normal human plasma in admixture with cephalin or a cephalin substitute e.g. lecithin. Bovine plasma from which prothrombin, Stuart-factor, proconvertin and antihaemophilia-b -factor have been removed and which contains high concentrations of antihaemophilia-A and -C-factors, Hageman-factor, proaccelarin and proconvertin, and calcium chloride may also be present, the latter in amounts sufficient to give the optimal concentration of CA++ ions for coagulation after the addition of test plasma. The reagent may be freeze-dried to give a stable, solid preparation which may be reconstituted before use. The prothrombin may be prepared by known methods from animal (e.g. bovine, horse, swine) brain and the cephalin from human brain. The bovine plasma may be obtained by the treatment thereof with barium sulphate followed by dialysis to remove oxalate and the carbonic acid/bicarbonate buffer system and adjustment of the pH to from 6 to 8.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
NO12810458 | 1958-05-16 |
Publications (1)
Publication Number | Publication Date |
---|---|
ES249137A1 true ES249137A1 (en) | 1959-11-16 |
Family
ID=19908436
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES0249137A Expired ES249137A1 (en) | 1958-05-16 | 1959-05-02 | Reagent and method for determining the coagulability of blood |
Country Status (7)
Country | Link |
---|---|
US (1) | US3179567A (en) |
CH (1) | CH408283A (en) |
DE (1) | DE1189763B (en) |
ES (1) | ES249137A1 (en) |
FR (1) | FR1223856A (en) |
GB (1) | GB917012A (en) |
NL (2) | NL123584C (en) |
Families Citing this family (25)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3293134A (en) * | 1961-09-08 | 1966-12-20 | Warner Lambert Pharmaceutical | Diagnostic reagent composition for determining blood coagulation factors and method of use |
US3395210A (en) * | 1964-02-07 | 1968-07-30 | Warner Lambert Pharmaceutical | Lyophilized diagnostic reagent for the determination of blood coagulation factors |
US3486981A (en) * | 1965-03-15 | 1969-12-30 | Roy E Speck | Substances relating to testing of blood-coagulation |
US3228841A (en) * | 1965-06-10 | 1966-01-11 | Warner Lambert Pharmaceutical | Diagnostic reagent composition for determining blood coagulation factors and method of use |
US3880714A (en) * | 1973-07-18 | 1975-04-29 | Warner Lambert Co | Diagnostic reagent |
US3947378A (en) * | 1974-12-23 | 1976-03-30 | Warner-Lambert Company | Adsorbed plasma |
US4002739A (en) * | 1975-02-18 | 1977-01-11 | Warner-Lambert Company | Soluble collagen |
US4038147A (en) * | 1976-06-15 | 1977-07-26 | Becton, Dickinson And Company | Method for detecting endotoxins |
DE2915310A1 (en) * | 1979-04-14 | 1980-10-30 | Behringwerke Ag | NEW PARTIAL THROMBOPLASTINE AND THEIR USE |
DE3150596A1 (en) * | 1981-12-21 | 1983-06-30 | Boehringer Mannheim Gmbh, 6800 Mannheim | METHOD FOR PRODUCING TISSUE HROMBOPLASTIN |
DE3407280A1 (en) * | 1984-02-28 | 1985-09-19 | Gödecke AG, 1000 Berlin | TEST SET FOR DETERMINING THE ACTIVATED PARTIAL THROMBOPLASTIN TIME (PTT) WITH INCREASED HEPARINE SENSITIVITY |
DE3413311A1 (en) * | 1984-04-09 | 1985-10-17 | Behringwerke Ag, 3550 Marburg | REAGENT FOR DETERMINING THROMBOPLASTIN TIME |
US4946775A (en) * | 1985-09-05 | 1990-08-07 | Yin E Thye | Composition, kit and method for assaying heparin and a method for making the composition |
US4948724A (en) * | 1985-09-05 | 1990-08-14 | Yin E Thye | Composition, kit and method for assaying heparin and a method for making the composition |
US4851336A (en) * | 1985-09-05 | 1989-07-25 | Yin E Thye | Composition, kit, and method for assaying heparinano a method for making the composition |
US4755461A (en) * | 1986-04-17 | 1988-07-05 | Bio/Data Corporation | Tableted blood plasma microconcentrated thromboplastin coagulation reagent |
US5314695A (en) * | 1990-11-13 | 1994-05-24 | Corvas International, Inc. | Tissue factor based prothrombin time reagent |
US6203816B1 (en) * | 1990-11-13 | 2001-03-20 | Corvas International, Inc. | Tissue factor based prothrombin time reagent |
AU744287B2 (en) * | 1990-11-13 | 2002-02-21 | Corvas International, Inc. | Tissue factor based prothrombin time reagent |
US6248353B1 (en) | 1999-12-10 | 2001-06-19 | Dade Behring Inc. | Reconstitution of purified membrane proteins into preformed liposomes |
US6596543B2 (en) | 2001-03-22 | 2003-07-22 | Dade Behring Inc. | Use of liposomes of defined composition and size for the preparation of prothrombin time reagents |
IS2809B (en) * | 2011-03-08 | 2012-10-15 | Haskoli Islands | Method for monitoring anticoagulant therapy |
CA3078625C (en) | 2017-10-09 | 2023-01-17 | Terumo Bct Biotechnologies, Llc | Lyophilization container and method of using same |
EP3937878A2 (en) | 2019-03-14 | 2022-01-19 | Terumo BCT Biotechnologies, LLC | Lyophilization loading tray assembly and system |
CN112481355B (en) * | 2020-11-16 | 2023-05-30 | 武汉市长立生物技术有限责任公司 | Liquid prothrombin time determination kit and preparation method thereof |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US2687980A (en) * | 1948-09-15 | 1954-08-31 | Ernest W Blanchard | Thromboplastin product and method for preparing same |
US2847350A (en) * | 1954-05-27 | 1958-08-12 | Ortho Pharma Corp | Preparation of highly active thromboplastin |
-
0
- NL NL238873D patent/NL238873A/xx unknown
- NL NL123584D patent/NL123584C/xx active
-
1959
- 1959-03-31 GB GB10940/59A patent/GB917012A/en not_active Expired
- 1959-04-30 DE DEO6747A patent/DE1189763B/en active Pending
- 1959-05-02 ES ES0249137A patent/ES249137A1/en not_active Expired
- 1959-05-04 CH CH7286559A patent/CH408283A/en unknown
- 1959-05-05 FR FR794205A patent/FR1223856A/en not_active Expired
- 1959-05-12 US US812682A patent/US3179567A/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
DE1189763B (en) | 1965-03-25 |
FR1223856A (en) | 1960-06-21 |
NL123584C (en) | |
US3179567A (en) | 1965-04-20 |
NL238873A (en) | |
CH408283A (en) | 1966-02-28 |
GB917012A (en) | 1963-01-30 |
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