ES2370219A1 - Use of an anti-enzyme composition for pr¿teas. (Machine-translation by Google Translate, not legally binding) - Google Patents

Use of an anti-enzyme composition for pr¿teas. (Machine-translation by Google Translate, not legally binding) Download PDF

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Publication number
ES2370219A1
ES2370219A1 ES201030738A ES201030738A ES2370219A1 ES 2370219 A1 ES2370219 A1 ES 2370219A1 ES 201030738 A ES201030738 A ES 201030738A ES 201030738 A ES201030738 A ES 201030738A ES 2370219 A1 ES2370219 A1 ES 2370219A1
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blackening
days
prostheses
translation
machine
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ES2370219B1 (en
Inventor
Enrique Olmos Aranda
Carlos Enrique Álvarez González
Marino Jesus Fernández Falcon
Mercedes Hernandez Gonzalez
Nieves Fernandez Garcia
Ana María Jiménez Hurtado
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Consejo Superior de Investigaciones Cientificas CSIC
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N3/00Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
    • A01N3/02Keeping cut flowers fresh chemically
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23BPRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
    • A23B7/00Preservation or chemical ripening of fruit or vegetables
    • A23B7/10Preserving with acids; Acid fermentation

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Toxicology (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Cultivation Of Plants (AREA)

Abstract

Use of an anti-blackening composition for prosthetics. The present invention relates to the use of an anti-blackening composition for protids, comprising ascorbic acid. (Machine-translation by Google Translate, not legally binding)

Description

Uso de una composición anti-ennegrecimiento para próteas.Use of a composition anti-blackening for prostheses.

La presente invención se refiere al uso de una composición anti-ennegrecimiento para próteas, que comprende al ácido ascórbico.The present invention relates to the use of a anti-blackening composition for prostheses, which It comprises ascorbic acid.

Estado de la técnica anteriorPrior art

Las próteas son plantas arbustivas pertenecientes a la familia de las proteáceas procedentes principalmente de África del Sur y Australia. Entre las características más importantes que nos ofrece esta flor están: la floración en la época de otoño - invierno, que es cuando escasean otras flores, y la larga duración de la flor una vez cortada.The prostheses are shrubby plants belonging to the family of proteinaceous originating mainly from South Africa and Australia. Between the most important features that this flower offers us are: flowering in the autumn - winter season, which is when they are scarce other flowers, and the long duration of the flower once cut.

Este cultivo fue introducido en las islas Canarias aproximadamente en el año 1998 como una alternativa a otros cultivos. Las próteas son exportadas principalmente desde la isla de La Palma a Europa, mercado que siempre demanda alta calidad, aunque en la actualidad se está empezando a vender en el mercado peninsular. Todas las flores son enviadas por avión, lo que garantiza que lleguen al mercado en unas condiciones de calidad y frescura inmejorables. En la actualidad, en lo referente a nuestro país, alrededor de 1.600.000 tallos han sido exportados.This crop was introduced in the islands Canary Islands approximately in the year 1998 as an alternative to others crops. The proteins are exported mainly from the island of La Palma to Europe, a market that always demands high quality, although currently it is starting to sell in the market peninsular. All flowers are sent by plane, which guarantees that they reach the market in quality conditions and unbeatable freshness. At present, in relation to our country, about 1,600,000 stems have been exported.

Estas plantas presentan un importante problema en dos variedades que se encuentran entre las más solicitadas para su exportación. Este problema consiste en que dichos cultivares presentan ennegrecimientos post-cosecha que disminuyen su calidad comercial. Estos ennegrecimientos podrían estar relacionados con la actividad de las enzimas polifenol oxidasa y peroxidasa, por lo que se han llevado a cabo estudios de los sistemas antioxidantes de las plantas afectadas a fin de intentar resolver este proble-
ma.
These plants present an important problem in two varieties that are among the most requested for export. This problem consists in the fact that these cultivars have post-harvest blackouts that decrease their commercial quality. These blackening could be related to the activity of the polyphenol oxidase and peroxidase enzymes, so studies of the antioxidant systems of the affected plants have been carried out in order to try to solve this problem.
ma.

La solución a este problema es algo muy demandado por los agricultores a fin de hacer aptos estos cultivares para su exportación, debido a que dicho desorden causa grandes pérdidas a este sector. Resaltar que este mismo problema lo presentan también las próteas cultivadas en Australia y Sudáfrica.The solution to this problem is something very demanded by farmers in order to make these cultivars fit for export, because such disorder causes large losses to this sector. Highlight that this same problem they also present the prostheses grown in Australia and South Africa.

En la bibliografía existente en relación a este desorden, ha sido correlacionado con el agotamiento de niveles de hidrato de carbono de hojas en el cultivar de prótea "Sylvia" (Stephens, I.A., Jacobs, G., Holcroft, D.M. (2001). Postharvest Biology and Technology 23: 237-240; Stephens, I.A., Holcroft, D.M., Jacobs, G (2000). Acta Hort. 545: 205-214), pero las soluciones ofrecidas no han sido satisfactorias para las cooperativas. Otro tratamiento que se ha intentado ha sido con etanol (Crick, S.G., McConchie, R. (1999). Postharvest Biology and Technology 17: 227-231) pero este tratamiento propuesto no es aplicable en campo por los agricultores.In the existing literature in relation to this disorder, it has been correlated with the depletion of leaf carbohydrate levels in the "Sylvia" prostate cultivar ( Stephens, IA, Jacobs, G., Holcroft, DM (2001). Postharvest Biology and Technology 23: 237-240; Stephens, IA, Holcroft, DM, Jacobs, G (2000). Acta Hort. 545: 205-214 ), but the solutions offered have not been satisfactory for cooperatives. Another treatment that has been tried has been with ethanol ( Crick, SG, McConchie, R. (1999). Postharvest Biology and Technology 17: 227-231 ) but this proposed treatment is not applicable in the field by farmers.

Por lo tanto es necesario desarrollar una nueva vía que permita retrasar el máximo tiempo posible el ennegrecimiento de este tipo de plantas.Therefore it is necessary to develop a new a way to delay blackening as long as possible of this type of plants.

Explicación de la invenciónExplanation of the invention.

La presente invención se refiere al uso de una composición anti-ennegrecimiento para próteas, que comprende al ácido ascórbico.The present invention relates to the use of a anti-blackening composition for prostheses, which It comprises ascorbic acid.

Una realización preferida se refiere al uso de una composición anti-ennegrecimiento para próteas que comprende ácido ascórbico disuelto en agua.A preferred embodiment refers to the use of an anti-blackening composition for prostheses comprising ascorbic acid dissolved in water.

Según otra realización preferida el ácido ascórbico se añade en una concentración de 3 a 10 g/l, preferiblemente de 3 a 7 g/l, más preferiblemente en una concentración de 5 g/l.According to another preferred embodiment the acid ascorbic is added in a concentration of 3 to 10 g / l, preferably from 3 to 7 g / l, more preferably in a concentration of 5 g / l.

Otra realización preferida se refiere al uso de la composición anti-ennegrecimiento para las variedades Pink Ice y Susara de próteas.Another preferred embodiment relates to the use of the anti-blackening composition for Pink Ice and Susara varieties of prostheses.

A lo largo de la descripción y las reivindicaciones la palabra "comprende" y sus variantes no pretenden excluir otras características técnicas, aditivos, componentes o pasos. Para los expertos en la materia, otros objetos, ventajas y características de la invención se desprenderán en parte de la descripción y en parte de la práctica de la invención. Las siguientes figuras y ejemplos se proporcionan a modo de ilustración, y no se pretende que sean limitativos de la presente invención.Throughout the description and the claims the word "comprises" and its variants not they intend to exclude other technical characteristics, additives, components or steps. For those skilled in the art, other objects, advantages and features of the invention will be partly detached of the description and in part of the practice of the invention. The following figures and examples are provided by way of illustration, and are not intended to be limiting of the present invention.

Descripción de las figurasDescription of the figures

Fig. 1. Detalle de ennegrecimiento foliar en hoja joven de prótea.Fig. 1. Detail of leaf blackening in young leaf of prostate.

Fig. 2. Cv. Pink Ice 11 días control. Flor de cv. Pink Ice tras 11 días cortada y sin tratamiento.Fig. 2. Cv. Pink Ice 11 days control. Flower of cv. Pink Ice after 11 days cut and without treatment.

Fig. 3. Cv. Pink Ice 11 días rociada (izquierda) y sumergida (derecha). Flor de cv. Pink Ice tras 11 días cortada, rociada diariamente (izquierda), y con el tallo sumergido (derecha). Ambas presentan un aspecto válido aunque las sumergidas presentan un mejor aspecto que las rociadas.Fig. 3. Cv. Pink Ice 11 days sprayed (left) and submerged (right). Cv flower. Pink Ice after 11 days cut, sprayed daily (left), and with the stem submerged (right). Both have a valid appearance although the submerged have a Better looking than the sprays.

Fig. 4. Cv. Pink Ice sumergida 13 días. Flor de cv. Pink Ice tras 13 días cortada, sumergida. Su aspecto presenta ennegrecimiento foliar aunque el capullo floral aún presenta un aspecto válido.Fig. 4. Cv. Pink Ice submerged 13 days. Flower of cv. Pink Ice after 13 days cut, submerged. Its appearance presents leaf blackening although the flower bud still presents a valid aspect.

Fig. 5. Cv. Susara control (izquierda) y rociada (derecha) 13 días. Flor de cv. Susara sin tratar (izquierda) y rociada diariamente (derecha) 13 días. La flor rociada aún presenta tras 13 días del corte un aspecto válido tanto en las hojas como en su capullo floral.Fig. 5. Cv. Susara control (left) and sprayed (right) 13 days. Cv flower. Susara untreated (left) and sprayed daily (right) 13 days. The sprayed flower still presents after 13 days of the cut a valid aspect both in the sheets and in Your floral bud.

Fig. 6. Cv. Susara sumergida 13 días. En esta figura se presenta el aspecto de este cv tras 13 días sumergido que es peor que el de esta flor cuando el tratamiento es por rociado.Fig. 6. Cv. Susara submerged 13 days. In this figure shows the appearance of this cv after 13 days submerged that it is worse than that of this flower when the treatment is for sprayed

Fig. 7. Cv. Susara rociada (izquierda) y sumergida (derecha) 11 días.Fig. 7. Cv. Susara sprayed (left) and submerged (right) 11 days.

Fig. 8. Cv. Susara control (izquierda) y rociada (derecha) 13 días.Fig. 8. Cv. Susara control (left) and sprayed (right) 13 days.

Ejemplos Examples

A continuación se ilustrará la invención mediante unos ensayos realizados por los inventores que describen la actividad antifúngica de la composición desarrolla en la presente invención, así como el procedimiento de conservación de próteas mediante dicha composición.The invention will be illustrated below by means of tests carried out by the inventors that describe the antifungal activity of the composition developed herein invention, as well as the procedure of preservation of prostheses by said composition.

Ejemplo 1Example one

La experiencia realizada ha consistido en tratar con la composición anti-ennegrecimiento los dos cultivares más afectados en la Palma por este problema, que son Pink Ice y Susara.The experience has been to try with the anti-blackening composition both cultivars most affected in La Palma by this problem, which are Pink Ice and Susara.

Estas variedades fueron tratadas el mismo día que fueron cortadas. El tratamiento se dio de dos for-
mas:
These varieties were treated the same day they were cut. The treatment was given in two ways.
more:

--
Sumergiendo los tallos cortados de las flores en dicho compuesto desde el mismo día del corte de la flor.Immersing the cut stems of the flowers in said compound from the same day of the cut of the flower.

--
Rociando las plantas con dicho compuesto cada 24 h.Spraying the plants with said compound every 24 h.

Los resultados obtenidos fueron diferentes dependiendo del cultivar:The results obtained were different Depending on the cultivar:

1. Pink Ice1. Pink Ice

En este cultivar es donde aparece primero el ennegrecimiento, y su efecto es mucho más drástico que en cv. Susara.In this cultivar is where the blackening, and its effect is much more drastic than in cv. Susara

1.1. Tras 8 días de corte1.1. After 8 days of cutting

Las plantas control (no tratadas) presentan síntomas de ennegrecimiento y pierden por tanto su valor comercial.Control plants (untreated) have blackening symptoms and therefore lose their value commercial.

Las plantas tratadas sumergiendo los tallos en una solución con ácido ascórbico de concentración 5 g/l disuelto en agua (Figura 2 derecha) presentan las siguientes características:Plants treated by immersing the stems in a solution with ascorbic acid of 5 g / l concentration dissolved in water (Figure 2 right) have the following characteristics:

\bullet?
No se observan síntomas de ennegrecimiento.No symptoms of blackening

\bullet?
El capullo floral está más vivo, sin síntomas de desecación que en las plantas rociadas.The floral bud is more alive, no drying symptoms than in sprayed plants.

Cuando se les dio el tratamiento rociando las flores con ácido ascórbico de concentración 5 g/l disuelto en agua (Figura 2 izquierda), el resultado fue el siguiente:When the treatment was given by spraying the flowers with 5 g / l concentration ascorbic acid dissolved in water (Figure 2 left), the result was as follows:

\bullet?
No se observan síntomas de ennegrecimiento.No symptoms of blackening

\bullet?
El capullo floral está más seco que cuando eran sumergidas.The flower bud is drier That when they were submerged.
1.2. Tras 11 días de corte1.2. After 11 days of cut

Las flores controles están completamente ennegrecidas y por ello se retiran (Figura 3) y las que están sumergidas comienzan a presentar síntomas de ennegrecimiento (Figura 4 derecha).The control flowers are completely blackened and therefore removed (Figure 3) and those that are submerged begin to show symptoms of blackening (Figure 4 right).

Las flores que han sido rociadas, comienzan a presentar síntomas de ennegrecimiento pero presentan peor aspecto que las sumergidas (Figura 4 izquier-
da).
Flowers that have been sprayed begin to show blackening symptoms but look worse than submerged ones (Figure 4 left).
gives).

El capullo floral sigue siendo más vivo en las sumergidas que las rociadas.The flower bud is still more alive in the submerged than sprinkled.

1.3. Tras 13 días de corte1.3. After 13 days of cut

Las que han sido rociadas son ya flores de desecho, y en las que fueron sumergidas, el capullo floral aún se mantiene vistoso (Figura 5).Those that have been sprayed are already flowers of waste, and in which they were submerged, the flower bud is still keeps it colorful (Figure 5).

2. Susara2. Susara 2.1. Tras 8 días de corte2.1. After 8 days of cutting

Ni las plantas controles (Figura 6) ni las tratadas presentan ennegrecimiento.Neither the control plants (Figure 6) nor the treated show blackening.

2.2. Tras 11 días de corte2.2. After 11 days of cut

Las flores que están sumergidas están totalmente ennegrecidas, hay que desecharlas (Figura 7 dere-
cha).
The flowers that are submerged are completely blackened, they must be discarded (Figure 7 right)
cha).

Las flores que están rociadas no presentan ningún ennegrecimiento (Figura 7 izquierda).The flowers that are sprayed do not show any blackening (Figure 7 left).

2.3. Tras 13 días de corte2.3. After 13 days of cut

Las flores rociadas (Figura 8 derecha) presentan aún mejor aspecto que las controles (Figura 8 izquierda) que ya son desechables. Sprayed flowers (Figure 8 right) look even better than the controls (Figure 8 left) that are already disposable.

Claims (6)

1. Uso de una composición anti-ennegrecimiento de próteas, que comprende ácido ascórbico.1. Use of a composition anti-blackening of prostheses, which includes acid ascorbic 2. Uso la composición anti-ennegrecimiento de próteas, según la reivindicación 1, caracterizado porque comprende ácido ascórbico disuelto en agua.2. Use the anti-blackening composition of prostheses according to claim 1, characterized in that it comprises ascorbic acid dissolved in water. 3. Uso de la composición anti-ennegrecimiento de próteas: según la reivindicación 2, donde el ácido ascórbico tiene una concentración de 3 a 10 g/l.3. Use of the composition anti-blackening of prostheses: according to claim 2, wherein the ascorbic acid has a concentration from 3 to 10 g / l. 4. Uso de la composición anti-ennegrecimiento de próteas: según cualquiera de las reivindicaciones 2 ó 3, donde el ácido ascórbico tiene una concentración de 3 a 7 g/l.4. Use of the composition anti-blackening of prostheses: according to any of claims 2 or 3, wherein the ascorbic acid has a concentration of 3 to 7 g / l. 5. Uso de la composición anti-ennegrecimiento de próteas: según cualquiera de las reivindicaciones 2 a 4, donde el ácido ascórbico tiene una concentración de 5 g/l.5. Use of the composition anti-blackening of prostheses: according to any of claims 2 to 4, wherein the ascorbic acid has a concentration of 5 g / l. 6. Uso de la composición anti-ennegrecimiento de próteas según cualquiera de las reivindicaciones 1 a 4, para las variedades Pink Ice y Susara.6. Use of the composition anti-blackening of prostates according to any of claims 1 to 4, for the Pink Ice and Susara
ES201030738A 2010-05-18 2010-05-18 USE OF AN ANTI-BLACK COMPOSITION FOR PROSTS. Active ES2370219B1 (en)

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1383272A (en) * 1972-04-12 1974-02-12 Canadian Patents Dev Preservation of cut blooms
JPH0393701A (en) * 1989-09-06 1991-04-18 Shinagawa Nenryo Kk Freshness preserving agent for cut flower
CN1129058A (en) * 1995-09-30 1996-08-21 赵国晶 Non-toxic fresh flower antistaling agent and its preparation method
CN1486605A (en) * 2003-08-05 2004-04-07 湖南农业大学 Fancy candles & fresh flower preservative

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1383272A (en) * 1972-04-12 1974-02-12 Canadian Patents Dev Preservation of cut blooms
JPH0393701A (en) * 1989-09-06 1991-04-18 Shinagawa Nenryo Kk Freshness preserving agent for cut flower
CN1129058A (en) * 1995-09-30 1996-08-21 赵国晶 Non-toxic fresh flower antistaling agent and its preparation method
CN1486605A (en) * 2003-08-05 2004-04-07 湖南农业大学 Fancy candles & fresh flower preservative

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Crick S. G et al. "Ethanol vapour reduces leaf blackening in cut flower Protea `Pink Ice" stems". Postharvest Biology and Technology. Nov 1999. Vol. 17, Nº. 3, páginas 227-231. *
Stephens A. et al. "Glucose prevents leaf blackening in `Sylvia" proteas". Postharvest Biology and Technology. Dic 2001. Vol. 23, Nº. 3, páginas 237-240. *

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