ES2359069T3 - Método para purificar adn plásmido. - Google Patents
Método para purificar adn plásmido. Download PDFInfo
- Publication number
- ES2359069T3 ES2359069T3 ES05739527T ES05739527T ES2359069T3 ES 2359069 T3 ES2359069 T3 ES 2359069T3 ES 05739527 T ES05739527 T ES 05739527T ES 05739527 T ES05739527 T ES 05739527T ES 2359069 T3 ES2359069 T3 ES 2359069T3
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- Prior art keywords
- dna
- plasmid
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- plasmid dna
- composition
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M27/00—Drainage appliance for wounds or the like, i.e. wound drains, implanted drains
- A61M27/002—Implant devices for drainage of body fluids from one part of the body to another
- A61M27/008—Implant devices for drainage of body fluids from one part of the body to another pre-shaped, for use in the urethral or ureteral tract
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M25/00—Catheters; Hollow probes
- A61M25/10—Balloon catheters
- A61M2025/1043—Balloon catheters with special features or adapted for special applications
- A61M2025/105—Balloon catheters with special features or adapted for special applications having a balloon suitable for drug delivery, e.g. by using holes for delivery, drug coating or membranes
Landscapes
- Health & Medical Sciences (AREA)
- Hematology (AREA)
- Animal Behavior & Ethology (AREA)
- Otolaryngology (AREA)
- Engineering & Computer Science (AREA)
- Anesthesiology (AREA)
- Biomedical Technology (AREA)
- Ophthalmology & Optometry (AREA)
- Heart & Thoracic Surgery (AREA)
- Life Sciences & Earth Sciences (AREA)
- Urology & Nephrology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Materials For Medical Uses (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Prostheses (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US55535404P | 2004-03-23 | 2004-03-23 | |
| US563008P | 2004-04-19 | ||
| WOEP2004/011437 | 2004-09-17 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2359069T3 true ES2359069T3 (es) | 2011-05-18 |
Family
ID=34963423
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES05739527T Expired - Lifetime ES2359069T3 (es) | 2004-03-23 | 2005-04-19 | Método para purificar adn plásmido. |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US7445642B2 (https=) |
| EP (1) | EP1727583A1 (https=) |
| JP (1) | JP2007530148A (https=) |
| AU (1) | AU2005228951A1 (https=) |
| CA (1) | CA2558922A1 (https=) |
| ES (1) | ES2359069T3 (https=) |
| WO (1) | WO2005094930A1 (https=) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2025252480A1 (en) * | 2024-06-07 | 2025-12-11 | F. Hoffmann-La Roche Ag | Method for purifying plasmid dna |
Families Citing this family (41)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1056501B1 (en) | 1998-02-24 | 2005-11-16 | Boston Scientific Limited | High flow rate dialysis catheters and related methods |
| US7056338B2 (en) | 2003-03-28 | 2006-06-06 | Conor Medsystems, Inc. | Therapeutic agent delivery device with controlled therapeutic agent release rates |
| AU2004226327A1 (en) | 2003-03-28 | 2004-10-14 | Innovational Holdings, Llc | Implantable medical device with beneficial agent concentration gradient |
| JP4646808B2 (ja) * | 2003-12-02 | 2011-03-09 | 株式会社日立メディコ | 超音波診断装置 |
| US8764729B2 (en) * | 2004-04-21 | 2014-07-01 | Acclarent, Inc. | Frontal sinus spacer |
| US7550012B2 (en) * | 2005-08-31 | 2009-06-23 | Cook Ireland Limited | Stent for implantation |
| US7789915B2 (en) * | 2005-08-31 | 2010-09-07 | Vance Products Incorporated | Stent for implantation |
| US20080051759A1 (en) * | 2006-08-24 | 2008-02-28 | Boston Scientific Scimed, Inc. | Polycarbonate polyurethane venous access devices |
| US20080119823A1 (en) * | 2006-11-21 | 2008-05-22 | Crank Justin M | Injection Tube for Jet Injection Device |
| NL1032961C2 (nl) * | 2006-11-29 | 2008-06-04 | Overtoom Ltd | Systeem van apparaten voor het behandelen van de ureter en/of de pyelo-ureterovergang en een terugslagklep voor een dergelijk apparaat. |
| JP2010522023A (ja) * | 2007-03-20 | 2010-07-01 | ボストン サイエンティフィック サイムド，インコーポレイテッド | 前立腺に対して有益な治療薬を放出するための泌尿器科学的医療機器 |
| US20090187254A1 (en) * | 2007-12-19 | 2009-07-23 | Boston Scientific Scimed, Inc. | Urological medical devices for release of urologically beneficial agents |
| US8048171B2 (en) * | 2008-03-27 | 2011-11-01 | Boston Scientific Scimed, Inc. | Ureteral stents for release of urologically beneficial agents |
| US10039907B2 (en) * | 2008-04-14 | 2018-08-07 | Innoventions Ltd. | Device, system, and method for releasing substances in a body cavity |
| CA3120995A1 (en) * | 2008-10-10 | 2010-04-15 | Implantica Patent Ltd. | Implantable device for internal urinary control |
| US20100256546A1 (en) * | 2009-04-03 | 2010-10-07 | Davis Scott A | Polycarbonate Polyurethane Venous Access Devices Having Enhanced Strength |
| WO2011038235A1 (en) * | 2009-09-24 | 2011-03-31 | Duke University | Methods and apparatuses for generating a steerable pressure field in a shock wave lithotripter |
| US9211182B2 (en) | 2009-11-20 | 2015-12-15 | E2, Llc | Anti-reflux devices and methods for treating gastro-esophageal reflux disease (GERD) |
| US9050435B2 (en) | 2011-03-22 | 2015-06-09 | Angiodynamics, Inc. | High flow catheters |
| US9999746B2 (en) | 2011-03-22 | 2018-06-19 | Angiodynamics, Inc. | High flow catheters |
| US10219889B2 (en) * | 2011-06-10 | 2019-03-05 | Boston Scientific Scimed, Inc. | Balloon expandable ureteral stent |
| US9259517B2 (en) * | 2012-04-10 | 2016-02-16 | Boston Scientific Scimed, Inc. | Ureteral stent with drug delivery reservoir |
| JP6463265B2 (ja) | 2012-07-20 | 2019-01-30 | クック・メディカル・テクノロジーズ・リミテッド・ライアビリティ・カンパニーＣｏｏｋ Ｍｅｄｉｃａｌ Ｔｅｃｈｎｏｌｏｇｉｅｓ Ｌｌｃ | スリーブを有する植え込み型医療デバイス |
| TWI503097B (zh) * | 2012-10-23 | 2015-10-11 | Spirit Spine Holdings Corp Inc | Bone fixation device |
| US9433765B2 (en) * | 2012-11-05 | 2016-09-06 | Indiana University Research & Technology Corporation | System and method for pyeloplasty internal-external stent |
| US9498356B2 (en) | 2012-12-19 | 2016-11-22 | Cook Medical Technologies, LLC | Flexible stent and delivery system |
| WO2014160744A1 (en) * | 2013-03-26 | 2014-10-02 | Innovia Llc | Pacemaker lead and other medical implant devices |
| US20150273120A1 (en) * | 2014-03-26 | 2015-10-01 | Boston Scientific Scimed, Inc. | Medical devices for drainage |
| WO2015189156A1 (en) * | 2014-06-12 | 2015-12-17 | Antonio Sambusseti | Device with a j-shaped end and with an inflatable balloon for use as support for devices for tissue reconstruction of excised tissues of the renal pelvis and/or of the ureteropelvic junction |
| US20160135941A1 (en) * | 2014-06-18 | 2016-05-19 | Xlumena, Inc. | Biliary stents and methods |
| US10667931B2 (en) | 2014-07-20 | 2020-06-02 | Restore Medical Ltd. | Pulmonary artery implant apparatus and methods of use thereof |
| US9763814B2 (en) | 2014-10-24 | 2017-09-19 | Cook Medical Technologies Llc | Elongate medical device |
| EP3277363B1 (en) * | 2015-03-30 | 2019-06-19 | TARIS Biomedical LLC | Devices for local delivery of drug to upper urinary tract |
| US11771434B2 (en) | 2016-09-28 | 2023-10-03 | Restore Medical Ltd. | Artery medical apparatus and methods of use thereof |
| IL271184B2 (en) | 2017-06-05 | 2024-04-01 | Restore Medical Ltd | Fixed-length, double-walled stent-like device and methods of using the same |
| WO2020018653A1 (en) * | 2018-07-18 | 2020-01-23 | Marblehead Medical Llc | Internal carotid artery thrombectomy devices and methods |
| GB2577052B (en) | 2018-09-11 | 2021-04-28 | Strait Access Tech Holdings Pty Ltd | Expandable sleeved stent and method of making such stent |
| EP3897803B1 (en) | 2018-12-19 | 2026-01-28 | Covidien LP | Internal carotid artery thrombectomy devices |
| WO2020165906A1 (en) * | 2019-02-14 | 2020-08-20 | Technion Research & Development Foundation Limited | Composition, drug delivery device and method for local delivery of an active agent |
| KR102337461B1 (ko) * | 2021-01-18 | 2021-12-13 | 충남대학교병원 | 팽창식 고정구조를 가지는 배액관 |
| CN116509608A (zh) * | 2023-06-20 | 2023-08-01 | 天津市泌尿外科研究所 | 一种三棱型排石防逆流方便取出型输尿管支架 |
Family Cites Families (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1186192A (en) * | 1982-01-27 | 1985-04-30 | David S. Hickey | Urethral catheter |
| JPS6211464A (ja) * | 1985-02-18 | 1987-01-20 | 株式会社塚田メディカル・リサーチ | 膀胱用薬液持続注入カテ−テル |
| DE3741832A1 (de) * | 1987-12-10 | 1989-06-22 | Hoene Eberhard | Mit dynamischem haubenventil bestueckte innere harnleiterschiene zur verhinderung von urin-rueckfluss in die oberen harnwege |
| US4932938A (en) * | 1989-05-05 | 1990-06-12 | Medical Engineering Corporation | Urethral indwelling catheter with incontinence control |
| US5380270A (en) * | 1990-12-07 | 1995-01-10 | Willy Rusch Ag | Ureteral catheter |
| US5141502A (en) * | 1991-08-28 | 1992-08-25 | Macaluso Jr Joseph N | Ureteral stent |
| US5441515A (en) * | 1993-04-23 | 1995-08-15 | Advanced Cardiovascular Systems, Inc. | Ratcheting stent |
| DE4427443A1 (de) * | 1994-08-03 | 1996-02-08 | Sachse Hans E | Katheter mit Wandfensterung |
| US5624395A (en) * | 1995-02-23 | 1997-04-29 | Cv Dynamics, Inc. | Urinary catheter having palpitatable valve and balloon and method for making same |
| US6364868B1 (en) * | 1995-08-02 | 2002-04-02 | The Trustees Of Columbia University In The City Of New York | Ureteral catheter and tissue expander and method of megaureter creation |
| US6042535A (en) * | 1997-07-17 | 2000-03-28 | Srs Medical Systems, Inc. | Flow-around valve |
| US5916195A (en) * | 1998-02-04 | 1999-06-29 | Argomed Ltd. | Internal catheter |
| US6530894B1 (en) * | 1999-11-16 | 2003-03-11 | Coaxia, Inc. | Aortic shunt with spinal perfusion and cooling device |
| US6545097B2 (en) * | 2000-12-12 | 2003-04-08 | Scimed Life Systems, Inc. | Drug delivery compositions and medical devices containing block copolymer |
| US6706013B1 (en) * | 2001-06-29 | 2004-03-16 | Advanced Cardiovascular Systems, Inc. | Variable length drug delivery catheter |
| US6949125B2 (en) * | 2002-04-16 | 2005-09-27 | Boston Scientific Scimed, Inc. | Ureteral stent with end-effector and related methods |
| DE10305553B4 (de) * | 2003-02-10 | 2005-11-03 | Lothar Dr.med. Göbel | Vorrichtung zur Tamponade von Körperhöhlen |
| US7182745B2 (en) * | 2003-03-25 | 2007-02-27 | Boston Scientific Scimed, Inc. | Retaining stent |
-
2005
- 2005-03-22 EP EP05729527A patent/EP1727583A1/en not_active Withdrawn
- 2005-03-22 JP JP2007505061A patent/JP2007530148A/ja active Pending
- 2005-03-22 US US11/085,087 patent/US7445642B2/en not_active Expired - Fee Related
- 2005-03-22 CA CA002558922A patent/CA2558922A1/en not_active Abandoned
- 2005-03-22 AU AU2005228951A patent/AU2005228951A1/en not_active Abandoned
- 2005-03-22 WO PCT/US2005/009335 patent/WO2005094930A1/en not_active Ceased
- 2005-04-19 ES ES05739527T patent/ES2359069T3/es not_active Expired - Lifetime
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2025252480A1 (en) * | 2024-06-07 | 2025-12-11 | F. Hoffmann-La Roche Ag | Method for purifying plasmid dna |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2007530148A (ja) | 2007-11-01 |
| CA2558922A1 (en) | 2005-10-13 |
| WO2005094930A1 (en) | 2005-10-13 |
| AU2005228951A1 (en) | 2005-10-13 |
| US7445642B2 (en) | 2008-11-04 |
| US20050234388A1 (en) | 2005-10-20 |
| EP1727583A1 (en) | 2006-12-06 |
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