ES2328758B1 - BACTERIOLOGICAL COMPOUND ACCELERATING ENZYMATIC OF THE DECOMPOSITION OF ORGANIC MATTER. - Google Patents

Abstract

Accelerating Enzymatic Bacteriological Compound of the decomposition of organic matter.

The recommended invention is a product of biological nature designed for a wide range of products biological whose active ingredient is developed by means of a selection of non-pathogenic microorganisms and enzymes with the corresponding absorbent, whose function is to complete the degradation of organic matter until the last stages, both in the aerobic phase as in the anaerobic phase. The compound object of the invention accelerates extremely the decomposition of matter organic, through the action of enzymes secreted by bacteria special, thus reducing the COD and BOD 5 of leachate.

Description

Accelerating Enzymatic Bacteriological Compound of the decomposition of organic matter.

This Invention Patent consists of as indicated in its statement, in a "Bacteriological Compound Enzymatic accelerator of matter breakdown organic ", whose new formulation characteristics composition and design comply with the regulations with a security and maximum efficiency

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More specifically, the recommended invention is a product of a biological nature, designed for a wide range of biological products, whose active ingredient is developed through a selection of non-pathogenic microorganisms and enzymes, with the corresponding absorbent, whose function is to complete the degradation of organic matter until the last stages, both in the aerobic phase and in the anaerobic phase. The compound object of the invention, accelerates the decomposition extremely of organic matter by the action of enzymes secreted by special bacteria, reducing COD and BOD 5 of leachate

The organic remains are composed of proteins, carbohydrates, fatty substances and fibers, which solidify and make its fluidization and reduction very difficult. Part of these remains is made up of insoluble solids that bacteria cannot attack, unless it is very slowly. Thanks to the product object of the invention accelerates extremely the decomposition of organic matter through the action of enzymes secreted by special bacteria.

In the State of the Art there are products similar, as described and claimed in the Patent Spanish Invention No. 200001654, which is formed by microorganisms between 3 and 5%, enzymes between 1 and 2%, algae marine between 46 and 55%, and clay between 38 and 50%.

Also in French Invention Patent No. 9215518 describes and claims a composition of matter for the chemical and physical chemical purification of aqueous liquids, contaminated, intended to be expanded in the liquid to be purified, comprising at least the following two materials in the form granular:

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a porous calcium carbonate, rich in oligo-elements, Y

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a Aluminum silicate.

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These two subjects return to the state of absorption of specific bacteria for biological degradation of organic carbon chain materials.

However, neither by composition nor results are they reach the decomposition values of organic matter desired, as will be seen later by the tests of Laboratory performed and described below.

The recommended compound is a compound of aerobic and anaerobic bacteria, necessary for metabolism Molecular of bacteria. Therefore, the compound of the invention acts as a biological activator that degrades by means enzymatic organic matter, helping to reduce ecological problems, reducing bad odors and accelerating the natural decomposition process, retaining materials highly polluting as heavy metals without letting it free the subsoil, thereby preserving the environment.

Once liquids are leached they enter contact with a bag containing the recommended compound, starting the decomposition process, the bacteria will multiply and secrete enzymes that digest matter organic In essence, a biological chain reaction begins that will continue until all the tissue has disappeared, when the compound of the invention as a biological activator that degrades by enzymatic means organic matter.

The composition of the bacteriological compound Enzymatic object of the invention comprises the following elements:

to)

Micro-organisms of the genus Bacillus (in a 5 to 15% weight ratio) of the type:

?

anaerobic bacteria, or

?

aerobic bacteria

b)

Enzymes (in a proportion by weight of 1 to 5%) of the type:

?

Amylases,

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proteases,

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lipases, and / or

?

cellulases

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C)

Absorbents of the bio-enzyme (in a weight ratio of 80 to 90%) of the type:

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acrylates,

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polyacrylates, and / or

?

Silicas

d)

Optionally others components:

?

activators, and / or

?

wetting agents

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Anaerobic bacteria are microorganisms necessary for the digestion of organic matter in the absence of air.

Aerobic bacteria are microorganisms necessary for the digestion of materials.

Preferably, the micro-organisms of the genus Bacillus will be of the species Bacillus Subtilis in a percentage comprised between 50 and 70% (preferably 60%) and of the species Bacillus Laterosporus in a percentage comprised between 30 and 50% ( preferably 40%).

Amylases act on the hydrates of carbon (starches), turning them into dextrins and sugars soluble.

Proteases have the function of converting  proteins in polypeptides and soluble amino acids.

The lipases, their action develops on the fats, converting them into soluble acids and glycerols.

Cellulases act on cellulose, turning it into soluble sugars.

The absorbent polycylamide has the function of absorb liquids, so that it enables an aqueous habitat optimal for bacterial development and enzymatic activity soluble.

Activators have the function of increasing the  Enzyme activity synergistically, causing the degeneration reactions are faster.

Wetting agents have the function of increase contact between enzyme and waste material, favoring a quick action.

Obtaining the bacteriological compound Enzymatic of the invention is achieved by a process of mix in a mixer called "V", of which are formed by two cylindrical arms equal and joined at an angle, of so that axial currents are created inside that separate and join the material to be mixed and that together with the action of its inner horizontal axis that rotates a series of shape blades independent, results in a fast and homogeneous mixing, which develops a process with smooth / solid smoothness and fluidity (in any percentage) and solids / liquids (maximum 10%), in the form of powder or granulate and with different specific weights.

The methodology for obtaining the compound Bacteriological enzyme of the invention is as follows: introduce the 3 or 4 component components of the compound Bacteriological enzymatic - that is, the bacteriological product, the Enzymatic product and absorbent product in the mixer type "V" in order of proportion, which will first be the absorbent product and enzymatic product (preferably with a weight ratio of 85%) and then the product bacteriological (preferably with a weight ratio of fifteen%).

Once the aforementioned components have been introduced in the mixer, it closes tightly through the vacuum application (to prevent component activation bacteriological sensitive to contact with air) and then it the constant and refrigerated mixing process begins by applying water to keep the temperature stable for a period not less than 30 minutes.

Once the mixture is finished, it will be download directly from the mixer type "V" lower than the hopper of the horizontal packaging machine that introduces the final product first in a bag water-soluble thermo-sealed and subsequently in a Polypropylene outer bag with light and air barrier in order to preserve the active component of the final product in latent state until use.

Other details and features will go away showing in the course of the description that to given below, in which reference is made to the details of The experimental results of three samples. These details are they give by way of illustration but not limitation of the invention.

The samples used in the different experiments carried out in order to check the advantageous properties of the enzymatic bacteriological compound of The invention is referred to as:

one-

absorbent product (product so only carries bio-enzyme absorbers);

2-

E-E product (compound bacteriological enzyme of the invention), obtained from the mixture in a "V" type mixer, of solids / solids and solids / liquids with a maximum 10% of the seconds, in the following proportions:

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\ vtcortauna Absorbent in a weight ratio of 80 to 90%.

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\ vtcortauna Micro-organisms in a weight ratio of 5 to 15%.

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\ vtcortauna Enzymes with a weight ratio of 1 to 5%; Y

3-

base enzyme product (product that contains the enzymatic product + bacteriological product), which it has a humidity of 3.62% with a minimum content of 0.29% of Fats in dry tissue.

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Figure 1 is a graph for the determination of the buffering capacity of two of the samples compared.

Figure 2 shows the results of the Determination of the specific hydrolytic activity of the product Enzymatic base obtained with an electrophonic gel.

Figure 3 is a corresponding histogram to the hydrolytic activity of the base enzyme product obtained with the aforementioned electrophonic gel.

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The trials carried out were the following:

a) Determination of water absorption capacity distilled

one

The determination of the absorption capacity of distilled water of the three samples was performed on the surface, adding the solvent slowly and finally decanting the excess of absorbed liquid. If we perform the 15 ml falcons tube determination, the formation of a jelly with greater absorption capacity (360) is observed at
400 ml / gr - from 50 to 60 liters per bag of 150 grams of the product EE).

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b) Determination of buffering capacity

Secondly the capacity was determined buffer of two of the three samples (product E-E and base enzyme product), since both enzymes (biological catalysts) such as bacteria usually be more active with neutral pH (physiological pH).

For this determination, a double titration with hydrochloric acid and with sodium hydroxide.

2

The results obtained from this test are they illustrate in the graph of figure 1, in which in ordinates the pH is read, while in abscissa the radicals are read [OH-] in two curves that join the coordinates: the curve (a) corresponds to the E-E product and curve (b) corresponds to the product enzymatic base.

As can be seen in figure 1, both curves have a great inclination in the PH corresponding to neutral PH (6-7), while  stabilize in basic PH (8-12). It means that, as desired, surprisingly the product E-E has no buffer capacity at neutral PHs, but instead it does have buffer capacity at basic PH. Another of the results obtained is that being both parallel curves and almost identical, it can be said that the absorbent does not affect said buffering capacity, as expected.

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c) Determination of enzymatic activity hydrolytic

As can be seen in figure 2, for To this end, an electrophoretic gel was used as a support for perform separation of the proteins appeared using 10 samples of a type protein, specifically the purified protein Bovine serum albumin (BSA) treated with other products (5 mg samples of BSA protein treated with 2 mg of product Enzymatic base, 2 mg of E-E product at temperatures indicated for a period of 1 hour).

The samples used were:

3

In all samples, the main band (which corresponds to the BSA protein) is found in the central part, and bands of lower molecular weights are also observed (it is say that they appear at the bottom) that correspond to the different proteins that appear because of hydrolysis.

These bands below the main band central corresponding to the BSA protein are denser in the lanes 6 to 10 with respect to lanes 1 to 5. Lanes 1 to 5 correspond to bands that do not contain the enzyme product base, while lanes 6 to 10 correspond to the bands which do contain said base enzyme product, see figure no. 2. This indicates that a specific hydrolytic activity occurs of the base enzyme product under mild temperature conditions, which is one of the desired characteristics for the product of the invention.

A histogram is illustrated in figure 3 corresponding to the hydrolytic activity obtained from the gel electrophoretic (if not indicated otherwise the temperature is 37 ° C), the activity listed in the histogram relative and in the abscissa the characteristics of the different Treated BSA protein samples that have been tested.

From the aforementioned figure 3 it is extracted that the BSA product samples treated (6-10) regarding of untreated BSA product samples (1-5) they are approximately double, which means that the product Enzymatic base exhibits moderate hydrolytic activity both at  low temperatures (0-4ºC) as at temperatures averages (30-40ºC), which as already indicated is one of the desired characteristics for the product of the invention.

Suitably described the present invention in correspondence with the transcribed data and essays, easy is understand that they may be introduced into the formulation of the compound, any modifications of detail deemed convenient, as long as the essence of the invention that remains is not altered summarized in the following claims.

Claims (3)

1. Enzymatic bacteriological compound accelerating the decomposition of organic matter from which they are formed by the addition of microorganisms, enzymes, and absorbents, characterized in that it comprises the following proportions of elements mixed by simple mixing:

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absorbents of the bio-enzyme in a weight ratio of 80 to 90% mix;

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micro-organisms of the genus Bacillus in a proportion by weight of 5 to 15% of the mixture, the micro-organisms of the species Bacillus Subtilis being in a percentage between 50 and 70%, and of the species Bacillus Laterosporus in a percentage between 30 and 50%;

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enzymes in a weight ratio of 1 5% of the mixture.

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2. Enzymatic bacteriological compound accelerating the decomposition of organic matter according to the first claim, characterized in that the bio-enzyme absorbers are of the type: acrylates, polycylates, and / or silica. 3. Enzymatic bacteriological compound accelerating the decomposition of organic matter according to the first claim, characterized in that the enzymes are of the type: amylases, proteases, lipases and / or cellulases.