ES2302623A1 - Use of ethereal oils e.g. dill (Anethum graveolns), anise (Pimpinella anisum L.), basil (Ocimum basilicum), cajeput (Melaleuca leucadendron L.) for killing and/or preventing mites - Google Patents

Use of ethereal oils e.g. dill (Anethum graveolns), anise (Pimpinella anisum L.), basil (Ocimum basilicum), cajeput (Melaleuca leucadendron L.) for killing and/or preventing mites Download PDF

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ES2302623A1
ES2302623A1 ES200602250A ES200602250A ES2302623A1 ES 2302623 A1 ES2302623 A1 ES 2302623A1 ES 200602250 A ES200602250 A ES 200602250A ES 200602250 A ES200602250 A ES 200602250A ES 2302623 A1 ES2302623 A1 ES 2302623A1
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officinalis
citrus
melaleuca
origanum
essential oil
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ES2302623B1 (en
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Marco Pacchioni
Isabelle Pacchioni
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PURESSENTIEL TM
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Isabelle Pacchioni
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08Magnoliopsida [dicotyledons]
    • A01N65/10Apiaceae or Umbelliferae [Carrot family], e.g. parsley, caraway, dill, lovage, fennel or snakebed
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/06Coniferophyta [gymnosperms], e.g. cypress
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08Magnoliopsida [dicotyledons]
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08Magnoliopsida [dicotyledons]
    • A01N65/22Lamiaceae or Labiatae [Mint family], e.g. thyme, rosemary, skullcap, selfheal, lavender, perilla, pennyroyal, peppermint or spearmint
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08Magnoliopsida [dicotyledons]
    • A01N65/24Lauraceae [Laurel family], e.g. laurel, avocado, sassafras, cinnamon or camphor
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08Magnoliopsida [dicotyledons]
    • A01N65/28Myrtaceae [Myrtle family], e.g. teatree or clove
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08Magnoliopsida [dicotyledons]
    • A01N65/36Rutaceae [Rue family], e.g. lime, orange, lemon, corktree or pricklyash

Abstract

Use of ethereal oils (I) e.g. dill ( Anethum graveol ens), anise ( Pimpinella anisum L.), basil ( Ocimum basilicum), bay St.Thomas ( Pimenta racemosa), rose wood ( Aniba rosaedoara A. variety ducks amazon), cajeput ( Melaleuca leucadendron L.), china cinnamon ( Cinnamomum cassia), Atlas cedar ( Cedrus altantica manetti) and citron ( Citrus limon) is claimed as mite killing and/or preventing product. Use of ethereal oils (I) such as dill ( Anethum graveol ens), anise ( Pimpinella anisum L.), basil ( Ocimum basilicum), bay St.Thomas ( Pimenta racemosa), rose wood ( Aniba rosaedoara A. variety ducks amazon), cajeput ( Melaleuca leucadendron L.), china cinnamon ( Cinnamomum cassia), Atlas cedar ( Cedrus altantica manetti), citron ( Citrus limon), caraway seed ( Cumimum cyminum L.), cypress ( Cupressus sempervirens L.), eucalyptus ( Eucalyptus globulus), fennel ( Foeniculum vulgare Mill. Var. Dulce), juniper ( Juniperus communis), ginger ( Zingiber officinalis), clove ( Eugenia caryophyllata), Lavender officinalis ( Lavendula angustifolia P. Miller), grosso lavender ( Lavendula angustifolia or Lavendula latifolia), mace ( Myristica frangrans Houttuyn), tangerine ( Citrus reticulata), fiercer marjoram ( Origanum marjorana ( Marjorana hotensis)), balm ( Melissa officinalis), ruff mint ( Mentha spicata linaeus), peppermint ( Mentha piperita), myrrh ( Commiphora myrrha), myrtles ( Melaleuca viridiflora Solander ex Gaeriner), orange ( Citrus sinensis L.), oregano ( Origanum herecleoticum L. syn. Origanum vulgare spp. viridae), parsley ( Petroselum sativum), small grain bigaradier ( Citrus aurantium L. spp aurantium), Scotch pine ( Pinus sylverstris L.), rosemary ( Rosmariuns officinalis), savory ( Satureia montana L.), sage officinalis ( Salvia officinalis L.), Feldthymian ( Thymus serpyllum L.), tea-tree ( Melaleuca alternifolia), genuine thymian ( Thymus vulgaris L.), flagrantly verbena ( Lippia citriodora) and winter green ( Gaultheria procumbens L.) is claimed as mite killing and/or preventing product. An independent claim is included for a mite killing and/or deterring formulation comprises (I) and a solvent. ACTIVITY : Acaricide. No biological data is given. MECHANISM OF ACTION : None given.

Description

Compuesto acarífugo y/o acaricida a base de aceites esenciales.A acarifuge and / or acaricide compound based on essential oils.

La presente invención se refiere a un compuesto que tiene efecto acarífugo y/o acaricida, en especial para la utilización en moquetas y/o en ropa de cama.The present invention relates to a compound which has an acarifugal and / or acaricidal effect, especially for the use on carpets and / or bedding.

La presente invención se refiere igualmente a la utilización como mínimo de un aceite esencial. Se conoce ya la utilización de aceites esenciales en el campo de la cosmética y de la higiene.The present invention also relates to the  use of at least one essential oil. It is already known use of essential oils in the field of cosmetics and hygiene

Por primera vez se ha descubierto que aceites esenciales, conocidos de forma clásica para la fabricación de productos cosméticos o de higiene, son eficaces para la producción de productos acarífugos y acaricidas.For the first time it has been discovered that oils essential, known in a classical way for the manufacture of Cosmetic or hygiene products are effective for production of acarifugal and acaricidal products.

La presente invención se refiere a la utilización del conjunto de aceites esenciales escogidos entre eneldo (Anethum graveolens), anís (Pimpinella anisum L.), albahaca (Ocimum basilicum), Bay Sant Thomas (Pimenta racemosa), Palo de Rosa (Aniba rosaeodora A. Ducke, var. Amazonien), Cayeputi (Melaleuca leucadendron L.), canela china (Cinnamomum cassia), cedro atlas (Cedrus atlantica Manetti), limón (Citrus limon), comino (Cuminum cyminum L.), ciprés (Cupressus sempervirens L.), eucalipto (Eucalyptus globulus), hinojo (Foeniculum vulgare Mill: Var. dulce), enebro (Juniperus communis), jengibre (Zingiber officinalis), clavo (Eugenia caryophyllata), lavanda oficinal (lavandula angustifolia P. Miller), lavandín grosso (lavandula angustifolia x lavandula latifolia), macis (Myristica frangrans Houttuyn), mandarina (Citrus reticulata), mejorana salvaje (Origanum marjorana (Marjorana hortensis), melisa (Melissa officinalis), menta rizada (Mentha spicata linaeus), menta piperita (Mentha piperita), mirra (Commiphora myrrha), niaouli (Melaleuca viridiflora Solander ex Gaériner), naranja (Citrus sinensis L.), orégano (Origanum heracleoticum L. Syn. Origanum vulgare ssp. viride), perejil (Petroselum sativum), naranjas amargas (Citrus aurantium L ssp aurantium), pino silvestre (Pinus Sylvestris L.), romero (Rosmarinus officinalis), "sariete" de montaña (Satureia montana L.), salvia oficinal (Salvia officinalis L.), tomillo (thymus serpyllum L.), árbol de té (Melaleuca alternifolia), timo timol (Thymus vulgaris L.), verbena olorosa (Lippia citriodora), wintergreen (Gaultheria procumbens L.) para la fabricación de un producto acaricida y/o acarífugo.The present invention relates to the use of the set of essential oils chosen from dill ( Anethum graveolens ), anise ( Pimpinella anisum L. ), basil ( Ocimum basilicum ), Bay Sant Thomas ( Pimenta racemosa ), Rosewood ( Aniba rosaeodora A Ducke, var. Amazonien ), Cayeputi ( Melaleuca leucadendron L. ), Chinese cinnamon ( Cinnamomum cassia ), Cedar atlas ( Cedrus atlantica Manetti ), Lemon ( Citrus limon ), Cumin ( Cuminum cyminum L. ), Cypress ( Cupressus sempervirens L . ), eucalyptus ( Eucalyptus globulus ), fennel ( Foeniculum vulgare Mill: Var. dulce ), juniper ( Juniperus communis ), ginger ( Zingiber officinalis ), clove ( Eugenia caryophyllata ), officinal lavender ( Lavandula angustifolia P. Miller ), lavandín grosso ( lavandula angustifolia x lavandula latifolia ), mace ( Myristica frangrans Houttuyn ), tangerine ( Citrus reticulata ), wild marjoram ( Origanum marjorana ( Marjorana hortensis ), melissa ( Melissa officinalis ), curly mint ( Menth a spicata linaeus ), peppermint ( Mentha piperita ), myrrh ( Commiphora myrrha ), niaouli ( Melaleuca viridiflora Solander ex Gaériner ), orange ( Citrus sinensis L. ), oregano ( Origanum heracleoticum L. Syn. Origanum vulgare ssp. viride ), parsley ( Petroselum sativum ), bitter oranges ( Citrus aurantium L ssp aurantium ), wild pine ( Pinus Sylvestris L. ), rosemary ( Rosmarinus officinalis ), mountain sariete ( Satureia montana L. ), office sage ( Sage officinalis L. ), thyme ( thymus serpyllum L. ), tea tree ( Melaleuca alternifolia ), thymus thymol ( Thymus vulgaris L. ), verbena olorosa ( Lippia citriodora ), wintergreen ( Gaultheria procumbens L. ) for the manufacture of a product acaricide and / or acarifuge.

La presente invención se refiere igualmente a un compuesto acaricida y/o acarífugo caracterizado por comprender el conjunto de aceites esenciales escogidos entre el eneldo (Anethum graveolens), anís (Pimpinella anisum L.), albahaca (Ocimum basilicum), Bay Sant Thomas (Pimenta racemosa), Palo de Rosa (Aniba rosaeodora A. Ducke, var. Amazonien), Cayeputi (Melaleuca leucadendron L.), canela china (Cinnamomum cassia), cedro atlas (Cedrus atlantica Manetti), limón (Citrus limon), comino (Cuminum cyminum L.), ciprés (Cupressus sempervirens L.), eucalipto (Eucalyptus globulus), hinojo (Foeniculum vulgare Mill: Var. dulce), enebro (Juniperus communis), jengibre (Zingiber officinalis), clavo (Eugenia caryophyllata), lavanda oficinal (lavandula angustifolia P. Miller), lavandín grosso (lavandula angustifolia x lavandula latifolia), macis (Myristica frangrans Houttuyn), mandarina (Citrus reticulata), mejorana salvaje (Origanum marjorana (Marjorana hortensis), melisa (Melissa officinalis), menta rizada (Mentha spicata linaeus), menta piperita (Mentha piperita), mirra (Commiphora myrrha), niaouli (Melaleuca viridiflora Solander ex Gaériner), naranja (Citrus sinensis L.), orégano (Origanum heracleoticum L. Syn. Origanum vulgare ssp. viride), perejil (Petroselum sativum), naranjas amargas (Citrus aurantium L ssp aurantium), pino silvestre (Pinus Sylvestris L.), romero (Rosmarinus officinalis), "sariete" de montaña (Satureia montana L.), salvia oficinal (Salvia officinalis L.), tomillo (thymus serpyllum L.), árbol de té (Melaleuca alternifolia), timo timol (Thymus vulgaris L.), verbena olorosa (Lippia citriodora), wintergreen (Gaultheria procumbens L.) y un disolvente.The present invention also relates to an acaricidal and / or acarifuge compound characterized by comprising the set of essential oils chosen from dill ( Anethum graveolens ), anise ( Pimpinella anisum L. ), basil ( Ocimum basilicum ), Bay Sant Thomas ( Pimenta racemosa ), Rosewood ( Aniba rosaeodora A. Ducke, var. Amazonien ), Cayeputi ( Melaleuca leucadendron L. ), Chinese cinnamon ( Cinnamomum cassia ), Atlas cedar ( Cedrus atlantica Manetti ), Lemon ( Citrus limon ), Cumin ( Cuminum cyminum L. ), cypress ( Cupressus sempervirens L. ), eucalyptus ( Eucalyptus globulus ), fennel ( Foeniculum vulgare Mill: Var. dulce ), juniper ( Juniperus communis ), ginger ( Zingiber officinalis ), clove ( Eugenia caryophyllata ), lavender officinal ( Lavandula angustifolia P. Miller ), lavandín grosso ( lavandula angustifolia x lavandula latifolia ), mace ( Myristica frangrans Houttuyn ), tangerine ( Citrus reticulata ), wild marjoram ( Origanum marjorana ( Marjorana h ortensis ), melissa ( Melissa officinalis ), curly mint ( Mentha spicata linaeus ), mint piperita ( Mentha piperita ), myrrh ( Commiphora myrrha ), niaouli ( Melaleuca viridiflora Solander ex Gaériner ), orange ( Citrus sinensis L. ) oregano ( Orig heracleoticum L. Syn. Origanum vulgare ssp. viride ), parsley ( Petroselum sativum ), bitter oranges ( Citrus aurantium L ssp aurantium ), wild pine ( Pinus Sylvestris L. ), rosemary ( Rosmarinus officinalis ), mountain sariete ( Satureia montana L. ), office sage ( Sage officinalis L. ), thyme ( thymus serpyllum L. ), tea tree ( Melaleuca alternifolia ), thymus thymol ( Thymus vulgaris L. ), verbena olorosa ( Lippia citriodora ), wintergreen ( Gaultheria procumbens L. ) and a solvent.

Según un modo de realización preferente de la invención, el compuesto presenta igualmente aceite esencial de toronjil (Cymbopogon winterianus Jowitt).According to a preferred embodiment of the invention, the compound also has melissa essential oil ( Cymbopogon winterianus Jowitt ).

Según otra forma de realización preferente de la invención el compuesto comporta igualmente aceite esencial de geranio (Pelargonium graveolens).According to another preferred embodiment of the invention, the compound also contains geranium essential oil ( Pelargonium graveolens ).

Según un perfeccionamiento de la invención el disolvente es un alcohol ftalado, especialmente al 96%.According to an improvement of the invention the Solvent is a phthalated alcohol, especially 96%.

Según una forma de realización preferente de la invención, el disolvente representa de 50 a 90% en peso del compuesto especialmente el 75%.According to a preferred embodiment of the invention, the solvent represents 50 to 90% by weight of the Composed especially 75%.

Se ha estudiado el efecto acarífugo y/o acaricida de un compuesto según la invención, indicado a título de ejemplo, en una cepa de acarios "Dermatophagoides Pteronyssinus", que viven en las moquetas y en la ropa de cama. El producto a comprobar llamado PURESSENTIEL Spray Assainissant con 41 aceites esenciales (marcas registradas) presenta 41 aceites esenciales, a saber eneldo (Anethum graveolens), anís (Pimpinella anisum L.), albahaca (Ocimum basilicum), Bay Sant Thomas (Pimenta racemosa), Palo de Rosa (Aniba rosaeodora A. Ducke, var. Amazonien), Cayeputi (Melaleuca leucadendron L.), canela china (Cinnamomum cassia), cedro atlas (Cedrus atlantica Manetti), limón (Citrus limon), comino (Cuminum cyminum L.), ciprés (Cupressus sempervirens L.), eucalipto (Eucalyptus globulus), hinojo (Foeniculum vulgare Mill: Var. dulce), enebro (Juniperus communis), jengibre (Zingiber officinalis), clavo (Eugenia caryophyllata), lavanda oficinal (lavandula angustifolia P. Miller), lavandín grosso (lavandula angustifolia x lavandula latifolia), macis (Myristica frangrans Houttuyn), mandarina (Citrus reticulata), mejorana salvaje (Origanum marjorana (Marjorana hortensis), melisa (Melissa officinalis), menta rizada (Mentha spicata linaeus), menta piperita (Mentha piperita), mirra (Commiphora myrrha), niaouli (Melaleuca viridiflora Solander ex Gaériner), naranja (Citrus sinensis L.), orégano (Origanum heracleoticum L. Syn. Origanum vulgare ssp. viride), perejil (Petroselum sativum), naranjas amargas (Citrus aurantium L ssp aurantium), pino silvestre (Pinus Sylvestris L.), romero (Rosmarinus officinalis), "sariete" de montaña (Satureia montana L.), salvia oficinal (Salvia officinalis L.), tomillo (thymus serpyllum L.), árbol de té (Melaleuca alternifolia), timo timol (Thymus vulgaris L.), verbena olorosa (Lippia citriodora), wintergreen (Gaultheria procumbens L.), toronjil (Cymbopogon winterianus Jowitt) y el geranio (Pelargonium graveolens), (ver anexo C). Se ha comprobado en comparación con un producto aceitoso que sirve de producto testigo. Este producto aceitoso está realizado a base de aceite de
colza.
The acarifugal and / or acaricidal effect of a compound according to the invention, indicated by way of example, in a strain of " Dermatophagoides Pteronyssinus ", which live on carpets and bedding, has been studied. The product to be tested called PURESSENTIEL Spray Assainissant with 41 essential oils (trademarks) presents 41 essential oils, namely dill ( Anethum graveolens ), anise ( Pimpinella anisum L. ), basil ( Ocimum basilicum ), Bay Sant Thomas ( Pimenta racemosa ) , Rosewood ( Aniba rosaeodora A. Ducke, var. Amazonien ), Cayeputi ( Melaleuca leucadendron L. ), Chinese cinnamon ( Cinnamomum cassia ), Cedar atlas ( Cedrus atlantica Manetti ), Lemon ( Citrus limon ), Cumin ( Cuminum cyminum L . ), cypress ( Cupressus sempervirens L. ), eucalyptus ( Eucalyptus globulus ), fennel ( Foeniculum vulgare Mill: Var. dulce ), juniper ( Juniperus communis ), ginger ( Zingiber officinalis ), clove ( Eugenia caryophyllata ), officinal lavender ( lavandula angustifolia P. Miller ), lavandin grosso ( lavandula angustifolia x lavandula latifolia ), mace ( Myristica frangrans Houttuyn ), tangerine ( Citrus reticulata ), wild marjoram ( Origanum marjorana ( Marjorana hortensis ), meli sa ( Melissa officinalis ), curly mint ( Mentha spicata linaeus ), mint piperita ( Mentha piperita ), myrrh ( Commiphora myrrha ), niaouli ( Melaleuca viridiflora Solander ex Gaériner ), orange ( Citrus sinensis L. ), oregano ( Origanum heracleoticum L. Syn. Origanum vulgare ssp. viride ), parsley ( Petroselum sativum ), bitter oranges ( Citrus aurantium L ssp aurantium ), wild pine ( Pinus Sylvestris L. ), rosemary ( Rosmarinus officinalis ), mountain "sariete" ( Satureia montana L. ), office sage ( Sage officinalis L. ), thyme ( thymus serpyllum L. ), tea tree ( Melaleuca alternifolia ), thymus thymol ( Thymus vulgaris L. ), verbena olorosa ( Lippia citriodora ), wintergreen ( Gaultheria procumbens L. ), melissa ( Cymbopogon winterianus Jowitt ) and geranium ( Pelargonium graveolens ), (see annex C). It has been proven in comparison to an oily product that serves as a control product. This oily product is made from oil of
rape.

Treinta ácaros han sido depositados en dos cajas de Pétri, a razón de 15 por caja, para cada uno de los productos objetos de pruebas y del producto testigo. A continuación se han puesto en presencia del producto a comprobar, el cual estaba colocado en medio de papel de filtro.Thirty mites have been deposited in two boxes  Pétri, at a rate of 15 per box, for each of the products test objects and the witness product. Then they have put in the presence of the product to be checked, which was placed in the middle of filter paper.

Los contajes de las migraciones y mortalidades eventuales para las muestras comprobadas números 1 y 2, han sido efectuadas a los 7 minutos 30 segundos, 15 minutos, 30 minutos y después a 1, 2, 3, 4 y 6 horas después de contacto de los ácaros con el producto.The counts of migrations and mortalities Any samples for numbers 1 and 2 have been tested. made at 7 minutes 30 seconds, 15 minutes, 30 minutes and then at 1, 2, 3, 4 and 6 hours after mite contact with the product.

Los ensayos realizados sobre el producto PURESSENTIEL Spray Assainissant con 41 aceites esenciales (marcas registradas) llevan a la conclusión de que éste es perfectamente acarífugo y acaricida.The tests performed on the product PURESSENTIEL Spray Assainissant with 41 essential oils (brands registered) lead to the conclusion that this is perfectly acarifuge and acaricide.

En lo que respecta al efecto acarífugo, el cual se obtiene desde los 30 minutos, el conjunto de los ácaros ha migrado. Esta migración era del orden del 66% desde la primera observación. En la segunda, se ha contado la migración de 26 ácaros sobre 30. Por otra parte, las mortalidades eran de 10 a los 7 minutos 30 segundos, es decir 33%. A los 15 minutos, el número de mortalidad era de 18. Se han obtenido 27 mortalidades a los 30 minutos, 29 a una hora y 30 a las dos horas. Estos resultados demuestran la excelente actividad acarífuga y acaricida del producto a comprobar PURESSENTIEL Spray Assainissant con 41 aceites esenciales (marcas registradas).Regarding the acarifuge effect, which obtained from 30 minutes, the whole of the mites has my degree. This migration was of the order of 66% from the first observation. In the second, the migration of 26 mites has been counted over 30. On the other hand, the mortalities were from 10 to 7 minutes 30 seconds, that is 33%. At 15 minutes, the number of mortality was 18. There have been 27 mortalities at 30 minutes, 29 at one hour and 30 at two hours. This results demonstrate the excellent acarifugal and acaricidal activity of product to check PURESSENTIEL Spray Assainissant with 41 oils essential (trademarks).

Por su parte el producto testigo no ha inducido ninguna respuesta significativa. El número de las migraciones es especialmente reducido.For its part, the control product has not induced No meaningful response. The number of migrations is especially small

Éste ha sido de uno para el primer tiempo de lectura y después de cinco a los 15 minutos, llegando a 6 en una hora. El número de migraciones total es de 10 a las 6 horas, que es el último tiempo de observación. Este número de migraciones es particularmente reducido y no permite llegar a la conclusión de que tenga un efecto acarífugo. Finalmente, este producto no ha provocado mortalidad alguna durante este estudio, no habiéndose podido comprobar ningún efecto acaricida.This has been one for the first time of reading and after five at 15 minutes, reaching 6 in one hour. The total number of migrations is from 10 to 6 hours, which is The last time of observation. This number of migrations is particularly reduced and does not allow to conclude that Have a acarifugal effect. Finally, this product has not caused any mortality during this study, not having Was able to check any acaricidal effect.

Se ha obtenido un efecto acaricida y acarífugo con el producto PURESSENTIEL Spray Assainissant con 41 aceites esenciales (marcas registradas). Este efecto debe ser considerado muy fuerte en la medida en la que los resultados obtenidos lo han sido durante la primera fase del estudio.A acaricidal and acarifuge effect has been obtained with the product PURESSENTIEL Spray Assainissant with 41 oils essential (trademarks). This effect must be considered. very strong to the extent that the results obtained have It was during the first phase of the study.

Material y métodosMaterial and methods 1. Productos a comprobar1. Products to check

El producto a comprobar, PURESSENTIEL Spray Assainissant con 41 aceites esenciales (marcas registradas), lote 051744, presentado en forma de líquido de color amarillo transparente, ha sido enviado en su envase. La prueba se ha referido a las formulaciones siguientes:The product to check, PURESSENTIEL Spray Assainissant with 41 essential oils (registered trademarks), lot 051744, presented in the form of a yellow liquid transparent, has been sent in its container. The test has been referred to the following formulations:

- PURESSENTIEL Spray Assainissant con 41 aceites esenciales (marcas registradas) conteniendo los 41 aceites esenciales.- PURESSENTIEL Spray Assainissant with 41 oils essentials (trademarks) containing the 41 oils essential.

- Un producto aceitoso como producto testigo.- An oily product as a product witness.

El producto a comprobar PURESSENTIEL Spray Assainissant con 41 aceites esenciales (marcas registradas) ha sido utilizado con la concentración siguiente (% en peso):The product to check PURESSENTIEL Spray Assainissant with 41 essential oils (registered trademarks) has been used with the following concentration (% by weight):

Alcohol ftalano 96%Phthalane alcohol 96%
75,00075,000

Mentol natural cristalizadoNatural menthol crystallized
0,2800.280

Aceite esencial Menta PiperitaMint essential oil Piperita
0,7100.710

Aceite esencial Menta RizadaMint essential oil Curly
0,4600.460

Aceite esencial AnísEssential oil Anise
0,1100,110

Aceite esencial HinojoEssential oil Fennel
0,1000.100

Aceite esencial RomeroEssential oil Rosemary
0,6800.680

Aceite esencial CominoEssential oil Cumin
0,0100.010

Aceite esencial TimoEssential oil Scam
0,2400.240

Aceite esencial Canela ChinaCinnamon essential oil China
0,0500.050

Aceite esencial ClavoEssential oil Nail
0,1300,130

Aceite esencial WintergreenEssential oil Wintergreen
0,6000.600

Aceite esencial EnebroEssential oil Juniper
0,0500.050

Aceite esencial EneldoEssential oil Dill
0,1000.100

Aceite esencial NaranjaEssential oil Orange
1,4001,400

Aceite esencial LavandaEssential oil Lavender
5,6805,680

Aceite esencial Limón FABLemon essential oil Fab
1,1701,170

Aceite esencial CayeputiEssential oil Cayeputi
0,6800.680

Aceite esencial EucaliptoEssential oil Eucalyptus
7,5507,550

Aceite esencial ToronjilEssential oil Melissa
0,1000.100

Aceite esencial PinoEssential oil Pine tree
1,6951,695

Aceite esencial Salvia OficinalSage essential oil Office
0,0400.040

Aceite esencial Palo de RosaEssential oil Stick pink
0,0400.040

Aceite esencial MacisEssential oil Mace
0,0250.025

Aceite esencial JengibreEssential oil Ginger
0,0200.020

Aceite esencial MelisaEssential oil Balm
0,0470.047

Aceite esencial MirraEssential oil Myrrh
0,0100.010

Aceite esencial LavandaEssential oil Lavender
0,1350.135

Aceite esencial NiaouliEssential oil Niaouli
1,7351,735

Aceite esencial Petit GrainPetit essential oil Grain
0,2750.275

Aceite esencial AjedreaEssential oil Savory
0,0500.050

Aceite esencial TomilloEssential oil Thyme
0,0200.020

Aceite esencial GeranioEssential oil Geranium
0,0500.050

Aceite esencial MejoranaEssential oil Marjoram
0,0100.010

Aceite esencial OréganoEssential oil Oregano
0,0500.050

Aceite esencial CiprésEssential oil Cypress
0,2200.220

Aceite esencial AlbahacaEssential oil Basil
0,0400.040

Aceite esencial PerejilEssential oil Parsley
0,0200.020

Aceite esencial Árbol del TéEssential Oil Tree Tea
0,0900.090

Aceite esencial Bay St. ThomasBay St. essential oil Thomas
0,0800.080

Aceite esencial VerbenaEssential oil Verbena
0,0030.003

Aceite esencial MandarinaEssential oil Tangerine
0,1150.115

Aceite esencial CedroEssential oil Cedar
0,1300,130

Las cantidades de productos utilizados han sido aproximadamente de 1,5 g.The quantities of products used have been approximately 1.5 g.

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2. Ácaros2. Mites 2.1 Ácaros a comprobar2.1 Mites to check

Treinta ácaros Dermatophagoides pteronyssinus han sido objeto de comprobación en el curso de este estudio para cada formulación, es decir un total de 150 ácaros. Estos provenían de recogida sobre moquetas, medio en el que estos ácaros se desarrollan particularmente. Estos ácaros han sido conservados en cajas Pétri durante 96 horas. Se han colocado 15 ácaros en una caja Pétri cuadrada de 12,5 cm de lado y 1,5 cm de altura. Se han utilizado dos cajas para esta prueba y por cada muestra. El fondo de las cajas estaba tapizado por un papel de filtro.Thirty Dermatophagoides pteronyssinus mites have been tested in the course of this study for each formulation, that is, a total of 150 mites. These came from collection on carpets, medium in which these mites develop particularly. These mites have been preserved in Pétri boxes for 96 hours. 15 mites have been placed in a square Pétri box 12.5 cm side and 1.5 cm high. Two boxes have been used for this test and for each sample. The bottom of the boxes was upholstered by a filter paper.

Se ha trazado círculos concéntricos en número de cuatro y separados en 1,5 cm cada uno de ellos utilizando el centro de la caja como punto central.Concentric circles in number of four and separated by 1.5 cm each using the center of the box as a central point.

Se ha depositado un volumen de 1,0 g de producto en el círculo más pequeño y a continuación los ácaros, en número de quince, han sido depositados en el centro de la caja. El número de las migraciones, así como las mortalidades eventuales se han contado y aparecen en el anexo del informe.A volume of 1.0 g of product has been deposited  in the smallest circle and then the mites, in number of fifteen, they have been deposited in the center of the box. The number of migrations, as well as eventual mortalities have counted and appear in the annex to the report.

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3. Observaciones3. Observations

El contaje de las migraciones y mortalidades ha sido efectuado a los 7,30 minutos, 15 minutos, 30 minutos, 1 hora, 2 horas, 3 horas, 4 horas y 6 horas después de introducción de la cepa en las cajas de experimentación.The counting of migrations and mortalities has been done at 7.30 minutes, 15 minutes, 30 minutes, 1 hour, 2 hours, 3 hours, 4 hours and 6 hours after the introduction of the strain in the experiment boxes.

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Anexo AAnnexed TO

1one

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Anexo BAnnexed B

22

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Anexo CAnnexed C

33

44

55

Claims (9)

1. Utilización del conjunto de aceites esenciales siguientes: eneldo (Anethum graveolens), anís (Pimpinella anisum L.), albahaca (Ocimum basilicum), Bay Sant Thomas (Pimenta racemosa), Palo de Rosa (Aniba rosaeodora A. Ducke, var. Amazonien), Cayeputi (Melaleuca leucadendron L.), canela china (Cinnamomum cassia), cedro atlas (Cedrus atlantica Manetti), limón (Citrus limon), comino (Cuminum cyminum L.), ciprés (Cupressus sempervirens L.), eucalipto (Eucalyptus globulus), hinojo (Foeniculum vulgare Mill: Var. dulce), enebro (Juniperus communis), jengibre (Zingiber officinalis), clavo (Eugenia caryophyllata), lavanda oficinal (lavandula angustifolia P. Miller), lavandín grosso (lavandula angustifolia x lavandula latifolia), macis (Myristica frangrans Houttuyn), mandarina (Citrus reticulata), mejorana salvaje (Origanum marjorana (Marjorana hortensis), melisa (Melissa officinalis), menta rizada (Mentha spicata linaeus), menta piperita (Mentha piperita), mirra (Commiphora myrrha), niaouli (Melaleuca viridiflora Solander ex Gaériner), naranja (Citrus sinensis L.), orégano (Origanum heracleoticum L. Syn. Origanum vulgare ssp. viride), perejil (Petroselum sativum), naranjas amargas (Citrus aurantium L ssp aurantium), pino silvestre (Pinus Sylvestris L.), romero (Rosmarinus officinalis), "sariete" de montaña (Satureia montana L.), salvia oficinal (Salvia officinalis L.), tomillo (thymus serpyllum L.), árbol de té (Melaleuca alternifolia), timo timol (Thymus vulgaris L.), verbena olorosa (Lippia citriodora), wintergreen (Gaultheria procumbens L.) como producto acaricida y/o acarífugo.1. Use of the following essential oils set: dill ( Anethum graveolens ), anise ( Pimpinella anisum L. ), basil ( Ocimum basilicum ), Bay Sant Thomas ( Pimenta racemosa ), Rosewood ( Aniba rosaeodora A. Ducke, var. Amazonien ), Cayeputi ( Melaleuca leucadendron L. ), Chinese cinnamon ( Cinnamomum cassia ), Cedar atlas ( Cedrus atlantica Manetti ), Lemon ( Citrus limon ), Cumin ( Cuminum cyminum L. ), Cypress ( Cupressus sempervirens L. ), Eucalyptus ( Eucalyptus globulus ), fennel ( Foeniculum vulgare Mill: Var. Dulce ), juniper ( Juniperus communis ), ginger ( Zingiber officinalis ), clove ( Eugenia caryophyllata ), officinal lavender ( lavandula angustifolia P. Miller ), lavandin grosso ( lavandula angustifolia x lavandula angustifolia x lavandula latifolia ), mace ( Myristica frangrans Houttuyn ), tangerine ( Citrus reticulata ), wild marjoram ( Origanum marjorana ( Marjorana hortensis ), melissa ( Melissa officinalis ), curly mint ( Mentha spicata linaeus ), peppermint ( Me ntha piperita ), myrrh ( Commiphora myrrha ), niaouli ( Melaleuca viridiflora Solander ex Gaériner ), orange ( Citrus sinensis L. ), oregano ( Origanum heracleoticum L. Syn. Origanum vulgare ssp. viride ), parsley ( Petroselum sativum ), bitter oranges ( Citrus aurantium L ssp aurantium ), wild pine ( Pinus Sylvestris L. ), rosemary ( Rosmarinus officinalis ), mountain sariete ( Satureia montana L. ), office sage ( Sage officinalis L. ), thyme ( thymus serpyllum L. ), tea tree ( Melaleuca alternifolia ), thymus thymol ( Thymus vulgaris L. ), verbena olorosa ( Lippia citriodora ), wintergreen ( Gaultheria procumbens L. ) as acaricidal product and / or acarifuge 2. Utilización, según la reivindicación 1, en la que se utiliza igualmente el geranio (Pelargonium graveolens).2. Use according to claim 1, wherein the geranium ( Pelargonium graveolens ) is also used. 3. Utilización, según una de las reivindicaciones 1 a 2, en la que se utiliza igualmente aceite esencial de toronjil (Cymbopogon winterianus Jowitt).3. Use according to one of claims 1 to 2, in which melissa essential oil ( Cymbopogon winterianus Jowitt ) is also used. 4. Compuesto acaricida y/o acarífugo caracterizado por comprender el conjunto de aceites esenciales siguientes: eneldo (Anethum graveolens), anís (Pimpinella anisum L.), albahaca (Ocimum basilicum), Bay Sant Thomas (Pimenta racemosa), Palo de Rosa (Aniba rosaeodora A. Ducks, var. Amazonien), Cayeputi (Melaleuca leucadendron L.), canela china (Cinnamomum cassia), cedro atlas (Cedrus atlantica Manetti), limón (Citrus limon), comino (Cuminum cyminum L.), ciprés (Cupressus sempervirens L.), eucalipto (Eucalyptus globulus), hinojo (Foeniculum vulgare Mill: Var. dulce), enebro (Juniperus communis), jengibre (Zingiber officinalis), clavo (Eugenia caryophyllata), lavanda oficinal (lavandula angustifolia P. Miller), lavandín grosso (lavandula angustifolia x lavandula latifolia), macis (Myristica frangrans Houttuyn), mandarina (Citrus reticulata), mejorana salvaje (Origanum marjorana (Marjorana hortensis), melisa (Melissa officinalis), menta rizada (Mentha spicata linaeus), menta piperita (Mentha piperita), mirra (Commiphora myrrha), niaouli (Melaleuca viridiflora Solander ex Gaériner), naranja (Citrus sinensis L.), orégano (Origanum heracleoticum L. Syn. Origanum vulgare ssp. viride), perejil (Petroselum sativum), naranjas amargas (Citrus aurantium L ssp aurantium), pino silvestre (Pinus Sylvestris L.), romero (Rosmarinus officinalis), "sariete" de montaña (Satureia montana L.), salvia oficinal (Salvia officinalis L.), tomillo (thymus serpyllum L.), árbol de té (Melaleuca alternifolia), timo timol (Thymus vulgaris L.), verbena olorosa (Lippia citriodora), wintergreen (Gaultheria procumbens L.) y un disolvente.4. Acaricidal and / or acarifuge compound characterized by comprising the following set of essential oils: dill ( Anethum graveolens ), anise ( Pimpinella anisum L. ), basil ( Ocimum basilicum ), Bay Sant Thomas ( Pimenta racemosa ), Palo de Rosa ( Aniba rosaeodora A. Ducks, var. Amazonien ), Cayeputi ( Melaleuca leucadendron L. ), Chinese cinnamon ( Cinnamomum cassia ), Cedar atlas ( Cedrus atlantica Manetti ), Lemon ( Citrus lemon ), Cumin ( Cuminum cyminum L. ), Cypress ( Cupressus sempervirens L. ), eucalyptus ( Eucalyptus globulus ), fennel ( Foeniculum vulgare Mill: Var. Dulce ), juniper ( Juniperus communis ), ginger ( Zingiber officinalis ), clove ( Eugenia caryophyllata ), lavender officinal ( Lavandula angustifolia P. Miller ) , lavandín grosso ( lavandula angustifolia x lavandula latifolia ), mace ( Myristica frangrans Houttuyn ), tangerine ( Citrus reticulata ), wild marjoram ( Origanum marjorana ( Marjorana hortensis ), melissa ( Melissa officinalis ), mint riza da ( Mentha spicata linaeus ), peppermint ( Mentha piperita ), myrrh ( Commiphora myrrha ), niaouli ( Melaleuca viridiflora Solander ex Gaériner ), orange ( Citrus sinensis L. ), oregano ( Origanum heracleoticum L. Syn. Origanum vulgare ssp. viride ), parsley ( Petroselum sativum ), bitter oranges ( Citrus aurantium L ssp aurantium ), wild pine ( Pinus Sylvestris L. ), rosemary ( Rosmarinus officinalis ), mountain sariete ( Satureia montana L. ), office sage ( Sage officinalis L. ), thyme ( thymus serpyllum L. ), tea tree ( Melaleuca alternifolia ), thymus thymol ( Thymus vulgaris L. ), verbena olorosa ( Lippia citriodora ), wintergreen ( Gaultheria procumbens L. ) and a solvent. 5. Compuesto, según la reivindicación 4, caracterizado por comportar igualmente aceite esencial de geranio (Pelargonium graveolens).5. A compound according to claim 4, characterized in that it also includes geranium essential oil ( Pelargonium graveolens ). 6. Compuesto, según la reivindicación 4 ó 5, caracterizado por comportar igualmente aceite esencial de toronjil (Cymbopogon winterianus Jowitt).6. A compound according to claim 4 or 5, characterized in that it also has melissa essential oil ( Cymbopogon winterianus Jowitt ). 7. Compuesto, según una de las reivindicaciones 4 a 6, caracterizado por comprender todos los aceites esenciales mencionados en las reivindicaciones 5 y 6.7. A compound according to one of claims 4 to 6, characterized in that it comprises all the essential oils mentioned in claims 5 and 6. 8. Compuesto, según una de las reivindicaciones 4 a 7, caracterizado porque el disolvente es un alcohol ftalado especialmente al 96%.8. Compound according to one of claims 4 to 7, characterized in that the solvent is a 96% phthalated alcohol. 9. Compuesto, según una de las reivindicaciones 4 a 8, caracterizado porque el disolvente representa entre 50 y 90% en peso del compuesto, especialmente 75%.9. A compound according to one of claims 4 to 8, characterized in that the solvent represents between 50 and 90% by weight of the compound, especially 75%.
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