ES2231030B1 - THERMRESISTOMETER FOR MEASURING THE HEAT RESISTANCE OF MICROORGANISMS IN TEMPERATURE CONTROLLED CONDITIONS, ABLE TO SIMULATE ISOTHERMAL AND NON-ISOTHERMAL TREATMENT CONDITIONS. - Google Patents
THERMRESISTOMETER FOR MEASURING THE HEAT RESISTANCE OF MICROORGANISMS IN TEMPERATURE CONTROLLED CONDITIONS, ABLE TO SIMULATE ISOTHERMAL AND NON-ISOTHERMAL TREATMENT CONDITIONS.Info
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- ES2231030B1 ES2231030B1 ES200302529A ES200302529A ES2231030B1 ES 2231030 B1 ES2231030 B1 ES 2231030B1 ES 200302529 A ES200302529 A ES 200302529A ES 200302529 A ES200302529 A ES 200302529A ES 2231030 B1 ES2231030 B1 ES 2231030B1
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- isothermal
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- heat resistance
- thermometer
- resistometer
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- 244000005700 microbiome Species 0.000 title claims description 22
- 238000011282 treatment Methods 0.000 title claims description 19
- 238000010438 heat treatment Methods 0.000 claims abstract description 25
- 238000001816 cooling Methods 0.000 claims abstract description 13
- 238000000034 method Methods 0.000 claims description 15
- 235000013305 food Nutrition 0.000 claims description 10
- 238000005259 measurement Methods 0.000 claims description 8
- 239000000523 sample Substances 0.000 claims description 8
- 230000001105 regulatory effect Effects 0.000 claims description 5
- 150000001875 compounds Chemical class 0.000 claims description 4
- 239000012530 fluid Substances 0.000 claims description 2
- 238000004088 simulation Methods 0.000 claims description 2
- 239000000126 substance Substances 0.000 claims description 2
- 238000011109 contamination Methods 0.000 claims 2
- 102000004190 Enzymes Human genes 0.000 claims 1
- 108090000790 Enzymes Proteins 0.000 claims 1
- 230000004075 alteration Effects 0.000 claims 1
- 239000003242 anti bacterial agent Substances 0.000 claims 1
- 229940088710 antibiotic agent Drugs 0.000 claims 1
- 230000005540 biological transmission Effects 0.000 claims 1
- 238000000855 fermentation Methods 0.000 claims 1
- 230000004151 fermentation Effects 0.000 claims 1
- 230000002779 inactivation Effects 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 claims 1
- 238000010200 validation analysis Methods 0.000 claims 1
- 229940088594 vitamin Drugs 0.000 claims 1
- 229930003231 vitamin Natural products 0.000 claims 1
- 235000013343 vitamin Nutrition 0.000 claims 1
- 239000011782 vitamin Substances 0.000 claims 1
- 238000012544 monitoring process Methods 0.000 abstract description 2
- 238000007669 thermal treatment Methods 0.000 abstract 1
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 7
- 238000002347 injection Methods 0.000 description 6
- 239000007924 injection Substances 0.000 description 6
- 239000000758 substrate Substances 0.000 description 6
- 238000005070 sampling Methods 0.000 description 5
- 239000007788 liquid Substances 0.000 description 4
- 239000002245 particle Substances 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 238000013019 agitation Methods 0.000 description 3
- 238000013461 design Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- 238000000605 extraction Methods 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 2
- 229910001220 stainless steel Inorganic materials 0.000 description 2
- 239000010935 stainless steel Substances 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- 239000004809 Teflon Substances 0.000 description 1
- 229920006362 Teflon® Polymers 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000003638 chemical reducing agent Substances 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 231100000225 lethality Toxicity 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 238000009928 pasteurization Methods 0.000 description 1
- 229910052697 platinum Inorganic materials 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 235000008001 rakum palm Nutrition 0.000 description 1
- 238000005057 refrigeration Methods 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Sustainable Development (AREA)
- Biomedical Technology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Thermal Sciences (AREA)
- Cell Biology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Food Preservation Except Freezing, Refrigeration, And Drying (AREA)
Abstract
El nuevo Termorresistómetro incorpora un serpentín de refrigeración (6) y realiza el control de la temperatura con un PID a través de un autómata (11) programable y monitorización con un sistema Scada. Todo ello nos permite, además de realizar tratamientos térmicos a temperatura constante, simular rampas de calentamiento y de enfriamiento complejas y tratamientos térmicos completos, tanto discontinuos como continuos y registrar la evolución de la temperatura alcanzada.The new thermometer resistometer incorporates a cooling coil (6) and performs temperature control with a PID through a programmable automaton (11) and monitoring with a Scada system. All this allows us, in addition to performing heat treatments at a constant temperature, simulating complex heating and cooling ramps and complete thermal treatments, both discontinuous and continuous and recording the evolution of the temperature reached.
Description
Termorresistómetro para la medida de la resistencia al calor de microorganismos en condiciones controladas de temperatura, capaz de simular condiciones de tratamiento isotérmico y no isotermo.Thermometer resistometer for measuring the heat resistance of microorganisms under controlled conditions of temperature, capable of simulating treatment conditions Isothermal and non-isothermal.
El Termorresistómetro que se propone, permite simular los tratamientos térmicos aplicados a los alimentos (tanto por lotes como en continuo) y obtener datos fiables sobre la letalidad alcanzada en estos tratamientos que permitan su optimización, lo cual es una de las mayores preocupaciones actuales de las empresas del sector alimentario.The proposed thermometer resistor allows simulate heat treatments applied to food (both in batches as in continuous) and obtain reliable data on the lethality achieved in these treatments that allow their optimization, which is one of the biggest concerns today of companies in the food sector.
La determinación de la resistencia al calor de los microorganismos resulta de vital importancia a la hora de establecer la intensidad de los tratamientos térmicos a aplicar a los alimentos apertizados.The determination of heat resistance of the microorganisms is of vital importance at the time of establish the intensity of the heat treatments to be applied to the opened foods.
Existen en la actualidad diversos métodos disponibles para determinar la resistencia microbiana al calor, que pueden ser agrupados en: indirectos, directos, de partícula y de mezcla, pero en ninguno de ellos se consigue simulación de rampas de calentamiento y de enfriamiento complejas ni tratamientos térmicos completos, tanto discontinuos como continuos.There are currently several methods available to determine microbial heat resistance, which They can be grouped into: indirect, direct, particle and mix, but in none of them you get ramp simulation complex heating and cooling or treatments full thermal, both discontinuous and continuous.
En los métodos indirectos, la suspensión microbiana, introducida en finos capilares o en tubos de vidrio, se calienta al ser éstos introducidos en un baño termostatado a la temperatura de tratamiento. El principal inconveniente de estos métodos es el retraso inherente en el calentamiento y enfriamiento de las muestras, lo que impide su uso a temperaturas elevadas, en el rango UHT.In indirect methods , the microbial suspension, introduced into thin capillaries or glass tubes, is heated as they are introduced into a thermostated bath at the treatment temperature. The main drawback of these methods is the inherent delay in heating and cooling the samples, which prevents their use at elevated temperatures, in the UHT range.
El método de los capilares fue diseñado por Stern y Proctor en 1954 y ha sido denominado frecuentemente como método de referencia. Con el uso de capilares de paredes finas (0,15 mm) se reducen considerablemente los tiempos requeridos para el calentamiento y el enfriamiento o fases de inercia. Sin embargo, el cierre, la apertura y el vaciado de tubos es una tarea laboriosa, especialmente cuando se trabaja con alimentos viscosos.The capillary method was designed by Stern and Proctor in 1954 and has been frequently referred to as a method reference. With the use of thin-walled capillaries (0.15 mm) the time required for the heating and cooling or phases of inertia. However the closing, opening and emptying tubes is a laborious task, especially when working with viscous foods.
Los métodos directos son complejos, no permiten trabajar a temperaturas bajas (por debajo de 100ºC) y, en la mayoría de ellos, no se puede trabajar con alimentos. Se trata de los denominados termorresistómetros, de los cuales existen distintos tipos, como son: Direct methods are complex, they do not allow working at low temperatures (below 100ºC) and, in most of them, you cannot work with food. These are called thermoresistometers, of which there are different types, such as:
El termorresistómetro de Stumbo fue diseñado para estudiar la termorresistencia a temperaturas comprendidas entre 104 y 150ºC (Stumbo, 1948), donde los recuentos de los endosporos viables se determinan por la técnica del número más probable.The Stumbo thermometer resistor was designed to study the thermometer resistance at temperatures between 104 and 150 ° C (Stumbo, 1948), where viable endosporus counts are determined by the most probable number technique.
El termorresistómetro de Pflug y Esselen (1953) está basado en el termorresistómetro de Stumbo, con una serie de modificaciones, que permiten controlar la temperatura con un error menor.The Pflug and Esselen thermometer resistor (1953) is based on the Stumbo thermometer resistor, with a series of modifications, which allow the temperature to be controlled with a minor error.
El termorresistómetro de David y Merson (1990) fue especialmente desarrollado para estudiar los parámetros de termorresistencia a temperaturas de hasta 155ºC.The thermometer resistor of David and Merson (1990) was specially developed to study the thermometer resistance parameters at temperatures up to 155ºC.
Basándose en las ideas anteriores, Brown et al. (1988) desarrollaron un termorresistómetro que incorpora unas notables mejoras respecto a los diseños anteriores. En el denominado termorresistómetro de Campdem, el depósito o tanque de vapor es al mismo tiempo la cámara de tratamiento, lo cual es una gran ventaja para mantener estable la temperatura durante el tratamiento térmico. Es una tubería colocada en posición vertical, lo que logra un ahorro de espacio en el laboratorio y mejora el control de la temperatura. Al igual que el modelo anterior, la carga y descarga se realiza por la antecámara, que también está refrigerada por agua y posee un sistema de inyección de aire a presión. La muestra se coloca en un disco de papel, al igual que lo hacen David y Merson (1990), pero sin situarla entre discos hidrófobos. De esta forma el vapor entra en contacto con las dos caras del disco de papel y favorece un calentamiento casi instantáneo. Un termopar tipo T obtiene un registro térmico de la muestra. Para controlar la temperatura de la cámara se emplean cuatro termómetros de resistencia de platino, que se sitúan muy próximos a las muestras. Las señales que envían son procesadas por un ordenador que ajusta la temperatura mediante la apertura o cierre de unas electroválvulas conectadas a la caldera de vapor. Todo el control y ejecución de los tratamientos está dirigido por un ordenador.Based on the above ideas, Brown et al . (1988) developed a thermometer resistor that incorporates some notable improvements over previous designs. In the so-called Campdem thermoresistometer , the steam tank or tank is at the same time the treatment chamber, which is a great advantage to keep the temperature stable during the heat treatment. It is a pipe placed in an upright position, which saves space in the laboratory and improves temperature control. Like the previous model, the loading and unloading is done by the antechamber, which is also water cooled and has a pressurized air injection system. The sample is placed on a paper disk, just like David and Merson (1990), but without placing it between hydrophobic discs. In this way the steam comes into contact with both sides of the paper disk and favors almost instantaneous heating. A type T thermocouple obtains a thermal record of the sample. To control the temperature of the chamber, four platinum resistance thermometers are used, which are located very close to the samples. The signals they send are processed by a computer that adjusts the temperature by opening or closing solenoid valves connected to the steam boiler. All control and execution of treatments is managed by a computer.
Los métodos de partícula son los denominados integradores tiempo-temperatura (ITTs). Se trata de partículas con compuestos (substancias químicas o microorganismos) de termorresistencia conocida que se introducen en los alimentos para evaluar la intensidad del tratamiento térmico a que son sometidos. Más que métodos de determinación de termorresistencia se han empleado como sistemas para validar los tratamientos térmicos. Particle methods are called time-temperature integrators (ITTs). These are particles with compounds (chemical substances or microorganisms) of known heat resistance that are introduced into food to assess the intensity of the heat treatment to which they are subjected. More than heat resistance determination methods, they have been used as systems to validate heat treatments.
Los métodos de mezcla pretenden reducir la fase de inercia de calentamiento de la suspensión de microorganismos al mezclar pequeños volúmenes de la misma con volúmenes mucho mayores de substratos precalentados.The mixing methods are intended to reduce the heating inertia phase of the microorganism suspension by mixing small volumes thereof with much larger volumes of preheated substrates.
Existen distintos métodos de mezcla tales como el método del matraz, que únicamente se emplea para estudiar la termorresistencia de bacterias a temperaturas inferiores al punto de ebullición del agua, generalmente organismos no esporulados o esporulados de baja termorresistencia.There are different mixing methods such as the flask method , which is only used to study the heat resistance of bacteria at temperatures below the boiling point of water, generally non-sporulated or sporulated organisms of low heat resistance.
El método de Kooiman y Geers (1975) es un sistema eficaz para establecer la resistencia térmica de microorganismos y que no requiere de unas instalaciones costosas. No obstante, la limitación deriva del tiempo de inercia durante el enfriamiento. La siembra y recuento de supervivientes es laborioso.The method of Kooiman and Geers (1975) is an effective system to establish the thermal resistance of microorganisms and does not require expensive facilities. However, the limitation derives from the inertia time during cooling. Sowing and counting survivors is laborious.
El termorresistómetro TR-SC fue puesto en funcionamiento por el equipo de los Dres. Sala y Condón, en la Universidad de Zaragoza. Su funcionamiento consiste en precalentar, con una resistencia eléctrica (5) dispuesta en un pequeño tanque, el substrato donde se va a determinar la termorresistencia. El calentamiento de los microorganismos es instantáneo, al entrar éstos en contacto con el substrato precalentado a la temperatura de tratamiento, y los substratos pueden ser líquidos fluidos o viscosos, o líquidos con partículas. Su diseño permite trabajar desde temperaturas de pasteurización hasta temperaturas de la gama de UHT (135.8º), así como esterilizar en su interior el substrato a emplear. También proporciona un seguimiento constante de la evolución del pH del substrato.The TR-SC thermometer resistor was put into operation by Drs. Sala y Condón, at the University of Zaragoza. Its operation consists in preheating, with an electrical resistance (5) arranged in a small tank, the substrate where the heat resistance is to be determined. The heating of the microorganisms is instantaneous, when they come into contact with the preheated substrate at the treatment temperature, and the substrates can be fluid or viscous liquids, or liquids with particles. Its design allows to work from pasteurization temperatures to temperatures of the UHT range (135.8º), as well as sterilize the substrate to be used inside. It also provides constant monitoring of the evolution of the substrate pH.
El instrumento que deseamos patentar es un equipo diseñado para simular tratamientos térmicos que se aplican habitualmente en la industria alimentaria. Su diseño permite trabajar con medios de calentamiento líquidos tales como tampones o alimentos líquidos o finamente particulados e inocular microorganismos o compuestos diversos y obtener muestras para estudiar la evolución de esos microorganismos o compuestos a lo largo del tratamiento térmico.The instrument we want to patent is a team designed to simulate heat treatments that are applied usually in the food industry. Its design allows work with liquid heating media such as buffers or liquid or finely particulate and inocular foods various microorganisms or compounds and obtain samples for study the evolution of these microorganisms or compounds at Long heat treatment.
El termorresistómetro consta de un vaso principal en el que se aplican los tratamientos térmicos, un motor para garantizar la homogeneidad del medio de calentamiento, una unidad principal de control, mediante la que se regulan el calentamiento, la toma de muestras y la agitación, una fuente externa de presión y un colector de fracciones, para permitir tomar muestras en experimentos de corta duración.The thermometer resistor consists of a main vessel in which the heat treatments are applied, an engine for guarantee the homogeneity of the heating medium, one unit main control, by which the heating is regulated, sampling and agitation, an external source of pressure and a fraction collector, to allow samples to be taken in Short duration experiments.
Fig. 1 - Corresponde al vaso principal, unido por una unidad principal de control que conecta a un ordenador mediante software SCADA.Fig. 1 - Corresponds to the main vessel, joined by a main control unit that connects to a computer through SCADA software.
En dichas figuras, las referencias numéricas corresponden a las siguientes partes y elementos:In these figures, the numerical references correspond to the following parts and elements:
- 1.one.
- Vaso principal.Glass principal.
- 2.2.
- Eje de agitación.Axis of agitation
- 3.3.
- HélicePropeller
- 4.Four.
- MotorEngine
- 5.5.
- Resistencia eléctricaElectric resistance
- 6.6.
- Serpentín de refrigeraciónCooling coil
- 7.7.
- SondaProbe
- 8.8.
- Jeringuilla de inyección de microorganismosInjection syringe microorganisms
- 9.9.
- Tubo de muestreoTube Of sampling
- 10.10.
- Nitrógeno seco.Dry nitrogen
- 11.eleven.
- Unidad principal de control o autómataMain control unit or automaton
- 12.12.
- Válvula solenoide de pinzamientoSolenoid valve grip
- 13.13.
- OrdenadorComputer
- 14.14.
- Puerto de inyecciónInjection port
- 15.fifteen.
- ManoreductorManoreductor
- 16.16.
- Válvula del serpentín de refrigeración (6)Cooling coil valve (6)
- 17.17.
- Agua de refrigeraciónWater of refrigeration
El vaso principal (1) es de acero inoxidable de 400 ml (medidas externas: 8,5 cm de diámetro x 12 cm de altura) con una tapa roscada, cerrada mediante una junta tórica de estanqueidad.The main vessel (1) is made of stainless steel 400 ml (external measurements: 8.5 cm in diameter x 12 cm in height) with a threaded cover, closed by an O-ring tightness
La tapa alberga un tubo que sirve de guía para el eje de agitación (2), el cual está dotado de una hélice (3) en su extremo inferior y es accionado por el motor (4). La tapa además alberga otros 7 puertos: dos de ellos son para los brazos de la resistencia eléctrica (5), dos para los brazos del serpentín de refrigeración (6), con una válvula (16), uno para una sonda Pt-100 (7) que determina la temperatura durante el tratamiento, uno para la inyección de microorganismos y un último para el tubo de muestreo (9). Finalmente la tapa está dotada de un conector rápido de presión a través del cual se conecta el instrumento a la fuente de presión externa (botella de nitrógeno seco) (10).The lid houses a tube that serves as a guide for the stirring shaft (2), which is provided with a propeller (3) in its lower end and is driven by the motor (4). The lid also it houses another 7 ports: two of them are for the arms of the electrical resistance (5), two for the coil arms of cooling (6), with a valve (16), one for a probe Pt-100 (7) that determines the temperature during treatment, one for the injection of microorganisms and one last for the sampling tube (9). Finally the lid is equipped with a quick pressure connector through which the instrument to the external pressure source (nitrogen bottle dry) (10).
La agitación del contenido del termorresistómetro se consigue mediante un motor (4) que acciona al eje con la hélice (3), a través de un alambre acerado. La velocidad del motor (4) es regulada por un variador de frecuencia a través del autómata (11). La homogeneidad se ve favorecida por la presencia en el interior del instrumento de una pantalla deflectora que mejora la turbulencia.Agitation of the contents of the thermometer is achieved by a motor (4) that drives the shaft with the propeller (3), through a steel wire. The engine speed (4) is regulated by a frequency inverter through the automaton (11). Homogeneity is favored by the presence inside of the instrument of a deflector screen that improves the turbulence.
El tubo de muestreo (9) es de acero inoxidable, disponiéndose de tubos intercambiables de distintos diámetros internos, desde 0,5 hasta 2 mm, y es prolongado en su extremo final por un tubo de silicona. Este último es cerrado por una válvula solenoide de pinzamiento (12), cuya apertura puede ser accionada a través de un interruptor en la unidad principal de control ó autómata (11). La electroválvula, normalmente cerrada, mantiene cerrado el tubo de extracción, incluso cuando el vaso principal (1) está presurizado, y únicamente se abre cuando es accionada desde la unidad principal, para tomar muestras. El tiempo de apertura de la electroválvula puede ser regulado mediante un temporizador a través del autómata (11).The sampling tube (9) is made of stainless steel, having interchangeable tubes of different diameters internal, from 0.5 to 2 mm, and is prolonged at its end For a silicone tube. The latter is closed by a valve clamp solenoid (12), whose opening can be operated at through a switch in the main control unit or automaton (11). The solenoid valve, normally closed, keeps closed the extraction tube, even when the main vessel (1) It is pressurized, and only opens when operated from the main unit, to take samples. The opening time of the solenoid valve can be regulated by a timer through of the automaton (11).
El autómata (11) permite también el control de la temperatura. Para ello dispone de un PID conectado a la resistencia eléctrica (5) de 1100 w, a una válvula que regula el flujo de agua de refrigeración (17) a través del serpentín de refrigeración (6), y a la sonda Pt-100 (7). El PID, así como la válvula de muestreo y el motor (4) de agitación son controlados mediante un autómata (11) programable que permite realizar rampas de calentamiento y perfiles de temperatura complejos. El autómata (11) se puede conectar a un ordenador y, mediante un software específico Scada, permite el registro de los datos de temperatura y tiempo de tratamiento de la muestra. Este software también permite la programación de los perfiles de temperatura a realizar, directamente a través del ordenador.The automaton (11) also allows the control of the temperature. For this it has a PID connected to the resistor electrical (5) of 1100 w, to a valve that regulates the flow of water cooling (17) through the cooling coil (6), and to the Pt-100 probe (7). The PID, as well as the sampling valve and stirring motor (4) are controlled by means of a programmable automaton (11) that allows ramps of heating and complex temperature profiles. The automaton (11) can be connected to a computer and, through software specific Scada, allows the recording of temperature data and Sample treatment time. This software also allows the programming of the temperature profiles to be carried out, directly through the computer.
El instrumento se puede mantener bajo presión gracias a la existencia de juntas de teflón (PTFE) en todos los puertos, incluido el del eje. El puerto de inyección (14) de microorganismos (8) se cierra mediante un septo de cromatografía. La presión externa la proporciona una botella de nitrógeno seco (10) y se regula mediante un manoreductor (15). Esta presión permite la extracción de muestras a bajas temperaturas de trabajo (inferiores a 100ºC) o cuando el medio de calentamiento es demasiado viscoso. El mantenimiento de una presión constante de trabajo a lo largo de un experimento y de un tiempo de apertura de la válvula solenoide (12) también constante, permiten obtener muestras de idéntico volumen a lo largo del experimento. La inyección de muestras se puede realizar mediante una jeringuilla (8) médica estéril o bien mediante una tipo Hamilton, cuando el instrumento se haya bajo presión.The instrument can be kept under pressure thanks to the existence of teflon gaskets (PTFE) in all ports, including the axis. The injection port (14) of microorganisms (8) is closed by a chromatographic septum. The external pressure is provided by a bottle of dry nitrogen (10) and is regulated by means of a hand reducer (15). This pressure allows the extraction of samples at low working temperatures (below 100 ° C) or when the heating medium is too viscous Maintaining a constant pressure of I work throughout an experiment and an opening time of the solenoid valve (12) also constant, allow to obtain samples of identical volume throughout the experiment. The Sample injection can be performed using a syringe (8) sterile medical or by a Hamilton type, when the instrument is under pressure.
Claims (7)
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES200302529A ES2231030B1 (en) | 2003-10-29 | 2003-10-29 | THERMRESISTOMETER FOR MEASURING THE HEAT RESISTANCE OF MICROORGANISMS IN TEMPERATURE CONTROLLED CONDITIONS, ABLE TO SIMULATE ISOTHERMAL AND NON-ISOTHERMAL TREATMENT CONDITIONS. |
| PCT/ES2004/000472 WO2005039323A1 (en) | 2003-10-29 | 2004-10-28 | Thermoresistometer for measuring the resistance to heat of micro-organisms in temperature controlled conditions and for simulating isothermal and non-isothermal treatment conditions |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES200302529A ES2231030B1 (en) | 2003-10-29 | 2003-10-29 | THERMRESISTOMETER FOR MEASURING THE HEAT RESISTANCE OF MICROORGANISMS IN TEMPERATURE CONTROLLED CONDITIONS, ABLE TO SIMULATE ISOTHERMAL AND NON-ISOTHERMAL TREATMENT CONDITIONS. |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| ES2231030A1 ES2231030A1 (en) | 2005-05-01 |
| ES2231030B1 true ES2231030B1 (en) | 2006-07-16 |
Family
ID=34507901
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES200302529A Expired - Fee Related ES2231030B1 (en) | 2003-10-29 | 2003-10-29 | THERMRESISTOMETER FOR MEASURING THE HEAT RESISTANCE OF MICROORGANISMS IN TEMPERATURE CONTROLLED CONDITIONS, ABLE TO SIMULATE ISOTHERMAL AND NON-ISOTHERMAL TREATMENT CONDITIONS. |
Country Status (2)
| Country | Link |
|---|---|
| ES (1) | ES2231030B1 (en) |
| WO (1) | WO2005039323A1 (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2648968A1 (en) * | 2016-07-05 | 2018-01-09 | Inbiolev, S.L. | Biorreactor for the multiplication of yeasts and lactic bacteria (Machine-translation by Google Translate, not legally binding) |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4212950A (en) * | 1978-05-08 | 1980-07-15 | The Virtis Company, Inc. | Fermenting apparatus |
| US4424559A (en) * | 1981-02-27 | 1984-01-03 | New Brunswick Scientific Co., Inc. | Modular instrumentation for monitoring and control of biochemical processes |
| ES2004913A6 (en) * | 1987-04-02 | 1989-02-16 | Univ Zaragoza | Thermotresistometer for measuring microbial thermal resistance in foods in controlled ph and temperature conditions (Machine-translation by Google Translate, not legally binding) |
| ES2046944B1 (en) * | 1992-03-31 | 1994-08-16 | Univ Zaragoza | PROCEDURE FOR THE DESTRUCTION OF MICROORGANISMS AND ENZYMES THROUGH THE COMBINED APPLICATION OF HEAT AND ULTRASONICS UNDER PRESSURE: MTS PROCESS (HAND-THERMO-SONICATION). |
| US6906172B2 (en) * | 1998-03-10 | 2005-06-14 | Large Scale Biology Corporation | Flexible processing apparatus for isolating and purifying viruses, soluble proteins and peptides from plant sources |
| US20020156542A1 (en) * | 2001-02-23 | 2002-10-24 | Nandi Hill K. | Methods, devices and systems for monitoring, controlling and optimizing processes |
-
2003
- 2003-10-29 ES ES200302529A patent/ES2231030B1/en not_active Expired - Fee Related
-
2004
- 2004-10-28 WO PCT/ES2004/000472 patent/WO2005039323A1/en active Application Filing
Also Published As
| Publication number | Publication date |
|---|---|
| ES2231030A1 (en) | 2005-05-01 |
| WO2005039323A1 (en) | 2005-05-06 |
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