ES2199058B1 - Reactivo de latex para la deteccion de anticuerpos frente a micoplasmaneumoniae. - Google Patents
Reactivo de latex para la deteccion de anticuerpos frente a micoplasmaneumoniae.Info
- Publication number
- ES2199058B1 ES2199058B1 ES200201298A ES200201298A ES2199058B1 ES 2199058 B1 ES2199058 B1 ES 2199058B1 ES 200201298 A ES200201298 A ES 200201298A ES 200201298 A ES200201298 A ES 200201298A ES 2199058 B1 ES2199058 B1 ES 2199058B1
- Authority
- ES
- Spain
- Prior art keywords
- sensitized
- mycoplasma pneumoniae
- particles
- antibodies against
- detection
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56911—Bacteria
- G01N33/56933—Mycoplasma
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Reactivo de látex para la detección de anticuerpos frente a Mycoplasma pneumoniae. Se describe un procedimiento (test de inmunodiagnóstico) para detectar y cuantificar anticuerpos específicos de Mycoplasma pneumoniae presentes en muestras serológicas, mediante la aglutinación-sedimentación de partículas de látex sensibilizadas con un antígeno lipídico de Mycoplasma pneumoniae. Asimismo se describe el método de obtención de las partículas de látex sensibilizadas y el tampón de reacción donde se desarrolla la inmunorreacción. El desarrollo del inmunodiagnóstico tiene lugar en los pocillos de una placa de microtitulación mezclando las partículas sensibilizadas con muestras de sueros humanos en un tampón de reacción específico. La presencia en el suero de anticuerpos frente Mycoplasma pneumoniae se detecta mediante la aglutinación de las partículas de látex sensibilizadas. La ausencia de anticuerpos específicos se detecta mediante la sedimentación de éstas partículas. Este test de inmunodiagnóstico es económico, técnicamente simple, tiene una elevada estabilidad temporal y presenta unos niveles de sensibilidad y especificidad adecuados.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES200201298A ES2199058B1 (es) | 2002-06-06 | 2002-06-06 | Reactivo de latex para la deteccion de anticuerpos frente a micoplasmaneumoniae. |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES200201298A ES2199058B1 (es) | 2002-06-06 | 2002-06-06 | Reactivo de latex para la deteccion de anticuerpos frente a micoplasmaneumoniae. |
Publications (2)
Publication Number | Publication Date |
---|---|
ES2199058A1 ES2199058A1 (es) | 2004-02-01 |
ES2199058B1 true ES2199058B1 (es) | 2005-02-16 |
Family
ID=31198096
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES200201298A Expired - Fee Related ES2199058B1 (es) | 2002-06-06 | 2002-06-06 | Reactivo de latex para la deteccion de anticuerpos frente a micoplasmaneumoniae. |
Country Status (1)
Country | Link |
---|---|
ES (1) | ES2199058B1 (es) |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS566161A (en) * | 1979-06-28 | 1981-01-22 | Seikagaku Kogyo Co Ltd | Erythropoietin sensitized latex |
JPS60209179A (ja) * | 1984-04-02 | 1985-10-21 | Tetsuo Tomiyama | 肺炎マイコプラズマ脂質抗原レシチン複合物感作ラテツクス試薬及びそれを用いた肺炎マイコプラズマ抗体の検出方法 |
WO1992014154A1 (en) * | 1991-02-12 | 1992-08-20 | Dynagen, Inc. | Agglutination test for mycobacterial antigens in biological samples |
-
2002
- 2002-06-06 ES ES200201298A patent/ES2199058B1/es not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
ES2199058A1 (es) | 2004-02-01 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EC2A | Search report published |
Date of ref document: 20040201 Kind code of ref document: A1 |
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FD2A | Announcement of lapse in spain |
Effective date: 20220629 |