ES2195741B1 - Sistema de recombinacion in vitro para la expresion de productos viricos. - Google Patents

Sistema de recombinacion in vitro para la expresion de productos viricos.

Info

Publication number
ES2195741B1
ES2195741B1 ES200102047A ES200102047A ES2195741B1 ES 2195741 B1 ES2195741 B1 ES 2195741B1 ES 200102047 A ES200102047 A ES 200102047A ES 200102047 A ES200102047 A ES 200102047A ES 2195741 B1 ES2195741 B1 ES 2195741B1
Authority
ES
Spain
Prior art keywords
expression
gag
recombination system
vitro recombination
products
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES200102047A
Other languages
English (en)
Other versions
ES2195741A1 (es
Inventor
Antonio Rodriguez-Noriega Belaustegui
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
CLINMICRO S L
CLINMICRO SL
Original Assignee
CLINMICRO S L
CLINMICRO SL
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by CLINMICRO S L, CLINMICRO SL filed Critical CLINMICRO S L
Priority to ES200102047A priority Critical patent/ES2195741B1/es
Publication of ES2195741A1 publication Critical patent/ES2195741A1/es
Application granted granted Critical
Publication of ES2195741B1 publication Critical patent/ES2195741B1/es
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

Sistema de recombinación in vitro para la expresión de productos víricos, aplicado concretamente a la obtención de un fragmento representativo de la secuencia completa de los genes gag y pol del virus VIH del plasma de pacientes infectados, la construcción de un plásmido auxiliar con la totalidad de los genes gag y pol de un virus estándar, el procedimiento necesario para la realización de la reacción recombinante, la construcción de un módulo de expresión genética que contenga los genes gag y pol del virus del paciente, la producción de vectores retrovirales y el estudio de resistencias fenotípicas a antiretrovirales.
ES200102047A 2001-09-11 2001-09-11 Sistema de recombinacion in vitro para la expresion de productos viricos. Expired - Fee Related ES2195741B1 (es)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES200102047A ES2195741B1 (es) 2001-09-11 2001-09-11 Sistema de recombinacion in vitro para la expresion de productos viricos.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES200102047A ES2195741B1 (es) 2001-09-11 2001-09-11 Sistema de recombinacion in vitro para la expresion de productos viricos.

Publications (2)

Publication Number Publication Date
ES2195741A1 ES2195741A1 (es) 2003-12-01
ES2195741B1 true ES2195741B1 (es) 2005-04-01

Family

ID=29762958

Family Applications (1)

Application Number Title Priority Date Filing Date
ES200102047A Expired - Fee Related ES2195741B1 (es) 2001-09-11 2001-09-11 Sistema de recombinacion in vitro para la expresion de productos viricos.

Country Status (1)

Country Link
ES (1) ES2195741B1 (es)

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5837464A (en) * 1996-01-29 1998-11-17 Virologic, Inc. Compositions and methods for determining anti-viral drug susceptibility and resistance and anti-viral drug screening
US6103462A (en) * 1998-05-29 2000-08-15 Institut Pasteur Rapid single-cycle assay for human immunodeficiency virus type-1 drug resistance

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
PETROPOULOS, C.J.; PARKIN, N.T.; LIMOLI, K.L. et al. A novel phenotypic drug susceptibility assay for Human Immunodeficiency Virus Type 1. Antimicrobial Agents and Chemotherapy. Abril 2000, Vol. 44, Nº 4, páginas 920-928. *

Also Published As

Publication number Publication date
ES2195741A1 (es) 2003-12-01

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