ES2190866B1 - Metodo para la insercion eficiente en sitios especificos de cualquier adn diana de intrones del grupo ii. - Google Patents
Metodo para la insercion eficiente en sitios especificos de cualquier adn diana de intrones del grupo ii.Info
- Publication number
- ES2190866B1 ES2190866B1 ES200100170A ES200100170A ES2190866B1 ES 2190866 B1 ES2190866 B1 ES 2190866B1 ES 200100170 A ES200100170 A ES 200100170A ES 200100170 A ES200100170 A ES 200100170A ES 2190866 B1 ES2190866 B1 ES 2190866B1
- Authority
- ES
- Spain
- Prior art keywords
- dna
- group
- intron
- specific sites
- sequences
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Método para la inserción eficiente en sitios específicos de cualquier ADN diana de intrones del grupo II. El método permite la inserción de una molécula de ácido nucleico en sitios específicos de cualquier ADN diana. El método consiste en: la disposición del ARN de un intrón del grupo II que contenga dos secuencias nucleotídicas capaces de hibridar con dos secuencias contenidas en una de las hebras del ADN sustrato; la modificación de las secuencias del ARN del intrón, que hibridan con el ADN sustrato, de tal manera que reconozcan secuencias específicas en una de las hebras de un sustrato dado de ADN; y por último, aportar una proteína codificada por el intrón que en su forma silvestre carece del dominio endonucleasa. El intrón del grupo II rediseñado, puede emplearse en cualquier aplicación in vivo o in vitro, y en particular en mutagénesis.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES200100170A ES2190866B1 (es) | 2001-01-25 | 2001-01-25 | Metodo para la insercion eficiente en sitios especificos de cualquier adn diana de intrones del grupo ii. |
| PCT/ES2002/000030 WO2002059362A1 (es) | 2001-01-25 | 2002-01-24 | Metodo para la insercion eficiente en sitios especificos de cualquier adn diana de intrones del grupo ii. |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES200100170A ES2190866B1 (es) | 2001-01-25 | 2001-01-25 | Metodo para la insercion eficiente en sitios especificos de cualquier adn diana de intrones del grupo ii. |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| ES2190866A1 ES2190866A1 (es) | 2003-08-16 |
| ES2190866B1 true ES2190866B1 (es) | 2004-12-01 |
Family
ID=8496505
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES200100170A Expired - Fee Related ES2190866B1 (es) | 2001-01-25 | 2001-01-25 | Metodo para la insercion eficiente en sitios especificos de cualquier adn diana de intrones del grupo ii. |
Country Status (2)
| Country | Link |
|---|---|
| ES (1) | ES2190866B1 (es) |
| WO (1) | WO2002059362A1 (es) |
-
2001
- 2001-01-25 ES ES200100170A patent/ES2190866B1/es not_active Expired - Fee Related
-
2002
- 2002-01-24 WO PCT/ES2002/000030 patent/WO2002059362A1/es not_active Application Discontinuation
Non-Patent Citations (5)
Also Published As
| Publication number | Publication date |
|---|---|
| ES2190866A1 (es) | 2003-08-16 |
| WO2002059362A1 (es) | 2002-08-01 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EC2A | Search report published |
Date of ref document: 20030816 Kind code of ref document: A1 |
|
| FG2A | Definitive protection |
Ref document number: 2190866B1 Country of ref document: ES |
|
| FD2A | Announcement of lapse in spain |
Effective date: 20180807 |