ES2188551T3 - Metodo para aumentar la velocidad de hibridacion de acidos nucleicos. - Google Patents

Metodo para aumentar la velocidad de hibridacion de acidos nucleicos.

Info

Publication number
ES2188551T3
ES2188551T3 ES00937982T ES00937982T ES2188551T3 ES 2188551 T3 ES2188551 T3 ES 2188551T3 ES 00937982 T ES00937982 T ES 00937982T ES 00937982 T ES00937982 T ES 00937982T ES 2188551 T3 ES2188551 T3 ES 2188551T3
Authority
ES
Spain
Prior art keywords
nucleic acid
nucleic acids
hybridization
speed
increase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES00937982T
Other languages
English (en)
Inventor
Jaime E Ramirez-Vick
Simon Chin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Iris Biotechnologies Inc
Original Assignee
Iris Biotechnologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Iris Biotechnologies Inc filed Critical Iris Biotechnologies Inc
Application granted granted Critical
Publication of ES2188551T3 publication Critical patent/ES2188551T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Un método para aumentar la velocidad de hibridación de ácidos nucleicos en un ensayo de ácidos nucleicos, comprendiendo dicho método: a) unir moléculas de sonda de ácidos nucleicos de secuencia conocida a un soporte sólido; b) marcar moléculas de ácidos nucleicos diana con marcadores paramagnéticos; c) poner en contacto las moléculas diana marcadas con el soporte sólido; d) activar un campo magnético por medio del cual las moléculas marcadas son atraídas hacia el soporte sólido; e) lavar el soporte e invertir la polaridad del campo magnético para eliminar cualesquiera moléculas no unidas o unidas de forma no específica; y f) detectar las moléculas de ácidos nucleicos diana hibridadas.
ES00937982T 1999-05-27 2000-05-30 Metodo para aumentar la velocidad de hibridacion de acidos nucleicos. Expired - Lifetime ES2188551T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US13673799P 1999-05-27 1999-05-27

Publications (1)

Publication Number Publication Date
ES2188551T3 true ES2188551T3 (es) 2003-07-01

Family

ID=22474143

Family Applications (1)

Application Number Title Priority Date Filing Date
ES00937982T Expired - Lifetime ES2188551T3 (es) 1999-05-27 2000-05-30 Metodo para aumentar la velocidad de hibridacion de acidos nucleicos.

Country Status (9)

Country Link
EP (1) EP1206572B1 (es)
AT (1) ATE232560T1 (es)
AU (1) AU5308600A (es)
CA (1) CA2409523C (es)
DE (1) DE60001425T2 (es)
DK (1) DK1206572T3 (es)
ES (1) ES2188551T3 (es)
HK (1) HK1047301B (es)
WO (1) WO2000073506A1 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109652498A (zh) * 2012-05-30 2019-04-19 李忠 生物标记物的瞬时检测及其用途

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ZA877772B (en) * 1986-10-23 1988-04-20 Amoco Corporation Target and background capture methods and apparatus for affinity assays
US5039606A (en) * 1987-10-29 1991-08-13 Virginia Mason Research Center Diagnostic probe for diabetes type I predisposition
EP0503000A4 (en) * 1989-12-01 1993-04-14 The Board Of Trustees Of The Leland Stanford Junior University Promotion of high specificity molecular assembly
US5605662A (en) * 1993-11-01 1997-02-25 Nanogen, Inc. Active programmable electronic devices for molecular biological analysis and diagnostics

Also Published As

Publication number Publication date
AU5308600A (en) 2000-12-18
CA2409523C (en) 2008-12-30
ATE232560T1 (de) 2003-02-15
WO2000073506A1 (en) 2000-12-07
HK1047301B (zh) 2003-05-23
HK1047301A1 (en) 2003-02-14
DK1206572T3 (da) 2003-05-26
EP1206572B1 (en) 2003-02-12
DE60001425T2 (de) 2003-11-20
DE60001425D1 (de) 2003-03-20
CA2409523A1 (en) 2000-12-07
EP1206572A1 (en) 2002-05-22

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