ES2188551T3 - Metodo para aumentar la velocidad de hibridacion de acidos nucleicos. - Google Patents
Metodo para aumentar la velocidad de hibridacion de acidos nucleicos.Info
- Publication number
- ES2188551T3 ES2188551T3 ES00937982T ES00937982T ES2188551T3 ES 2188551 T3 ES2188551 T3 ES 2188551T3 ES 00937982 T ES00937982 T ES 00937982T ES 00937982 T ES00937982 T ES 00937982T ES 2188551 T3 ES2188551 T3 ES 2188551T3
- Authority
- ES
- Spain
- Prior art keywords
- nucleic acid
- nucleic acids
- hybridization
- speed
- increase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6832—Enhancement of hybridisation reaction
Abstract
Un método para aumentar la velocidad de hibridación de ácidos nucleicos en un ensayo de ácidos nucleicos, comprendiendo dicho método: a) unir moléculas de sonda de ácidos nucleicos de secuencia conocida a un soporte sólido; b) marcar moléculas de ácidos nucleicos diana con marcadores paramagnéticos; c) poner en contacto las moléculas diana marcadas con el soporte sólido; d) activar un campo magnético por medio del cual las moléculas marcadas son atraídas hacia el soporte sólido; e) lavar el soporte e invertir la polaridad del campo magnético para eliminar cualesquiera moléculas no unidas o unidas de forma no específica; y f) detectar las moléculas de ácidos nucleicos diana hibridadas.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US13673799P | 1999-05-27 | 1999-05-27 |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2188551T3 true ES2188551T3 (es) | 2003-07-01 |
Family
ID=22474143
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES00937982T Expired - Lifetime ES2188551T3 (es) | 1999-05-27 | 2000-05-30 | Metodo para aumentar la velocidad de hibridacion de acidos nucleicos. |
Country Status (9)
Country | Link |
---|---|
EP (1) | EP1206572B1 (es) |
AT (1) | ATE232560T1 (es) |
AU (1) | AU5308600A (es) |
CA (1) | CA2409523C (es) |
DE (1) | DE60001425T2 (es) |
DK (1) | DK1206572T3 (es) |
ES (1) | ES2188551T3 (es) |
HK (1) | HK1047301B (es) |
WO (1) | WO2000073506A1 (es) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108776227B (zh) * | 2012-05-30 | 2019-10-15 | 李忠 | 生物标记物的瞬时检测及其用途 |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ZA877772B (en) * | 1986-10-23 | 1988-04-20 | Amoco Corporation | Target and background capture methods and apparatus for affinity assays |
US5039606A (en) * | 1987-10-29 | 1991-08-13 | Virginia Mason Research Center | Diagnostic probe for diabetes type I predisposition |
US5474911A (en) * | 1989-12-01 | 1995-12-12 | The Board Of Trustees Of Leland Stanford Jr. University | Promotion of high specificity molecular assembly |
US5605662A (en) * | 1993-11-01 | 1997-02-25 | Nanogen, Inc. | Active programmable electronic devices for molecular biological analysis and diagnostics |
-
2000
- 2000-05-30 AU AU53086/00A patent/AU5308600A/en not_active Abandoned
- 2000-05-30 ES ES00937982T patent/ES2188551T3/es not_active Expired - Lifetime
- 2000-05-30 AT AT00937982T patent/ATE232560T1/de not_active IP Right Cessation
- 2000-05-30 CA CA002409523A patent/CA2409523C/en not_active Expired - Fee Related
- 2000-05-30 EP EP00937982A patent/EP1206572B1/en not_active Expired - Lifetime
- 2000-05-30 WO PCT/US2000/014969 patent/WO2000073506A1/en active IP Right Grant
- 2000-05-30 DE DE60001425T patent/DE60001425T2/de not_active Expired - Lifetime
- 2000-05-30 DK DK00937982T patent/DK1206572T3/da active
-
2002
- 2002-11-01 HK HK02107956.5A patent/HK1047301B/zh not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
ATE232560T1 (de) | 2003-02-15 |
EP1206572A1 (en) | 2002-05-22 |
HK1047301A1 (en) | 2003-02-14 |
DE60001425T2 (de) | 2003-11-20 |
CA2409523C (en) | 2008-12-30 |
HK1047301B (zh) | 2003-05-23 |
CA2409523A1 (en) | 2000-12-07 |
EP1206572B1 (en) | 2003-02-12 |
AU5308600A (en) | 2000-12-18 |
DE60001425D1 (de) | 2003-03-20 |
WO2000073506A1 (en) | 2000-12-07 |
DK1206572T3 (da) | 2003-05-26 |
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