ES2187964T3 - Procedimiento para la deteccion de alelos mutantes. - Google Patents
Procedimiento para la deteccion de alelos mutantes.Info
- Publication number
- ES2187964T3 ES2187964T3 ES98919054T ES98919054T ES2187964T3 ES 2187964 T3 ES2187964 T3 ES 2187964T3 ES 98919054 T ES98919054 T ES 98919054T ES 98919054 T ES98919054 T ES 98919054T ES 2187964 T3 ES2187964 T3 ES 2187964T3
- Authority
- ES
- Spain
- Prior art keywords
- alelos
- mutant
- procedure
- detection
- allels
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44747—Composition of gel or of carrier mixture
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/38—Flow patterns
- G01N30/46—Flow patterns using more than one column
- G01N30/466—Flow patterns using more than one column with separation columns in parallel
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Physics & Mathematics (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Dispersion Chemistry (AREA)
- Biophysics (AREA)
- Electrochemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Testing, Inspecting, Measuring Of Stereoscopic Televisions And Televisions (AREA)
- Analysing Materials By The Use Of Radiation (AREA)
Abstract
La invención se refiere a un procedimiento para la detección de alelos mutados en un exceso de alelos de tipo natural. Según el citado procedimiento, los citados alelos de tipo natural se aíslan mediante un procedimiento de separación que utiliza un vehículo que se une a uno o varios oligonucleótidos, siendo los citados oligonucleótidos complementarios con el alelo de tipo natural.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE19708758A DE19708758A1 (de) | 1997-03-04 | 1997-03-04 | Verfahren zum Nachweis von mutierten Allelen |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2187964T3 true ES2187964T3 (es) | 2003-06-16 |
Family
ID=7822190
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES98919054T Expired - Lifetime ES2187964T3 (es) | 1997-03-04 | 1998-03-04 | Procedimiento para la deteccion de alelos mutantes. |
Country Status (9)
Country | Link |
---|---|
EP (1) | EP0970249B1 (es) |
JP (1) | JP2001514504A (es) |
AT (1) | ATE228573T1 (es) |
AU (1) | AU7204298A (es) |
CA (1) | CA2283188A1 (es) |
DE (2) | DE19708758A1 (es) |
DK (1) | DK0970249T3 (es) |
ES (1) | ES2187964T3 (es) |
WO (1) | WO1998039472A2 (es) |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2897959B2 (ja) * | 1988-05-20 | 1999-05-31 | エフ.ホフマン―ラ ロシュ アクチェンゲゼルシャフト | 固定化された配列特異的プローブ |
US5352578A (en) * | 1989-02-15 | 1994-10-04 | Worcester Foundation For Experimental Biology | Method of separating oligonucleotides from a mixture |
JPH0430800A (ja) * | 1990-05-28 | 1992-02-03 | Tosoh Corp | 突然変異の検出方法 |
EP0461496B1 (en) * | 1990-06-08 | 1996-09-04 | BEHRINGWERKE Aktiengesellschaft | Method for the quantitative determination of DNA sequences |
WO1993022457A1 (en) * | 1992-04-24 | 1993-11-11 | Massachusetts Institute Of Technology | Screening for genetic variation |
US6027877A (en) * | 1993-11-04 | 2000-02-22 | Gene Check, Inc. | Use of immobilized mismatch binding protein for detection of mutations and polymorphisms, purification of amplified DNA samples and allele identification |
US5512441A (en) * | 1994-11-15 | 1996-04-30 | American Health Foundation | Quantative method for early detection of mutant alleles and diagnostic kits for carrying out the method |
-
1997
- 1997-03-04 DE DE19708758A patent/DE19708758A1/de not_active Ceased
-
1998
- 1998-03-04 AT AT98919054T patent/ATE228573T1/de not_active IP Right Cessation
- 1998-03-04 AU AU72042/98A patent/AU7204298A/en not_active Abandoned
- 1998-03-04 JP JP53807198A patent/JP2001514504A/ja not_active Ceased
- 1998-03-04 WO PCT/DE1998/000676 patent/WO1998039472A2/de active IP Right Grant
- 1998-03-04 DK DK98919054T patent/DK0970249T3/da active
- 1998-03-04 EP EP98919054A patent/EP0970249B1/de not_active Expired - Lifetime
- 1998-03-04 CA CA002283188A patent/CA2283188A1/en not_active Abandoned
- 1998-03-04 ES ES98919054T patent/ES2187964T3/es not_active Expired - Lifetime
- 1998-03-04 DE DE59806443T patent/DE59806443D1/de not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
ATE228573T1 (de) | 2002-12-15 |
EP0970249B1 (de) | 2002-11-27 |
DK0970249T3 (da) | 2003-03-17 |
WO1998039472A3 (de) | 1998-12-10 |
WO1998039472A2 (de) | 1998-09-11 |
DE59806443D1 (de) | 2003-01-09 |
AU7204298A (en) | 1998-09-22 |
DE19708758A1 (de) | 1998-09-17 |
CA2283188A1 (en) | 1998-09-11 |
JP2001514504A (ja) | 2001-09-11 |
EP0970249A2 (de) | 2000-01-12 |
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