ES2178576B1 - Procedimiento para la construccion de sistemas de expresion de proteinas en la rizosfera de plantas. - Google Patents
Procedimiento para la construccion de sistemas de expresion de proteinas en la rizosfera de plantas.Info
- Publication number
- ES2178576B1 ES2178576B1 ES200002799A ES200002799A ES2178576B1 ES 2178576 B1 ES2178576 B1 ES 2178576B1 ES 200002799 A ES200002799 A ES 200002799A ES 200002799 A ES200002799 A ES 200002799A ES 2178576 B1 ES2178576 B1 ES 2178576B1
- Authority
- ES
- Spain
- Prior art keywords
- construction
- expression systems
- plants
- procedure
- rhizosphere
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 108090000623 proteins and genes Proteins 0.000 title abstract 6
- 238000010276 construction Methods 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 4
- 102000004169 proteins and genes Human genes 0.000 title abstract 4
- 241000589776 Pseudomonas putida Species 0.000 abstract 2
- 102000003929 Transaminases Human genes 0.000 abstract 1
- 108090000340 Transaminases Proteins 0.000 abstract 1
- 241000607479 Yersinia pestis Species 0.000 abstract 1
- 238000006065 biodegradation reaction Methods 0.000 abstract 1
- 239000000356 contaminant Substances 0.000 abstract 1
- 101150021834 davT gene Proteins 0.000 abstract 1
- 210000000416 exudates and transudate Anatomy 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/78—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Pseudomonas
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1096—Transferases (2.) transferring nitrogenous groups (2.6)
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Cell Biology (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Virology (AREA)
- Dentistry (AREA)
- Environmental Sciences (AREA)
- Medicinal Chemistry (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Catching Or Destruction (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Procedimiento para la construcción de sistemas de expresión de proteínas en la rizosfera de plantas. La presente invención se refiere a un procedimiento para la construcción de sistemas de expresión eficaces en la rizosfera de plantas de proteínas de interés en agricultura, control biológico de plagas, o procesos de biodegradación de contaminantes. Para ello, se utiliza un gen de Pseudomonas putida cuyo promotor tiene probada funcionalidad en respuesta a exudados de raíces y semillas, para la construcción de sistemas de expresión en rizosfera. El gen es el davT de Pseudomonas putida, el cual codifica para la proteína d- aminovalerato aminotransferasa.
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES200002799A ES2178576B1 (es) | 2000-11-22 | 2000-11-22 | Procedimiento para la construccion de sistemas de expresion de proteinas en la rizosfera de plantas. |
AU2002220754A AU2002220754A1 (en) | 2000-11-22 | 2001-11-21 | System for expressing proteins in the rhizosphere of plants and method for building said system |
PCT/ES2001/000446 WO2002042476A1 (es) | 2000-11-22 | 2001-11-21 | Sistema de expresion de proteinas en la rizosfera de plantas y procedimiento para su construccion |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES200002799A ES2178576B1 (es) | 2000-11-22 | 2000-11-22 | Procedimiento para la construccion de sistemas de expresion de proteinas en la rizosfera de plantas. |
Publications (2)
Publication Number | Publication Date |
---|---|
ES2178576A1 ES2178576A1 (es) | 2002-12-16 |
ES2178576B1 true ES2178576B1 (es) | 2004-08-16 |
Family
ID=8495724
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES200002799A Expired - Fee Related ES2178576B1 (es) | 2000-11-22 | 2000-11-22 | Procedimiento para la construccion de sistemas de expresion de proteinas en la rizosfera de plantas. |
Country Status (3)
Country | Link |
---|---|
AU (1) | AU2002220754A1 (es) |
ES (1) | ES2178576B1 (es) |
WO (1) | WO2002042476A1 (es) |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5397697A (en) * | 1989-01-10 | 1995-03-14 | Ciba-Geigy Corporation | Identification of plant-responsive genes of bacteria |
JPH07509605A (ja) * | 1992-07-02 | 1995-10-26 | ノバルティス アクチェンゲゼルシャフト | 遺伝子活性化要素 |
-
2000
- 2000-11-22 ES ES200002799A patent/ES2178576B1/es not_active Expired - Fee Related
-
2001
- 2001-11-21 WO PCT/ES2001/000446 patent/WO2002042476A1/es not_active Application Discontinuation
- 2001-11-21 AU AU2002220754A patent/AU2002220754A1/en not_active Abandoned
Non-Patent Citations (1)
Title |
---|
CAYO RAMOS et al. "A Bioluminiscent derivative of Pseudomonas Putida KT 2440 for deliberate release into the environment", FEMS MICROBIOLOGY ECOLOGY, mayo 2000, Volumen 34, 2; páginas 91-102. * |
Also Published As
Publication number | Publication date |
---|---|
WO2002042476A1 (es) | 2002-05-30 |
AU2002220754A1 (en) | 2002-06-03 |
ES2178576A1 (es) | 2002-12-16 |
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