ES2178517B1 - Procedimiento para el estudio de la activacion funcional de leucocitos, plaquetas y otras celulas. - Google Patents
Procedimiento para el estudio de la activacion funcional de leucocitos, plaquetas y otras celulas.Info
- Publication number
- ES2178517B1 ES2178517B1 ES009901181A ES9901181A ES2178517B1 ES 2178517 B1 ES2178517 B1 ES 2178517B1 ES 009901181 A ES009901181 A ES 009901181A ES 9901181 A ES9901181 A ES 9901181A ES 2178517 B1 ES2178517 B1 ES 2178517B1
- Authority
- ES
- Spain
- Prior art keywords
- procedure
- study
- cells
- functional activation
- platquets
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/80—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood groups or blood types or red blood cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56966—Animal cells
- G01N33/56972—White blood cells
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Hematology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Cell Biology (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Food Science & Technology (AREA)
- Pathology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Microbiology (AREA)
- Zoology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
Procedimiento para el estudio de la activación funcional de leucocitos, plaquetas y otras células, producida in vivo o inducida in vitro, basada en la estabilización de proteínas de membrana citoplasmática y su detección mediante técnicas de citometría cuantitativa sin manipulación adicional de la muestra que comprende las etapas de: a) incubar la muestra de forma secuencial con un inhibidor o una mezcla de inhibidores específicos de proteasas y con una mezcla de anticuerpos conjugados directamente con fluorocromos; b) medir mediante citometría cuantitativa las emisiones fluorescentes de los fluorocromos unidos a las células a través de anticuerpos, c) analizar los resultados obtenidos mediante análisis multiparamétrico para identificar las subpoblaciones celulares de interés y dentro de ellas las que expresan las proteínas de membrana estabilizadas cuantificando su expresión de forma objetiva en base a la intensidad de fluorescencia detectada.
Priority Applications (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES009901181A ES2178517B1 (es) | 1999-05-31 | 1999-05-31 | Procedimiento para el estudio de la activacion funcional de leucocitos, plaquetas y otras celulas. |
DE60024923T DE60024923T2 (de) | 1999-05-31 | 2000-05-03 | Verfahren zur Analyse der funktionellen Aktivierung von Leukocyten, Plättchen und anderen Zellen |
ES00500083T ES2255482T3 (es) | 1999-05-31 | 2000-05-03 | Procedimientos para el analisis de la activacion funcional de leucocitos, plaquetas y otras celulas. |
AT00500083T ATE313800T1 (de) | 1999-05-31 | 2000-05-03 | Verfahren zur analyse der funktionellen aktivierung von leukocyten, plättchen und anderen zellen |
EP00500083A EP1063526B1 (en) | 1999-05-31 | 2000-05-03 | Procedure for the analysis of the functional activation of leukocytes, platelets and other cells |
US09/583,172 US6667149B1 (en) | 1999-05-31 | 2000-05-30 | Procedure for the analysis of the functional activation of leukocytes, platelets and other cells, induced in vivo or in vitro, based on the stabilization of cytoplasmic membrane proteins and its detection using quantitative cytometric methods in the absence of additional sample manipulation |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES009901181A ES2178517B1 (es) | 1999-05-31 | 1999-05-31 | Procedimiento para el estudio de la activacion funcional de leucocitos, plaquetas y otras celulas. |
Publications (2)
Publication Number | Publication Date |
---|---|
ES2178517A1 ES2178517A1 (es) | 2002-12-16 |
ES2178517B1 true ES2178517B1 (es) | 2004-09-16 |
Family
ID=8308633
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES009901181A Expired - Fee Related ES2178517B1 (es) | 1999-05-31 | 1999-05-31 | Procedimiento para el estudio de la activacion funcional de leucocitos, plaquetas y otras celulas. |
ES00500083T Expired - Lifetime ES2255482T3 (es) | 1999-05-31 | 2000-05-03 | Procedimientos para el analisis de la activacion funcional de leucocitos, plaquetas y otras celulas. |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES00500083T Expired - Lifetime ES2255482T3 (es) | 1999-05-31 | 2000-05-03 | Procedimientos para el analisis de la activacion funcional de leucocitos, plaquetas y otras celulas. |
Country Status (5)
Country | Link |
---|---|
US (1) | US6667149B1 (es) |
EP (1) | EP1063526B1 (es) |
AT (1) | ATE313800T1 (es) |
DE (1) | DE60024923T2 (es) |
ES (2) | ES2178517B1 (es) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2260942A3 (en) * | 2002-05-13 | 2011-03-09 | Becton, Dickinson and Company | Protease Inhibitor Sample Collection System |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ATE151546T1 (de) * | 1991-08-28 | 1997-04-15 | Becton Dickinson Co | Schwerkraftsattraktionsmaschine zur anpassungsfähigen autoclusterbildung n- dimensionaler datenströme |
US5710262A (en) * | 1992-04-17 | 1998-01-20 | Dana-Faber Cancer Institute, Inc. | Nucleic acid encoding HB15 polypeptides |
CZ283972B6 (cs) * | 1995-05-17 | 1998-07-15 | František Mudr. Trnka | Farmaceutický prostředek s modulačním účinkem na zhoubné nádory a jeho použití |
US5981180A (en) * | 1995-10-11 | 1999-11-09 | Luminex Corporation | Multiplexed analysis of clinical specimens apparatus and methods |
-
1999
- 1999-05-31 ES ES009901181A patent/ES2178517B1/es not_active Expired - Fee Related
-
2000
- 2000-05-03 AT AT00500083T patent/ATE313800T1/de not_active IP Right Cessation
- 2000-05-03 DE DE60024923T patent/DE60024923T2/de not_active Expired - Lifetime
- 2000-05-03 EP EP00500083A patent/EP1063526B1/en not_active Expired - Lifetime
- 2000-05-03 ES ES00500083T patent/ES2255482T3/es not_active Expired - Lifetime
- 2000-05-30 US US09/583,172 patent/US6667149B1/en not_active Expired - Lifetime
Non-Patent Citations (4)
Title |
---|
DEBETS J. et al.: "Cross-linking of both Fc & RI and Fc & RII induces secretion of tumor necrosis factor by human monocytes, requiring high affinity Fc-Fc & R interactions". 1990. JOURNAL OF IMMUNOLOGY. Vol. 144 (4). Páginas 1304-1310. * |
MITTRUECKER HANS-WILL CHRISTIANE STEEG et al.: "The cytoplasmic tail of the T cell receptor zeta chain is required for signaling via CD26". 1995. EUR. J. IMMUNOL. Vol. 25 (1). Páginas 295-297. * |
REMOLD-O'DONNELL EILEEN et al.: "Down regulation of Neutrophil CD43 by Opronited Zymosan". 1995. BLOOD. Vol. 85 (2). Páginas 337-342. * |
REMOLD-O'DONNELL EILEEN et al.: "Two proteolitic pathways for down-regulation of the barrier molecular CD43 of human neutrophils". 1994. JOURNAL OF IMMUNOLOGY. VOL. 152 (7). Páginas 3595-3605. * |
Also Published As
Publication number | Publication date |
---|---|
EP1063526A2 (en) | 2000-12-27 |
ES2178517A1 (es) | 2002-12-16 |
DE60024923D1 (de) | 2006-01-26 |
EP1063526B1 (en) | 2005-12-21 |
DE60024923T2 (de) | 2006-08-31 |
US6667149B1 (en) | 2003-12-23 |
ES2255482T3 (es) | 2006-07-01 |
EP1063526A3 (en) | 2002-07-31 |
ATE313800T1 (de) | 2006-01-15 |
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Legal Events
Date | Code | Title | Description |
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EC2A | Search report published |
Date of ref document: 20021216 Kind code of ref document: A1 |
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FD2A | Announcement of lapse in spain |
Effective date: 20190926 |