ES2170107T3 - Nueva cefalosporina c-acilasa. - Google Patents

Nueva cefalosporina c-acilasa.

Info

Publication number
ES2170107T3
ES2170107T3 ES94931165T ES94931165T ES2170107T3 ES 2170107 T3 ES2170107 T3 ES 2170107T3 ES 94931165 T ES94931165 T ES 94931165T ES 94931165 T ES94931165 T ES 94931165T ES 2170107 T3 ES2170107 T3 ES 2170107T3
Authority
ES
Spain
Prior art keywords
acylase
pct
date
amino acid
mutant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES94931165T
Other languages
English (en)
Inventor
Mineo Niwa
Yoshimasa Saito
Takao Fujimura
Yoshinori Ishii
Yuji Noguchi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fujisawa Pharmaceutical Co Ltd
Original Assignee
Fujisawa Pharmaceutical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB939322508A external-priority patent/GB9322508D0/en
Priority claimed from GB9326519A external-priority patent/GB9326519D0/en
Application filed by Fujisawa Pharmaceutical Co Ltd filed Critical Fujisawa Pharmaceutical Co Ltd
Application granted granted Critical
Publication of ES2170107T3 publication Critical patent/ES2170107T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P35/00—Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin
    • C12P35/02—Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin by desacylation of the substituent in the 7 position
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Cephalosporin Compounds (AREA)

Abstract

SE PRESENTA UNA ACILASA CC MUTANTE EN LA QUE AL MENOS UN AMINOACIDO EN LA POSICION ALA{SUP,49}, MET{SUP,164}, SER{SUP,166}, MET{SUP,174}, GLU{SUP,465}, MET{SUP,506}, O MET{SUP,750} DE LA SECUENCIA DE AMINOACIDOS DE LA ACILASA CC NATIVA SE REEMPLAZA POR UN AMINOACIDO DIFERENTE. TAMBIEN SE PRESENTAN UN ADN QUE LA CODIFICA, UN VECTOR DE EXPRESION QUE CONTIENE EL MENCIONADO ADN, UN MICROORGANISMO TRANSFORMADO CON EL VECTOR DE EXPRESION, LA PRODUCCION DE LA ACILASA CC CULTIVANDO EL TRANSFORMANTE, Y EL USO DE LA MISMA PARA LA PRODUCCION DE UN COMPUESTO. LA ACILASA CC MUTANTE DE LA INVENCION TIENE PROPIEDADES DESEABLES EN TERMINOS DE POTENCIA ENZIMATICA, LA ACELERACION DEL PERFIL DEL PH, EFICIENCIA DE PROCESAMIENTO Y SIMILARES.
ES94931165T 1993-11-01 1994-10-26 Nueva cefalosporina c-acilasa. Expired - Lifetime ES2170107T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB939322508A GB9322508D0 (en) 1993-11-01 1993-11-01 A new cephalosporin c acylase
GB9326519A GB9326519D0 (en) 1993-12-29 1993-12-29 A new cephalosporin C acylase

Publications (1)

Publication Number Publication Date
ES2170107T3 true ES2170107T3 (es) 2002-08-01

Family

ID=26303776

Family Applications (1)

Application Number Title Priority Date Filing Date
ES94931165T Expired - Lifetime ES2170107T3 (es) 1993-11-01 1994-10-26 Nueva cefalosporina c-acilasa.

Country Status (12)

Country Link
US (1) US5804429A (es)
EP (1) EP0726958B1 (es)
JP (1) JPH09505732A (es)
KR (1) KR960705932A (es)
CN (1) CN1150823A (es)
AT (1) ATE214098T1 (es)
CA (1) CA2175338A1 (es)
DE (1) DE69430067T2 (es)
ES (1) ES2170107T3 (es)
HU (1) HUT76084A (es)
TW (1) TW400384B (es)
WO (1) WO1995012680A1 (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0453048B1 (en) * 1990-04-18 1998-06-17 Gist-Brocades N.V. Mutated beta-lactam acylase genes
US5891703A (en) * 1994-08-12 1999-04-06 Gist-Hrocades Mutated penicillin G acylase genes
ATE402997T1 (de) 1996-11-05 2008-08-15 Bristol Myers Squibb Co Mutierte penicillin g acylasen
AU2002310863A1 (en) 2001-04-19 2002-11-05 Bioferma Murcia, S.A. Enzymatic process for preparing cephalosporanic acid derivatives using alpha-ketoacid derivatives
KR100530299B1 (ko) * 2003-08-11 2005-11-22 산도즈 게엠베하 변이 세팔로스포린 ｃ 아실라제 및 이를 이용한 7-ａｃａ 제조방법
ITMI20040016A1 (it) * 2004-01-12 2004-04-12 Antibiotics S P A Cefalosporina c acilasi
WO2007073947A2 (en) 2005-12-28 2007-07-05 Dsm Ip Assets B.V. Mutant type ii beta- lactam acylases

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9019724D0 (en) * 1990-09-10 1990-10-24 Fujisawa Pharmaceutical Co Cephalosporin c acylase
GB9022907D0 (en) * 1990-10-22 1990-12-05 Fujisawa Pharmaceutical Co Novel gl-7aca acylase
GB9204439D0 (en) * 1992-02-27 1992-04-15 Fujisawa Pharmaceutical Co A new cephalosporin c acylase

Also Published As

Publication number Publication date
HUT76084A (en) 1997-06-30
EP0726958B1 (en) 2002-03-06
CA2175338A1 (en) 1995-05-11
ATE214098T1 (de) 2002-03-15
JPH09505732A (ja) 1997-06-10
DE69430067D1 (de) 2002-04-11
WO1995012680A1 (en) 1995-05-11
TW400384B (en) 2000-08-01
EP0726958A1 (en) 1996-08-21
KR960705932A (ko) 1996-11-08
CN1150823A (zh) 1997-05-28
HU9601151D0 (en) 1996-06-28
US5804429A (en) 1998-09-08
DE69430067T2 (de) 2002-08-14

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