ES2168358T3 - Proteinas antimicrobianas de semillas de impatiens. - Google Patents

Proteinas antimicrobianas de semillas de impatiens.

Info

Publication number
ES2168358T3
ES2168358T3 ES95910649T ES95910649T ES2168358T3 ES 2168358 T3 ES2168358 T3 ES 2168358T3 ES 95910649 T ES95910649 T ES 95910649T ES 95910649 T ES95910649 T ES 95910649T ES 2168358 T3 ES2168358 T3 ES 2168358T3
Authority
ES
Spain
Prior art keywords
pct
proteins
impatiens
seeds
dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES95910649T
Other languages
English (en)
Inventor
Sheila Attenborough
Willem Frans Broekaert
Rupert William Osborn
John Anthony Ray
Sarah Bronwen Rees
Ravindra Haribhai Tailor
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Syngenta Ltd
Original Assignee
Syngenta Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Syngenta Ltd filed Critical Syngenta Ltd
Application granted granted Critical
Publication of ES2168358T3 publication Critical patent/ES2168358T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8282—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for fungal resistance
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8281—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for bacterial resistance
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Botany (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Agronomy & Crop Science (AREA)
  • Mycology (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Communicable Diseases (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Oncology (AREA)
  • Peptides Or Proteins (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

LAS PROTEINAS ANTIMICROBIANAS CAPACES DEL AISLAMIENTO DE SEMILLAS DE ARALIA O IMPATIENS MUESTRAN UNA VARIACION AMPLIA DE ACTIVIDAD ANTIFUNGICA Y ALGUNA ACTIVIDAD ANTIBACTERIANA. EL ADN QUE CODIFICA LAS PROTEINAS PUEDE SER AISLADO E INCORPORADO EN VECTORES. LAS PLANTAS TRANSFORMADAS CON ESTE ADN PUEDEN SER PRODUCIDAS. LAS PROTEINAS ENCUENTRAN APLICACION COMERCIAL COMO AGENTES ANTIFUNGICOS O ANTIBACTERIANOS; LAS PLANTAS TRANSFORMADAS MOSTRARAN UNA AUMENTADA RESISTENCIA A LAS ENFERMEDADES AUNMENTADA. LA INVENCION ADEMAS SUMINISTRA UN METODO PARA LA EXPRESION DE POLIPROTEINAS EN PLANTAS TRANSGENICAS USANDO CONSTRUCCIONES DE ADN BASADAS EN LA ESTRUCTURA DEL GEN QUE CODIFICA LAS PROTEINAS ANTIMICROBIANAS IMPATIENS.
ES95910649T 1994-03-11 1995-03-09 Proteinas antimicrobianas de semillas de impatiens. Expired - Lifetime ES2168358T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB9404807A GB9404807D0 (en) 1994-03-11 1994-03-11 Biocidal proteins

Publications (1)

Publication Number Publication Date
ES2168358T3 true ES2168358T3 (es) 2002-06-16

Family

ID=10751721

Family Applications (1)

Application Number Title Priority Date Filing Date
ES95910649T Expired - Lifetime ES2168358T3 (es) 1994-03-11 1995-03-09 Proteinas antimicrobianas de semillas de impatiens.

Country Status (14)

Country Link
US (2) US5861480A (es)
EP (1) EP0749485B1 (es)
JP (1) JPH09509844A (es)
CN (2) CN1143978A (es)
AT (1) ATE213506T1 (es)
AU (1) AU685920B2 (es)
BR (1) BR9507034A (es)
CA (1) CA2180353A1 (es)
DE (1) DE69525529T2 (es)
DK (1) DK0749485T3 (es)
ES (1) ES2168358T3 (es)
GB (1) GB9404807D0 (es)
PT (1) PT749485E (es)
WO (1) WO1995024486A1 (es)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997021815A2 (en) * 1995-12-13 1997-06-19 Zeneca Limited Antifungal proteins
US6121436A (en) 1996-12-13 2000-09-19 Monsanto Company Antifungal polypeptide and methods for controlling plant pathogenic fungi
GB9714564D0 (en) 1997-07-10 1997-09-17 Zeneca Ltd Composition
US6121511A (en) * 1997-09-12 2000-09-19 Ball Horticultural Company Production of transgenic impatiens
BR9913076A (pt) * 1998-08-18 2001-05-08 Zeneca Ltd Método para melhorar nìveis de expressão de uma ou mais proteìnas em um vegetal transgênico, e para a expressão de proteìnas múltiplas em um vegetal transgênico, uso de um propeptìdeo clivável no caminho secretor de um ligador vegetal, e como um ligador clivável em precusores de poliproteìna sintetizados por intermédio do caminho secretor em vegetais transgênicos, uso em uma sequência de propeptìdeo rica nos aminoácidos pequenos a, v, s e t e contendo sequências dipeptìdicas consistindo de dois resìduos ácidos, dois resìduos básicos ou um resìduo ácido e um básico como uma sequência ligadora clivável, construção de dna, vetor, vegetal transgênico, uso de uma construção de dna, e, ácido nucleico
US6528703B1 (en) 1998-09-11 2003-03-04 Ball Horticultural Company Production of transgenic impatiens
US6855865B2 (en) * 1999-05-07 2005-02-15 E.I. Du Pont De Nemours And Company Nucleic acids encoding plant defensins and methods of use thereof
GB9911933D0 (en) * 1999-05-21 1999-07-21 Zeneca Ltd Composition
US20020170089A1 (en) * 2001-02-28 2002-11-14 Millennium Pharmaceuticals Nucleic acids encoding defense inducible proteins and uses thereof
EP2270187A3 (en) * 2001-06-22 2011-08-31 Pioneer Hi-Bred International, Inc. Defensin polynucleotides and methods of use
FR2841903B1 (fr) 2002-07-05 2004-09-24 Centre Nat Rech Scient Nouveau peptide de plante a activite anti-microbienne
US20110078820A1 (en) * 2009-09-30 2011-03-31 Clemson University Research Foundation Methods and compositions for transgenic plants producing antimicrobial peptides for enhanced disease resistance
US8945876B2 (en) 2011-11-23 2015-02-03 University Of Hawaii Auto-processing domains for polypeptide expression
KR102358469B1 (ko) * 2021-08-23 2022-02-08 브이앤코 주식회사 물봉선 유래 항균 펩타이드 및 이를 함유하는 항균용 조성물

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5597945A (en) * 1986-07-25 1997-01-28 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Plants genetically enhanced for disease resistance
US5162601A (en) * 1989-11-22 1992-11-10 The Upjohn Company Plant potyvirus expression vector with a gene for protease
GB9112300D0 (en) * 1991-06-07 1991-07-24 Ici Plc Biocidal proteins

Also Published As

Publication number Publication date
JPH09509844A (ja) 1997-10-07
ATE213506T1 (de) 2002-03-15
PT749485E (pt) 2002-06-28
CN1313387A (zh) 2001-09-19
DK0749485T3 (da) 2002-03-18
US6150588A (en) 2000-11-21
CA2180353A1 (en) 1995-09-14
EP0749485B1 (en) 2002-02-20
DE69525529T2 (de) 2002-08-22
WO1995024486A1 (en) 1995-09-14
DE69525529D1 (de) 2002-03-28
CN1143978A (zh) 1997-02-26
EP0749485A1 (en) 1996-12-27
GB9404807D0 (en) 1994-04-27
US5861480A (en) 1999-01-19
BR9507034A (pt) 1997-09-02
AU685920B2 (en) 1998-01-29
AU1856295A (en) 1995-09-25

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