ES2161130A1 - Dna recombinante derivado del virus de la sharka y vector de expresion de proteinas heterologas basado en dicho dna recombinante. - Google Patents

Dna recombinante derivado del virus de la sharka y vector de expresion de proteinas heterologas basado en dicho dna recombinante.

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Publication number
ES2161130A1
ES2161130A1 ES9900698A ES9900698A ES2161130A1 ES 2161130 A1 ES2161130 A1 ES 2161130A1 ES 9900698 A ES9900698 A ES 9900698A ES 9900698 A ES9900698 A ES 9900698A ES 2161130 A1 ES2161130 A1 ES 2161130A1
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ES
Spain
Prior art keywords
undefined
sharka
amino acids
recombinant dna
dna derived
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES9900698A
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English (en)
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ES2161130B1 (es
Inventor
Fernandez M Del Rosa Fernandez
Gomez Juan Jose Lopez-Moya
Alvarez Juan Antonio Garcia
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Consejo Superior de Investigaciones Cientificas CSIC
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Consejo Superior de Investigaciones Cientificas CSIC
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Application filed by Consejo Superior de Investigaciones Cientificas CSIC filed Critical Consejo Superior de Investigaciones Cientificas CSIC
Priority to ES9900698A priority Critical patent/ES2161130B1/es
Publication of ES2161130A1 publication Critical patent/ES2161130A1/es
Application granted granted Critical
Publication of ES2161130B1 publication Critical patent/ES2161130B1/es
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

DNA recombinante derivado del virus de la sharka y vector de expresión de proteínas heterólogas basado en dicho DNA recombinante. El DNA recombinante comprende un cDNA al genoma del virus de la shaharka (PPV), de longitud completa, que contiene entre las secuencias codificantes de las proteínas Nib y CP: a) una secuencia ajena seleccionada entre (i) una secuencia de 18 nucleótidos que codifican a los 6 primeros aminoácidos de un heptapéptido de reconocimiento de la proteasa Nia de PPV, y (ii) una secuencia de 21 nucleótidos que codifica a un heptapéptido de reconocimiento de la proteasa Nia de PPV; b) una diana para una primera enzima de restricción; c) una secuencia espaciadora; y d) una dian para una segunda enzima de restricción. El vector comprende un DNA recombinante que contiene una secuencia ajena insertada entre las secuencias codificantes de las proteínas Nib y CP y es útil para expresar proteínas heterólogas en plantas.
ES9900698A 1999-04-07 1999-04-07 Dna recombinante derivado del virus de la sharka y vector de expresion de proteinas heterologas basado en dicho dna recombinante. Expired - Fee Related ES2161130B1 (es)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES9900698A ES2161130B1 (es) 1999-04-07 1999-04-07 Dna recombinante derivado del virus de la sharka y vector de expresion de proteinas heterologas basado en dicho dna recombinante.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES9900698A ES2161130B1 (es) 1999-04-07 1999-04-07 Dna recombinante derivado del virus de la sharka y vector de expresion de proteinas heterologas basado en dicho dna recombinante.

Publications (2)

Publication Number Publication Date
ES2161130A1 true ES2161130A1 (es) 2001-11-16
ES2161130B1 ES2161130B1 (es) 2002-06-16

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Family Applications (1)

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ES9900698A Expired - Fee Related ES2161130B1 (es) 1999-04-07 1999-04-07 Dna recombinante derivado del virus de la sharka y vector de expresion de proteinas heterologas basado en dicho dna recombinante.

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ES (1) ES2161130B1 (es)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2331550A1 (es) * 2004-11-16 2010-01-07 Consejo Superior De Investigaciones Cientificas Procedimiento para la deteccion simultanea de virus mediante el uso de polisondas.
WO2011110713A1 (es) * 2010-03-09 2011-09-15 Consejo Superior De Investigaciones Científicas (Csic) Vector para la coexpresión de varias proteínas heterólogas en cantidades equimolares

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5162601A (en) * 1989-11-22 1992-11-10 The Upjohn Company Plant potyvirus expression vector with a gene for protease
WO1995021249A1 (en) * 1994-02-03 1995-08-10 The Scripps Research Institute A cassette to accumulate multiple proteins through synthesis of a self-processing polypeptide
US5766885A (en) * 1993-11-01 1998-06-16 Texas A & M University Potyvirus vectors for the expression of foreign genes

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5162601A (en) * 1989-11-22 1992-11-10 The Upjohn Company Plant potyvirus expression vector with a gene for protease
US5766885A (en) * 1993-11-01 1998-06-16 Texas A & M University Potyvirus vectors for the expression of foreign genes
WO1995021249A1 (en) * 1994-02-03 1995-08-10 The Scripps Research Institute A cassette to accumulate multiple proteins through synthesis of a self-processing polypeptide

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
GUO et al. "Susceptibility to recombination reagements of a chimeric plum pox potyvirus genome after insertion of a foreign gene", 1998. Virus Research, Vol. 57, paginas 183-195. *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2331550A1 (es) * 2004-11-16 2010-01-07 Consejo Superior De Investigaciones Cientificas Procedimiento para la deteccion simultanea de virus mediante el uso de polisondas.
WO2011110713A1 (es) * 2010-03-09 2011-09-15 Consejo Superior De Investigaciones Científicas (Csic) Vector para la coexpresión de varias proteínas heterólogas en cantidades equimolares
ES2370673A1 (es) * 2010-03-09 2011-12-21 Consejo Superior De Investigaciones Científicas (Csic) Vector para la coexpresión de varias proteínas heterólogas en cantidades equimolares.

Also Published As

Publication number Publication date
ES2161130B1 (es) 2002-06-16

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