ES2136389T3 - Procedimiento para la deteccion temprana de carcinomas asociados con el hpv o bien displasias intensas causadas por el hpv. - Google Patents

Procedimiento para la deteccion temprana de carcinomas asociados con el hpv o bien displasias intensas causadas por el hpv.

Info

Publication number
ES2136389T3
ES2136389T3 ES96904706T ES96904706T ES2136389T3 ES 2136389 T3 ES2136389 T3 ES 2136389T3 ES 96904706 T ES96904706 T ES 96904706T ES 96904706 T ES96904706 T ES 96904706T ES 2136389 T3 ES2136389 T3 ES 2136389T3
Authority
ES
Spain
Prior art keywords
hpv
procedure
cdna
dysplasias
caused
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES96904706T
Other languages
English (en)
Inventor
Knebel-Doberitz Magnus Von
Stefan Worner
Florian Emmerich
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Deutsches Krebsforschungszentrum DKFZ
Original Assignee
Deutsches Krebsforschungszentrum DKFZ
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Deutsches Krebsforschungszentrum DKFZ filed Critical Deutsches Krebsforschungszentrum DKFZ
Application granted granted Critical
Publication of ES2136389T3 publication Critical patent/ES2136389T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/708—Specific hybridization probes for papilloma

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

LA INVENCION TRATA DE UN PROCEDIMIENTO PARA IDENTIFICAR MRNA, QUE TIENE UNAS SECUENCIAS HPV Y UNAS SECUENCIAS CELULARES, EN UNA MUESTRA CORPORAL, QUE COMPRENDE LAS SIGUIENTES FASES: (A) TOMA Y PREPARACION DE UNA MUESTRA CORPORAL; (B) AISLAMIENTO DEL MRNA DE LA MUESTRA CORPORAL DE (A); (C) TRADUCCION DEL MRNA DE (B) A CDNA UTILIZANDO UN PRIMER NORMAL PARA UNA TRANSCRIPCION INVERSA; (D) AMPLIFICACION DEL CDNA DE (C) MEDIANTE UNA REACCION PCR CON UN PRIMERCION DEL CDNA AMPLIFICADO DE (D) CON UNA ENDONUCLEASA SEPARADA EN EL LADO 5'' DE LA SECUENCIA DE POLIADENILIZACION DEL HPV; (F) AMPLIFICACION DEL CDNA NO SEPARADO DE (E) CON LOS PRIMERS DE (D) O CON UNOS PRIMERS "NESTED", Y (G) IDENTIFICACION DEL CDNA AMPLIFICADO DE (F). LA INVENCION SE REFIERE TAMBIEN A LA UTILIZACION DE DICHO PROCEDIMIENTO PARA EL RECONOCIMIENTO PRECOZ DE CARCINOMAS ASOCIADOS AL HPV O DE DISPLASIAS EXTREMAS CAUSADAS POR EL HPV.
ES96904706T 1995-02-24 1996-02-23 Procedimiento para la deteccion temprana de carcinomas asociados con el hpv o bien displasias intensas causadas por el hpv. Expired - Lifetime ES2136389T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19506561A DE19506561C1 (de) 1995-02-24 1995-02-24 Verfahren zur Früherkennung von HPV-assoziierten Karzinomen bzw. von hochgradigen, durch HPV-verursachten Dysplasien

Publications (1)

Publication Number Publication Date
ES2136389T3 true ES2136389T3 (es) 1999-11-16

Family

ID=7754994

Family Applications (1)

Application Number Title Priority Date Filing Date
ES96904706T Expired - Lifetime ES2136389T3 (es) 1995-02-24 1996-02-23 Procedimiento para la deteccion temprana de carcinomas asociados con el hpv o bien displasias intensas causadas por el hpv.

Country Status (8)

Country Link
US (1) US6027891A (es)
EP (1) EP0811079B1 (es)
JP (1) JPH11500314A (es)
AT (1) ATE180020T1 (es)
DE (2) DE19506561C1 (es)
DK (1) DK0811079T3 (es)
ES (1) ES2136389T3 (es)
WO (1) WO1996026293A2 (es)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2183695A1 (es) * 2000-10-31 2003-03-16 Ivia Metodo y mezcla de reaccion para la amplificacion enzimatica simultanea, coordinada y cooperativa de secuencias diana de acidos nucleicos.

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19649207C1 (de) * 1996-11-27 1998-02-26 Deutsches Krebsforsch DNA, Polypeptid und Antikörper zur Abschätzung des Progressionspotentials von Zervixläsionen
GB0200258D0 (en) * 2002-01-07 2002-02-20 Norchip As Detection of human papillomavirus
DK2913407T3 (en) * 2002-01-07 2018-01-15 Pretect As Method for detecting human papillomavirus mRNA
CA2550900A1 (en) 2003-12-23 2005-07-07 Mount Sinai Hospital Methods for detecting markers associated with endometrial disease or phase
GB0404315D0 (en) * 2004-02-26 2004-03-31 Norchip As Improved detection of human papillomavirus
CA2588581C (en) 2004-12-08 2011-05-31 Gen-Probe Incorporated Detection of nucleic acids from multiple types of human papillomaviruses
US20070031826A1 (en) * 2005-08-05 2007-02-08 My Gene Diagnostic kit for determining the genotype of a human papilloma virus and method of using thereof
WO2011109705A2 (en) * 2010-03-04 2011-09-09 Purdue Research Foundation Integrated assay that combines flow-cytometry and multiplexed hpv genotype identification
EP2576840B1 (en) 2010-05-25 2018-10-17 QIAGEN Gaithersburg, Inc. Fast results hybrid capture assay and associated strategically-truncated probes
EP2580355B1 (en) * 2010-06-14 2018-03-07 National University of Singapore Modified stem-loop oligonucleotide mediated reverse transcription and base-spacing constrained quantitative pcr

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3096704B2 (ja) * 1987-02-26 2000-10-10 バイオサーチ インターナショナル ピティ リミテッド ヒトの発癌性乳頭腫ウイルスの検出方法
DE3838269A1 (de) * 1988-11-11 1990-05-17 Behringwerke Ag Nachweis humaner papillomavirus dna und ihrer expression in zervix-abstrichen
JP2791685B2 (ja) * 1989-06-08 1998-08-27 寳酒造株式会社 パピローマウイルスの検出方法
DE69033850T2 (de) * 1989-12-01 2002-06-06 Vysis, Inc. Nukleinsäuresonden zum Nachweis von HPV-Transkripts
EP0466367B1 (en) * 1990-06-28 1995-08-23 Wakunaga Seiyaku Kabushiki Kaisha Process for detecting nucleic acid
CA2139623A1 (en) * 1993-05-06 1994-11-24 Baxter Diagnostics Inc. Human papillomavirus detection assay
US5821048B1 (en) * 1995-06-07 2000-05-23 Harvard College Methods kits and compositions for diagnosing papillomavirus infection

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2183695A1 (es) * 2000-10-31 2003-03-16 Ivia Metodo y mezcla de reaccion para la amplificacion enzimatica simultanea, coordinada y cooperativa de secuencias diana de acidos nucleicos.

Also Published As

Publication number Publication date
EP0811079B1 (de) 1999-05-12
WO1996026293A3 (de) 1996-12-12
US6027891A (en) 2000-02-22
DE59601881D1 (de) 1999-06-17
ATE180020T1 (de) 1999-05-15
DE19506561C1 (de) 1996-10-10
JPH11500314A (ja) 1999-01-12
EP0811079A2 (de) 1997-12-10
WO1996026293A2 (de) 1996-08-29
DK0811079T3 (da) 1999-11-15

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