ES2136389T3 - Procedimiento para la deteccion temprana de carcinomas asociados con el hpv o bien displasias intensas causadas por el hpv. - Google Patents
Procedimiento para la deteccion temprana de carcinomas asociados con el hpv o bien displasias intensas causadas por el hpv.Info
- Publication number
- ES2136389T3 ES2136389T3 ES96904706T ES96904706T ES2136389T3 ES 2136389 T3 ES2136389 T3 ES 2136389T3 ES 96904706 T ES96904706 T ES 96904706T ES 96904706 T ES96904706 T ES 96904706T ES 2136389 T3 ES2136389 T3 ES 2136389T3
- Authority
- ES
- Spain
- Prior art keywords
- hpv
- procedure
- cdna
- dysplasias
- caused
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
- C12Q1/708—Specific hybridization probes for papilloma
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Virology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
LA INVENCION TRATA DE UN PROCEDIMIENTO PARA IDENTIFICAR MRNA, QUE TIENE UNAS SECUENCIAS HPV Y UNAS SECUENCIAS CELULARES, EN UNA MUESTRA CORPORAL, QUE COMPRENDE LAS SIGUIENTES FASES: (A) TOMA Y PREPARACION DE UNA MUESTRA CORPORAL; (B) AISLAMIENTO DEL MRNA DE LA MUESTRA CORPORAL DE (A); (C) TRADUCCION DEL MRNA DE (B) A CDNA UTILIZANDO UN PRIMER NORMAL PARA UNA TRANSCRIPCION INVERSA; (D) AMPLIFICACION DEL CDNA DE (C) MEDIANTE UNA REACCION PCR CON UN PRIMERCION DEL CDNA AMPLIFICADO DE (D) CON UNA ENDONUCLEASA SEPARADA EN EL LADO 5'' DE LA SECUENCIA DE POLIADENILIZACION DEL HPV; (F) AMPLIFICACION DEL CDNA NO SEPARADO DE (E) CON LOS PRIMERS DE (D) O CON UNOS PRIMERS "NESTED", Y (G) IDENTIFICACION DEL CDNA AMPLIFICADO DE (F). LA INVENCION SE REFIERE TAMBIEN A LA UTILIZACION DE DICHO PROCEDIMIENTO PARA EL RECONOCIMIENTO PRECOZ DE CARCINOMAS ASOCIADOS AL HPV O DE DISPLASIAS EXTREMAS CAUSADAS POR EL HPV.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE19506561A DE19506561C1 (de) | 1995-02-24 | 1995-02-24 | Verfahren zur Früherkennung von HPV-assoziierten Karzinomen bzw. von hochgradigen, durch HPV-verursachten Dysplasien |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2136389T3 true ES2136389T3 (es) | 1999-11-16 |
Family
ID=7754994
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES96904706T Expired - Lifetime ES2136389T3 (es) | 1995-02-24 | 1996-02-23 | Procedimiento para la deteccion temprana de carcinomas asociados con el hpv o bien displasias intensas causadas por el hpv. |
Country Status (8)
Country | Link |
---|---|
US (1) | US6027891A (es) |
EP (1) | EP0811079B1 (es) |
JP (1) | JPH11500314A (es) |
AT (1) | ATE180020T1 (es) |
DE (2) | DE19506561C1 (es) |
DK (1) | DK0811079T3 (es) |
ES (1) | ES2136389T3 (es) |
WO (1) | WO1996026293A2 (es) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ES2183695A1 (es) * | 2000-10-31 | 2003-03-16 | Ivia | Metodo y mezcla de reaccion para la amplificacion enzimatica simultanea, coordinada y cooperativa de secuencias diana de acidos nucleicos. |
Families Citing this family (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE19649207C1 (de) * | 1996-11-27 | 1998-02-26 | Deutsches Krebsforsch | DNA, Polypeptid und Antikörper zur Abschätzung des Progressionspotentials von Zervixläsionen |
GB0200258D0 (en) * | 2002-01-07 | 2002-02-20 | Norchip As | Detection of human papillomavirus |
DK2913407T3 (en) * | 2002-01-07 | 2018-01-15 | Pretect As | Method for detecting human papillomavirus mRNA |
CA2550900A1 (en) | 2003-12-23 | 2005-07-07 | Mount Sinai Hospital | Methods for detecting markers associated with endometrial disease or phase |
GB0404315D0 (en) * | 2004-02-26 | 2004-03-31 | Norchip As | Improved detection of human papillomavirus |
CA2588581C (en) | 2004-12-08 | 2011-05-31 | Gen-Probe Incorporated | Detection of nucleic acids from multiple types of human papillomaviruses |
US20070031826A1 (en) * | 2005-08-05 | 2007-02-08 | My Gene | Diagnostic kit for determining the genotype of a human papilloma virus and method of using thereof |
WO2011109705A2 (en) * | 2010-03-04 | 2011-09-09 | Purdue Research Foundation | Integrated assay that combines flow-cytometry and multiplexed hpv genotype identification |
EP2576840B1 (en) | 2010-05-25 | 2018-10-17 | QIAGEN Gaithersburg, Inc. | Fast results hybrid capture assay and associated strategically-truncated probes |
EP2580355B1 (en) * | 2010-06-14 | 2018-03-07 | National University of Singapore | Modified stem-loop oligonucleotide mediated reverse transcription and base-spacing constrained quantitative pcr |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP3096704B2 (ja) * | 1987-02-26 | 2000-10-10 | バイオサーチ インターナショナル ピティ リミテッド | ヒトの発癌性乳頭腫ウイルスの検出方法 |
DE3838269A1 (de) * | 1988-11-11 | 1990-05-17 | Behringwerke Ag | Nachweis humaner papillomavirus dna und ihrer expression in zervix-abstrichen |
JP2791685B2 (ja) * | 1989-06-08 | 1998-08-27 | 寳酒造株式会社 | パピローマウイルスの検出方法 |
DE69033850T2 (de) * | 1989-12-01 | 2002-06-06 | Vysis, Inc. | Nukleinsäuresonden zum Nachweis von HPV-Transkripts |
EP0466367B1 (en) * | 1990-06-28 | 1995-08-23 | Wakunaga Seiyaku Kabushiki Kaisha | Process for detecting nucleic acid |
CA2139623A1 (en) * | 1993-05-06 | 1994-11-24 | Baxter Diagnostics Inc. | Human papillomavirus detection assay |
US5821048B1 (en) * | 1995-06-07 | 2000-05-23 | Harvard College | Methods kits and compositions for diagnosing papillomavirus infection |
-
1995
- 1995-02-24 DE DE19506561A patent/DE19506561C1/de not_active Expired - Fee Related
-
1996
- 1996-02-23 US US08/913,547 patent/US6027891A/en not_active Expired - Fee Related
- 1996-02-23 DK DK96904706T patent/DK0811079T3/da active
- 1996-02-23 DE DE59601881T patent/DE59601881D1/de not_active Expired - Fee Related
- 1996-02-23 AT AT96904706T patent/ATE180020T1/de not_active IP Right Cessation
- 1996-02-23 EP EP96904706A patent/EP0811079B1/de not_active Expired - Lifetime
- 1996-02-23 JP JP8525305A patent/JPH11500314A/ja active Pending
- 1996-02-23 ES ES96904706T patent/ES2136389T3/es not_active Expired - Lifetime
- 1996-02-23 WO PCT/DE1996/000306 patent/WO1996026293A2/de active IP Right Grant
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ES2183695A1 (es) * | 2000-10-31 | 2003-03-16 | Ivia | Metodo y mezcla de reaccion para la amplificacion enzimatica simultanea, coordinada y cooperativa de secuencias diana de acidos nucleicos. |
Also Published As
Publication number | Publication date |
---|---|
EP0811079B1 (de) | 1999-05-12 |
WO1996026293A3 (de) | 1996-12-12 |
US6027891A (en) | 2000-02-22 |
DE59601881D1 (de) | 1999-06-17 |
ATE180020T1 (de) | 1999-05-15 |
DE19506561C1 (de) | 1996-10-10 |
JPH11500314A (ja) | 1999-01-12 |
EP0811079A2 (de) | 1997-12-10 |
WO1996026293A2 (de) | 1996-08-29 |
DK0811079T3 (da) | 1999-11-15 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG2A | Definitive protection |
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