ES2133275T3 - N-carbamoil-sarcosina-amidohidrolasa clonada. - Google Patents

N-carbamoil-sarcosina-amidohidrolasa clonada.

Info

Publication number
ES2133275T3
ES2133275T3 ES91115974T ES91115974T ES2133275T3 ES 2133275 T3 ES2133275 T3 ES 2133275T3 ES 91115974 T ES91115974 T ES 91115974T ES 91115974 T ES91115974 T ES 91115974T ES 2133275 T3 ES2133275 T3 ES 2133275T3
Authority
ES
Spain
Prior art keywords
sequence
dna
amidohidrolase
carbamoil
sarcosine
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES91115974T
Other languages
English (en)
Inventor
Helmut Dr Burtscher
Gunther Dr Schumacher
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Roche Diagnostics GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roche Diagnostics GmbH filed Critical Roche Diagnostics GmbH
Application granted granted Critical
Publication of ES2133275T3 publication Critical patent/ES2133275T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/83—Arthrobacter
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/848—Escherichia
    • Y10S435/849—Escherichia coli

Abstract

LA INVENCION TRATA DE UN DNA RECOMBINADO, QUE CONTIENE (1) LA SECUENCIA PRESENTADA EN SEQ ID NO: 1, (2) UNA SECUENCIA QUE CORRESPONDE A ESTA SECUENCIA EN EL TRANSCURSO DE LA DEGENERACION DEL CODIGO GENETICO O (3) UNA SECUENCIA QUE HIBRIDIZA CON UNA SECUENCIA DE (1) O/Y (2) EN CONDICIONES DE HIBRIDIZACION ESTRINGENTE, CON LO QUE LA SECUENCIA DNA SE CODIFICA PARA UNA PROTEINA CON UNA ACTIVIDAD N-CARBAMOILSARCOSINO-AMIDOHIDROLASO. ADEMAS LA INVENCION MUESTRA UN VECTOR RECOMBINADO CON EL DNA SEGUN LA INVENCION ASI COMO UN METODO PARA LA OBTENCION DE UNA PROTEINA RECOMBINADA CON ACTIVIDAD N-CARBAMOILSARCOSINO-AMIDOHIDROLASO Y SU APLICACION PARA LA DETERMINACION DE CREATININA.
ES91115974T 1990-09-20 1991-09-19 N-carbamoil-sarcosina-amidohidrolasa clonada. Expired - Lifetime ES2133275T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE4029844A DE4029844A1 (de) 1990-09-20 1990-09-20 Carbamoylsarcosinhydrolase, kloniert

Publications (1)

Publication Number Publication Date
ES2133275T3 true ES2133275T3 (es) 1999-09-16

Family

ID=6414642

Family Applications (1)

Application Number Title Priority Date Filing Date
ES91115974T Expired - Lifetime ES2133275T3 (es) 1990-09-20 1991-09-19 N-carbamoil-sarcosina-amidohidrolasa clonada.

Country Status (6)

Country Link
US (1) US5416014A (es)
EP (1) EP0476670B1 (es)
JP (1) JP2627587B2 (es)
AT (1) ATE180015T1 (es)
DE (2) DE4029844A1 (es)
ES (1) ES2133275T3 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0602173D0 (en) 2006-02-03 2006-03-15 Avecia Ltd Expression system

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL61982A (en) * 1980-02-15 1984-01-31 Cpc International Inc Genetically engineered microorganisms for massive production of amyloytic enzymes and process for preparing same using the corresponding recombinant dnas containing amylase coding genes
US4464473A (en) * 1982-04-23 1984-08-07 The Regents Of The University Of California Ice nucleating microorganisms
US4472502A (en) * 1982-08-16 1984-09-18 The Regents Of The University Of California Malolactic gene
DE3248145A1 (de) * 1982-12-27 1984-06-28 Boehringer Mannheim Gmbh, 6800 Mannheim Verfahren und reagenz zur bestimmung von n-carbamoylsarcosin und hierfuer geeignetes neues enzym
DE3406770A1 (de) * 1984-02-24 1985-08-29 Boehringer Mannheim Gmbh, 6800 Mannheim Nucleosidtriphosphat-abhaengige 1-methylhydantoinase und ihre verwendung
DE4000668A1 (de) * 1990-01-11 1991-07-18 Boehringer Mannheim Gmbh Verfahren und reagenz zur enzymatischen bestimmung von creatinin

Also Published As

Publication number Publication date
EP0476670A2 (de) 1992-03-25
EP0476670B1 (de) 1999-05-12
JP2627587B2 (ja) 1997-07-09
DE4029844A1 (de) 1992-03-26
US5416014A (en) 1995-05-16
DE59109126D1 (de) 1999-06-17
JPH0556788A (ja) 1993-03-09
ATE180015T1 (de) 1999-05-15
EP0476670A3 (en) 1992-10-07

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