ES2133236A1 - Gen quimerico formado por las secuencias de dna que codifican los determinantes antigenicos de cuatro proteinas de l. infantum, aplicable para el diagnostico serologico de leishmaniosis canina y proteina obtenida. - Google Patents

Gen quimerico formado por las secuencias de dna que codifican los determinantes antigenicos de cuatro proteinas de l. infantum, aplicable para el diagnostico serologico de leishmaniosis canina y proteina obtenida.

Info

Publication number
ES2133236A1
ES2133236A1 ES09701430A ES9701430A ES2133236A1 ES 2133236 A1 ES2133236 A1 ES 2133236A1 ES 09701430 A ES09701430 A ES 09701430A ES 9701430 A ES9701430 A ES 9701430A ES 2133236 A1 ES2133236 A1 ES 2133236A1
Authority
ES
Spain
Prior art keywords
proteins
code
chimaeric gene
infantum
dna sequences
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES09701430A
Other languages
English (en)
Other versions
ES2133236B1 (es
Inventor
Bedate Carlos Alonso
Rolania Jose Maria Requena
Alvarez Manuel Soto
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Laboratorios Leti SA
CBF Leti SA
Original Assignee
Laboratorios Leti SA
CBF Leti SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Laboratorios Leti SA, CBF Leti SA filed Critical Laboratorios Leti SA
Priority to ES009701430A priority Critical patent/ES2133236B1/es
Priority to CA2256125A priority patent/CA2256125C/en
Publication of ES2133236A1 publication Critical patent/ES2133236A1/es
Application granted granted Critical
Publication of ES2133236B1 publication Critical patent/ES2133236B1/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/44—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from protozoa
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Gen quimérico formado por las secuencias parciales de DNA que codifica los determinantes antigénicos de cuatro proteínas de Leishmania infantum (LiP2a, LiP2b, LiPH2A, LiP0) y la proteína obtenida, aplicable al diagnóstico de la Leishmaniasis. Se muestran las estrategias de clonaje de un gen quimérico y los productos intermedios generados durante el proceso de formación del gen. Se indica la utilización del clon parcial rLiPP0-Ct-Q como vector al cual, mediante el uso de enzimas de restricción apropiadas, se añaden secuencialmente en la dirección 5 los fragmentos parciales de DNA de las proteínas LiP2a, LiP2b y LiH2A. Se describen los pasos seguidos para la determinación de la orientación correcta de los insertos de DNA en el gen final y de los productos proteicos intermedios. El polipéptido final generado tiene una masa molecular de 38K y un punto isoeléctrico de 7.37. Se describe la secuencia de nucleótidos del gen quimérico y la secuencia deducida de aminoácidos de la proteína sintetizada.
ES009701430A 1997-06-27 1997-06-27 Gen quimerico formado por las secuencias de dna que codifican los determinantes antigenicos de cuatro proteinas de l. infantum, aplicable para el diagnostico serologico de leishmaniosis canina y proteina obtenida. Expired - Lifetime ES2133236B1 (es)

Priority Applications (2)

Application Number Priority Date Filing Date Title
ES009701430A ES2133236B1 (es) 1997-06-27 1997-06-27 Gen quimerico formado por las secuencias de dna que codifican los determinantes antigenicos de cuatro proteinas de l. infantum, aplicable para el diagnostico serologico de leishmaniosis canina y proteina obtenida.
CA2256125A CA2256125C (en) 1997-06-27 1998-12-23 Chimeric gene formed of the dna sequences that encode the antigenic determinants of four proteins of l. infantum, useful for serologic diagnosis of canine leishmaniasis and protein obtained

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ES009701430A ES2133236B1 (es) 1997-06-27 1997-06-27 Gen quimerico formado por las secuencias de dna que codifican los determinantes antigenicos de cuatro proteinas de l. infantum, aplicable para el diagnostico serologico de leishmaniosis canina y proteina obtenida.
CA2256125A CA2256125C (en) 1997-06-27 1998-12-23 Chimeric gene formed of the dna sequences that encode the antigenic determinants of four proteins of l. infantum, useful for serologic diagnosis of canine leishmaniasis and protein obtained

Publications (2)

Publication Number Publication Date
ES2133236A1 true ES2133236A1 (es) 1999-09-01
ES2133236B1 ES2133236B1 (es) 2000-04-16

Family

ID=32031338

Family Applications (1)

Application Number Title Priority Date Filing Date
ES009701430A Expired - Lifetime ES2133236B1 (es) 1997-06-27 1997-06-27 Gen quimerico formado por las secuencias de dna que codifican los determinantes antigenicos de cuatro proteinas de l. infantum, aplicable para el diagnostico serologico de leishmaniosis canina y proteina obtenida.

Country Status (2)

Country Link
CA (1) CA2256125C (es)
ES (1) ES2133236B1 (es)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000039298A1 (en) * 1998-12-23 2000-07-06 C.B.F. Leti S.A. Chimeric gene encoding the antigenic determinants of four proteins of l. infantum
WO2001081388A1 (es) 2000-04-17 2001-11-01 C.B.F. Leti, S.A. Composición que contiene leishmania lip2a
EP1624063A3 (en) * 1998-12-23 2006-07-05 C.B.F. Leti S.A. Chimeric gene encoding the antigenic determinants of four proteins of L. infantum

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2795149B2 (es) 2020-06-08 2022-07-04 Univ Madrid Complutense Quimera sintetica multiepitopica como vacuna y tratamiento frente a leishmaniosis en mamiferos

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996033414A2 (en) * 1995-04-21 1996-10-24 Corixa Corporation Compounds and methods for diagnosis of leishmaniasis

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996033414A2 (en) * 1995-04-21 1996-10-24 Corixa Corporation Compounds and methods for diagnosis of leishmaniasis

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
SOTO et al. "During active viscerocutaneous leishmaniasis the anti-P2 humoral response is specifically triggered by the parasite P proteins". 1995. Clin. Exp. Immnol. Vol. 100. Paginas 246-252. *
SOTO et al. "Genomic organization and expression of two independent gene arrays coding for two antigenic acidic ribosomal proteins of Leishmania". Octubre 1995. The journal of biological chemistry. Vol. 268. Paginas 21835-21843. *
SOTO et al. "Isolation, characterization and analysis of the expression of the Leishmania ribosomal PO protein genes". 1993. Molecular and Biochemical Parasitology. Vol. 61. Paginas 265-274. *
SOTO et al. "The nRNA coding for the nucleosomal protein H2A of Leishmania is polyadenylated and has stem-loops at the 3' end". 1991. Nucleic Acids Research. Vol. 19 (16). Pagina 4554. *
SOTO M. et al. "Identification of the Leishmania infatum PO ribosomal protein epitope in canine visceral leishmaniasis". 1995. Immunology Letters. Vol. 48. Paginas 23-28. *
SOTO M. et al. "Mapping of the linear antigenic determinants from the Leishmania infantum histone H2A recognized by sera from dogs with leishmaniasis". 1995. Immunology Letters. Vol. 48. Paginas 209-214. *
SOTO M. et al. "Specific serodiagnosis of Human Leishmaniasis with recombinant Leishmania P2 Acidic Ribosomal proteins". Julio 1996. Clinical and Diagnostic Laboratory Immunology. Vol. 3 (4). Paginas 387-391. *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000039298A1 (en) * 1998-12-23 2000-07-06 C.B.F. Leti S.A. Chimeric gene encoding the antigenic determinants of four proteins of l. infantum
EP1624063A3 (en) * 1998-12-23 2006-07-05 C.B.F. Leti S.A. Chimeric gene encoding the antigenic determinants of four proteins of L. infantum
WO2001081388A1 (es) 2000-04-17 2001-11-01 C.B.F. Leti, S.A. Composición que contiene leishmania lip2a
ES2169995A1 (es) * 2000-04-17 2002-07-16 Leti Lab Composicion a base de la proteina lip2a y su uso en la preparacion de un medicamento para suscitar una respuesta inmune contra un antigeno.

Also Published As

Publication number Publication date
ES2133236B1 (es) 2000-04-16
CA2256125C (en) 2012-06-19
CA2256125A1 (en) 2000-06-23

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