ES2125454T3 - Procedimiento para separar proteinas glicosiladas y no glicosiladas. - Google Patents

Procedimiento para separar proteinas glicosiladas y no glicosiladas.

Info

Publication number
ES2125454T3
ES2125454T3 ES94914953T ES94914953T ES2125454T3 ES 2125454 T3 ES2125454 T3 ES 2125454T3 ES 94914953 T ES94914953 T ES 94914953T ES 94914953 T ES94914953 T ES 94914953T ES 2125454 T3 ES2125454 T3 ES 2125454T3
Authority
ES
Spain
Prior art keywords
prolactin
glycosylated
eluting
organic solvent
glycosilated
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES94914953T
Other languages
English (en)
Inventor
Albert E Price
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genzyme Corp
Original Assignee
Genzyme Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genzyme Corp filed Critical Genzyme Corp
Application granted granted Critical
Publication of ES2125454T3 publication Critical patent/ES2125454T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/57554—Prolactin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Endocrinology (AREA)
  • Peptides Or Proteins (AREA)
  • Saccharide Compounds (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

LA INVENCION SE REFIERE A UN METODO PARA SEPARAR PROTEINAS GLIEUSILADAS Y NO GLIEUSILADAS. EL METODO COMPRENDE LAS SIGUIENTES FASES: 1) EQUILIBRAR LA SOLUCION QUE CONTIENE LA PROTEINA CON UNA SOLUCION EQUILIBRANTE QUE CONTIENE AL MENOS UN SOLVENTE ORGANICO; 2) AÑADIR LA SOLUCION DE PROTEINA EQUILIBRADA A UNA COLUMNA CROMATAGRAFICA QUE CONTIENE UN EMPAQUETADO PARA LA SEPARACION POR AFINIDAD DEL INTERCAMBIO DE IONES DE LAS PROTEINAS GLIEUSILADAS Y NO GLIEUSILADAS; 3) ELUIR LAS PROTEINAS GLIEUSILADAS DE LA COLUMNA CROMATOGRAFICA CON UNA CANTIDAD SUFICIENTE DE UNA PRIMERA SOLUCION DE ELUCION, QUE CONTIENE AL MENOS UN SOLVENTE ORGANICO Y CON O SIN AL MENOS UNA SAL, DONDE LA CONCENTRACION DE LA SAL Y/O LA CONCENTRACION DEL SOLVENTE ORGANICO Y/O EL PH SON APROPIADOS PARA ELUIR LAS PROTEINAS GLIEUSILADAS QUE ESTEN SUSTANCIALMENTE LIBRES DE PROTEINAS NO GLIEUSILADAS; Y 4) ELUIR LAS PROTEINAS NO GLUCOSILADAS DE LA COLUMNA CROMATOGRAFICA CON UNA CANTIDAD SUFICIENTE DE UNA SEGUNDA SOLUCION DE ELUCION QUE CONTENGA AL MENOS UN SOLVENTE ORGANICO, Y CON O SIN AL MENOS UNA SAL, DONDE LA CONCENTRACION DE SAL Y/O LA CONCENTRACION DE SOLVENTE ORGANICO Y/O EL PH SEAN APROPIADOS PARA ELUIR LAS PROTEINAS NO GLIEUSILADAS QUE ESTEN SUSTANCIALMENTE LIBRES DE PROTEINAS GLIEUSILADAS.
ES94914953T 1993-05-04 1994-05-04 Procedimiento para separar proteinas glicosiladas y no glicosiladas. Expired - Lifetime ES2125454T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/057,587 US5344920A (en) 1993-05-04 1993-05-04 Method of separating glycosylated and non-glycosylated prolactin by ion exchange chromatography

Publications (1)

Publication Number Publication Date
ES2125454T3 true ES2125454T3 (es) 1999-03-01

Family

ID=22011527

Family Applications (1)

Application Number Title Priority Date Filing Date
ES94914953T Expired - Lifetime ES2125454T3 (es) 1993-05-04 1994-05-04 Procedimiento para separar proteinas glicosiladas y no glicosiladas.

Country Status (10)

Country Link
US (1) US5344920A (es)
EP (1) EP0698038B1 (es)
JP (1) JPH08509743A (es)
AT (1) ATE175214T1 (es)
AU (1) AU674414B2 (es)
CA (1) CA2160703A1 (es)
DE (1) DE69415664T2 (es)
DK (1) DK0698038T3 (es)
ES (1) ES2125454T3 (es)
WO (1) WO1994025481A1 (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6210736B1 (en) * 1997-06-17 2001-04-03 Genzyme Transgenics Corporation Transgenically produced prolactin
US20060011547A1 (en) * 2004-07-13 2006-01-19 Bell Stephen A Methods of separating components in treatment fluids

Also Published As

Publication number Publication date
EP0698038A1 (en) 1996-02-28
EP0698038B1 (en) 1998-12-30
JPH08509743A (ja) 1996-10-15
WO1994025481A1 (en) 1994-11-10
CA2160703A1 (en) 1994-11-10
DK0698038T3 (da) 1999-08-30
DE69415664T2 (de) 1999-07-15
AU674414B2 (en) 1996-12-19
AU6716594A (en) 1994-11-21
ATE175214T1 (de) 1999-01-15
US5344920A (en) 1994-09-06
DE69415664D1 (de) 1999-02-11

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