ES2125175A1 - Ribosome-inactivator protein, by the name of basic nigrin b, from the plant Sambucus nigra L., process for obtaining and preparing it - Google Patents
Ribosome-inactivator protein, by the name of basic nigrin b, from the plant Sambucus nigra L., process for obtaining and preparing itInfo
- Publication number
- ES2125175A1 ES2125175A1 ES09602245A ES9602245A ES2125175A1 ES 2125175 A1 ES2125175 A1 ES 2125175A1 ES 09602245 A ES09602245 A ES 09602245A ES 9602245 A ES9602245 A ES 9602245A ES 2125175 A1 ES2125175 A1 ES 2125175A1
- Authority
- ES
- Spain
- Prior art keywords
- inactivator
- obtaining
- sambucus nigra
- ribosome
- plant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
Ribosome-inactivator protein, by the name of basic nigrin b, from the plant Sambucus nigra L., process for obtaining and preparing it. Basic nigrin b has a relative molecular mass determined by polyacrylamide gel electrophoresis of 64000 in the absence of reducing agent and 32000 for the B chain, and 32000 for the A chain in the presence of reducing agent. The general process for obtaining RIPs from Sambucus nigra according to this invention comprises first extraction operations from the bark of the plant with an aqueous solution based on sodium chloride and monosodium phosphate in order to obtain an extract which can inhibit protein synthesis, and purification of the same through ion exchange and molecular exclusion chromatography techniques. Basic nigrin b is used as a ribonucleic acid inactivator and an inhibitor of the biosynthesis of proteins.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES9602245A ES2125175B1 (en) | 1996-10-07 | 1996-10-07 | RIBOSOME INACTIVATING PROTEIN, CALLED NIGRINE B BASIC, FROM THE SAMBUCUS NIGRA L. PLANT, PROCEDURE FOR ITS OBTAINING AND USE. |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES9602245A ES2125175B1 (en) | 1996-10-07 | 1996-10-07 | RIBOSOME INACTIVATING PROTEIN, CALLED NIGRINE B BASIC, FROM THE SAMBUCUS NIGRA L. PLANT, PROCEDURE FOR ITS OBTAINING AND USE. |
Publications (2)
Publication Number | Publication Date |
---|---|
ES2125175A1 true ES2125175A1 (en) | 1999-02-16 |
ES2125175B1 ES2125175B1 (en) | 1999-10-16 |
Family
ID=8296469
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES9602245A Expired - Fee Related ES2125175B1 (en) | 1996-10-07 | 1996-10-07 | RIBOSOME INACTIVATING PROTEIN, CALLED NIGRINE B BASIC, FROM THE SAMBUCUS NIGRA L. PLANT, PROCEDURE FOR ITS OBTAINING AND USE. |
Country Status (1)
Country | Link |
---|---|
ES (1) | ES2125175B1 (en) |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1994020540A1 (en) * | 1993-03-02 | 1994-09-15 | Universidad De Valladolid | NON-TOXIC RIBOSOME INACTIVATING PROTEINS (RIPs) WITH TWO CHAINS, PROCESS FOR THE PREPARATION THEREOF AND APPLICATIONS |
-
1996
- 1996-10-07 ES ES9602245A patent/ES2125175B1/en not_active Expired - Fee Related
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1994020540A1 (en) * | 1993-03-02 | 1994-09-15 | Universidad De Valladolid | NON-TOXIC RIBOSOME INACTIVATING PROTEINS (RIPs) WITH TWO CHAINS, PROCESS FOR THE PREPARATION THEREOF AND APPLICATIONS |
Also Published As
Publication number | Publication date |
---|---|
ES2125175B1 (en) | 1999-10-16 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EC2A | Search report published |
Date of ref document: 19990216 Kind code of ref document: A1 Effective date: 19990216 |
|
FD2A | Announcement of lapse in spain |
Effective date: 20170126 |