ES2125175A1 - Ribosome-inactivator protein, by the name of basic nigrin b, from the plant Sambucus nigra L., process for obtaining and preparing it - Google Patents

Ribosome-inactivator protein, by the name of basic nigrin b, from the plant Sambucus nigra L., process for obtaining and preparing it

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Publication number
ES2125175A1
ES2125175A1 ES09602245A ES9602245A ES2125175A1 ES 2125175 A1 ES2125175 A1 ES 2125175A1 ES 09602245 A ES09602245 A ES 09602245A ES 9602245 A ES9602245 A ES 9602245A ES 2125175 A1 ES2125175 A1 ES 2125175A1
Authority
ES
Spain
Prior art keywords
inactivator
obtaining
sambucus nigra
ribosome
plant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES09602245A
Other languages
Spanish (es)
Other versions
ES2125175B1 (en
Inventor
Juan Tomas Girbes
De Benito Fernando Martinez
Alvarez Rosario Iglesias
Gonzalez Lucia Citores
Rodriguez Jose Miguel Ferreras
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universidad de Valladolid
Original Assignee
Universidad de Valladolid
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universidad de Valladolid filed Critical Universidad de Valladolid
Priority to ES9602245A priority Critical patent/ES2125175B1/en
Publication of ES2125175A1 publication Critical patent/ES2125175A1/en
Application granted granted Critical
Publication of ES2125175B1 publication Critical patent/ES2125175B1/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Ribosome-inactivator protein, by the name of basic nigrin b, from the plant Sambucus nigra L., process for obtaining and preparing it. Basic nigrin b has a relative molecular mass determined by polyacrylamide gel electrophoresis of 64000 in the absence of reducing agent and 32000 for the B chain, and 32000 for the A chain in the presence of reducing agent. The general process for obtaining RIPs from Sambucus nigra according to this invention comprises first extraction operations from the bark of the plant with an aqueous solution based on sodium chloride and monosodium phosphate in order to obtain an extract which can inhibit protein synthesis, and purification of the same through ion exchange and molecular exclusion chromatography techniques. Basic nigrin b is used as a ribonucleic acid inactivator and an inhibitor of the biosynthesis of proteins.
ES9602245A 1996-10-07 1996-10-07 RIBOSOME INACTIVATING PROTEIN, CALLED NIGRINE B BASIC, FROM THE SAMBUCUS NIGRA L. PLANT, PROCEDURE FOR ITS OBTAINING AND USE. Expired - Fee Related ES2125175B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES9602245A ES2125175B1 (en) 1996-10-07 1996-10-07 RIBOSOME INACTIVATING PROTEIN, CALLED NIGRINE B BASIC, FROM THE SAMBUCUS NIGRA L. PLANT, PROCEDURE FOR ITS OBTAINING AND USE.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES9602245A ES2125175B1 (en) 1996-10-07 1996-10-07 RIBOSOME INACTIVATING PROTEIN, CALLED NIGRINE B BASIC, FROM THE SAMBUCUS NIGRA L. PLANT, PROCEDURE FOR ITS OBTAINING AND USE.

Publications (2)

Publication Number Publication Date
ES2125175A1 true ES2125175A1 (en) 1999-02-16
ES2125175B1 ES2125175B1 (en) 1999-10-16

Family

ID=8296469

Family Applications (1)

Application Number Title Priority Date Filing Date
ES9602245A Expired - Fee Related ES2125175B1 (en) 1996-10-07 1996-10-07 RIBOSOME INACTIVATING PROTEIN, CALLED NIGRINE B BASIC, FROM THE SAMBUCUS NIGRA L. PLANT, PROCEDURE FOR ITS OBTAINING AND USE.

Country Status (1)

Country Link
ES (1) ES2125175B1 (en)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994020540A1 (en) * 1993-03-02 1994-09-15 Universidad De Valladolid NON-TOXIC RIBOSOME INACTIVATING PROTEINS (RIPs) WITH TWO CHAINS, PROCESS FOR THE PREPARATION THEREOF AND APPLICATIONS

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994020540A1 (en) * 1993-03-02 1994-09-15 Universidad De Valladolid NON-TOXIC RIBOSOME INACTIVATING PROTEINS (RIPs) WITH TWO CHAINS, PROCESS FOR THE PREPARATION THEREOF AND APPLICATIONS

Also Published As

Publication number Publication date
ES2125175B1 (en) 1999-10-16

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