ES2095190A1 - Ribosome inactivator proteins known as Alpha-nigritin, Beta-nigritin and Gamma-nigritin, from the plant Sambucus nigra L., process for obtaining them and their use - Google Patents

Ribosome inactivator proteins known as Alpha-nigritin, Beta-nigritin and Gamma-nigritin, from the plant Sambucus nigra L., process for obtaining them and their use

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Publication number
ES2095190A1
ES2095190A1 ES09500509A ES9500509A ES2095190A1 ES 2095190 A1 ES2095190 A1 ES 2095190A1 ES 09500509 A ES09500509 A ES 09500509A ES 9500509 A ES9500509 A ES 9500509A ES 2095190 A1 ES2095190 A1 ES 2095190A1
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Spain
Prior art keywords
nigritin
ser
gamma
alpha
ile
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Granted
Application number
ES09500509A
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Spanish (es)
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ES2095190B1 (en
Inventor
Juan Tomas Girbes
De Benito Fernando Martinez
Gonzalez Lucia Citores
Alvarez Rosario Iglesias
Rodriguez Jose Miguel Ferreras
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Universidad de Valladolid
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Universidad de Valladolid
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Priority to ES9500509A priority Critical patent/ES2095190B1/en
Publication of ES2095190A1 publication Critical patent/ES2095190A1/en
Application granted granted Critical
Publication of ES2095190B1 publication Critical patent/ES2095190B1/en
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Expired - Fee Related legal-status Critical Current

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Abstract

Ribosome inactivator proteins known as Alpha-nigritin, Beta-nigritin and Gamma-nigritin, from the plant Sambucus nigra L., process for obtaining them and their use Alpha-nigritin, Beta-nigritin and Gamma-nigritin have relative molecular masses of 29,000, 40,000 and 27,500 daltons respectively. Alpha-nigritin has the following amino acid sequence at its amine terminal end: Ala-Ser-Val-Leu-Asp-Ser-Ile-Gln-Phe-Val-Ser-Leu-Glu-Lys- Xxx-Ala-Val-Tyr-Glu-Gln-Asn. Gamma-nigritin has three tryptic peptides with the following amino acid sequences: a) Val-Thr-Asn-Ser-Phe-Tyr-Leu b) Tyr-Xxx-Pro-Ser-Ile-Ile-Arg c) Trp-Xxx-Asn-Thr-His-Ser-Tyr-Leu-Asn-Glu-Ile-Phe-Ile- Xxx-Leu. The general process for obtaining RIPs from Sambucus nigra according to this invention comprises isolating these from the leaves of Sambucus nigra including a first extraction operation with an aqueous solution based on sodium chloride and monosodium phosphate to obtain an extract which is capable of inhibiting protein synthesis, followed by purification of the extract using ion exchange and molecular exclusion chromatography techniques. Alpha-nigritin, Beta-nigritin and Gamma-nigritin are used as ribonucleic acid inactivators and as inhibitors of protein biosynthesis.
ES9500509A 1995-03-06 1995-03-06 RIBOSOME INACTIVATING PROTEINS, CALLED ALPHA-NIGRITINE, BETA-NIGRITINE AND GAMMA-NIGRITINE, FROM THE SAMBUCUS NIGRA L. PLANT, PROCEDURE FOR THEIR OBTAINING AND USE. Expired - Fee Related ES2095190B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES9500509A ES2095190B1 (en) 1995-03-06 1995-03-06 RIBOSOME INACTIVATING PROTEINS, CALLED ALPHA-NIGRITINE, BETA-NIGRITINE AND GAMMA-NIGRITINE, FROM THE SAMBUCUS NIGRA L. PLANT, PROCEDURE FOR THEIR OBTAINING AND USE.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES9500509A ES2095190B1 (en) 1995-03-06 1995-03-06 RIBOSOME INACTIVATING PROTEINS, CALLED ALPHA-NIGRITINE, BETA-NIGRITINE AND GAMMA-NIGRITINE, FROM THE SAMBUCUS NIGRA L. PLANT, PROCEDURE FOR THEIR OBTAINING AND USE.

Publications (2)

Publication Number Publication Date
ES2095190A1 true ES2095190A1 (en) 1997-02-01
ES2095190B1 ES2095190B1 (en) 1997-09-01

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ES9500509A Expired - Fee Related ES2095190B1 (en) 1995-03-06 1995-03-06 RIBOSOME INACTIVATING PROTEINS, CALLED ALPHA-NIGRITINE, BETA-NIGRITINE AND GAMMA-NIGRITINE, FROM THE SAMBUCUS NIGRA L. PLANT, PROCEDURE FOR THEIR OBTAINING AND USE.

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Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994020540A1 (en) * 1993-03-02 1994-09-15 Universidad De Valladolid NON-TOXIC RIBOSOME INACTIVATING PROTEINS (RIPs) WITH TWO CHAINS, PROCESS FOR THE PREPARATION THEREOF AND APPLICATIONS

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994020540A1 (en) * 1993-03-02 1994-09-15 Universidad De Valladolid NON-TOXIC RIBOSOME INACTIVATING PROTEINS (RIPs) WITH TWO CHAINS, PROCESS FOR THE PREPARATION THEREOF AND APPLICATIONS

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
GIRBES, T. et al. "Isolation and partial characterization of nigrin b, a non-toxic novel type 2 ribosome-inactivating protein from the bark of Sambucus nigra L." PLANT MOLECULAR BIOLOGY. 1993. Vol. 22, pßginas 1181-1186 * Todo el documento * *

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Publication number Publication date
ES2095190B1 (en) 1997-09-01

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