ES2085359T3 - Columnas rellenas de gel apropiadas para usarlas en cromatografia. - Google Patents

Columnas rellenas de gel apropiadas para usarlas en cromatografia.

Info

Publication number
ES2085359T3
ES2085359T3 ES90915583T ES90915583T ES2085359T3 ES 2085359 T3 ES2085359 T3 ES 2085359T3 ES 90915583 T ES90915583 T ES 90915583T ES 90915583 T ES90915583 T ES 90915583T ES 2085359 T3 ES2085359 T3 ES 2085359T3
Authority
ES
Spain
Prior art keywords
gel
capillary
chromatography
columns filled
gel suitable
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES90915583T
Other languages
English (en)
Inventor
Robert S Dubrow
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Applied Biosystems Inc
Original Assignee
Perkin Elmer Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Perkin Elmer Corp filed Critical Perkin Elmer Corp
Application granted granted Critical
Publication of ES2085359T3 publication Critical patent/ES2085359T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/50—Conditioning of the sorbent material or stationary liquid
    • G01N30/56—Packing methods or coating methods

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)

Abstract

UN METODO PARA LLENAR UN CAPILAR (15) CON UN GEL SUSTANCIALMENTE SIN VACIO, FORMANDO EL GEL FUERA DEL CAPILAR (15) Y DESPUES EXTRUSIONAR EL GEL EN EL CAPILAR (15). EL GEL INICIAL SE PUEDE FORMAR COMO UNA VARILLA DE GEL CON DIAMETRO MUCHO MAS GRANDE QUE EL CAPILAR (15) EN EL QUE ES EXTRUIDO. ESTA VARILLA DE GEL ES ACLARADO PARA EXTRAER LOS CONTAMINANTES DE POLIMERIZACION Y DESPUES ES SECADA PARA FORMAR UNA PREFORMA DE GEL SECA. EL GEL SE PUEDE DESCOMPONER CON UN SOLVENTE COMPATIBLE Y DESPUES EXTRUIR EN UNO O MAS CAPILARES.
ES90915583T 1989-10-03 1990-10-02 Columnas rellenas de gel apropiadas para usarlas en cromatografia. Expired - Lifetime ES2085359T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/416,588 US5427729A (en) 1989-10-03 1989-10-03 Method of making gel packed columns suitable for use in chromatography

Publications (1)

Publication Number Publication Date
ES2085359T3 true ES2085359T3 (es) 1996-06-01

Family

ID=23650552

Family Applications (1)

Application Number Title Priority Date Filing Date
ES90915583T Expired - Lifetime ES2085359T3 (es) 1989-10-03 1990-10-02 Columnas rellenas de gel apropiadas para usarlas en cromatografia.

Country Status (7)

Country Link
US (1) US5427729A (es)
EP (1) EP0494958B1 (es)
AT (1) ATE134768T1 (es)
DE (1) DE69025620T2 (es)
DK (1) DK0494958T3 (es)
ES (1) ES2085359T3 (es)
WO (1) WO1991005084A1 (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5332481A (en) * 1991-01-29 1994-07-26 Beckman Instruments, Inc. Capillary electrophoresis using replaceable gels
US5756454A (en) * 1991-09-05 1998-05-26 Schering Aktiengesellschaft Collagen-induced platelet aggregation inhibitor
US5635050A (en) * 1995-08-23 1997-06-03 Beckman Instruments, Inc. Electrophoretic system including means for replacing separation medium
JP2000162183A (ja) * 1998-11-30 2000-06-16 Inst Of Physical & Chemical Res キャピラリー電気泳動装置
WO2005047881A2 (en) * 2003-11-05 2005-05-26 Exact Sciences Corporation Repetitive reversed-field affinity electrophoresis and uses therefor
TWI248863B (en) * 2004-02-12 2006-02-11 Mitsubishi Heavy Ind Ltd Apparatus and method for mold temperature adjustment, and mold temperature control unit
US9063082B2 (en) * 2009-11-27 2015-06-23 Ge Healthcare Bio-Sciences Ab Printing of electrophoresis gels
WO2017040981A1 (en) * 2015-09-03 2017-03-09 University Of Florida Research Foundation, Inc. Valve incorporating temporary phase change material

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3796657A (en) * 1965-05-11 1974-03-12 V Pretorius Apparatus for distribution separation processes,their manufacture and use
US4208284A (en) * 1965-05-11 1980-06-17 Hahn Hans H Apparatus for distribution separation processes
US3523863A (en) * 1966-11-09 1970-08-11 Beckman Instruments Inc Drying and preservation of electrophoresis gel films
US3527712A (en) * 1967-03-07 1970-09-08 Marine Colloids Inc Dried agarose gel,method of preparation thereof,and production of aqueous agarose gel
GB1223589A (en) * 1967-12-18 1971-02-24 South African Inventions Improvements relating to a medium for separations
US3875044A (en) * 1972-06-09 1975-04-01 Marine Colloids Inc Hydratable gel sheets
US4189370A (en) * 1978-06-21 1980-02-19 Societe Sebia Process for obtaining gels of N-methylol-acrylamide copolymers and application of said gels for the stepped gradient separation of seric lipoproteins
US4279361A (en) * 1979-07-16 1981-07-21 Chung Thomas Y Gel extruding method and apparatus
FR2533836B1 (fr) * 1982-10-05 1988-01-22 Elf Aquitaine Procede et dispositif de remplissage de colonnes chromatographiques
JPS6060549A (ja) * 1983-09-14 1985-04-08 Fuji Photo Film Co Ltd ゲル電気泳動用媒体
US4597421A (en) * 1984-11-19 1986-07-01 Varian Associates, Inc. Method and device for on-column injection of a liquid sample into small diameter columns
US4675300A (en) * 1985-09-18 1987-06-23 The Board Of Trustees Of The Leland Stanford Junior University Laser-excitation fluorescence detection electrokinetic separation
US4865706A (en) * 1986-10-21 1989-09-12 Northeastern University High performance microcapillary gel electrophoresis
JPS63298046A (ja) * 1987-05-29 1988-12-05 Fuji Photo Film Co Ltd 電気泳動用グラジェントゲル膜の製造方法

Also Published As

Publication number Publication date
DE69025620T2 (de) 1996-09-19
EP0494958B1 (en) 1996-02-28
DK0494958T3 (da) 1996-07-08
EP0494958A4 (en) 1993-02-17
EP0494958A1 (en) 1992-07-22
WO1991005084A1 (en) 1991-04-18
ATE134768T1 (de) 1996-03-15
US5427729A (en) 1995-06-27
DE69025620D1 (de) 1996-04-04

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