ES2056798T3 - Procedimiento para la obtencion de proteinas por tecnologia genetica con utilizacion de celulas hospedantes gram-negativas. - Google Patents

Procedimiento para la obtencion de proteinas por tecnologia genetica con utilizacion de celulas hospedantes gram-negativas.

Info

Publication number
ES2056798T3
ES2056798T3 ES87109492T ES87109492T ES2056798T3 ES 2056798 T3 ES2056798 T3 ES 2056798T3 ES 87109492 T ES87109492 T ES 87109492T ES 87109492 T ES87109492 T ES 87109492T ES 2056798 T3 ES2056798 T3 ES 2056798T3
Authority
ES
Spain
Prior art keywords
gene
proteins
pct
gram
obtaining
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES87109492T
Other languages
English (en)
Inventor
Thomas Ferdinand Dr Meyer
Roman Halter
Johannes Pohlner
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Max Planck Gesellschaft zur Foerderung der Wissenschaften eV
Original Assignee
Max Planck Gesellschaft zur Foerderung der Wissenschaften eV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Max Planck Gesellschaft zur Foerderung der Wissenschaften eV filed Critical Max Planck Gesellschaft zur Foerderung der Wissenschaften eV
Application granted granted Critical
Publication of ES2056798T3 publication Critical patent/ES2056798T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

PARA LA OBTENCION DE PROTEINAS POR INGENIERIA GENETICA SE INTRODUCE EN CELULAS HUESPED GRAM NEGATIVAS, UN VECTOR QUE CONTENGA POR LO MENOS UN GEN CODIFICADOR DE LA PROTEINA DESEADA Y EL GEN SE TRANSCRIBE Y SE TRADUCE. PARA LA OBTENCION EXTRACELULAR DE LA PROTEINA SE INCORPORA SU GEN CODIFICADOR EN UN VECTOR, EL GEN PRECURSOR DE IGA-PROTEASA DE MICROORGANISMOS DEL GENERO NEISSERIA, DE MODO QUE EL GEN CODIFICADOR ESTE SITUADO DENTRO DE LA SECUENCIA DEL GEN PRECURSOR DE IGA-PROTEASA. UN PLASMIDO ESPECIALMENTE ADECUADO PARA ELLO ES EL PLASMIDO PLP100 CONTENIDO EN E.COLI DSM 3775.
ES87109492T 1986-07-02 1987-07-01 Procedimiento para la obtencion de proteinas por tecnologia genetica con utilizacion de celulas hospedantes gram-negativas. Expired - Lifetime ES2056798T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19863622221 DE3622221A1 (de) 1986-07-02 1986-07-02 Verfahren zur gentechnologischen gewinnung von proteinen unter verwendung gramnegativer wirtszellen

Publications (1)

Publication Number Publication Date
ES2056798T3 true ES2056798T3 (es) 1994-10-16

Family

ID=6304241

Family Applications (1)

Application Number Title Priority Date Filing Date
ES87109492T Expired - Lifetime ES2056798T3 (es) 1986-07-02 1987-07-01 Procedimiento para la obtencion de proteinas por tecnologia genetica con utilizacion de celulas hospedantes gram-negativas.

Country Status (7)

Country Link
US (1) US5268270A (es)
EP (1) EP0254090B1 (es)
JP (1) JPH07100039B2 (es)
AT (1) ATE89318T1 (es)
DE (2) DE3622221A1 (es)
ES (1) ES2056798T3 (es)
WO (1) WO1988000238A1 (es)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4039415A1 (de) 1990-02-03 1991-08-08 Boehringer Mannheim Gmbh Verfahren zur herstellung rekombinanter proteine ohne n-terminalen methioninrest
NZ236819A (en) * 1990-02-03 1993-07-27 Max Planck Gesellschaft Enzymatic cleavage of fusion proteins; fusion proteins; recombinant dna and pharmaceutical compositions
DE4140699A1 (de) * 1991-01-11 1992-07-16 Boehringer Mannheim Gmbh Rekombinante iga-protease
US5965424A (en) * 1991-01-11 1999-10-12 Boehringer Mannheim Gmbh Methods for making neisseria or hemophilus IgA protease and DNA encoding the proteases
AU3651595A (en) * 1994-09-21 1996-04-09 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. Drug for the prevention and treatment of auto-immune and viral diseases, and diagnostic agents for detecting said diseases
GB9618960D0 (en) * 1996-09-11 1996-10-23 Medical Science Sys Inc Proteases
JP2000507274A (ja) * 1997-01-21 2000-06-13 マックス―プランク―ゲゼルシャフト・ツア・フェルデルング・デア・ヴィッセンシャフテン・アインゲトラーゲナー・フェアアイン・ベルリン キャリヤーペプチドとしてのｉｇａ１プロテアーゼフラグメント
GB0121591D0 (en) 2001-09-06 2001-10-24 Chiron Spa Hybrid and tandem expression of neisserial proteins
JP2006500963A (ja) * 2002-08-02 2006-01-12 グラクソスミスクライン バイオロジカルズ ソシエテ アノニム ワクチン組成物
AU2003274511B2 (en) * 2002-10-11 2009-06-04 Glaxosmithkline Biologicals S.A. Polypeptide-vaccines for broad protection against hypervirulent meningococcal lineages
EP2338987A1 (en) 2009-11-16 2011-06-29 ZYRUS Beteiligungsgesellschaft mbH & Co. Patente I KG Whole cell biocatalyst
WO2012038508A1 (en) 2010-09-23 2012-03-29 Autodisplay Biotech Gmbh Surface display of polypeptides containing a metal porphyrin or a flavin
WO2012152923A1 (en) 2011-05-12 2012-11-15 Autodisplay Biotech Gmbh Whole cell biocatalyst comprising a prenyltransferase
EP2970953B1 (en) 2013-03-13 2020-01-15 Autodisplay Biotech GmbH Improved surface display of functional proteins in a broad range of gram negative bacteria
WO2017186784A1 (en) 2016-04-26 2017-11-02 Autodisplay Biotech Gmbh Autodisplay of antibodies

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4338397A (en) * 1980-04-11 1982-07-06 President And Fellows Of Harvard College Mature protein synthesis

Also Published As

Publication number Publication date
JPH07100039B2 (ja) 1995-11-01
EP0254090B1 (de) 1993-05-12
DE3622221A1 (de) 1988-01-14
ATE89318T1 (de) 1993-05-15
US5268270A (en) 1993-12-07
JPH01500164A (ja) 1989-01-26
EP0254090A1 (de) 1988-01-27
DE3785798D1 (de) 1993-06-17
WO1988000238A1 (en) 1988-01-14

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