ES2056798T3 - Procedimiento para la obtencion de proteinas por tecnologia genetica con utilizacion de celulas hospedantes gram-negativas. - Google Patents
Procedimiento para la obtencion de proteinas por tecnologia genetica con utilizacion de celulas hospedantes gram-negativas.Info
- Publication number
- ES2056798T3 ES2056798T3 ES87109492T ES87109492T ES2056798T3 ES 2056798 T3 ES2056798 T3 ES 2056798T3 ES 87109492 T ES87109492 T ES 87109492T ES 87109492 T ES87109492 T ES 87109492T ES 2056798 T3 ES2056798 T3 ES 2056798T3
- Authority
- ES
- Spain
- Prior art keywords
- gene
- proteins
- pct
- gram
- obtaining
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
- C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/50—Fusion polypeptide containing protease site
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
Abstract
PARA LA OBTENCION DE PROTEINAS POR INGENIERIA GENETICA SE INTRODUCE EN CELULAS HUESPED GRAM NEGATIVAS, UN VECTOR QUE CONTENGA POR LO MENOS UN GEN CODIFICADOR DE LA PROTEINA DESEADA Y EL GEN SE TRANSCRIBE Y SE TRADUCE. PARA LA OBTENCION EXTRACELULAR DE LA PROTEINA SE INCORPORA SU GEN CODIFICADOR EN UN VECTOR, EL GEN PRECURSOR DE IGA-PROTEASA DE MICROORGANISMOS DEL GENERO NEISSERIA, DE MODO QUE EL GEN CODIFICADOR ESTE SITUADO DENTRO DE LA SECUENCIA DEL GEN PRECURSOR DE IGA-PROTEASA. UN PLASMIDO ESPECIALMENTE ADECUADO PARA ELLO ES EL PLASMIDO PLP100 CONTENIDO EN E.COLI DSM 3775.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE19863622221 DE3622221A1 (de) | 1986-07-02 | 1986-07-02 | Verfahren zur gentechnologischen gewinnung von proteinen unter verwendung gramnegativer wirtszellen |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2056798T3 true ES2056798T3 (es) | 1994-10-16 |
Family
ID=6304241
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES87109492T Expired - Lifetime ES2056798T3 (es) | 1986-07-02 | 1987-07-01 | Procedimiento para la obtencion de proteinas por tecnologia genetica con utilizacion de celulas hospedantes gram-negativas. |
Country Status (7)
Country | Link |
---|---|
US (1) | US5268270A (es) |
EP (1) | EP0254090B1 (es) |
JP (1) | JPH07100039B2 (es) |
AT (1) | ATE89318T1 (es) |
DE (2) | DE3622221A1 (es) |
ES (1) | ES2056798T3 (es) |
WO (1) | WO1988000238A1 (es) |
Families Citing this family (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE4039415A1 (de) | 1990-02-03 | 1991-08-08 | Boehringer Mannheim Gmbh | Verfahren zur herstellung rekombinanter proteine ohne n-terminalen methioninrest |
NZ236819A (en) * | 1990-02-03 | 1993-07-27 | Max Planck Gesellschaft | Enzymatic cleavage of fusion proteins; fusion proteins; recombinant dna and pharmaceutical compositions |
DE4140699A1 (de) * | 1991-01-11 | 1992-07-16 | Boehringer Mannheim Gmbh | Rekombinante iga-protease |
US5965424A (en) * | 1991-01-11 | 1999-10-12 | Boehringer Mannheim Gmbh | Methods for making neisseria or hemophilus IgA protease and DNA encoding the proteases |
WO1996009395A2 (de) * | 1994-09-21 | 1996-03-28 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | Arzneimittel zur prophylaxe und behandlung von und diagnostika zur erkennung von autoimmunkrankheiten und viralen erkrankungen |
GB9618960D0 (en) * | 1996-09-11 | 1996-10-23 | Medical Science Sys Inc | Proteases |
AU6211698A (en) * | 1997-01-21 | 1998-08-07 | Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. | Iga1 protease fragment as carrier peptide |
GB0121591D0 (en) | 2001-09-06 | 2001-10-24 | Chiron Spa | Hybrid and tandem expression of neisserial proteins |
SI2255826T1 (sl) * | 2002-08-02 | 2016-07-29 | Glaxosmithkline Biologicals S.A. | Neisserijski vakcinski sestavki, ki obsegajo kombinacijo antigenov |
SI1549338T1 (sl) * | 2002-10-11 | 2011-04-29 | Novartis Vaccines & Diagnostic | Polipeptidna cepiva za ĺ iroko zaĺ äśito pred hipervirulentnimi miningokoknimi rodovi |
EP2338987A1 (en) | 2009-11-16 | 2011-06-29 | ZYRUS Beteiligungsgesellschaft mbH & Co. Patente I KG | Whole cell biocatalyst |
EP2619306B1 (en) | 2010-09-23 | 2015-05-20 | Autodisplay Biotech GmbH | Surface display of polypeptides containing a metal porphyrin or a flavin |
WO2012152923A1 (en) | 2011-05-12 | 2012-11-15 | Autodisplay Biotech Gmbh | Whole cell biocatalyst comprising a prenyltransferase |
CN105164260B (zh) | 2013-03-13 | 2019-05-31 | 自动显示生物技术有限公司 | 功能性蛋白在范围广泛的革兰氏阴性细菌中的改善的表面展示 |
WO2017186784A1 (en) | 2016-04-26 | 2017-11-02 | Autodisplay Biotech Gmbh | Autodisplay of antibodies |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4338397A (en) * | 1980-04-11 | 1982-07-06 | President And Fellows Of Harvard College | Mature protein synthesis |
-
1986
- 1986-07-02 DE DE19863622221 patent/DE3622221A1/de not_active Withdrawn
-
1987
- 1987-07-01 EP EP87109492A patent/EP0254090B1/de not_active Expired - Lifetime
- 1987-07-01 AT AT87109492T patent/ATE89318T1/de not_active IP Right Cessation
- 1987-07-01 JP JP62504234A patent/JPH07100039B2/ja not_active Expired - Lifetime
- 1987-07-01 US US07/171,872 patent/US5268270A/en not_active Expired - Fee Related
- 1987-07-01 DE DE8787109492T patent/DE3785798D1/de not_active Expired - Fee Related
- 1987-07-01 ES ES87109492T patent/ES2056798T3/es not_active Expired - Lifetime
- 1987-07-01 WO PCT/EP1987/000356 patent/WO1988000238A1/de unknown
Also Published As
Publication number | Publication date |
---|---|
JPH07100039B2 (ja) | 1995-11-01 |
WO1988000238A1 (en) | 1988-01-14 |
EP0254090A1 (de) | 1988-01-27 |
ATE89318T1 (de) | 1993-05-15 |
US5268270A (en) | 1993-12-07 |
DE3785798D1 (de) | 1993-06-17 |
EP0254090B1 (de) | 1993-05-12 |
DE3622221A1 (de) | 1988-01-14 |
JPH01500164A (ja) | 1989-01-26 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG2A | Definitive protection |
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